FastQCFastQC Report
Sat 31 Dec 2022
SRR5845332.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845332.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166800
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGACGTGCATCTCGTATGCCGTCT5300.3177458033573141No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGACGTGCATCTCGTATGC4520.2709832134292566No Hit
TATCAAGGGCAGTCTGAATGCTGCCACAACCAGCCAGGCAGGTCCACAGAGA2440.14628297362110312No Hit
TGCCGCGGGAAGAGCCCTCTGGCACAAATGGAAGAAGAAAGAAGGGAGCATG2380.14268585131894485No Hit
GTCATGGGGTCTTCACGTGGGCCGATCGTTTTTTTTTTTTTTTTTTTTTTTT2340.14028776978417265No Hit
TTAATTGGGGGGGGAAGGGGAGGTGGGCAGTAATTAGATATCTCCCAAGAAT2340.14028776978417265No Hit
GCTTTGGGAAGGACAAACCCAGTGCCTATTTCTCAGTCCTTTTTCTACTTGA2290.1372901678657074No Hit
TGTCGCGGGAGCACAGTTGCAGCATACAAAATCAATAAACAAAAATCAATGA2210.13249400479616308No Hit
TGTTTAGGGGTTTTAAATTAGCTGCTACTAGAATGTTTTGATCAAAATGAAA2210.13249400479616308No Hit
TGGATAGGGTCTGGAATTCTTTGGTGTCTAAGTGCATGGTAGTTTTTTTTTT2200.13189448441247004No Hit
TCAGTCGGGTCCTTCCGTGACTGGTCACGTTGTCTGCCTGGGCTCAGCGTGG2180.13069544364508393No Hit
GTAAAGGGGCATCAAAGAGGGGGAGCGCCTGGAGGTCCGGATGAAACGTCTG2180.13069544364508393No Hit
AATCAAGGGATAGCCAGGGTCAGAAACTTGGTGACAATCACATGAGACCATG2150.1288968824940048No Hit
CATTCAGAGGAAATAAAACTTGCCTGATTGGACTTTCTTTCCCCTGATAGAA2150.1288968824940048No Hit
AATGCCGGGGCAGCAATTGAAAGGGAATTTCAGTACTTTTATAGAATTCTTA2140.12829736211031176No Hit
GACTTGGGGCATACCTTTACTGAATGAGAATTCTTTAATTTAAAAAATTGCT2130.12769784172661872No Hit
AACACAGGGTAGAGAGGGAATTGTGTGCCGCGATTTGAACCCAAACAACATC2110.1264988009592326No Hit
CTGCCATGGGGTCTATAATGCCCTTCTATATGTGCTACCATAGATGTGACAT2110.1264988009592326No Hit
GATCTTGGGGTGCCTCATCGCGGCCGAGGCAGGCCTTTCCGAGGAGGAGACC2110.1264988009592326No Hit
TTTACTGGGGAATGACTTCATAGTGATTTGGAAATTTAATTTGCCAAAGCTC2100.12589928057553956No Hit
GTCTGGGGAATGGTTCTAGATGTCCCAAGAAATCCTAGACCCCTTGTATCCA2090.12529976019184652No Hit
CTCAACGGGGTAGTGTAGCCATGCATTGATCTGTCATCACAGAACTGAAGTC2090.12529976019184652No Hit
TGGTTAGGGGAGGAATCAAGGAGAAAAATCCAAATGATTTTCCTTTTTTTCT2080.12470023980815348No Hit
CACGTAGGGATTTCAAACTTGTTGCCGTGACCAATTTTTACAAAACTGAATG2080.12470023980815348No Hit
AGAATTGGGTTCTCTCATCTAGGTTCGTGCACTTAACAAACCTATCGTGATA2070.12410071942446042No Hit
CGAAAAGGGGGGCTGAATGCAGCCATCTGAGCAAGGGCTGAGGGAAGCCATC2060.1235011990407674No Hit
GCCATGGGGTAAAAATAAAACGAAAAATACCTTTCAGCAAATACAATCTTTT2060.1235011990407674No Hit
ATGACTGGGAGCTCTGCTTAGGGACAGACTGCCTCCTCAAGTGAGTTGAGGA2050.12290167865707433No Hit
