FastQCFastQC Report
Sat 31 Dec 2022
SRR5845317.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845317.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25658
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT2160.8418427001325123Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC1990.7755865616961571No Hit
GCTCAAGGGATGGGTGAGTTTTTTTCCTTGACCTACTTAGAAAGGTATCTTT1920.7483046223400109No Hit
CCGCATGATGAAACTTCGGCTCACTCCTTGGCGCCTGCCTGATCCTCCAAAT1820.709330423259802No Hit
GACCTAGGGCGAAAAATTCTAGGCTATATACAACTACGCAAAGGCCCCAACG1750.6820484839036558No Hit
TTTCTTGGGAGGCCCCAACGTTGTAGGCCCCTACGGGCTACTACAACCCTTC1690.6586639644555304No Hit
CTTTAGCCGGGCCATCGCTGGGTCAATAGTACTTGCCGCAGTACTCTTAAAA1680.6547665445475095No Hit
ATCCTAGGGAAAGAGAATTCAAAAAGGGCTGATGGTAGGCTTTGAACATGGG1600.6235871852833424No Hit
ACTGCTGGGAAAGGAATTTTAACAAAGAGCAATCAGTATTATTGGACCAAAT1560.6079975056512589No Hit
AACTTTGGGAGAACCTGCATTTAAGAACAGAACAGCAAGTATGAACCACATG1500.5846129862031335No Hit
TTCGCTGGGGGAGGAGGAGGGACTCGGCGGGAGGATGGGCTTGCTCTCAATT1460.5690233065710499No Hit
GTAAATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1450.5651258866630291No Hit
TATAAAGGGGCTCATAAGTAAATGTCTTCATGTTGATGTGTCCGTGAAAGTT1350.5261516875828202No Hit
TCTATAGGGATGGCTACCTTAAACAACATCAGAAAATTCATACTGGCATGAA1350.5261516875828202No Hit
CTCATCGGGGTTGGTTTGCCTTGCAAAACAGAAAAATGGAATTTTAGCTCAA1330.5183568477667784No Hit
CTATCTGCGGGATTCTGATAAAATCTCTTGAAAATACTGAAATCAAAAGGTT1330.5183568477667784No Hit
AGTTTTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1310.5105620079507366No Hit
TCAAACGGGCATAATGCTTCGTGACGGCCACCAGGACAGAACCACCTGATGT1310.5105620079507366No Hit
TACGGCGGGTCCCTATTGTTGGCTCCACAGCCTTAAAGTGCTATAGATTTAA1290.5027671681346948No Hit
AGCATAGGGACTACACTGTCTTATATACTCGTAACTTCCCCGTAGGTCTTGA1290.5027671681346948No Hit
ACATAAGGGGGAGGGCGGGGCCGGGCCCGGGAGCCTGTGGCTTCAGGAAGAG1280.49886974822667396No Hit
CTGGACGGGACTTACAAGCTGCAGATAGAATAAAACTATTCTTTATGTTTTT1270.494972328318653No Hit
GGCAACGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1220.4754852287785486No Hit
GACCTTGGGGCCCGCCACCATGCCCGACTAATTTTTTTTGTATTTTTAGTAG1220.4754852287785486No Hit
CAATTTGGGCTGGGGGTCAGAGCGAAGAAGAGGCATCATTCAACCTCAATGC1220.4754852287785486No Hit
CTAGCCAGGGGATGGCTGAGGGCAACCCGCCCACGGAACTGACGCAGTCGCA1180.459895549146465No Hit
ATCCAGGGGTCCGATCTCTAGAAGGGATTTGCAGGCTTTTCTGAATACCAAA1170.4559981292384442No Hit
GTGTAAGGGAGGCAGAGAAAACATCAGGGAAAAGGAAGGTCCTGACTCCCTG1170.4559981292384442No Hit
TTGTAGGGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATA1160.4521007093304233No Hit
CTTATCGGGGGGCTGACAGAGCAGGTTCTCATTTCTCAGCTCTTTCCCTTAT1160.4521007093304233No Hit
