FastQCFastQC Report
Sat 31 Dec 2022
SRR5845341.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845341.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162149
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGACGTGCATCTCGTATGC6040.3724969009984644No Hit
CTTATACACATCTCCGAGCCCACGAGACTGACGTGCATCTCGTATGCCGTCT5810.35831241635779437No Hit
AGCATGGGGATGTGCTGTCTAAGATTTTTAAAGAATAACAGAATAAAGCCTC2140.13197737883058175No Hit
GTACTAGGGCAACGCCGACGGCGAGCTGCACCTCAGCCACAATGGCGCGGCC1780.10977557678431567No Hit
GCATGGGCCTTCTTCTGTGGAACTGCCACGCTTGATTTTACTTAAACCACTG1770.10915886006080827No Hit
CATGTCGGGGGCGCGCGGGGCGGTGCCGCGGCGGCGGAGGGAGCCGCGATGG1690.10422512627274913No Hit
GCGTTAGGGACATTTACTTGGCTTAAGTAAAAATAGGTTATTTATGCTTGGC1670.10299169282573435No Hit
TGATCGGGAATATGATGGATGGGAAATCAAGGCTGCTGGAAGGAAGTAGTGT1660.10237497610222697No Hit
TCATATGGGATTCCTGAGTGCAGGGGTAGTCCCTCTCACCTGCTTCCTCAGA1650.10175825937871957No Hit
AATTTCGGGTCACAGAAAAATGGAAAGAAATATGGATGTGCAGAGGTAATAA1630.1005248259317048No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)