TGCCTTGGGGACCCCGGAGGCCGGCGCGGACAAGCGGAGCCCACGGCGGGCC2050.12290167865707433No Hit
GTCCTTGGGAAGCCTTAAGATATAGGTATTATTTTCTCCCTTTTACAGCTGA2050.12290167865707433No Hit
TATGGTGGGGCAGTAGGCTCACCCCTAAGGAATCTCATGTTGCCTGCAGTAA2040.1223021582733813No Hit
CAAACAGGGCTGTGGAAAGCTAATGCTTCTGAAAAATTGGGTGTGCTTACAT2040.1223021582733813No Hit
CATGCAGGGCGATCTCTGATCGGGAGAGTGGGGAGCAACAGTTTGATTTTCT2040.1223021582733813No Hit
GTTCTAAGGGCAGTGGCCTGATCTCGGCTCACTGCAACCTTCTCCTCCCAGG2020.1211031175059952No Hit
GGGTTCGGGTAATCTGAACTTCAGACTGTAGTCTCATCCAAAAATGTTAACA2020.1211031175059952No Hit
TCGTATGGGAGCAGGAGAGAAGGTAGTACTTTGTCCCAAGTTAGATTAGTTT2020.1211031175059952No Hit
AAGTCTGGGATATAAAATTTAAAGTAGTTTTGTCTACTTCTGTAAAGAATGT2010.12050359712230216No Hit
ATGCAGGGTATGCAAAATGTTTTCCTCCCCAATGTAGAGATATAAGCCAGTT2010.12050359712230216No Hit
CTGTATGGGGAAAGCTAACCGTCTAGAACCAAAGCTTACAAAAATCTCTCAC2000.1199040767386091No Hit
TGTTTAGGGGGTTCTCATGCTGGCAACAAGCTGGCCATGCAGGAGTTCATGA2000.1199040767386091No Hit
CTAAGGACGGGTATGTCAAAGTCTAACTTCTTCTTTTTTTGTTTTTATTTTT2000.1199040767386091No Hit
TTGATCGGGGGTTAGCCTGAAGTCTGAATGGAGCCCATAGTTGGAAAACAAC2000.1199040767386091No Hit
AATGCAGGGAGGGTCAGCACTGGGTCAGCGACTCCAATCTTCCAGTGGCCAG2000.1199040767386091No Hit
GAAGTCGGGGATGAATTACTAATGCCTTATTTTCTAGGCATATAATAATAGT1990.11930455635491607No Hit
TTTTAGGGGGTCTCGTCCTGCCCGCACTGCCTCTCCCAGGAACTGGAAAAGC1990.11930455635491607No Hit
CTGTGAGCGGGACCGCCTTGCCCAGAAGAAGCCCTTCACTCCGGTCAAGTAC1990.11930455635491607No Hit
GAAAGGGGGCATGCTTTAACAATTTATTACTTTTAAATCTAGAGTGAATTCT1980.11870503597122303No Hit
TTTGGCGGGATTCCTCCAAGTGGAAATTTGGACATTACCCTTTGTTCATGGT1980.11870503597122303No Hit
GCATTAGGGGACCTGAGAAAACGAGCCTGTTTCAGAGGAACATCGTCACAAC1980.11870503597122303No Hit
TTGTTTGGGGGGTTATATTGCAAATGATCTGTACTTTAGGGTAGAATTCAAT1970.11810551558752998No Hit
AATTGAGGGACTGTTTTCCATGGTGGCTGCACTAGTTTATATTCCCACCAAC1970.11810551558752998No Hit
TAGTCTGGGCCACAGACATCTGTATCATCAAGCTTTCCCTACACCAAAGTCG1970.11810551558752998No Hit
CGCTTTGGGGCCTATAGTTAAAATATAGACAAAGTATAATAAAATGCTTAGG1950.11690647482014388No Hit
CTAAATAGGGACTGGGGTGGGGTAGCTTTCGGGAAAGGGAATAAGGAGCACG1950.11690647482014388No Hit
CTAGGGGGCGCATATGTGACTGTCTTCCCCTAGACTGATGGTATACTTTTGA1950.11690647482014388No Hit
TTGCCAGGGTTGCTGATAGTAAGTACCCTTAATCTATTAAAAGAAATACAAA1950.11690647482014388No Hit
CGATTAGGGAATCCTTTACTTTGAGGCTTTTTGTCTGCTAAAAATGTTGACG1940.11630695443645084No Hit
TAGCATGGGGGTAGTAAAATCCTTAATGAAACCAGTGGATGTGCTTAACGTA1940.11630695443645084No Hit
TGTTCAGGGACAGGCTTGAGCCACCGTTCCCAGCCTAGTAATTAATTTTAGA1930.11570743405275778No Hit
