FastQCFastQC Report
Sat 31 Dec 2022
SRR5845333.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845333.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167357
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGACGTGCATCTCGTATGCCGTCT4970.2969699504651732No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGACGTGCATCTCGTATGC4380.2617159724421446No Hit
GTCATGGGGTCTTCACGTGGGCCGATCGTTTTTTTTTTTTTTTTTTTTTTTT2430.14519858745077888No Hit
TAGTCTGGGCCACAGACATCTGTATCATCAAGCTTTCCCTACACCAAAGTCG2260.13504066157973674No Hit
TGGTTAGGGGAGGAATCAAGGAGAAAAATCCAAATGATTTTCCTTTTTTTCT2250.13444313652849896No Hit
GTGGCCGGGATCTCTTCAAAAAACAGTTTTTCATTTCATTGATCCTTTGTAT2220.13265056137478562No Hit
AATCAAGGGATAGCCAGGGTCAGAAACTTGGTGACAATCACATGAGACCATG2220.13265056137478562No Hit
GCTTTGGGAAGGACAAACCCAGTGCCTATTTCTCAGTCCTTTTTCTACTTGA2180.13026046116983453No Hit
GTCCTTGGGAAGCCTTAAGATATAGGTATTATTTTCTCCCTTTTACAGCTGA2180.13026046116983453No Hit
AACACAGGGTAGAGAGGGAATTGTGTGCCGCGATTTGAACCCAAACAACATC2170.12966293611859678No Hit
TTAATTGGGGGGGGAAGGGGAGGTGGGCAGTAATTAGATATCTCCCAAGAAT2170.12966293611859678No Hit
CACGTAGGGATTTCAAACTTGTTGCCGTGACCAATTTTTACAAAACTGAATG2170.12966293611859678No Hit
CTAAATGGGTGTGGTAAACATGATCAGCAGTCACACTCCTTGTTATTTACCT2150.12846788601612122No Hit
TGTTCAGGGACAGGCTTGAGCCACCGTTCCCAGCCTAGTAATTAATTTTAGA2140.12787036096488347No Hit
ACTCGGGGAAGAAATACAAGAGAAACAGATCTTCAGGGAAGGGAGACAGTAT2130.1272728359136457No Hit
GCCAATGGGACGGAATCTACCATTTTAAAGTTAATTGCTTGTCAAGCTATAA2130.1272728359136457No Hit
GCCATGGGGTAAAAATAAAACGAAAAATACCTTTCAGCAAATACAATCTTTT2130.1272728359136457No Hit
CTCAACGGGGTAGTGTAGCCATGCATTGATCTGTCATCACAGAACTGAAGTC2120.1266753108624079No Hit
TCGTATGGGAGCAGGAGAGAAGGTAGTACTTTGTCCCAAGTTAGATTAGTTT2120.1266753108624079No Hit
TAAGCAGGGAGTGATGTAGAGACCTTGGTTTGCTTTCCCTCCAGTTTCTACC2110.12607778581117013No Hit
TATGGTGGGGCAGTAGGCTCACCCCTAAGGAATCTCATGTTGCCTGCAGTAA2110.12607778581117013No Hit
GTAAAGGGGCATCAAAGAGGGGGAGCGCCTGGAGGTCCGGATGAAACGTCTG2090.12488273570869458No Hit
AATTGAGGGACTGTTTTCCATGGTGGCTGCACTAGTTTATATTCCCACCAAC2090.12488273570869458No Hit
GCGAACGGGTGGAAGAGACAAGCTGACCAATTCTAGACTTTTAGTCCATTTT2090.12488273570869458No Hit
ACGTATGGGAGCCCAGCATGCTTCACTACTCTATGTTATCTTCCTGGCCATT2080.12428521065745682No Hit
