FastQCFastQC Report
Sat 31 Dec 2022
SRR5845340.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845340.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162500
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGACGTGCATCTCGTATGCCGTCT5940.36553846153846153No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGACGTGCATCTCGTATGC5940.36553846153846153No Hit
AGCATGGGGATGTGCTGTCTAAGATTTTTAAAGAATAACAGAATAAAGCCTC1790.11015384615384616No Hit
TGATCGGGAATATGATGGATGGGAAATCAAGGCTGCTGGAAGGAAGTAGTGT1760.10830769230769231No Hit
CTCTGTGGGATCAATTTAAATTAGCAAAGAAAGGTGAGAAAAAAATCCCTTT1710.10523076923076922No Hit
GCATGGGCCTTCTTCTGTGGAACTGCCACGCTTGATTTTACTTAAACCACTG1700.10461538461538462No Hit
AAGTGCGGGACACATTCAGAGCAGTGTGTAGAGGGAAATTTATAGCACTAAA1690.104No Hit
CTGAAAGGGATTAATGTCTAGTTCTCAGAGCATTTGGAAAGGTTATCTTAAA1660.10215384615384615No Hit
CCGTGGGGGACAGTATCTAACTTGATCTGAACTGAACCTGTTTTCCTTGATG1650.10153846153846155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)