FastQCFastQC Report
Sat 31 Dec 2022
SRR5845318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25150
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT2160.8588469184890656Illumina PCR Primer Index 5 (95% over 21bp)
ATCCTAGGGAAAGAGAATTCAAAAAGGGCTGATGGTAGGCTTTGAACATGGG1920.7634194831013917No Hit
CCGCATGATGAAACTTCGGCTCACTCCTTGGCGCCTGCCTGATCCTCCAAAT1860.7395626242544732No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC1730.6878727634194831No Hit
AACTTTGGGAGAACCTGCATTTAAGAACAGAACAGCAAGTATGAACCACATG1650.6560636182902585No Hit
GCTCAAGGGATGGGTGAGTTTTTTTCCTTGACCTACTTAGAAAGGTATCTTT1570.6242544731610339No Hit
TTTCTTGGGAGGCCCCAACGTTGTAGGCCCCTACGGGCTACTACAACCCTTC1550.6163021868787276No Hit
AGTTTTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1510.6003976143141153No Hit
CTATCTGCGGGATTCTGATAAAATCTCTTGAAAATACTGAAATCAAAAGGTT1480.588469184890656No Hit
TTGTAGGGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATA1440.5725646123260437No Hit
GACCTAGGGCGAAAAATTCTAGGCTATATACAACTACGCAAAGGCCCCAACG1430.5685884691848907No Hit
TCTATAGGGATGGCTACCTTAAACAACATCAGAAAATTCATACTGGCATGAA1410.5606361829025845No Hit
TACGGCGGGTCCCTATTGTTGGCTCCACAGCCTTAAAGTGCTATAGATTTAA1400.5566600397614314No Hit
TTCACAGGGGGACAAGATCGACGTGATCAAGCAGGCTGACTATGTGCCGAGC1340.5328031809145128No Hit
TTCGCTGGGGGAGGAGGAGGGACTCGGCGGGAGGATGGGCTTGCTCTCAATT1330.5288270377733598No Hit
CTCATCGGGGTTGGTTTGCCTTGCAAAACAGAAAAATGGAATTTTAGCTCAA1310.5208747514910537No Hit
ACTGCTGGGAAAGGAATTTTAACAAAGAGCAATCAGTATTATTGGACCAAAT1300.5168986083499005No Hit
CTAGCCAGGGGATGGCTGAGGGCAACCCGCCCACGGAACTGACGCAGTCGCA1260.5009940357852882No Hit
GTAAATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1220.485089463220676No Hit
CTTTAGCCGGGCCATCGCTGGGTCAATAGTACTTGCCGCAGTACTCTTAAAA1210.4811133200795229No Hit
GTTGTTAGGGGAGGACGTCCAACTTCTCCCTGATGACGGAGCTGAGCATCAA1190.47316103379721675No Hit
TCAAACGGGCATAATGCTTCGTGACGGCCACCAGGACAGAACCACCTGATGT1170.4652087475149105No Hit
GGCAACGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1160.4612326043737574No Hit
GTGTAAGGGAGGCAGAGAAAACATCAGGGAAAAGGAAGGTCCTGACTCCCTG1160.4612326043737574No Hit
TATAAAGGGGCTCATAAGTAAATGTCTTCATGTTGATGTGTCCGTGAAAGTT1130.4493041749502982No Hit
GACAGCGGGCTCCCTCTTAGATTCTTGTACCAAGCTTCTTCAGAAAGGGATT1090.43339960238568587No Hit
ACATAAGGGGGAGGGCGGGGCCGGGCCCGGGAGCCTGTGGCTTCAGGAAGAG1080.4294234592445328No Hit
ATAAAAGGGAGATCTATTAATGTAGGGAATTTTTCCCTAAGATTGAATTCCT1070.4254473161033797No Hit
CTTGCCGGGTCTTCCGATCTCTATATGGGGTCATACATTCTTCTTGCTGTTG1060.42147117296222664No Hit
CTGGACGGGACTTACAAGCTGCAGATAGAATAAAACTATTCTTTATGTTTTT1060.42147117296222664No Hit
