FastQCFastQC Report
Sat 31 Dec 2022
SRR5845316.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845316.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25123
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAAGGGATGGGTGAGTTTTTTTCCTTGACCTACTTAGAAAGGTATCTTT2060.8199657684193766No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC1990.7921028539585241No Hit
CCGCATGATGAAACTTCGGCTCACTCCTTGGCGCCTGCCTGATCCTCCAAAT1900.7562791067945708No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT1830.7284161923337181Illumina PCR Primer Index 5 (95% over 21bp)
TACGGCGGGTCCCTATTGTTGGCTCCACAGCCTTAAAGTGCTATAGATTTAA1600.6368666162480595No Hit
ATCCTAGGGAAAGAGAATTCAAAAAGGGCTGATGGTAGGCTTTGAACATGGG1440.5731799546232536No Hit
AACTTTGGGAGAACCTGCATTTAAGAACAGAACAGCAAGTATGAACCACATG1440.5731799546232536No Hit
TTTCTTGGGAGGCCCCAACGTTGTAGGCCCCTACGGGCTACTACAACCCTTC1420.5652191219201529No Hit
TTCGCTGGGGGAGGAGGAGGGACTCGGCGGGAGGATGGGCTTGCTCTCAATT1400.5572582892170521No Hit
CTTTAGCCGGGCCATCGCTGGGTCAATAGTACTTGCCGCAGTACTCTTAAAA1340.5333757911077499No Hit
GTAAATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1340.5333757911077499No Hit
AGTTTTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1330.5293953747561995No Hit
CTCATCGGGGTTGGTTTGCCTTGCAAAACAGAAAAATGGAATTTTAGCTCAA1310.5214345420530987No Hit
TCTATAGGGATGGCTACCTTAAACAACATCAGAAAATTCATACTGGCATGAA1310.5214345420530987No Hit
TCAAACGGGCATAATGCTTCGTGACGGCCACCAGGACAGAACCACCTGATGT1270.5055128766468973No Hit
ACTGCTGGGAAAGGAATTTTAACAAAGAGCAATCAGTATTATTGGACCAAAT1270.5055128766468973No Hit
GACCTAGGGCGAAAAATTCTAGGCTATATACAACTACGCAAAGGCCCCAACG1250.4975520439437965No Hit
GTGTAAGGGAGGCAGAGAAAACATCAGGGAAAAGGAAGGTCCTGACTCCCTG1250.4975520439437965No Hit
GGCAACGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1240.49357162759224615No Hit
CCAACAGGGATGAGCTCTGAGTCCATTAAAATACAGAATGAACATTTAAGAA1240.49357162759224615No Hit
CAATTTGGGCTGGGGGTCAGAGCGAAGAAGAGGCATCATTCAACCTCAATGC1230.48959121124069577No Hit
GTTGTTAGGGGAGGACGTCCAACTTCTCCCTGATGACGGAGCTGAGCATCAA1230.48959121124069577No Hit
CTATCTGCGGGATTCTGATAAAATCTCTTGAAAATACTGAAATCAAAAGGTT1190.4736695458344943No Hit
TTGTAGGGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATA1180.46968912948294395No Hit
GACCTTGGGGCCCGCCACCATGCCCGACTAATTTTTTTTGTATTTTTAGTAG1160.4617282967798432No Hit
