FastQCFastQC Report
Sat 31 Dec 2022
SRR5845312.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845312.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22528
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTGTGGGCTGATAATGAATGCCAGCTTTCCTTGTGCTTTGATAACAAAGA4842.1484375No Hit
GCATAAGGGAGCTCTCCGGAAGCTAGAAGCAGATGATTTTTTCCAAAGCTGC4782.121803977272727No Hit
TATTCTGGGAGAATACATCCCAGTAAGGGTCCTATGGAGAAAGACCTGTTGT4431.9664417613636365No Hit
TATGTAGGGCGGCGCCGACCAGGAAATTTCGATTTGCACACTGATTTGGCCC4131.8332741477272727No Hit
TGCAGTGGGGGGACAGAACCGTGTCCTCAGGAGCTAACACCTGGGCTCCATC4031.7888849431818181No Hit
ACTTTAGGGTAAAATTTAAACAGATGGGCCTGAATCCAAACAAGGACACAAC3891.7267400568181819No Hit
GGTGCTGGGATACCCAGAAAATTTCATACTACTGTTTTCCAAAGTGAAGTTG3281.455965909090909No Hit
TAGCTGGGATTACAGGTGTTTGCCACCATGCCCAGCTAATTTTTGCATTTTT3111.3805042613636365No Hit
AAATAGGGGGAGTCATCCTGAACTAGTTTGAAATTGTGAAGCTGCTTCTTGG3021.3405539772727273No Hit
CTTAAGGGGGTTAAATTTCTTGTACAAAATTGTTTATTTGCCTTTTCTTTGT2821.2517755681818181No Hit
TCTTGTGGGAATGCCAAATTATTCTTCATAAGAGCTGTTGCCAAATCAGTGA2801.2428977272727273No Hit
AATCGTGGGATTTATGAATAACTGTTGATTTGTAAAGTCATTATGGACTTGG2781.2340198863636365No Hit
AAGCCAGGGCTCGGGCATGGGGGTCGATAATAATTAAGTCTTAATTAGTAAC2731.211825284090909No Hit
TTAGGAGGGGTGTAAAGGTGAACACTGTCACCTCAACCTGTTCCAGGTAAAC2701.1985085227272727No Hit
TTATGAGGGTACAAATGAGCAAGGTATTTGTTGCAACTTACTACATAGCATA2641.171875No Hit
CAAAGGGGGGAAGAAGACACATTTCTAGATTATTTGTCCTTTTTATCCTCTC2601.1541193181818181No Hit
GTGACTGGGTGAGGGCTGGCTATTGAGAACTGGAAAGAGTAAAATGATAACT2601.1541193181818181No Hit
CTCTCTGGGCTTGCAACCTAGTTCATGATAGCAGAGTACATGGGCATGCAGA2581.1452414772727273No Hit
ACGCAGGGGGATACCAAACCCTTATAAATCAAACTCACAAGTTAAAAAAATT2431.0786576704545454No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT2170.9632457386363636Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC2130.9454900568181818No Hit
GTAGCCGGGGAATTGATTGTTGTGTAGGTCTAAGATAAGGGTCTGATTTCAT2100.9321732954545454No Hit
CACAGCGGGAAGTGTTTTATTAATAATTAAATGTGATAATACTTAATGAGAA2090.927734375No Hit
ATCGCGGGGGGAACTAAGCAGTGAACACACTGGGGGAAACGTTTGCTCCAGA1930.8567116477272728No Hit
ACTTGGGGGAAGAAATTGGCAATATCGTATAAAAATCTGCTCATATTAGAAC1910.8478338068181818No Hit
CAGACTGGGATTCATAAGTCATAGAAAATAAATACATCATATAGTTGTATGG1840.8167613636363636No Hit
TCTTGAGGGAACCGGCCTCCCATAAATTCCAATCACATTTCTTCCCTGCTTA1820.8078835227272728No Hit
GTACATGGGGAAAGTGGCAGTCCCAGGACCTACCAGGTAAAGAGATCAGGAC1770.7856889204545454No Hit
GATCGCGGGATTCTAATCTATGCTTGTGATACAGATTTCTTTATATCTTCTT1670.7412997159090909No Hit
CTGGCTGGGATTACAGGTGCGTGCCACCACGCCTGGCTAATTTTTGTATTTT1530.6791548295454545No Hit
TCTAGAGGGTCTGGAATTTCAGTAACAATATGAAATTAACATCATTATCATT1500.6658380681818182No Hit
CTGGCTGGAAGTCCTGATACTGAATCTCCAGTGTTAGTGAATGACTATGAAG1480.6569602272727273No Hit