ATCCAGGGGTCCGATCTCTAGAAGGGATTTGCAGGCTTTTCTGAATACCAAA1050.41749502982107356No Hit
ATATAAGGGATTTCCCTCAGTACTGTAAGCAAAAGTGATTACGGGCAGGGAC1040.4135188866799205No Hit
CCAACAGGGATGAGCTCTGAGTCCATTAAAATACAGAATGAACATTTAAGAA1030.4095427435387674No Hit
GACCTTGGGGCCCGCCACCATGCCCGACTAATTTTTTTTGTATTTTTAGTAG1020.40556660039761433No Hit
CTTATCGGGGGGCTGACAGAGCAGGTTCTCATTTCTCAGCTCTTTCCCTTAT1020.40556660039761433No Hit
ACTTACGGGACTGATTTCCCACCGGGGACTGGACACAAACAGGAATGTCTGG990.39363817097415504No Hit
AAGATAGGGAGAACAGAAAGTGAGGGGCTCTAAGCAGATGAATACACTCCAG990.39363817097415504No Hit
CAATTTGGGCTGGGGGTCAGAGCGAAGAAGAGGCATCATTCAACCTCAATGC980.389662027833002No Hit
ACTAGAGGGGAGCTCAAGCCCGCAGCTCGCAGGGAGATCCAGCTCCGTCCTG980.389662027833002No Hit
CCTAAAGGGGTGATCTCTCGGTGGGGGGCCCATCTCTGCTGACCTGGGTGTG960.38170974155069587No Hit
AATCATGGGGGCATACGTATTGTTAAATGATAAGGTTTAGAATGACAGGAAC890.3538767395626243No Hit
GCTATAGGGGGAGGGAACATTCTGCAGTATAAAGGGAGCAGGGAAGGCGGGA860.341948310139165No Hit
AACGGGGGGAATGCAAGTTGATGAAAAAATTGCTAGAGGCCCACGAGGAGCA860.341948310139165No Hit
TTTCTGGGGATGGTCCCACTTCCCCATCTGTGTGTGAATTCCTTTAGACTAA850.3379721669980119No Hit
ATCCACGGGATCTGTCTGGTAGCAGTGAGACCCCTTGTCTTGGTGATCCTTA840.33399602385685884No Hit
GCACCCGGGAACTTGAATGGCCTTAAACCTGTTTCAGCTTTAACAGTAGAAT840.33399602385685884No Hit
CACCTAGGGACACAAAAAGAAAATACATATGCAGTCTAGTCAGACAAAATAA820.3260437375745527No Hit
ATTATGGGGAGATAGAAGCGATGAAGATAAAGAAAAAGCAAGTTCTTTGATC810.3220675944333996No Hit
ATCGCAGGGCGTAGAAGAGAACTTCATATTCGAACATATGCAGTTATTCCAC800.31809145129224653No Hit
AGCATAGGGACTACACTGTCTTATATACTCGTAACTTCCCCGTAGGTCTTGA800.31809145129224653No Hit
AACAAAGGGGAGGAAGGCGCCGGCCTTGGAGGCGCCACGTCCCTTGCGGCGG790.31411530815109345No Hit
TCTCCTGGGCAGAAAAAAAATGCAAGTGACTAAATTCAATTATTGACTAATT790.31411530815109345No Hit
TAGCCGGGTATTTTAAAATTTATAAACAAGTCACTTAAATGCCAGTTGTCTG790.31411530815109345No Hit
GTTCGTGGGCTACGACCACATCAACGAGGGGAAGCTGTGGAAACACATCAAG780.3101391650099404No Hit
TATATTGGGGACAGACTAGAAGTTAAGTTTAATACAGAAAATGTCCTGTTCA770.30616302186878724No Hit
TGGAAGGGGATGCATTACTCAGGTTGGTGGGGTCGGTTTGAGAAGATATAGA760.30218687872763417No Hit
TCTCGTGGGGATCTATGTAAGGGATGATAAAAAAATAAAAACAGCCCAACCG760.30218687872763417No Hit
TCATACGGGAAACTTTGTGAGCTACGTGCAACTGACGTCGCGGGCGCTGCCC750.2982107355864811No Hit
CCAAGGGGCAGCTTTTCTTGTTGAGCAGAACTATCATCTGCTTTCCCAGATC750.2982107355864811No Hit
TTGGCCGGGAGGGGGTGGGAAGGTAGGATGTGGAGTGACATGGTTCTATCCT750.2982107355864811No Hit
TCAGTTGGGCGATCTGATTTGGGGCTAGCACAAAAATTGGGGGATGTTAAAA740.294234592445328No Hit
CGGCAAGGGGTTGGCCAGGATGGTCCTGATCTCCTGACCTTGTGATCCGCCT730.29025844930417494No Hit