TATAAAGGGGCTCATAAGTAAATGTCTTCATGTTGATGTGTCCGTGAAAGTT1160.4617282967798432No Hit
CTAGCCAGGGGATGGCTGAGGGCAACCCGCCCACGGAACTGACGCAGTCGCA1160.4617282967798432No Hit
CCTAAAGGGGTGATCTCTCGGTGGGGGGCCCATCTCTGCTGACCTGGGTGTG1100.43784579867054096No Hit
CTGGACGGGACTTACAAGCTGCAGATAGAATAAAACTATTCTTTATGTTTTT1090.4338653823189906No Hit
AATCATGGGGGCATACGTATTGTTAAATGATAAGGTTTAGAATGACAGGAAC1080.42988496596744025No Hit
ATCCAGGGGTCCGATCTCTAGAAGGGATTTGCAGGCTTTTCTGAATACCAAA1070.4259045496158898No Hit
TTCACAGGGGGACAAGATCGACGTGATCAAGCAGGCTGACTATGTGCCGAGC1050.4179437169127891No Hit
CTTATCGGGGGGCTGACAGAGCAGGTTCTCATTTCTCAGCTCTTTCCCTTAT1040.41396330056123865No Hit
ATATAAGGGATTTCCCTCAGTACTGTAAGCAAAAGTGATTACGGGCAGGGAC1040.41396330056123865No Hit
GCTATAGGGGGAGGGAACATTCTGCAGTATAAAGGGAGCAGGGAAGGCGGGA1030.4099828842096883No Hit
AGCATAGGGACTACACTGTCTTATATACTCGTAACTTCCCCGTAGGTCTTGA1030.4099828842096883No Hit
GGCCAAGGGAATGAGAAGAAGCACAATTTTGATTGTGATGAGTCACTTTCTG1020.4060024678581379No Hit
CTTGCCGGGTCTTCCGATCTCTATATGGGGTCATACATTCTTCTTGCTGTTG970.3861003861003861No Hit
AACAAAGGGGAGGAAGGCGCCGGCCTTGGAGGCGCCACGTCCCTTGCGGCGG960.3821199697488357No Hit
AAGATAGGGAGAACAGAAAGTGAGGGGCTCTAAGCAGATGAATACACTCCAG950.3781395533972854No Hit
TCTCGTGGGGATCTATGTAAGGGATGATAAAAAAATAAAAACAGCCCAACCG950.3781395533972854No Hit
ACTAGAGGGGAGCTCAAGCCCGCAGCTCGCAGGGAGATCCAGCTCCGTCCTG930.3701787206941846No Hit
GACAGCGGGCTCCCTCTTAGATTCTTGTACCAAGCTTCTTCAGAAAGGGATT930.3701787206941846No Hit
ATAAAAGGGAGATCTATTAATGTAGGGAATTTTTCCCTAAGATTGAATTCCT920.36619830434263423No Hit
TATATTGGGGACAGACTAGAAGTTAAGTTTAATACAGAAAATGTCCTGTTCA920.36619830434263423No Hit
ATCGCAGGGCGTAGAAGAGAACTTCATATTCGAACATATGCAGTTATTCCAC900.3582374716395335No Hit
CACCTAGGGACACAAAAAGAAAATACATATGCAGTCTAGTCAGACAAAATAA880.3502766389364328No Hit
ACTTACGGGACTGATTTCCCACCGGGGACTGGACACAAACAGGAATGTCTGG880.3502766389364328No Hit
ATCAACGGGACTGCCCAAGGACCAGTATTTGTCACTACCAAACTCAGCCCTT870.3462962225848824No Hit
AACGGGGGGAATGCAAGTTGATGAAAAAATTGCTAGAGGCCCACGAGGAGCA840.33435497353023125No Hit
ACATAAGGGGGAGGGCGGGGCCGGGCCCGGGAGCCTGTGGCTTCAGGAAGAG840.33435497353023125No Hit
AATTTTGGGCAAGTGTTTTAGGATTAGCCTAAAATTGGACATTGTCATTGAT820.3263941408271305No Hit
GCACCCGGGAACTTGAATGGCCTTAAACCTGTTTCAGCTTTAACAGTAGAAT810.32241372447558014No Hit
