FastQCFastQC Report
Sat 31 Dec 2022
SRR5845331.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845331.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165722
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGACGTGCATCTCGTATGCCGTCT4700.28360748723766305No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGACGTGCATCTCGTATGC4570.27576302482470644No Hit
AATGCCGGGGCAGCAATTGAAAGGGAATTTCAGTACTTTTATAGAATTCTTA2400.14482084454689179No Hit
TATGGTGGGGCAGTAGGCTCACCCCTAAGGAATCTCATGTTGCCTGCAGTAA2330.14059690324760743No Hit
CTCAACGGGGTAGTGTAGCCATGCATTGATCTGTCATCACAGAACTGAAGTC2300.1387866426907713No Hit
GACTTGGGGCATACCTTTACTGAATGAGAATTCTTTAATTTAAAAAATTGCT2240.13516612157709898No Hit
GAAAGGGGGCATGCTTTAACAATTTATTACTTTTAAATCTAGAGTGAATTCT2200.1327524408346508No Hit
CAAGCTGGGGTCTCAGTATCCCCATCTGTAAAATTGTTGTAGAGAAGAATTT2170.13094218027781465No Hit
GCTTTGGGAAGGACAAACCCAGTGCCTATTTCTCAGTCCTTTTTCTACTTGA2160.1303387600922026No Hit
TGTTCAGGGACAGGCTTGAGCCACCGTTCCCAGCCTAGTAATTAATTTTAGA2160.1303387600922026No Hit
AACACAGGGTAGAGAGGGAATTGTGTGCCGCGATTTGAACCCAAACAACATC2160.1303387600922026No Hit
TTAATTGGGGGGGGAAGGGGAGGTGGGCAGTAATTAGATATCTCCCAAGAAT2160.1303387600922026No Hit
GTCATGGGGTCTTCACGTGGGCCGATCGTTTTTTTTTTTTTTTTTTTTTTTT2140.1291319197209785No Hit
ACATCGGGGACACAGTTGAGTTGCAAGCTGAATTGGCTGTGTTCAAGGCATG2080.1255113986073062No Hit
CTAAATGGGTGTGGTAAACATGATCAGCAGTCACACTCCTTGTTATTTACCT2070.12490797842169415No Hit
TCGTATGGGAGCAGGAGAGAAGGTAGTACTTTGTCCCAAGTTAGATTAGTTT2070.12490797842169415No Hit
TGTCGCGGGAGCACAGTTGCAGCATACAAAATCAATAAACAAAAATCAATGA2060.12430455823608212No Hit
TTAGCAGGGAGAACAGTTTTATAGATAAAAAGATAATCATAGTTGCTATAGT2050.12370113805047006No Hit
ACTCGGGAACCAGCAATGAGGCAGAATGATGTACCAATATTAAGCCTAATGT2020.12189087749363392No Hit
TGTTTAGGGGTTTTAAATTAGCTGCTACTAGAATGTTTTGATCAAAATGAAA2010.12128745730802187No Hit
TGTTTAGGGGGTTCTCATGCTGGCAACAAGCTGGCCATGCAGGAGTTCATGA2000.12068403712240981No Hit
CACGTAGGGATTTCAAACTTGTTGCCGTGACCAATTTTTACAAAACTGAATG2000.12068403712240981No Hit
TTTACTGGGGAATGACTTCATAGTGATTTGGAAATTTAATTTGCCAAAGCTC2000.12068403712240981No Hit
TTTGCAGGGAAATGATGACAGCAAAGTGAAGACCTTGTACAGAATGTGTTAA2000.12068403712240981No Hit
TTTATGGGGAGACTCAGTTTTTCCATCTTTCAGATGGGACTGTGTGAGGAAC1990.12008061693679778No Hit
CAAACAGGGCTGTGGAAAGCTAATGCTTCTGAAAAATTGGGTGTGCTTACAT1990.12008061693679778No Hit