ACATCGGGGACACAGTTGAGTTGCAAGCTGAATTGGCTGTGTTCAAGGCATG2080.12428521065745682No Hit
CAAACAGGGCTGTGGAAAGCTAATGCTTCTGAAAAATTGGGTGTGCTTACAT2070.12368768560621904No Hit
ACTAATGGGGATATTCAGACCTTGACTTTGAGAAGGTCATGCATACAAGGAA2070.12368768560621904No Hit
TTTTAGGGGGTCTCGTCCTGCCCGCACTGCCTCTCCCAGGAACTGGAAAAGC2070.12368768560621904No Hit
CTCCTGGGGCAGGCAGTCAGACCTTATGGTCGTCTTCCCTTGTTCCCTAAAA2060.12309016055498127No Hit
CTTCTAGGGATTCACTTTACAAATATGTATTGAATTCACACTATGCTGAAGT2050.1224926355037435No Hit
CAATTCGGGAAGGATTTCAAGGAGCAGGTGATACACCACTTCGTGGCGGTCA2040.12189511045250573No Hit
TCAGTCGGGTCCTTCCGTGACTGGTCACGTTGTCTGCCTGGGCTCAGCGTGG2040.12189511045250573No Hit
TGTCGCGGGAGCACAGTTGCAGCATACAAAATCAATAAACAAAAATCAATGA2040.12189511045250573No Hit
TGCCTTGGGGACCCCGGAGGCCGGCGCGGACAAGCGGAGCCCACGGCGGGCC2030.12129758540126793No Hit
GACTTGGGGCATACCTTTACTGAATGAGAATTCTTTAATTTAAAAAATTGCT2010.12010253529879239No Hit
CATTCAGAGGAAATAAAACTTGCCTGATTGGACTTTCTTTCCCCTGATAGAA2010.12010253529879239No Hit
GTCTGGGGAATGGTTCTAGATGTCCCAAGAAATCCTAGACCCCTTGTATCCA2000.11950501024755462No Hit
TTGTTTGGGGGGTTATATTGCAAATGATCTGTACTTTAGGGTAGAATTCAAT1980.11830996014507908No Hit
TTTGCAGGGAAATGATGACAGCAAAGTGAAGACCTTGTACAGAATGTGTTAA1980.11830996014507908No Hit
CACACGGGATCGAAGTATATTGATGTATTCTAAAGCTAGTAAACTTCCCTAA1980.11830996014507908No Hit
GTAGCCGGGCAGCGGCCTCCTTACGGTTACTACTGATAGAAATGTTGGCAGC1970.11771243509384131No Hit
AGTCAAGGGATGTAGGGGTCAGGTAATGTTCTAACTTCTGCTTTTTCCTAAG1970.11771243509384131No Hit
TGTTTAGGGGGTTCTCATGCTGGCAACAAGCTGGCCATGCAGGAGTTCATGA1950.11651738499136577No Hit
GATCTTGGGGTGCCTCATCGCGGCCGAGGCAGGCCTTTCCGAGGAGGAGACC1950.11651738499136577No Hit
TTTTGCGGGGGAGAAGTAAAAATATGTCTTAAAAAATCATTGATGTTATAGT1940.11591985994012799No Hit
GCATTAGGGGACCTGAGAAAACGAGCCTGTTTCAGAGGAACATCGTCACAAC1940.11591985994012799No Hit
CTTGTGGTGGGAAATGAGAAGACTGTAGCTACTTAGAGTGTATGCTTGTAAT1940.11591985994012799No Hit
TAGTTTGGGCATTTTCCGAATATACAGCTTGGGCAATGGTAGATGGTGGTTC1920.11472480983765244No Hit
GGCATAGGGGCCAAATCTCTCACCAGATAAGTCCTTTTCTCCCTGTGCCGTT1910.11412728478641468No Hit
TATCAAGGGCAGTCTGAATGCTGCCACAACCAGCCAGGCAGGTCCACAGAGA1910.11412728478641468No Hit
AAAGCCGGGTATGCCTTCGTGAAGCTCAAGGAGCAGGAGTGTCGCAACATCG1900.11352975973517689No Hit