AATTTTGGGCAAGTGTTTTAGGATTAGCCTAAAATTGGACATTGTCATTGAT730.29025844930417494No Hit
GGCCAAGGGAATGAGAAGAAGCACAATTTTGATTGTGATGAGTCACTTTCTG730.29025844930417494No Hit
TCTGGAGGGCCTTCCTCATATGAATAACCATTTATATGTGTTTTGCTTAAAG730.29025844930417494No Hit
TCTGATGGGAGGAGGTGGCGGCGGTGGCGCTGGGAGCTCCTGTCACCGCTGG720.28628230616302186No Hit
ATCAACGGGACTGCCCAAGGACCAGTATTTGTCACTACCAAACTCAGCCCTT710.2823061630218688No Hit
TCTGCGAGGGATGCACACATATATTTTCCCGCATAACCGTATCCAATGCTAG710.2823061630218688No Hit
GAAATGGGGAGCATTGTGAGCGTAACAGCCTACATTGCCTTGGCCCTGCTCT700.2783300198807157No Hit
GTAACCGGGGAGTGGTCAAGGCCGCGCTCGCGGCGAGGGGCTGCGAGAGTGA700.2783300198807157No Hit
GTTGCAGGGTGAGGGAGACTGATCGACCCTATCTCAAATCCACTGACGTAGC690.27435387673956263No Hit
ACCAAGGGGATTTTTGTTGTTAAAGATTATTTTTAAATAGTAGTAATGAAGT680.2703777335984095No Hit
TCATTAGGGTCAAAAGTTTTGCTTTAGAAATAACTACATAGCCTAGAATTTT670.2664015904572564No Hit
CACTTCGGGATCATCTTGAACATTAATCCACATGTTTCAGAGCTCACCAGGC670.2664015904572564No Hit
ATTCATGGGTCTATGTTAAATTTTTTTCTGCAGAAGAAAATAATCATATTAC660.26242544731610334No Hit
ACGAGGGGGAAGTATTCATAGAGGTAGACTGTATGATAAATAAAAACAAATT660.26242544731610334No Hit
TAGAAAGGGGAACCAGACTTGCTAAGCCAGTTCTATGCTTGGGCTTAATGTG660.26242544731610334No Hit
ACTTCAGGGTGAACACCCGCTGATCCTTTAACAAGGATTTCTGGCAGGAAAC650.25844930417495027No Hit
TGTTTTGGATGAATGTGCAAGAGGCCTGTGACCGAATGCTACGTTTTTATGG650.25844930417495027No Hit
CTTGTAGGGGCCTCTTTCCGTCTCAGGTCGCCGCTGCGGAGGGAGCCGCCGC610.242544731610338No Hit
TCGTCAGGGTTGTGACTAATTGTATAACAGGTTATTTTAGTTTCTGTTCTGT610.242544731610338No Hit
TACCAGGGGTATACGCGGGATGCACAAGACAATCAAATATAAATCTGTCCTC610.242544731610338No Hit
GCCCCGGGCGCAGCCTGACCGGGACCCCTCCCTCCCACAGCCCAGCCCACCT600.2385685884691849No Hit
TACTATGGGAAGATTTAGGCATAATCTGGTTTTAAAACCTTGATTGAAACTG600.2385685884691849No Hit
GCTGAAGGGAGGTGTTATTTATTAACGTATTATAAAATAATGTTTGATCCAG600.2385685884691849No Hit
AGGTTAGGGATTCATTAAATATTTAATAAAATTTACCAGTACATTTACATAA590.23459244532803178No Hit
GCCAGCGGAAATGAATCTGAGGAGGCTACTCAGTAGACAGTCCCACCCTCAC590.23459244532803178No Hit
TTGGTACGGGGCAGACTTGAGGTGATGATAGTTGGCCTCTGGTCTACAAAGA580.2306163021868787No Hit
GAAAAAGGGTGGTGCAAACAAACTCAAGTGCAAAGGAAACGCGACGAAGAAC580.2306163021868787No Hit
TTCCTTTGGGGAGCCCTGGCAGCTGTCTCCAGAGGATCAAAGCCACACCCAA570.22664015904572563No Hit
GATGGCGGGGTTGTTCTTAAATCAAAAATTGGATAATTTTTAATATTTATGT560.22266401590457255No Hit
CGTAACGGGAGCTTAAAACTAGTCAAGCAGTTTAGAACCAAAGGCCTATATT530.21073558648111332No Hit
CAGACTGGGAAACTTTGTGAGAGGTCTTATATTTGAATGGACCCTTAATGAT530.21073558648111332No Hit
GAAAACGGGAGGTTGGGGGTTGCGTCGCTCTCTGGTAAAGGCGTGCAGGTGT530.21073558648111332No Hit
TCGCCAGGGCGATCTCATCAAGGGTGTTGGAAGTGACCCAGCCCCCTCCCCT520.20675944333996024No Hit