GTAAAGGGGCATCAAAGAGGGGGAGCGCCTGGAGGTCCGGATGAAACGTCTG1990.12008061693679778No Hit
TTTTAGGGGGTCTCGTCCTGCCCGCACTGCCTCTCCCAGGAACTGGAAAAGC1990.12008061693679778No Hit
GTTAGTGGGGTTCTTGAGGAAAGCCGGTGGAAGATTCTGGAATGCCGTGCGG1990.12008061693679778No Hit
GCCATGGGGTAAAAATAAAACGAAAAATACCTTTCAGCAAATACAATCTTTT1990.12008061693679778No Hit
GTTTCTGGGGTGAAGGGAAATGAGGGCTTTAGTTTCCTGGGCAGGGAGGGGC1990.12008061693679778No Hit
ATCTGCAGGGCGATCTCATAGCGGGATAATACATACTTTTCCACAAAGCATG1980.11947719675118572No Hit
CGTTGCGGGGAACCAAGGCAATGCTGCTGACTTTGATATGTATGACTCAGTC1980.11947719675118572No Hit
TTGCCAGGGTTGCTGATAGTAAGTACCCTTAATCTATTAAAAGAAATACAAA1980.11947719675118572No Hit
TGCCGCGGGAAGAGCCCTCTGGCACAAATGGAAGAAGAAAGAAGGGAGCATG1970.11887377656557367No Hit
TACGTAGGATCAACAGAAATAAAAGGAAATAATAAATACATGCATAGTGATT1960.11827035637996161No Hit
ATGCGGGTATTAGTGCTGCAGAACTTCGCCATGTGATGACAAACCTTGGAGA1950.11766693619434958No Hit
AAGCTGGGTGTTGGACTTTTAAAAAACCAGTTGGAAATATAGGTTGGGTTTT1950.11766693619434958No Hit
TGCCTTGGGGACCCCGGAGGCCGGCGCGGACAAGCGGAGCCCACGGCGGGCC1950.11766693619434958No Hit
TGGTTAGGGGAGGAATCAAGGAGAAAAATCCAAATGATTTTCCTTTTTTTCT1940.11706351600873752No Hit
GATCTTGGGGTGCCTCATCGCGGCCGAGGCAGGCCTTTCCGAGGAGGAGACC1940.11706351600873752No Hit
AATCAAGGGATAGCCAGGGTCAGAAACTTGGTGACAATCACATGAGACCATG1940.11706351600873752No Hit
TAAGCAGGGAGTGATGTAGAGACCTTGGTTTGCTTTCCCTCCAGTTTCTACC1930.11646009582312547No Hit
ACGTATGGGAGCCCAGCATGCTTCACTACTCTATGTTATCTTCCTGGCCATT1920.11585667563751342No Hit
ACACCTGGGTAAACAGAACCACTACCAAGAGCTCAATGACATCCTCAACCTG1920.11585667563751342No Hit
ATACAAGGGATGAAGAGGCAGCTGATGCCATGCACTTGTCTGAGGGCATAGC1910.11525325545190138No Hit
CGTGGCGGGTAGTATTGTTTATCAGAAAGAGGAAGTGTACTTAAGATTATTT1910.11525325545190138No Hit
AATTGAGGGACTGTTTTCCATGGTGGCTGCACTAGTTTATATTCCCACCAAC1910.11525325545190138No Hit
CATGCAGGGCGATCTCTGATCGGGAGAGTGGGGAGCAACAGTTTGATTTTCT1910.11525325545190138No Hit
CACTACGGGGTGTTAAAAAGCACTAAAAGCAAATGTTCATAATTTCCATTCA1900.11464983526628934No Hit
GCATTTGGGTGGTGTAAGCTGAATATGAGATTTTTGACAAAATAAAAATTTT1890.11404641508067728No Hit
CATTCAGAGGAAATAAAACTTGCCTGATTGGACTTTCTTTCCCCTGATAGAA1890.11404641508067728No Hit
TGGATAGGGTCTGGAATTCTTTGGTGTCTAAGTGCATGGTAGTTTTTTTTTT1890.11404641508067728No Hit
TATCAAGGGCAGTCTGAATGCTGCCACAACCAGCCAGGCAGGTCCACAGAGA1890.11404641508067728No Hit
TTGTTTGGGGGGTTATATTGCAAATGATCTGTACTTTAGGGTAGAATTCAAT1880.11344299489506524No Hit