GAAAGGGGGCATGCTTTAACAATTTATTACTTTTAAATCTAGAGTGAATTCT1900.11352975973517689No Hit
GCAAACGGGCTGGTAAGGTTAAAATAGGGGAGAAATCCTACATGTTGGAATG1900.11352975973517689No Hit
GTTTCTGGGGTGAAGGGAAATGAGGGCTTTAGTTTCCTGGGCAGGGAGGGGC1900.11352975973517689No Hit
TACGTAGGATCAACAGAAATAAAAGGAAATAATAAATACATGCATAGTGATT1890.11293223468393913No Hit
AGAATTGGGTTCTCTCATCTAGGTTCGTGCACTTAACAAACCTATCGTGATA1890.11293223468393913No Hit
ATCTGCAGGGCGATCTCATAGCGGGATAATACATACTTTTCCACAAAGCATG1890.11293223468393913No Hit
ATGCAGGGTATGCAAAATGTTTTCCTCCCCAATGTAGAGATATAAGCCAGTT1890.11293223468393913No Hit
CTGTGAGCGGGTTTATTCTGCCTTATTTCCTTCACCATTGTCACCTTTGTTA1880.11233470963270134No Hit
GTACCGGGGGCGTGGGGAGCCTTGTCGGAGAGAGCTGTGGCAGTGGTTGTGG1880.11233470963270134No Hit
AAATCAGGGGATCACCAAGACAGAAAGTCAACAAAGAAACAATGGACTTAAA1880.11233470963270134No Hit
CGAAAAGGGGGGCTGAATGCAGCCATCTGAGCAAGGGCTGAGGGAAGCCATC1880.11233470963270134No Hit
ATGCAGGGAATAACATTTTTAAATGAAAATTAACTATTGAAAACAAAAATTT1870.11173718458146358No Hit
TTGATCGGGGGTTAGCCTGAAGTCTGAATGGAGCCCATAGTTGGAAAACAAC1870.11173718458146358No Hit
GCTGCAGGGTCCAGATGAAAAAAATAGAGTGCGCAACTTCCTTTTAAGGCAT1870.11173718458146358No Hit
TATTGCGGGGTTGCCATTAATGTGCTGCATGATCTTGGGAAAATCATTTACC1860.1111396595302258No Hit
TTGCTGGGGGCATGCTTTTGGTTTGTTACCAAAATATACAGTGTGGTGAAGG1860.1111396595302258No Hit
AATGCCGGGGCAGCAATTGAAAGGGAATTTCAGTACTTTTATAGAATTCTTA1860.1111396595302258No Hit
GTTAGTGGGGTTCTTGAGGAAAGCCGGTGGAAGATTCTGGAATGCCGTGCGG1860.1111396595302258No Hit
AATGCAGGGAGGGTCAGCACTGGGTCAGCGACTCCAATCTTCCAGTGGCCAG1860.1111396595302258No Hit
TGGATAGGGTCTGGAATTCTTTGGTGTCTAAGTGCATGGTAGTTTTTTTTTT1850.11054213447898803No Hit
CGTAGCGGGTCCAATGCAACTCTAACTGCATACTTCTGAAATGTTTACCCCC1850.11054213447898803No Hit
AACACTGGAGTCTCATAATTTGGAATTTAAGATAATAAGTAGTAACAAGGCA1840.10994460942775025No Hit
CTAAGGGAATGGACACATCAAAAGCGAGCAGGAGTAGCTGTTCTTATATCAG1840.10994460942775025No Hit
AAGCTGGGTGTTGGACTTTTAAAAAACCAGTTGGAAATATAGGTTGGGTTTT1840.10994460942775025No Hit
TGCCGCGGGAAGAGCCCTCTGGCACAAATGGAAGAAGAAAGAAGGGAGCATG1840.10994460942775025No Hit
TGTTTAGGGGTTTTAAATTAGCTGCTACTAGAATGTTTTGATCAAAATGAAA1830.10934708437651249No Hit
TTTACTGGGGAATGACTTCATAGTGATTTGGAAATTTAATTTGCCAAAGCTC1830.10934708437651249No Hit
