FastQCFastQC Report
Sat 31 Dec 2022
SRR5845342.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845342.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162706
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGACGTGCATCTCGTATGC6080.37368013472152223No Hit
CTTATACACATCTCCGAGCCCACGAGACTGACGTGCATCTCGTATGCCGTCT5950.3656902634199108No Hit
AGCATGGGGATGTGCTGTCTAAGATTTTTAAAGAATAACAGAATAAAGCCTC1940.11923346403943308No Hit
TAGAATGGGAGTGGATAAAATCACAGAAAATTGTCAAATTTCCTCGGGAAAG1800.11062898725308225No Hit
ACGTACGGGATGGACAGATAAACATGTATGAGAGATAGAAATAAGGAAGGAG1740.10694135434464616No Hit
CATTGCGGGAACACAAACAATGACAAAGAAATACAAATGTTCATGCAGTGTA1710.10509753789042814No Hit
ACTTTTGGGAACAGCGAAGGAATTTGCTGATTCCCTTGGAATTCCGTTTTTG1670.10263911595147074No Hit
CATGTCGGGGGCGCGCGGGGCGGTGCCGCGGCGGCGGAGGGAGCCGCGATGG1670.10263911595147074No Hit
ACCAGTGGGAGTTACCAAAATGTAGGCTTTTCTGTCTACTATTTTGGTAATT1650.10140990498199207No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)