TCACCGGGGGGGGATATTCAATGACAGTTGGATGTCTTTGATTTTTTTTCCA1880.11344299489506524No Hit
CACACGGGATCGAAGTATATTGATGTATTCTAAAGCTAGTAAACTTCCCTAA1880.11344299489506524No Hit
CGAGCTGGGGGTGGAGCTGCTTATCAAAAACGAAGACATTTTATTTTAAATT1870.11283957470945317No Hit
AGAATTGGGTTCTCTCATCTAGGTTCGTGCACTTAACAAACCTATCGTGATA1870.11283957470945317No Hit
TAGTTTGGGCATTTTCCGAATATACAGCTTGGGCAATGGTAGATGGTGGTTC1870.11283957470945317No Hit
TTTTGCGGGGGAGAAGTAAAAATATGTCTTAAAAAATCATTGATGTTATAGT1870.11283957470945317No Hit
CTAAGGGAATGGACACATCAAAAGCGAGCAGGAGTAGCTGTTCTTATATCAG1860.11223615452384114No Hit
AAGTCTGGGATATAAAATTTAAAGTAGTTTTGTCTACTTCTGTAAAGAATGT1850.11163273433822908No Hit
ACTCGGGGAAGAAATACAAGAGAAACAGATCTTCAGGGAAGGGAGACAGTAT1850.11163273433822908No Hit
TTGCTGGGGGCATGCTTTTGGTTTGTTACCAAAATATACAGTGTGGTGAAGG1850.11163273433822908No Hit
CTTCTAGGGATTCACTTTACAAATATGTATTGAATTCACACTATGCTGAAGT1850.11163273433822908No Hit
ACTAATGGGGATATTCAGACCTTGACTTTGAGAAGGTCATGCATACAAGGAA1850.11163273433822908No Hit
ATGCAGGGAATAACATTTTTAAATGAAAATTAACTATTGAAAACAAAAATTT1850.11163273433822908No Hit
TTGATCGGGGGTTAGCCTGAAGTCTGAATGGAGCCCATAGTTGGAAAACAAC1850.11163273433822908No Hit
CTGTATGGGGAAAGCTAACCGTCTAGAACCAAAGCTTACAAAAATCTCTCAC1840.11102931415261703No Hit
CGAAATGGGACTTCTATTTATCATTGTCCTGTAGAAAAATAAGCAAATGACA1840.11102931415261703No Hit
CTTGTGGTGGGAAATGAGAAGACTGTAGCTACTTAGAGTGTATGCTTGTAAT1840.11102931415261703No Hit
TAGTCTGGGCCACAGACATCTGTATCATCAAGCTTTCCCTACACCAAAGTCG1840.11102931415261703No Hit
ATGACTGGGAGCTCTGCTTAGGGACAGACTGCCTCCTCAAGTGAGTTGAGGA1830.11042589396700497No Hit
GCTACTGGGGCTGAAGAAACATGAGCATCTGCTTTAAAATTAATAGTTAATT1830.11042589396700497No Hit
TATTCTGGGGTGGTAGACACTCTTGAGCTGGACTTAGATTTTATTCTTCCTT1830.11042589396700497No Hit
GTACCGGGGGCGTGGGGAGCCTTGTCGGAGAGAGCTGTGGCAGTGGTTGTGG1820.10982247378139294No Hit
TTTGGTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1820.10982247378139294No Hit
CTAGGCCGGGATGTATAAAGTGAAAGATAGTTTACTTATCTGACTTTGATAT1810.10921905359578088No Hit
GTAGCCGGGCAGCGGCCTCCTTACGGTTACTACTGATAGAAATGTTGGCAGC1810.10921905359578088No Hit
GTTCCAGGGGGTTTTATTTACATTTTAGGGTGGGTAAGAAAGCCACCTTGTT1800.10861563341016883No Hit
GTTAGAGGGAGAGGAGCTGTCCGTGTGCTGAAACGGCCCGAGAAGCTCGCCC1800.10861563341016883No Hit
GTCTGGGGAATGGTTCTAGATGTCCCAAGAAATCCTAGACCCCTTGTATCCA1790.10801221322455677No Hit
AAATAAGGGGCTTTTCCGCCCGCTCCCCCCTCCCCCCGAGCGCCGCTCCGGC1790.10801221322455677No Hit