TTGGCCGGGAGGGGGTGGGAAGGTAGGATGTGGAGTGACATGGTTCTATCCT810.32241372447558014No Hit
TCTGCGAGGGATGCACACATATATTTTCCCGCATAACCGTATCCAATGCTAG770.30649205906937865No Hit
TAGCCGGGTATTTTAAAATTTATAAACAAGTCACTTAAATGCCAGTTGTCTG760.30251164271782827No Hit
CCAAGGGGCAGCTTTTCTTGTTGAGCAGAACTATCATCTGCTTTCCCAGATC760.30251164271782827No Hit
ACTTCAGGGTGAACACCCGCTGATCCTTTAACAAGGATTTCTGGCAGGAAAC750.2985312263662779No Hit
ATCCACGGGATCTGTCTGGTAGCAGTGAGACCCCTTGTCTTGGTGATCCTTA750.2985312263662779No Hit
TCATACGGGAAACTTTGTGAGCTACGTGCAACTGACGTCGCGGGCGCTGCCC740.29455081001472755No Hit
GAAAACGGGAGGTTGGGGGTTGCGTCGCTCTCTGGTAAAGGCGTGCAGGTGT720.2865899773116268No Hit
TCTCCTGGGCAGAAAAAAAATGCAAGTGACTAAATTCAATTATTGACTAATT710.28260956096007644No Hit
TCAGTTGGGCGATCTGATTTGGGGCTAGCACAAAAATTGGGGGATGTTAAAA700.27862914460852606No Hit
GTTCGTGGGCTACGACCACATCAACGAGGGGAAGCTGTGGAAACACATCAAG690.27464872825697567No Hit
ATTCATGGGTCTATGTTAAATTTTTTTCTGCAGAAGAAAATAATCATATTAC690.27464872825697567No Hit
ATTATGGGGAGATAGAAGCGATGAAGATAAAGAAAAAGCAAGTTCTTTGATC690.27464872825697567No Hit
GAAAAAGGGTGGTGCAAACAAACTCAAGTGCAAAGGAAACGCGACGAAGAAC680.2706683119054253No Hit
TTCGCCGGGCGCAGGAAGTCCCTCAAGCAGAAGAAGAAGCGGTAATGCGCCC680.2706683119054253No Hit
GTGATGGGGGATTCCAGCAAAGTCTCTAACTGCAGCCTGTAGACAATTTGCT670.26668789555387495No Hit
GATTATGGGGGGAGGCCAAGTCTATGATACTGCCTCAAAGAGACCCTATTGT670.26668789555387495No Hit
CGGCAAGGGGTTGGCCAGGATGGTCCTGATCTCCTGACCTTGTGATCCGCCT660.26270747920232457No Hit
TGGAAGGGGATGCATTACTCAGGTTGGTGGGGTCGGTTTGAGAAGATATAGA660.26270747920232457No Hit
GAAATGGGGAGCATTGTGAGCGTAACAGCCTACATTGCCTTGGCCCTGCTCT650.2587270628507742No Hit
TCCGTAGGGACTGAAAATACAAAAAATTAGCCAGGCGTGGTGGCGGGCACCT650.2587270628507742No Hit
TCTGGAGGGCCTTCCTCATATGAATAACCATTTATATGTGTTTTGCTTAAAG650.2587270628507742No Hit
GCAGTAGGGGTGGTGAAAAACGCGGGGATCCAAAGAGCTGGATTTTTATCTC650.2587270628507742No Hit
GTTGCAGGGTGAGGGAGACTGATCGACCCTATCTCAAATCCACTGACGTAGC640.25474664649922385No Hit
CTTGTAGGGGCCTCTTTCCGTCTCAGGTCGCCGCTGCGGAGGGAGCCGCCGC620.24678581379612308No Hit
TTTCTGGGGATGGTCCCACTTCCCCATCTGTGTGTGAATTCCTTTAGACTAA610.24280539744457272No Hit
TACTATGGGAAGATTTAGGCATAATCTGGTTTTAAAACCTTGATTGAAACTG600.23882498109302233No Hit
TCGCCAGGGCGATCTCATCAAGGGTGTTGGAAGTGACCCAGCCCCCTCCCCT590.23484456474147197No Hit
TCATTAGGGTCAAAAGTTTTGCTTTAGAAATAACTACATAGCCTAGAATTTT590.23484456474147197No Hit