CCTAAAGGGGTGATCTCTCGGTGGGGGGCCCATCTCTGCTGACCTGGGTGTG1160.4521007093304233No Hit
TATATTGGGGACAGACTAGAAGTTAAGTTTAATACAGAAAATGTCCTGTTCA1130.4404084496063606No Hit
ACTAGAGGGGAGCTCAAGCCCGCAGCTCGCAGGGAGATCCAGCTCCGTCCTG1110.43261360979031876No Hit
TTGGCCGGGAGGGGGTGGGAAGGTAGGATGTGGAGTGACATGGTTCTATCCT1110.43261360979031876No Hit
TTCACAGGGGGACAAGATCGACGTGATCAAGCAGGCTGACTATGTGCCGAGC1100.42871618988229787No Hit
AAGATAGGGAGAACAGAAAGTGAGGGGCTCTAAGCAGATGAATACACTCCAG1050.40922909034219346No Hit
GTTGTTAGGGGAGGACGTCCAACTTCTCCCTGATGACGGAGCTGAGCATCAA1040.4053316704341726No Hit
GGCCAAGGGAATGAGAAGAAGCACAATTTTGATTGTGATGAGTCACTTTCTG1030.4014342505261517No Hit
CTTGCCGGGTCTTCCGATCTCTATATGGGGTCATACATTCTTCTTGCTGTTG1030.4014342505261517No Hit
GACAGCGGGCTCCCTCTTAGATTCTTGTACCAAGCTTCTTCAGAAAGGGATT1030.4014342505261517No Hit
CCAACAGGGATGAGCTCTGAGTCCATTAAAATACAGAATGAACATTTAAGAA1000.38974199080208904No Hit
ATAAAAGGGAGATCTATTAATGTAGGGAATTTTTCCCTAAGATTGAATTCCT1000.38974199080208904No Hit
GCTATAGGGGGAGGGAACATTCTGCAGTATAAAGGGAGCAGGGAAGGCGGGA960.37415231117000547No Hit
ATATAAGGGATTTCCCTCAGTACTGTAAGCAAAAGTGATTACGGGCAGGGAC960.37415231117000547No Hit
AACGGGGGGAATGCAAGTTGATGAAAAAATTGCTAGAGGCCCACGAGGAGCA960.37415231117000547No Hit
AACAAAGGGGAGGAAGGCGCCGGCCTTGGAGGCGCCACGTCCCTTGCGGCGG950.3702548912619846No Hit
AATTTTGGGCAAGTGTTTTAGGATTAGCCTAAAATTGGACATTGTCATTGAT940.3663574713539637No Hit
AATCATGGGGGCATACGTATTGTTAAATGATAAGGTTTAGAATGACAGGAAC920.3585626315379219No Hit
ACTTACGGGACTGATTTCCCACCGGGGACTGGACACAAACAGGAATGTCTGG920.3585626315379219No Hit
TCTCGTGGGGATCTATGTAAGGGATGATAAAAAAATAAAAACAGCCCAACCG880.3429729519058383No Hit
TCTGGAGGGCCTTCCTCATATGAATAACCATTTATATGTGTTTTGCTTAAAG860.33517811208979653No Hit
ATCGCAGGGCGTAGAAGAGAACTTCATATTCGAACATATGCAGTTATTCCAC850.3312806921817757No Hit
TAGCCGGGTATTTTAAAATTTATAAACAAGTCACTTAAATGCCAGTTGTCTG850.3312806921817757No Hit
TCATACGGGAAACTTTGTGAGCTACGTGCAACTGACGTCGCGGGCGCTGCCC820.319588432457713No Hit
ATCAACGGGACTGCCCAAGGACCAGTATTTGTCACTACCAAACTCAGCCCTT820.319588432457713No Hit
GTTCGTGGGCTACGACCACATCAACGAGGGGAAGCTGTGGAAACACATCAAG800.3117935926416712No Hit
TACTATGGGAAGATTTAGGCATAATCTGGTTTTAAAACCTTGATTGAAACTG800.3117935926416712No Hit
CACTTCGGGATCATCTTGAACATTAATCCACATGTTTCAGAGCTCACCAGGC790.30789617273365033No Hit
TGGAAGGGGATGCATTACTCAGGTTGGTGGGGTCGGTTTGAGAAGATATAGA780.30399875282562944No Hit
GCACCCGGGAACTTGAATGGCCTTAAACCTGTTTCAGCTTTAACAGTAGAAT760.29620391300958765No Hit
ACCAAGGGGATTTTTGTTGTTAAAGATTATTTTTAAATAGTAGTAATGAAGT750.29230649310156676No Hit
ATTATGGGGAGATAGAAGCGATGAAGATAAAGAAAAAGCAAGTTCTTTGATC750.29230649310156676No Hit