GTAGCCGGGCAGCGGCCTCCTTACGGTTACTACTGATAGAAATGTTGGCAGC1930.11570743405275778No Hit
ACATCGGGGACACAGTTGAGTTGCAAGCTGAATTGGCTGTGTTCAAGGCATG1920.11510791366906474No Hit
GTTTCTGGGGTGAAGGGAAATGAGGGCTTTAGTTTCCTGGGCAGGGAGGGGC1920.11510791366906474No Hit
CTAAATGGGTGTGGTAAACATGATCAGCAGTCACACTCCTTGTTATTTACCT1900.11390887290167866No Hit
GGCTACAGTTTTGGTGGGATGGGCAGTTCAGGTGTCCTGCCAACGAGATACT1900.11390887290167866No Hit
CACACGGGATCGAAGTATATTGATGTATTCTAAAGCTAGTAAACTTCCCTAA1900.11390887290167866No Hit
ATCTGCAGGGCGATCTCATAGCGGGATAATACATACTTTTCCACAAAGCATG1890.11330935251798561No Hit
TGTACAGGGCACTACCTATTCTGACAACCAGCCTGGTGTGCTTATTCAGGTT1890.11330935251798561No Hit
TATTCTGGGGTGGTAGACACTCTTGAGCTGGACTTAGATTTTATTCTTCCTT1890.11330935251798561No Hit
GTACCGGGGGCGTGGGGAGCCTTGTCGGAGAGAGCTGTGGCAGTGGTTGTGG1880.11270983213429256No Hit
TTAGCAGGGAGAACAGTTTTATAGATAAAAAGATAATCATAGTTGCTATAGT1880.11270983213429256No Hit
AAATCAGGGGATCACCAAGACAGAAAGTCAACAAAGAAACAATGGACTTAAA1870.11211031175059952No Hit
ACGTATGGGAGCCCAGCATGCTTCACTACTCTATGTTATCTTCCTGGCCATT1870.11211031175059952No Hit
CTCTCTTCGGGAAGGCTGAAGGGATTGGTGTCAATCAAAGACAAATCACAAC1860.11151079136690649No Hit
AGTAATGGGCAATAAAACTTTATTTGCAGAATCCGGCAGTGACTGGCTTTAG1860.11151079136690649No Hit
CGTGGCGGGTAGTATTGTTTATCAGAAAGAGGAAGTGTACTTAAGATTATTT1860.11151079136690649No Hit
GGAACTGGGGATCAGCTACCACTTATCATTGTGTGTGATCGATTTGACTTTG1850.11091127098321342No Hit
AAATAAGGGGCTTTTCCGCCCGCTCCCCCCTCCCCCCGAGCGCCGCTCCGGC1850.11091127098321342No Hit
CTTCTAGGGATTCACTTTACAAATATGTATTGAATTCACACTATGCTGAAGT1850.11091127098321342No Hit
GTTTGCGGGGCCTGCTTATATATGAGTTAAGTAAATAAAAATGAAGGTTTTT1850.11091127098321342No Hit
GCTGTTGGGGTGGAGTGATCCTGGATATACATTTTCTCTCTCAGGCAGACTT1850.11091127098321342No Hit
AATAGTGGGCGATCTGATGGTGGGGCACCCCAGTCGGCACAGTGCATTTGAC1840.11031175059952039No Hit
GGGCTTGGGTACCTCAGGCCTGATCCATCGTGCCCTTGACTTTGCCGTCTCA1830.10971223021582734No Hit
ACTAATGGGGATATTCAGACCTTGACTTTGAGAAGGTCATGCATACAAGGAA1830.10971223021582734No Hit
ACTCGGGGAAGAAATACAAGAGAAACAGATCTTCAGGGAAGGGAGACAGTAT1820.1091127098321343No Hit
GCATTTGGGTGAAAAGTGATGTGTAGATGAAAACACAAGGTATGGATGTTGG1820.1091127098321343No Hit
AAAAATGGGGTCTTATTAATCACCAGAAAATGGTCATATCAAATGTTCTTTC1810.10851318944844125No Hit
TAGTTTGGGCATTTTCCGAATATACAGCTTGGGCAATGGTAGATGGTGGTTC1810.10851318944844125No Hit
TAGGACGGGATCATCGTGTTTGTAAGAGAAGATGGTCATTTTATTCTCTGTA1810.10851318944844125No Hit
ATGACCTGGGCCACTGTGACAAAATCAGGTTTATTACCTCAGTTTAATGAAG1810.10851318944844125No Hit