ACCAAGGGGATTTTTGTTGTTAAAGATTATTTTTAAATAGTAGTAATGAAGT590.23484456474147197No Hit
ATGCATGGGGCAAATTATGACTGCGTGAGCCTTAGAAAATAAAATGTATAAA590.23484456474147197No Hit
GCTGAAGGGAGGTGTTATTTATTAACGTATTATAAAATAATGTTTGATCCAG580.2308641483899216No Hit
ACGAGGGGGAAGTATTCATAGAGGTAGACTGTATGATAAATAAAAACAAATT580.2308641483899216No Hit
TTCCTTTGGGGAGCCCTGGCAGCTGTCTCCAGAGGATCAAAGCCACACCCAA570.22688373203837123No Hit
CACTTCGGGATCATCTTGAACATTAATCCACATGTTTCAGAGCTCACCAGGC560.22290331568682087No Hit
GCCAGCGGAAATGAATCTGAGGAGGCTACTCAGTAGACAGTCCCACCCTCAC560.22290331568682087No Hit
GCCCCGGGCGCAGCCTGACCGGGACCCCTCCCTCCCACAGCCCAGCCCACCT550.21892289933527048No Hit
AGGTTAGGGATTCATTAAATATTTAATAAAATTTACCAGTACATTTACATAA550.21892289933527048No Hit
TGTTTTGGATGAATGTGCAAGAGGCCTGTGACCGAATGCTACGTTTTTATGG540.21494248298372012No Hit
TCAAAAGGGGCTGCTTGTACACTCATTCATTACTAAAAGTTTTGAGAAATTT540.21494248298372012No Hit
TCGTCAGGGTTGTGACTAATTGTATAACAGGTTATTTTAGTTTCTGTTCTGT540.21494248298372012No Hit
TTGGTACGGGGCAGACTTGAGGTGATGATAGTTGGCCTCTGGTCTACAAAGA530.21096206663216974No Hit
TAATAGGGGGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCA520.20698165028061932No Hit
CGTAACGGGAGCTTAAAACTAGTCAAGCAGTTTAGAACCAAAGGCCTATATT520.20698165028061932No Hit
GTAACCGGGGAGTGGTCAAGGCCGCGCTCGCGGCGAGGGGCTGCGAGAGTGA520.20698165028061932No Hit
GATGGCGGGGTTGTTCTTAAATCAAAAATTGGATAATTTTTAATATTTATGT510.20300123392906896No Hit
ATAGCTGGGGAGGCTTGTTCCTCGACTTCCCACTCTTCCCATATCTGGTTAA510.20300123392906896No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTG510.20300123392906896No Hit
CAGACTGGGAAACTTTGTGAGAGGTCTTATATTTGAATGGACCCTTAATGAT500.19902081757751858No Hit
AAGCCCGGGTCCATTTTAATTTCCTCAGTGGTTTTATGAGAAGTTTTATGGG500.19902081757751858No Hit
ATCGCGGGATGCCGGGAAGGGGCATTATTTGTGCACTGAGAACACCGCGCAG500.19902081757751858No Hit
TAGAAAGGGGAACCAGACTTGCTAAGCCAGTTCTATGCTTGGGCTTAATGTG500.19902081757751858No Hit
CGACTGGGGGAGGAGTCCGAGGTGGGGTTGGTTACCTGTCCCGAGGGGACCC490.19504040122596825No Hit
ACTACGGGAGATTCACACTTTCTGGCCTAAACCCTAATGGGATGAGGCTTTT480.19105998487441786No Hit
GGCCATGGGAGAATATGCTTTCTTGGACCATGTTTGAGACTCAGAAGAATGG480.19105998487441786No Hit
AAGCATGGGGACAAGGAACTTATTGATCATTCTCCGAAAGCAACTCCTGACA460.18309915217131711No Hit
CAAAAAGGGTGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTC460.18309915217131711No Hit