GTGATGGGGGATTCCAGCAAAGTCTCTAACTGCAGCCTGTAGACAATTTGCT510.20278330019880716No Hit
GTTCAAGGGAGCTTGACTGCTTTTAAATGACCAAAGATGACCTGTGGTAAGC510.20278330019880716No Hit
AAGCCCGGGTCCATTTTAATTTCCTCAGTGGTTTTATGAGAAGTTTTATGGG510.20278330019880716No Hit
ACTACGGGAGATTCACACTTTCTGGCCTAAACCCTAATGGGATGAGGCTTTT480.19085487077534793No Hit
TAATAGGGGGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCA480.19085487077534793No Hit
CGACTGGGGGAGGAGTCCGAGGTGGGGTTGGTTACCTGTCCCGAGGGGACCC480.19085487077534793No Hit
GCAGTAGGGGTGGTGAAAAACGCGGGGATCCAAAGAGCTGGATTTTTATCTC480.19085487077534793No Hit
TTTGGCGGGGATCTGGAGAGCGGCGGGCTGCTGCATGAGATTTTCACGTCGC470.18687872763419483No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTG470.18687872763419483No Hit
TATCCGGGGACCCTATTCTATCATTTGCTGTTGCTTGCTGAACTGTGAAGAA460.18290258449304175No Hit
TCCGTAGGGACTGAAAATACAAAAAATTAGCCAGGCGTGGTGGCGGGCACCT460.18290258449304175No Hit
GCGCTTGGGGGTGTTCACATATCCCTTTCTCTAACAGTTTAACCTAGACAAA460.18290258449304175No Hit
CGTCCGGGAAGACATGAAAACAAGACTGGGTAGTTGTAGATTTCTGCTTTTT450.17892644135188868No Hit
GTCGCACGGGATTTGACCGGGACCTAGTGGTCACTCAGTGTGGCAATGCTAT450.17892644135188868No Hit
TATTCTGGGGTGTATGGTTTGCAGCTTTTAAAAACCATTTTTTTAAAACTAA450.17892644135188868No Hit
GTTACTGGGTGCTAAGGGATACAGGGTTTCTTTTGGGGGGATGCGAATGTTC450.17892644135188868No Hit
CATCGAGGGATTTAAAATTGGAATGTGAGACATGTTGCTGTGACCTGTTTTT440.1749502982107356No Hit
GGCCATGGGAGAATATGCTTTCTTGGACCATGTTTGAGACTCAGAAGAATGG430.1709741550695825No Hit
AAGCATGGGGACAAGGAACTTATTGATCATTCTCCGAAAGCAACTCCTGACA430.1709741550695825No Hit
TCAAAAGGGGCTGCTTGTACACTCATTCATTACTAAAAGTTTTGAGAAATTT430.1709741550695825No Hit
ATTTGCGGGATTGAAAAGTTGGGAAATGCCTCACGCCATAAATACTAATTGA430.1709741550695825No Hit
GATTATGGGGGGAGGCCAAGTCTATGATACTGCCTCAAAGAGACCCTATTGT430.1709741550695825No Hit
GTGTATGGGGTATGGTCATATTAGTAGCTATTTGTTGCAAGATACTTGAAGC420.16699801192842942No Hit
TTACAAGGGCAACTGCATATTGAAATTTGTTAAAGCACTCATCTTTATGGAA420.16699801192842942No Hit
ATCGCGGGATGCCGGGAAGGGGCATTATTTGTGCACTGAGAACACCGCGCAG410.16302186878727634No Hit
TTGAACGGGTTCAGTAATGACTGAGAATTGAATACAAATAGATTACAAAGCC410.16302186878727634No Hit
ATGCATGGGGCAAATTATGACTGCGTGAGCCTTAGAAAATAAAATGTATAAA410.16302186878727634No Hit
TTCGCCGGGCGCAGGAAGTCCCTCAAGCAGAAGAAGAAGCGGTAATGCGCCC410.16302186878727634No Hit
GCCCTGGTGGACAATTTCTTCACGGGCAGGAAGAGAAACGTGGAGCACTGGA400.15904572564612326No Hit
CACAGCGGGAGATAACTTTTGTAGCAGTGGTTATATTGCTTATAATTTAATG400.15904572564612326No Hit
GAAATGGGGTTAGATTCTTTAGGGTATAGCAAATGTGTTCTTATTGGCCATG400.15904572564612326No Hit
CAACTTGGGACGCGGGCGGCGCAGTCATTCTCATAATCGCCCACGGGCTTAC400.15904572564612326No Hit