ATCCACGGGATCTGTCTGGTAGCAGTGAGACCCCTTGTCTTGGTGATCCTTA740.28840907319354586No Hit
TCTGCGAGGGATGCACACATATATTTTCCCGCATAACCGTATCCAATGCTAG730.28451165328552497No Hit
GAAAAAGGGTGGTGCAAACAAACTCAAGTGCAAAGGAAACGCGACGAAGAAC710.2767168134694832No Hit
TTCGCCGGGCGCAGGAAGTCCCTCAAGCAGAAGAAGAAGCGGTAATGCGCCC680.2650245537454205No Hit
CTTGTAGGGGCCTCTTTCCGTCTCAGGTCGCCGCTGCGGAGGGAGCCGCCGC670.26112713383739966No Hit
GTTGCAGGGTGAGGGAGACTGATCGACCCTATCTCAAATCCACTGACGTAGC670.26112713383739966No Hit
TGTTTTGGATGAATGTGCAAGAGGCCTGTGACCGAATGCTACGTTTTTATGG660.2572297139293787No Hit
CGGCAAGGGGTTGGCCAGGATGGTCCTGATCTCCTGACCTTGTGATCCGCCT660.2572297139293787No Hit
CACCTAGGGACACAAAAAGAAAATACATATGCAGTCTAGTCAGACAAAATAA660.2572297139293787No Hit
TCAGTTGGGCGATCTGATTTGGGGCTAGCACAAAAATTGGGGGATGTTAAAA660.2572297139293787No Hit
ACTTCAGGGTGAACACCCGCTGATCCTTTAACAAGGATTTCTGGCAGGAAAC650.2533322940213579No Hit
GATGGCGGGGTTGTTCTTAAATCAAAAATTGGATAATTTTTAATATTTATGT650.2533322940213579No Hit
GCAGTAGGGGTGGTGAAAAACGCGGGGATCCAAAGAGCTGGATTTTTATCTC650.2533322940213579No Hit
ATTCATGGGTCTATGTTAAATTTTTTTCTGCAGAAGAAAATAATCATATTAC650.2533322940213579No Hit
TCTGATGGGAGGAGGTGGCGGCGGTGGCGCTGGGAGCTCCTGTCACCGCTGG650.2533322940213579No Hit
GCCAGCGGAAATGAATCTGAGGAGGCTACTCAGTAGACAGTCCCACCCTCAC630.24553745420531609No Hit
GAAAACGGGAGGTTGGGGGTTGCGTCGCTCTCTGGTAAAGGCGTGCAGGTGT620.24164003429729522No Hit
GAAATGGGGAGCATTGTGAGCGTAACAGCCTACATTGCCTTGGCCCTGCTCT610.2377426143892743No Hit
TCATTAGGGTCAAAAGTTTTGCTTTAGAAATAACTACATAGCCTAGAATTTT610.2377426143892743No Hit
GCTGAAGGGAGGTGTTATTTATTAACGTATTATAAAATAATGTTTGATCCAG600.23384519448125343No Hit
GTAACCGGGGAGTGGTCAAGGCCGCGCTCGCGGCGAGGGGCTGCGAGAGTGA600.23384519448125343No Hit
AAGCCCGGGTCCATTTTAATTTCCTCAGTGGTTTTATGAGAAGTTTTATGGG590.2299477745732325No Hit
GCCCCGGGCGCAGCCTGACCGGGACCCCTCCCTCCCACAGCCCAGCCCACCT590.2299477745732325No Hit
TCGTCAGGGTTGTGACTAATTGTATAACAGGTTATTTTAGTTTCTGTTCTGT590.2299477745732325No Hit
TCTCCTGGGCAGAAAAAAAATGCAAGTGACTAAATTCAATTATTGACTAATT570.22215293475719072No Hit
TCCGTAGGGACTGAAAATACAAAAAATTAGCCAGGCGTGGTGGCGGGCACCT570.22215293475719072No Hit
TTACAAGGGCAACTGCATATTGAAATTTGTTAAAGCACTCATCTTTATGGAA570.22215293475719072No Hit
CCAAGGGGCAGCTTTTCTTGTTGAGCAGAACTATCATCTGCTTTCCCAGATC570.22215293475719072No Hit
TTGGTACGGGGCAGACTTGAGGTGATGATAGTTGGCCTCTGGTCTACAAAGA560.21825551484916986No Hit
GGCCATGGGAGAATATGCTTTCTTGGACCATGTTTGAGACTCAGAAGAATGG550.21435809494114894No Hit
TTTGGCGGGGATCTGGAGAGCGGCGGGCTGCTGCATGAGATTTTCACGTCGC550.21435809494114894No Hit
CAGACTGGGAAACTTTGTGAGAGGTCTTATATTTGAATGGACCCTTAATGAT540.21046067503312807No Hit
CGACTGGGGGAGGAGTCCGAGGTGGGGTTGGTTACCTGTCCCGAGGGGACCC540.21046067503312807No Hit