GTTACTGGGTGCTAAGGGATACAGGGTTTCTTTTGGGGGGATGCGAATGTTC460.18309915217131711No Hit
TACCAGGGGTATACGCGGGATGCACAAGACAATCAAATATAAATCTGTCCTC460.18309915217131711No Hit
TTGCTTGGGGACGCTCTTCCGATCTGACATTGGGAGAATGAAGAGCAGCAGT450.17911873581976676No Hit
TTTGGCGGGGATCTGGAGAGCGGCGGGCTGCTGCATGAGATTTTCACGTCGC450.17911873581976676No Hit
CACAGCGGGAGATAACTTTTGTAGCAGTGGTTATATTGCTTATAATTTAATG450.17911873581976676No Hit
GAAATGGGGTTAGATTCTTTAGGGTATAGCAAATGTGTTCTTATTGGCCATG450.17911873581976676No Hit
TCTGATGGGAGGAGGTGGCGGCGGTGGCGCTGGGAGCTCCTGTCACCGCTGG450.17911873581976676No Hit
TAAATGGGGGGCATTGCCGTGGCAACTGAACGATCCTAACTACTTCAGTATT450.17911873581976676No Hit
GTCGCACGGGATTTGACCGGGACCTAGTGGTCACTCAGTGTGGCAATGCTAT440.1751383194682164No Hit
CATCGAGGGATTTAAAATTGGAATGTGAGACATGTTGCTGTGACCTGTTTTT440.1751383194682164No Hit
TCTCGAGGGAATTGAAGTCTGCTTCTAAAAAAGTACAAAGTCATACTCATTA440.1751383194682164No Hit
GCGCTTGGGGGTGTTCACATATCCCTTTCTCTAACAGTTTAACCTAGACAAA440.1751383194682164No Hit
ACAGGGGGAAACTATGTAAACTTCATTTTTCATGCAGTGCCCAACTACTGCT430.17115790311666598No Hit
TATCGGGTCTGAAGGTGGGTCTAGCTACGGGGTTAGGACCTTCCTGTAAACT420.16717748676511562No Hit
CGTCCGGGAAGACATGAAAACAAGACTGGGTAGTTGTAGATTTCTGCTTTTT410.16319707041356524No Hit
TTGAACGGGTTCAGTAATGACTGAGAATTGAATACAAATAGATTACAAAGCC400.15921665406201488No Hit
CCGTTTGGGACTGGTGGATAATTTATGTGATGTGGATTGCTGGTGTCCAGCA400.15921665406201488No Hit
TATTCTGGGGTGTATGGTTTGCAGCTTTTAAAAACCATTTTTTTAAAACTAA380.15125582135891413No Hit
CAACTTGGGACGCGGGCGGCGCAGTCATTCTCATAATCGCCCACGGGCTTAC380.15125582135891413No Hit
ATCGAGGGGGTGTTTTGTAGACTAGTGTTCTTCCATCCTTCAACTGAGCTCA370.14727540500736377No Hit
TTACAAGGGCAACTGCATATTGAAATTTGTTAAAGCACTCATCTTTATGGAA370.14727540500736377No Hit
GTTAGCCGGGTATTTTGGTAATGCTTATCTTCAGTTTAAACATTTATTCTTT360.1432949886558134No Hit
TATTTAGGGGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTAG360.1432949886558134No Hit
TATCCGGGGACCCTATTCTATCATTTGCTGTTGCTTGCTGAACTGTGAAGAA350.13931457230426303No Hit
AATATTGGGCGGGTACTCACACACGCCCCCCAGCCCAGCCATACGACAGCAG350.13931457230426303No Hit
GAATCCGGGAGTCGCGGACGCAATGGCTTCAAGGTTACTTCGCGGAGCTGGA350.13931457230426303No Hit
TATACCGGGTATACAGTCATTGAATAAATACCATGTAGCAAGTGTACTTTGT350.13931457230426303No Hit
ATTTTAGGGAGAGATGCTGATGAAGAAAGAGCCTAGCGCACGTGGGAAACTG330.13135373960116228No Hit
GTTCAAGGGAGCTTGACTGCTTTTAAATGACCAAAGATGACCTGTGGTAAGC330.13135373960116228No Hit