TATCGGGTCTGAAGGTGGGTCTAGCTACGGGGTTAGGACCTTCCTGTAAACT390.1550695825049702No Hit
GAATCCGGGAGTCGCGGACGCAATGGCTTCAAGGTTACTTCGCGGAGCTGGA390.1550695825049702No Hit
AGCACAGGGGGAATATTTTAGATTCTTGGGTATCTGATAATCTGGAATTTCA390.1550695825049702No Hit
GTTAGCCGGGTATTTTGGTAATGCTTATCTTCAGTTTAAACATTTATTCTTT370.147117296222664No Hit
CTCCGTGGGATATCTGTAAAGATAATCGAAGCAAAAGTAATCACTTAAAGGC370.147117296222664No Hit
ACAGGGGGAAACTATGTAAACTTCATTTTTCATGCAGTGCCCAACTACTGCT350.13916500994035785No Hit
TATTTAGGGGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTAG350.13916500994035785No Hit
TAAATGGGGGGCATTGCCGTGGCAACTGAACGATCCTAACTACTTCAGTATT350.13916500994035785No Hit
CATTGCGGGGTAGAAAGCATGGAAGCAATTCCATGCATCCGCAGATGGGCAC330.13121272365805167No Hit
TTGCTTGGGGACGCTCTTCCGATCTGACATTGGGAGAATGAAGAGCAGCAGT330.13121272365805167No Hit
TAGCACAGGGATTAGGACAAATGTGACAGTCAAAAAAAGGATTAGAGGGAGG320.1272365805168986No Hit
ATCGAGGGGGTGTTTTGTAGACTAGTGTTCTTCCATCCTTCAACTGAGCTCA310.12326043737574553No Hit
ATAGCTGGGGAGGCTTGTTCCTCGACTTCCCACTCTTCCCATATCTGGTTAA310.12326043737574553No Hit
TCTCGAGGGAATTGAAGTCTGCTTCTAAAAAAGTACAAAGTCATACTCATTA310.12326043737574553No Hit
GCTAAAGGGAAGAGATACTTACAGATGTTGAGATGGCTGCCCTGCATTTCCA310.12326043737574553No Hit
GTTGCAGGGATGAGTCTTAAACCAAATATACTAACTTGGGCCATGGGGCAGA300.11928429423459246No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGA300.11928429423459246No Hit
AATATTGGGCGGGTACTCACACACGCCCCCCAGCCCAGCCATACGACAGCAG300.11928429423459246No Hit
CTCATAGGGTAACCAAGGAGGTGAAAGACCTCTACAAGGAAAACTACAAAAC300.11928429423459246No Hit
TCGTGCGGGGCTGAGAACGCGGGTCCACGCGTGTGATCGTCCGTGCGTCTAG300.11928429423459246No Hit
TGATAAGGGACTACAATGATACTATCATTTAATAATATTAATATTACTTGAA300.11928429423459246No Hit
TAGATGGGGCTTTGCAGCCTCCTCCATGAATCCCAATGGCCTGCACTTGTAC290.11530815109343935No Hit
CAAAAAGGGTGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTC290.11530815109343935No Hit
CTATGTGGGCATAAGCAAGATGGTAAAAATCTGGAAATTGAACTTAGCTCAT290.11530815109343935No Hit
GTTTCTGGAGGGCTCTGTAGCTTCTCAGTATAAAGGCTCCTTTCATGTCTAA280.11133200795228627No Hit
AGATAGGGATGATAAATGAATCATTGCCTCACTTCGAAGATTTCTGATGAAC280.11133200795228627No Hit
GGACATGGGACAAGATTTCTTCTAATAAGTGGTTTATTTAAACTCAATTTCG280.11133200795228627No Hit
GTGGCGGGGCAAAGTTAATATGATTAACCACCTGCCGCTGATCAGCCTTATG270.1073558648111332No Hit
GTGCATGGGTATTCTGCAGAAAATTAATTGAAATACCTATTCAAGAAGATAG270.1073558648111332No Hit
GCCGATGGGGCCACTTGGACACCATGCTTAGCAAACAGAGCAAAGCAGATAT260.10337972166998012No Hit
TTATAGGGATTTGCTGACTTCTTTATAATGAATCTGATCCTCTGGGGAGAAG260.10337972166998012No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)