CTGCGGGGGTACCATGGACGGAAGCTATCGGTGCAGGGCTTCAAGGAGGCAC1790.10801221322455677No Hit
CGAAAAGGGGGGCTGAATGCAGCCATCTGAGCAAGGGCTGAGGGAAGCCATC1790.10801221322455677No Hit
CTCTGTGGGATGAAAATAATCAGCAGTAAATTGAACCTGCATAAAAAATTCT1790.10801221322455677No Hit
GTTCTAAGGGCAGTGGCCTGATCTCGGCTCACTGCAACCTTCTCCTCCCAGG1780.10740879303894474No Hit
TACAAGGGGAAACTTTTTTATCTTGAAAAACAAAATTGAAGGAAATACTTTT1780.10740879303894474No Hit
CAATTCGGGAAGGATTTCAAGGAGCAGGTGATACACCACTTCGTGGCGGTCA1780.10740879303894474No Hit
TAGCATGGGGGTAGTAAAATCCTTAATGAAACCAGTGGATGTGCTTAACGTA1780.10740879303894474No Hit
GCAATAGGGAAGAGTGGCAGAGGCTACTACAAAAAGCAACCTTTTCATTTTC1780.10740879303894474No Hit
GAAACGGGGAAGCTTATTTCAGAACAAGTCTAATATTTCAGATTCTTTCTTT1780.10740879303894474No Hit
TCTGCTAGGGCGATCTCATAATGGGGCTCACCAACACATCTTTTTTATGATA1770.1068053728533327No Hit
TCAGTAGGGATGCAAATAAATACTTTGCCATAAAAATAAGAATATGCACAAA1770.1068053728533327No Hit
ATAGGCGGGAGCTCGACGAATCCCTCCAGGTCGCTGAGAGGTTGACCAGGAA1770.1068053728533327No Hit
CTCTCTTCGGGAAGGCTGAAGGGATTGGTGTCAATCAAAGACAAATCACAAC1760.10620195266772064No Hit
GGAATAGGGAAGAATGAAGCTGGAACTTTAAATGGTTTATGTGTGTTTTATA1760.10620195266772064No Hit
AATGCAGGGAGGGTCAGCACTGGGTCAGCGACTCCAATCTTCCAGTGGCCAG1760.10620195266772064No Hit
AACACTGGAGTCTCATAATTTGGAATTTAAGATAATAAGTAGTAACAAGGCA1750.1055985324821086No Hit
GGCATAGGGGCCAAATCTCTCACCAGATAAGTCCTTTTCTCCCTGTGCCGTT1750.1055985324821086No Hit
AACTGAGGGATTGAATATTTGTACTGATAAAAATGCAACCCAATCCATACCT1750.1055985324821086No Hit
TCAGTCGGGTCCTTCCGTGACTGGTCACGTTGTCTGCCTGGGCTCAGCGTGG1740.10499511229649654No Hit
GCCAATGGGACGGAATCTACCATTTTAAAGTTAATTGCTTGTCAAGCTATAA1740.10499511229649654No Hit
CAGACAGGGATGATAAAGCATTATCACTTGCTTCTAGAAAAACTTAGTGGTT1740.10499511229649654No Hit
AGCGCTGGGCAGGGTCTTTGCTATACAAAAGTGTTTTCCACTAATTTTGCAT1740.10499511229649654No Hit
GCATTAGGGGACCTGAGAAAACGAGCCTGTTTCAGAGGAACATCGTCACAAC1740.10499511229649654No Hit
AAATCAGGGGATCACCAAGACAGAAAGTCAACAAAGAAACAATGGACTTAAA1730.1043916921108845No Hit
CTGTAAGGGGGGAGCCGCGCCGGCACAGCCGAAGGGAGCGGGCGAGCGGCGA1730.1043916921108845No Hit
CTGCCGGGGATTCTGAATAATGGAATGGTAGTCAAATTGGCAGTAGGCTTTG1720.10378827192527244No Hit
GGGCTTGGGTACCTCAGGCCTGATCCATCGTGCCCTTGACTTTGCCGTCTCA1720.10378827192527244No Hit
GCAGCAGGGCATACAAAATTTATTACTGTTTGCCATATTTATAGCAAACATA1710.1031848517396604No Hit
AAAAATGGGGTCTTATTAATCACCAGAAAATGGTCATATCAAATGTTCTTTC1700.10258143155404834No Hit