ATGCAGGGAATAACATTTTTAAATGAAAATTAACTATTGAAAACAAAAATTT1810.10851318944844125No Hit
AGATTGGGCAGATTTCAAGAGGAATCGCCTGGCCGAGCCTTTTAACAGCATC1800.1079136690647482No Hit
CTGCGGGGGTACCATGGACGGAAGCTATCGGTGCAGGGCTTCAAGGAGGCAC1800.1079136690647482No Hit
CTGTCTGGGACCGAGCTGTGGAGTTCCTGGCCTCCAACGAATCCCGGATCCA1800.1079136690647482No Hit
AGTCAAGGGTTCCTTTTAATGTTTCCCTGCTATGCAAAACCTTTCCCAGACC1800.1079136690647482No Hit
ACACCTGGGTAAACAGAACCACTACCAAGAGCTCAATGACATCCTCAACCTG1790.10731414868105515No Hit
GTTCATGGGGCTGAGGTATGGCTAAGGTATTCTGTCAGGTTCTTCCAAATAC1790.10731414868105515No Hit
TACGTAGGATCAACAGAAATAAAAGGAAATAATAAATACATGCATAGTGATT1780.1067146282973621No Hit
TATGGCGGGGGAACGGGAGATGTTGCAGGCGCCGAGAGGGCGGGCCAGGGCC1780.1067146282973621No Hit
CACATGGGGAGAGGTGTCCTGCAGACCGGCTGACCACTCCTTTTGGAGACGG1780.1067146282973621No Hit
CTAAGGGAATGGACACATCAAAAGCGAGCAGGAGTAGCTGTTCTTATATCAG1770.10611510791366907No Hit
GTGGCCGGGATCTCTTCAAAAAACAGTTTTTCATTTCATTGATCCTTTGTAT1770.10611510791366907No Hit
AAACAAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAAACCACAAA1770.10611510791366907No Hit
CACTACGGGGTGTTAAAAAGCACTAAAAGCAAATGTTCATAATTTCCATTCA1770.10611510791366907No Hit
GCAGCAGGGCATACAAAATTTATTACTGTTTGCCATATTTATAGCAAACATA1760.105515587529976No Hit
TTCTCCGGGGCACACACATTGAAGTAAAATGAAAGGAATAACCATGTAGTCA1760.105515587529976No Hit
GTTCCAGGGGGTTTTATTTACATTTTAGGGTGGGTAAGAAAGCCACCTTGTT1760.105515587529976No Hit
CCTTAAACACCCAGCCACGGCCAGGGGCTTGTTGAGGTTAATGAGTTTCTGT1760.105515587529976No Hit
AACACTGGAGTCTCATAATTTGGAATTTAAGATAATAAGTAGTAACAAGGCA1750.10491606714628297No Hit
TAAGCAGGGAGTGATGTAGAGACCTTGGTTTGCTTTCCCTCCAGTTTCTACC1750.10491606714628297No Hit
AGGCCTGGGAGCAGAGACATGCAACAACAACACACACGCACACACGCACACA1750.10491606714628297No Hit
ATTTCTGGGGACACACTGAACCCAGCTTCTCAGCAGTGAGCAGCGCGCTCAG1740.10431654676258993No Hit
TATCCTGGGATGCCTTGCTGTTTTTATGTGAAATTTATTTTAAAGGACTTAC1740.10431654676258993No Hit
GCGAACGGGTGGAAGAGACAAGCTGACCAATTCTAGACTTTTAGTCCATTTT1740.10431654676258993No Hit
AAAGCCGGGTATGCCTTCGTGAAGCTCAAGGAGCAGGAGTGTCGCAACATCG1730.10371702637889688No Hit
TAAGTAAGGGACTTGATGATCTGTAAGGGAACTTGCTGATTTGGGAATGTGC1730.10371702637889688No Hit
GCAGCCTCTCTTATACCCCTGATATTTCATTCTTAGTACTAGATGAGTATAT1730.10371702637889688No Hit
GCTACTGGGGCTGAAGAAACATGAGCATCTGCTTTAAAATTAATAGTTAATT1730.10371702637889688No Hit
CAAGAGGGGTGAACTGGTTATGGAAGGTGACTTTGGGATGTAAGAGTGTTCA1730.10371702637889688No Hit
ATCCTTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1730.10371702637889688No Hit