GTGATGGGGAAGACAATGGAGGGTTTTTTGTTTGTTTGTTTTTACTATAACT1470.6525213068181818No Hit
ATCACCGGGGCACCACTGCCCTCCAGCCTAGGTGACAGAATGAGACTCTATC1440.6392045454545455No Hit
ACGTTAGGGCAGACATCACATGTTCTGACTTATTTATTGGAGCTAAAGATGA1440.6392045454545455No Hit
AACGCCGGGAGGACAGATGGAGCCTCCCTTTCCTCATCTACTCTGTGGGTGG1380.6125710227272727No Hit
TCAGGCGGGATCTGCCAGTGCCTTGGTTTATAGGCCATTTAGTTTATGATAT1320.5859375No Hit
ACTGAAGGGTGGGGACAATGTGATCATCCTTGGAGAGAGATGTGAGCACCAA1300.5770596590909091No Hit
TCAGGTGGGACCCCCAGGCGAGCATCTGACAGCCTGGGTCGGCACCCACACC1260.5593039772727273No Hit
CAAGCCGGGAAAGGAAAGAAAGCAGAATCCAAAACATCAGAATGAATTGAGA1250.5548650568181818No Hit
GCACAAGGGAAGGGGATTGAATCCATAATCATAAATCTGTCAACAGAGAAGA1210.537109375No Hit
CTTCTCGGGAAGGAAGGAGTGGTTAGTAAACTGCACTGTTTCTCTGATAATG1100.48828125No Hit
TGAATGGGCACCAATCACCTCAGCCCTCTACCCTACAGATTTCTAAATCATA1080.4794034090909091No Hit
GGCCAGGGTGGGCGGATTACCTCGGGTCAGGAGTTCGAGACCAGCCTGGTCA990.439453125No Hit
ACAACGGGATGGTTAGTATTGGCTAAGAAAGACTATAAGGTATACATGTTAT990.439453125No Hit
TAGCAGGGGCTGGGAGGCCGAGCTTGCAGTGAGCTGAGATCGCGCCACTGCA880.390625No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTG870.38618607954545453No Hit
ACGACAGGGCTGACATTAAAATTGTCTGATTTTGTCCGTTTCTTAAAATTAT840.3728693181818182No Hit
CACTCCGGGATTGTATATGAGGTTGCAGTGGCTTAAAAAGGAAGGACTTTTG820.36399147727272724No Hit
TCCTACGGGAAGAACTGAAATTTTTAATTAAAATTTATTTCCCCTATTAGGC810.3595525568181818No Hit
CGCAGTGGGGCCCACTGGCTGCTCTGAAAAGCCATCTTTGCATTGTTCCTCA800.35511363636363635No Hit
TATTATGGGGGGAGTTAGAGGCTGTTCCAACCATCCTTAGTTTGGGAGAGTG780.34623579545454547No Hit
GGGCCTGGGAGGCGGAGGTTGCAGTGAGCTGAGATTACACCACTGCACTCCT780.34623579545454547No Hit
GCAAGGGGGCGATCCTATCATGACTTAACATCAAGCGCGCAGTTCAAAACTA770.341796875No Hit
GACAGAGGGTTCTGTATACATGTAGCTTTGCCAGATATGTACTTAGTAATAT750.3329190340909091No Hit
AATATGGGGATGGGAGTAGAAAACAACAAAGAATGGAATGGACTCTTAAAAC610.27077414772727276No Hit
ATGCGGGGGGATTCGTGGGCATGATCCTGATTCTCATCTTCGCCGAGGTGCT610.27077414772727276No Hit
GCTCCCGGGTTCAGCTGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACT610.27077414772727276No Hit
GAGCAGGGGATTTGTTTCCTTTGGATGCCGTATCAAATTACCACAAGCTGAG580.25745738636363635No Hit
GGAGCTAGAAATACCATTTGACCCAGCCATCCCATTACTGGGTATATACCCA570.2530184659090909No Hit
CATAAAGGGCAAGTAGTACCATTTTAAATGTTATTGCAATTTGGAATCTGAA570.2530184659090909No Hit
AAATGGGGGGGAGAAGGGAAAGAAATAAAAGAAGAATACATAGTATATTGAA560.24857954545454544No Hit
AATAATGGGGATCACCAAAGAGGAAGCCTCTGGAAGTTCTGTCACAGCTGAG550.244140625No Hit
AGTTAGGGGGAAAAAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTAT520.23082386363636365No Hit
TTTACAGGGATATCATAGATATGAAGACATAAAGCAGCAGGTTTGGGGTTTT520.23082386363636365No Hit
TTAGCTGGGATACATGTGTGTATATATGTGTATATATACATACACTTGTATA520.23082386363636365No Hit
AGAGATGGGCAGGCCAAGAGTTTGAGATTAGCCCAGGCAACATGGCAAGACC500.22194602272727273No Hit