GAAGTCGGGGATGAATTACTAATGCCTTATTTTCTAGGCATATAATAATAGT1700.10258143155404834No Hit
GGGTTCGGGTAATCTGAACTTCAGACTGTAGTCTCATCCAAAAATGTTAACA1700.10258143155404834No Hit
CAGTCTGGGGGGGGAAGCGGAGCCAACATGCCAGTGGCCCGGAGCTGGGTTT1700.10258143155404834No Hit
GCTGCAGGGTCCAGATGAAAAAAATAGAGTGCGCAACTTCCTTTTAAGGCAT1700.10258143155404834No Hit
TTCTTGGGGTACATAGACAATATTAAATTTCTTCATACTAGAATAATAAAAG1690.1019780113684363No Hit
GTTAGCGGGGCTAAATGATTTCCACAATAAAGTTTGTTAACATAGCCATCAT1690.1019780113684363No Hit
CGATTAGGGAATCCTTTACTTTGAGGCTTTTTGTCTGCTAAAAATGTTGACG1690.1019780113684363No Hit
ACAACAGGGGGTCTGACTCATCACATGTAAACGTACTGCAGCTTTGCTATTA1690.1019780113684363No Hit
TATTATGGGCCTGTCCACCTGTCACCAGCACAAGAGAATTAGAGCTTCAGTG1690.1019780113684363No Hit
CTTCATGGGAGAGCCAAGTTTAACGAGGTTTTCCTTTGACACCCTGAGAGCC1690.1019780113684363No Hit
AAACAAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAAACCACAAA1690.1019780113684363No Hit
TTTGGCGGGATTCCTCCAAGTGGAAATTTGGACATTACCCTTTGTTCATGGT1690.1019780113684363No Hit
CTGTCTGGGACCGAGCTGTGGAGTTCCTGGCCTCCAACGAATCCCGGATCCA1690.1019780113684363No Hit
TGTCAGGGGATATTATCAGCGGTTATTCGGTGAGCGGTGGTGGTTTATTCTT1690.1019780113684363No Hit
GTCCTTGGGAAGCCTTAAGATATAGGTATTATTTTCTCCCTTTTACAGCTGA1690.1019780113684363No Hit
TGCTCCGGGCTGAAAAATATGTATTATGCATTCTTGGCCCATCTTTCTGTTG1680.10137459118282424No Hit
TAACACGGGGAAAGCAAAGCAGGAGCAGGCACATCACATGGCAAGAGAGCAA1680.10137459118282424No Hit
CCGTGACGGGGAAAGGAGATTATTTTAGGTATCTTTCTGAAGTGGCATCTGG1680.10137459118282424No Hit
CTGTGAGCGGGACCGCCTTGCCCAGAAGAAGCCCTTCACTCCGGTCAAGTAC1680.10137459118282424No Hit
ATGCAGGGTATGCAAAATGTTTTCCTCCCCAATGTAGAGATATAAGCCAGTT1680.10137459118282424No Hit
GTCACCGGGATTTGTCTAATTGCAAGGCAAGTCAAGCTCTAAAACAATTGCT1670.1007711709972122No Hit
ATAGCTGGGGCCAATGCTTAAAAATTAGAAGAATATTCACAAATACTCAGAT1670.1007711709972122No Hit
GTAATAGGGCATCCCTGAAAACGCATTTATCTTCTCAGATACTTATATACTA1670.1007711709972122No Hit
ATAATAGGGTGCATGCAGAGAGAATGTCTTCATAGAGAGAATGTCATTAAAT1670.1007711709972122No Hit
CTAGTCGGGGACTCAAGACAATTTAAGAAATAGTTTATTTTTACTTTCACAT1670.1007711709972122No Hit
CGTAGCGGGTCCAATGCAACTCTAACTGCATACTTCTGAAATGTTTACCCCC1670.1007711709972122No Hit
GACTAAGGGGTAAAGTTCCATTTCTGACAGCTGACATTTATTAAACTTTGGA1660.10016775081160013No Hit
GTTGGGGGGGAAACTTCTTATTCAGGTTTGAGTAAGCATTTTACTTCCATGT1660.10016775081160013No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)