AACTGAGGGATTGAATATTTGTACTGATAAAAATGCAACCCAATCCATACCT1820.10874955932527472No Hit
GTTCTAAGGGCAGTGGCCTGATCTCGGCTCACTGCAACCTTCTCCTCCCAGG1810.10815203427403694No Hit
GAAGTCGGGGATGAATTACTAATGCCTTATTTTCTAGGCATATAATAATAGT1810.10815203427403694No Hit
CTGTCTGGGACCGAGCTGTGGAGTTCCTGGCCTCCAACGAATCCCGGATCCA1810.10815203427403694No Hit
TTAGCAGGGAGAACAGTTTTATAGATAAAAAGATAATCATAGTTGCTATAGT1810.10815203427403694No Hit
GAAACGGGGAAGCTTATTTCAGAACAAGTCTAATATTTCAGATTCTTTCTTT1810.10815203427403694No Hit
CTCTGTGGGATGAAAATAATCAGCAGTAAATTGAACCTGCATAAAAAATTCT1800.10755450922279917No Hit
AGTTCCCATGACTCCTGGATCTATGTGTATGCAGAAGGCTACATATACATAG1790.1069569841715614No Hit
GTTCCAGGGGGTTTTATTTACATTTTAGGGTGGGTAAGAAAGCCACCTTGTT1790.1069569841715614No Hit
CTTGACGGGGAGTCGGAGCCAGCGCGAGCCGCCGCCGCCATCACTGCCGCTG1790.1069569841715614No Hit
CTAAGGACGGGTATGTCAAAGTCTAACTTCTTCTTTTTTTGTTTTTATTTTT1790.1069569841715614No Hit
TGTCAGGGGATATTATCAGCGGTTATTCGGTGAGCGGTGGTGGTTTATTCTT1790.1069569841715614No Hit
ATAATAGGGTGCATGCAGAGAGAATGTCTTCATAGAGAGAATGTCATTAAAT1780.10635945912032363No Hit
GCAGCCTCTCTTATACCCCTGATATTTCATTCTTAGTACTAGATGAGTATAT1780.10635945912032363No Hit
GTAGTAGGGATATTTGAACATCTGTGTTCATAGTGGCATTATTCACAATGGC1780.10635945912032363No Hit
CTGTGAGCGGGACCGCCTTGCCCAGAAGAAGCCCTTCACTCCGGTCAAGTAC1780.10635945912032363No Hit
GTCACCGGGATTTGTCTAATTGCAAGGCAAGTCAAGCTCTAAAACAATTGCT1750.10456688396661029No Hit
TTTCTGGGCACGTTTCAAGAAATGTTAAGCTCTTCTTGGAACAAGCCCCACC1750.10456688396661029No Hit
TTCTATGGGTCTGAATGGGGGATACCAATCTCACCATAAACTTACAGCTAAC1750.10456688396661029No Hit
CTGCGGGGGTACCATGGACGGAAGCTATCGGTGCAGGGCTTCAAGGAGGCAC1750.10456688396661029No Hit
GCAGCAGGGCATACAAAATTTATTACTGTTTGCCATATTTATAGCAAACATA1740.10396935891537254No Hit
ATGCACGGGGTGGCTTTTGATGGGGCATTTTGTCCTTCTAAGCACCTATGGA1740.10396935891537254No Hit
AAGTCTGGGATATAAAATTTAAAGTAGTTTTGTCTACTTCTGTAAAGAATGT1740.10396935891537254No Hit
GTTAGAGGGAGAGGAGCTGTCCGTGTGCTGAAACGGCCCGAGAAGCTCGCCC1740.10396935891537254No Hit
CTGCCATGGGGTCTATAATGCCCTTCTATATGTGCTACCATAGATGTGACAT1740.10396935891537254No Hit
CGTGGCGGGTAGTATTGTTTATCAGAAAGAGGAAGTGTACTTAAGATTATTT1740.10396935891537254No Hit
TCAGCTGGGAGACGGGTCAGTGGGAATAACGGGCCAACAAGACAGCTTTTTA1740.10396935891537254No Hit