ATGCATGGGGCAAATTATGACTGCGTGAGCCTTAGAAAATAAAATGTATAAA540.21046067503312807No Hit
TTTCTGGGGATGGTCCCACTTCCCCATCTGTGTGTGAATTCCTTTAGACTAA540.21046067503312807No Hit
AGGTTAGGGATTCATTAAATATTTAATAAAATTTACCAGTACATTTACATAA530.20656325512510718No Hit
TTCCTTTGGGGAGCCCTGGCAGCTGTCTCCAGAGGATCAAAGCCACACCCAA530.20656325512510718No Hit
GATTATGGGGGGAGGCCAAGTCTATGATACTGCCTCAAAGAGACCCTATTGT530.20656325512510718No Hit
GTGATGGGGGATTCCAGCAAAGTCTCTAACTGCAGCCTGTAGACAATTTGCT520.2026658352170863No Hit
GCGCTTGGGGGTGTTCACATATCCCTTTCTCTAACAGTTTAACCTAGACAAA520.2026658352170863No Hit
TAATAGGGGGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCA500.19487099540104452No Hit
GTTACTGGGTGCTAAGGGATACAGGGTTTCTTTTGGGGGGATGCGAATGTTC500.19487099540104452No Hit
ATCGCGGGATGCCGGGAAGGGGCATTATTTGTGCACTGAGAACACCGCGCAG490.19097357549302363No Hit
CGTAACGGGAGCTTAAAACTAGTCAAGCAGTTTAGAACCAAAGGCCTATATT480.18707615558500273No Hit
TCGCCAGGGCGATCTCATCAAGGGTGTTGGAAGTGACCCAGCCCCCTCCCCT480.18707615558500273No Hit
ATCGAGGGGGTGTTTTGTAGACTAGTGTTCTTCCATCCTTCAACTGAGCTCA440.17148647595291916No Hit
GTCGCACGGGATTTGACCGGGACCTAGTGGTCACTCAGTGTGGCAATGCTAT440.17148647595291916No Hit
GTTAGCCGGGTATTTTGGTAATGCTTATCTTCAGTTTAAACATTTATTCTTT420.16369163613687737No Hit
ACTACGGGAGATTCACACTTTCTGGCCTAAACCCTAATGGGATGAGGCTTTT410.1597942162288565No Hit
TCAAAAGGGGCTGCTTGTACACTCATTCATTACTAAAAGTTTTGAGAAATTT410.1597942162288565No Hit
GAAATGGGGTTAGATTCTTTAGGGTATAGCAAATGTGTTCTTATTGGCCATG410.1597942162288565No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCT400.1558967963208356No Hit
AAGCATGGGGACAAGGAACTTATTGATCATTCTCCGAAAGCAACTCCTGACA400.1558967963208356No Hit
ACGAGGGGGAAGTATTCATAGAGGTAGACTGTATGATAAATAAAAACAAATT400.1558967963208356No Hit
GCCCTGGTGGACAATTTCTTCACGGGCAGGAAGAGAAACGTGGAGCACTGGA390.15199937641281472No Hit
TAGATGGGGCTTTGCAGCCTCCTCCATGAATCCCAATGGCCTGCACTTGTAC380.14810195650479382No Hit
AATATTGGGCGGGTACTCACACACGCCCCCCAGCCCAGCCATACGACAGCAG380.14810195650479382No Hit
TATACCGGGTATACAGTCATTGAATAAATACCATGTAGCAAGTGTACTTTGT380.14810195650479382No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTG380.14810195650479382No Hit
TATCGGGTCTGAAGGTGGGTCTAGCTACGGGGTTAGGACCTTCCTGTAAACT370.14420453659677293No Hit
ATAGCTGGGGAGGCTTGTTCCTCGACTTCCCACTCTTCCCATATCTGGTTAA370.14420453659677293No Hit
ATTTGCGGGATTGAAAAGTTGGGAAATGCCTCACGCCATAAATACTAATTGA370.14420453659677293No Hit
GCTAAAGGGAAGAGATACTTACAGATGTTGAGATGGCTGCCCTGCATTTCCA370.14420453659677293No Hit
CTATGTGGGCATAAGCAAGATGGTAAAAATCTGGAAATTGAACTTAGCTCAT370.14420453659677293No Hit
TGATAAGGGACTACAATGATACTATCATTTAATAATATTAATATTACTTGAA370.14420453659677293No Hit