AACTGAGGGATTGAATATTTGTACTGATAAAAATGCAACCCAATCCATACCT1730.10371702637889688No Hit
GTTAGTGGGGTTCTTGAGGAAAGCCGGTGGAAGATTCTGGAATGCCGTGCGG1730.10371702637889688No Hit
ATACAAGGGATGAAGAGGCAGCTGATGCCATGCACTTGTCTGAGGGCATAGC1720.10311750599520383No Hit
CTTGTGGTGGGAAATGAGAAGACTGTAGCTACTTAGAGTGTATGCTTGTAAT1720.10311750599520383No Hit
ATGCACGGGGTGGCTTTTGATGGGGCATTTTGTCCTTCTAAGCACCTATGGA1710.10251798561151078No Hit
ATTGTTGGGATGGATTCTCAAGGATAGACAATATGTTAGGCCACAAACACGT1710.10251798561151078No Hit
ACGAAGGGGAACTCCAATGTCACAGAAAAGAAAAGCAGAGTCAGCTACTGAG1710.10251798561151078No Hit
TGCAATGGGATTCTTTAAAGTTTTTAACATAAGCTTGTCAATGCACATGTAG1700.10191846522781775No Hit
CAATTCGGGAAGGATTTCAAGGAGCAGGTGATACACCACTTCGTGGCGGTCA1700.10191846522781775No Hit
CTTCATGGGAGAGCCAAGTTTAACGAGGTTTTCCTTTGACACCCTGAGAGCC1700.10191846522781775No Hit
TCTGCTAGGGCGATCTCATAATGGGGCTCACCAACACATCTTTTTTATGATA1700.10191846522781775No Hit
AGCGCTGGGCAGGGTCTTTGCTATACAAAAGTGTTTTCCACTAATTTTGCAT1700.10191846522781775No Hit
TTTTGCGGGGGAGAAGTAAAAATATGTCTTAAAAAATCATTGATGTTATAGT1700.10191846522781775No Hit
GCTGCAGGGTCCAGATGAAAAAAATAGAGTGCGCAACTTCCTTTTAAGGCAT1700.10191846522781775No Hit
GCTGCTGGGTAATATATTTATTTAAAAAGCTGTGAATAATAAGAATCAAATT1700.10191846522781775No Hit
TAAATGGGGTTTGAATGAGTCAGCAGACATAACTTTTGCCACTTTAAATACT1700.10191846522781775No Hit
TCAACCGGGCATTATATTTGCATAAATGTGTTCCAAAATTGTATGAGATTCT1700.10191846522781775No Hit
GCAAACGGGCTGGTAAGGTTAAAATAGGGGAGAAATCCTACATGTTGGAATG1690.10131894484412471No Hit
GAAACGGGGAAGCTTATTTCAGAACAAGTCTAATATTTCAGATTCTTTCTTT1690.10131894484412471No Hit
CTTGACGGGGAGTCGGAGCCAGCGCGAGCCGCCGCCGCCATCACTGCCGCTG1680.10071942446043165No Hit
CAATTGGGGGTATAAAACAGCTGAATGAATGACAAAAATATTGGCAAGCTCT1680.10071942446043165No Hit
GTTAGAGGGAGAGGAGCTGTCCGTGTGCTGAAACGGCCCGAGAAGCTCGCCC1680.10071942446043165No Hit
TATTATGGGCCTGTCCACCTGTCACCAGCACAAGAGAATTAGAGCTTCAGTG1680.10071942446043165No Hit
ACCTTTGTGACCTCATCATCTGCCAGTCTTTTCCCTTTCTTTCATAAGGGCA1680.10071942446043165No Hit
CGTTGCGGGGAACCAAGGCAATGCTGCTGACTTTGATATGTATGACTCAGTC1680.10071942446043165No Hit
TTCTTGGGGTACATAGACAATATTAAATTTCTTCATACTAGAATAATAAAAG1670.10011990407673861No Hit
TATTGCGGGGTTGCCATTAATGTGCTGCATGATCTTGGGAAAATCATTTACC1670.10011990407673861No Hit
GCCAATGGGACGGAATCTACCATTTTAAAGTTAATTGCTTGTCAAGCTATAA1670.10011990407673861No Hit
GCCGAGGGATTTATATATGAGTAGAGTACAACATATACTAGAGGCCAAGGCC1670.10011990407673861No Hit
CAAGCTGGGGTCTCAGTATCCCCATCTGTAAAATTGTTGTAGAGAAGAATTT1670.10011990407673861No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)