TAGGACGGGATCATCGTGTTTGTAAGAGAAGATGGTCATTTTATTCTCTGTA1740.10396935891537254No Hit
CTTCATGGGAGAGCCAAGTTTAACGAGGTTTTCCTTTGACACCCTGAGAGCC1730.10337183386413475No Hit
TAAGCCAGGGATTTATTGCATTGGTGTTTTCACCCAATTGTTAAGTTTGATA1730.10337183386413475No Hit
ACACCTGGGTAAACAGAACCACTACCAAGAGCTCAATGACATCCTCAACCTG1730.10337183386413475No Hit
TAAATGGGGTTTGAATGAGTCAGCAGACATAACTTTTGCCACTTTAAATACT1730.10337183386413475No Hit
TTGCGAGCGGGGATAGCTGTGCTCAGTGATGGTACGTTAAATTTCACAAATG1720.10277430881289698No Hit
GCACTTGGGATAATAAATAGGCTACAGCATGGGGAACAAGCTGAGGGTACTA1720.10277430881289698No Hit
AGATAGGGGTGATTGGGGGTGCGGTGGCTAGTAATTACTGCCATTATTGTCT1720.10277430881289698No Hit
TAGCATGGGGGTAGTAAAATCCTTAATGAAACCAGTGGATGTGCTTAACGTA1720.10277430881289698No Hit
TGTACAGGGCACTACCTATTCTGACAACCAGCCTGGTGTGCTTATTCAGGTT1710.1021767837616592No Hit
GACTTCGGGTGTTGTGATTTATGAAAATTAACAATCTGAACAAGAGAAGGAA1710.1021767837616592No Hit
TGCAATGGGATTCTTTAAAGTTTTTAACATAAGCTTGTCAATGCACATGTAG1700.10157925871042144No Hit
AATACGGGGAGCAGAGTCGGGGCTTTGGCAGCTTCAGGTAAGTTGGTTGGGA1700.10157925871042144No Hit
TCACCGGGGGGGGATATTCAATGACAGTTGGATGTCTTTGATTTTTTTTCCA1700.10157925871042144No Hit
AAACAGGGGAAGCTTTTGAACAACAAAAGCCAAAAAGAACTGAACTCTGCTT1700.10157925871042144No Hit
AGATTGGGCAGATTTCAAGAGGAATCGCCTGGCCGAGCCTTTTAACAGCATC1700.10157925871042144No Hit
TATTATGGGCCTGTCCACCTGTCACCAGCACAAGAGAATTAGAGCTTCAGTG1700.10157925871042144No Hit
ATGACTGGGAGCTCTGCTTAGGGACAGACTGCCTCCTCAAGTGAGTTGAGGA1700.10157925871042144No Hit
TATTCTGGGGTGGTAGACACTCTTGAGCTGGACTTAGATTTTATTCTTCCTT1700.10157925871042144No Hit
GGGTTCGGGTAATCTGAACTTCAGACTGTAGTCTCATCCAAAAATGTTAACA1690.10098173365918366No Hit
TGAGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1690.10098173365918366No Hit
TTGCCAGGGTTGCTGATAGTAAGTACCCTTAATCTATTAAAAGAAATACAAA1690.10098173365918366No Hit
ATTTCTGGGGACACACTGAACCCAGCTTCTCAGCAGTGAGCAGCGCGCTCAG1680.10038420860794589No Hit
ACAGTAGGGGCTTCTCGCCTAACGCTGCCAACATGGTGTTCAGGCGCTTCGT1680.10038420860794589No Hit
TTTCCAGGGATCCCTGACATCTGTATCTTGGATTACTCCAAATGGAACAGTC1680.10038420860794589No Hit
AGAATGGGGTAGCTTTTGAAGAAAGGCCAGTGATCCAGCAAAGGGGGAAAAA1680.10038420860794589No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)