TACCAGGGGTATACGCGGGATGCACAAGACAATCAAATATAAATCTGTCCTC370.14420453659677293No Hit
CACAGCGGGAGATAACTTTTGTAGCAGTGGTTATATTGCTTATAATTTAATG360.14030711668875204No Hit
TATCCGGGGACCCTATTCTATCATTTGCTGTTGCTTGCTGAACTGTGAAGAA350.13640969678073114No Hit
CGTCCGGGAAGACATGAAAACAAGACTGGGTAGTTGTAGATTTCTGCTTTTT350.13640969678073114No Hit
GTTCAAGGGAGCTTGACTGCTTTTAAATGACCAAAGATGACCTGTGGTAAGC350.13640969678073114No Hit
CCGTGGGATTTCCATAAAGAAAACTCAAGTATTAATAAAAGAGACTTTACTG350.13640969678073114No Hit
TAAATGGGGGGCATTGCCGTGGCAACTGAACGATCCTAACTACTTCAGTATT350.13640969678073114No Hit
AGCACAGGGGGAATATTTTAGATTCTTGGGTATCTGATAATCTGGAATTTCA350.13640969678073114No Hit
TAGAAAGGGGAACCAGACTTGCTAAGCCAGTTCTATGCTTGGGCTTAATGTG350.13640969678073114No Hit
CATCGAGGGATTTAAAATTGGAATGTGAGACATGTTGCTGTGACCTGTTTTT340.13251227687271025No Hit
TTATAGGGATTTGCTGACTTCTTTATAATGAATCTGATCCTCTGGGGAGAAG340.13251227687271025No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGA330.12861485696468936No Hit
GAATCCGGGAGTCGCGGACGCAATGGCTTCAAGGTTACTTCGCGGAGCTGGA330.12861485696468936No Hit
TTGAACGGGTTCAGTAATGACTGAGAATTGAATACAAATAGATTACAAAGCC330.12861485696468936No Hit
AGATAGGGATGATAAATGAATCATTGCCTCACTTCGAAGATTTCTGATGAAC330.12861485696468936No Hit
TTGCTTGGGGACGCTCTTCCGATCTGACATTGGGAGAATGAAGAGCAGCAGT310.12082001714864761No Hit
TCTCGAGGGAATTGAAGTCTGCTTCTAAAAAAGTACAAAGTCATACTCATTA310.12082001714864761No Hit
CTCATAGGGTAACCAAGGAGGTGAAAGACCTCTACAAGGAAAACTACAAAAC310.12082001714864761No Hit
ACAGGGGGAAACTATGTAAACTTCATTTTTCATGCAGTGCCCAACTACTGCT300.11692259724062672No Hit
CAAAAAGGGTGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTC300.11692259724062672No Hit
TATTTAGGGGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTAG300.11692259724062672No Hit
TCCCAGGGACTGGTGGATAATTTATGTGATGTGGATTGCTGGTGTCCAGCAT290.11302517733260582No Hit
GTGTATGGGGTATGGTCATATTAGTAGCTATTTGTTGCAAGATACTTGAAGC290.11302517733260582No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCTTATAC290.11302517733260582No Hit
GTTCATGGGTGGGAAACACTCTACTATTTATGTAGTCAATATAACAAACAAG290.11302517733260582No Hit
GTAGTGGGGATGTCTGGGCTCTCATTACCCAAACATCTGAGATTCCATTTTT280.10912775742458493No Hit
CAACTTGGGACGCGGGCGGCGCAGTCATTCTCATAATCGCCCACGGGCTTAC280.10912775742458493No Hit
GTAACTGGGGCCGCCTCACTTCGCGCACGCGCCGCTAGGCTGGGGGAGTTGA270.10523033751656403No Hit
GGACATGGGACAAGATTTCTTCTAATAAGTGGTTTATTTAAACTCAATTTCG270.10523033751656403No Hit
TCGTGCGGGGCTGAGAACGCGGGTCCACGCGTGTGATCGTCCGTGCGTCTAG270.10523033751656403No Hit
CATATAGGGACTGTTAAAACTGTACCTTTTGCGATTTCACAGTTGGCACTTC260.10133291760854315No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)