TTTACTGGGGGGTGATGATTAGAGGAAAGGGTTTTTCTGGTTTGTATGTTGG490.21750710227272727No Hit
TGTTACGGGAGTGTATGGGATATTTATAAATTCAAAGAATCTTTTTTTTATT480.2130681818181818No Hit
TCCCACGGGCTCTACTAAACATACAAAAAATTAGCCAGGTGTGGTGGCACCC460.2041903409090909No Hit
TGGGCAGGGAAAAGGGTCAGGGTTCATCAGGTGAACTCAACACTGGGATGAG450.19975142045454544No Hit
AAGTAGGGGACATCCAGATGGCCTGAAGCAAATGAAGATCCACAAAAGAAGT440.1953125No Hit
GAATAGGGGTTAGCAAGTGAACTTTATAATATTTTAGACAGCTTATATTTAC440.1953125No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCT430.19087357954545456No Hit
CAGCACTTTGGGAGGCCTAGGCAGGCGGATCACCTGACGTTGGGAGTTCGAG430.19087357954545456No Hit
ATAGAGGGGGCCAAACAGATTCACTACTCTTACTGATTCTTCACTGAGCTTT400.17755681818181818No Hit
TTTTAAGGGGGGATAGATAAATCAAGCTAATTATACATATGTATTACCTCAC400.17755681818181818No Hit
ATATAAGGGGGGTTCTTTGTATTGGCACTTTGCACCAGAAACATATCATTAT370.16424005681818182No Hit
GGCCTGACTTGGGGGAACGATGTGTCTGTATTTCATGTGGCTGTAGATCCCA370.16424005681818182No Hit
ATACAAGGGCTCCCACCAACAGTGTAAAACCATTCCTATTTCTCCACAGCCT360.15980113636363638No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGA360.15980113636363638No Hit
TGTAATGGGGTTGCAGTGAGCTGAGATTGCACCACTGCACTGCAGCCTGGGC350.1553622159090909No Hit
AGTCTCAGGCTTCAACATCGAATACGCCGCAGGCCCCTTCGCCCTATTCTTC330.146484375No Hit
CAGTCAGGGATACCATAGTTTATTCAACCAATTGCACATAATGTGGATCTCT330.146484375No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCTTATAC320.14204545454545456No Hit
ATCAAGGGATAGCTGGAATTAAGTGTTGTCTTGGAGCTGTTGTACATTTAAG320.14204545454545456No Hit
AATGCAGGGAATGCAGGAGGACAGTGAAGCAAAGTCAATAGATTTTGTAGAA310.1376065340909091No Hit
GCTAGAGGGGGGGTGATACAAATGTTCTGATAGATAGAGGTGGTGGCTGCAT310.1376065340909091No Hit
GTTTTGGGGCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCT290.12872869318181818No Hit
GCCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTAAGCCACCGTGCC280.12428977272727272No Hit
TGCCTTGGGGTCCACATAATTGTTGTACAAACCAGAGCTTTTTAAAGTGAAA270.11985085227272728No Hit
TATAGGGTGGGAGGTGAAACGGTACGATGGTCTCAACACCTTTCTTCTGAAC260.11541193181818182No Hit
GTAGAGGGGTGTTTGACACAGGGTATCTAGGAGGGGCGGGGAAGACATGGAT260.11541193181818182No Hit
AGCCATGGTGGACATAGCTAACTCCACTAGAATCCTAGCTGGGGGTGTTGGA260.11541193181818182No Hit
GTGACAGGGAAACCTGGATATGTACCCCATGAATCTAAAATTTAAAAAATAA240.1065340909090909No Hit
TCTAATGGGGCAGGATGTGCATTAGCAAATTGCACTGTACTTTTCACTCCAG240.1065340909090909No Hit
GATTTTGGGGTACATTTTGATTGCCAGTATCAGTTTGGAAAAATGTTTAAAC240.1065340909090909No Hit
TTAAAGGGGCACTTTTGAAGTGGAATTAGTGGATATTGATTGAAATAGCAGT240.1065340909090909No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGACTGTCTCTTA240.1065340909090909No Hit
AGATAAGGGCCACAAGGACTGAACACCCCTACTCCAATTTAGACCTGCGGAT230.10209517045454546No Hit
AGCACTGGGCACTTCTCACAAACTTTCTAGTGAACAAAAGGTGCCTATTCTT230.10209517045454546No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)