CACTGCGGGACCCATGTCCCAGGTGTATGGAGCGCCACATCTCCTGAGATTA330.13135373960116228No Hit
CTATGTGGGCATAAGCAAGATGGTAAAAATCTGGAAATTGAACTTAGCTCAT330.13135373960116228No Hit
GTCCGCGAGGGGCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCAT320.12737332324961192No Hit
GTAGTGGGGATGTCTGGGCTCTCATTACCCAAACATCTGAGATTCCATTTTT320.12737332324961192No Hit
CTCCGTGGGATATCTGTAAAGATAATCGAAGCAAAAGTAATCACTTAAAGGC320.12737332324961192No Hit
GGACATGGGACAAGATTTCTTCTAATAAGTGGTTTATTTAAACTCAATTTCG320.12737332324961192No Hit
TTATAGGGATTTGCTGACTTCTTTATAATGAATCTGATCCTCTGGGGAGAAG310.12339290689806154No Hit
GCCCTGGTGGACAATTTCTTCACGGGCAGGAAGAGAAACGTGGAGCACTGGA300.11941249054651117No Hit
TGATAAGGGACTACAATGATACTATCATTTAATAATATTAATATTACTTGAA300.11941249054651117No Hit
AGTTATGGGCAGCACTCCCATACCTCCTGCTCTCACTGTTTGCCTGCCAAGT290.1154320741949608No Hit
TGAGCTCGGGGTAAGGAAAAATTTTACCTGAAAACAAACAAACAAACCCTAA290.1154320741949608No Hit
GTTCATGGGTGGGAAACACTCTACTATTTATGTAGTCAATATAACAAACAAG290.1154320741949608No Hit
ACGCGGGAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTAT290.1154320741949608No Hit
ACTGTCGGGGAATTCTTGCCCTTAAGTGCTTTTGCTGCTATTTTTTTGCCCC280.11145165784341043No Hit
TAGATGGGGCTTTGCAGCCTCCTCCATGAATCCCAATGGCCTGCACTTGTAC280.11145165784341043No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCTTATAC280.11145165784341043No Hit
ATTTGCGGGATTGAAAAGTTGGGAAATGCCTCACGCCATAAATACTAATTGA280.11145165784341043No Hit
GATGTCGGGTGTAACTAACCTGCACAATGTGCACATGTACCCTAAAACTTAA280.11145165784341043No Hit
GTTGCCGGGATGCATCACTTCCCCCAGTATAAATCAGGAATGTTTGTGAGAA270.10747124149186006No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCT270.10747124149186006No Hit
GTGTATGGGGTATGGTCATATTAGTAGCTATTTGTTGCAAGATACTTGAAGC270.10747124149186006No Hit
AGATAGGGATGATAAATGAATCATTGCCTCACTTCGAAGATTTCTGATGAAC270.10747124149186006No Hit
TCGTGCGGGGCTGAGAACGCGGGTCCACGCGTGTGATCGTCCGTGCGTCTAG270.10747124149186006No Hit
TCCCAGGGACTGGTGGATAATTTATGTGATGTGGATTGCTGGTGTCCAGCAT260.10349082514030966No Hit
GCTAAAGGGAAGAGATACTTACAGATGTTGAGATGGCTGCCCTGCATTTCCA260.10349082514030966No Hit
CATATAGGGACTGTTAAAACTGTACCTTTTGCGATTTCACAGTTGGCACTTC260.10349082514030966No Hit
GTATCTGGGGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCTAGA260.10349082514030966No Hit

[WARN]Adapter Content

Adapter graph