FastQCFastQC Report
Sat 31 Dec 2022
SRR5845311.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845311.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22371
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATAAGGGAGCTCTCCGGAAGCTAGAAGCAGATGATTTTTTCCAAAGCTGC5142.297617451164454No Hit
GATTGTGGGCTGATAATGAATGCCAGCTTTCCTTGTGCTTTGATAACAAAGA4662.0830539537794466No Hit
TATTCTGGGAGAATACATCCCAGTAAGGGTCCTATGGAGAAAGACCTGTTGT4361.948951767913817No Hit
TGCAGTGGGGGGACAGAACCGTGTCCTCAGGAGCTAACACCTGGGCTCCATC4231.8908408207053775No Hit
TATGTAGGGCGGCGCCGACCAGGAAATTTCGATTTGCACACTGATTTGGCCC4021.7969692905994368No Hit
ACTTTAGGGTAAAATTTAAACAGATGGGCCTGAATCCAAACAAGGACACAAC3631.622636448974118No Hit
TAGCTGGGATTACAGGTGTTTGCCACCATGCCCAGCTAATTTTTGCATTTTT3331.4885342631084886No Hit
GGTGCTGGGATACCCAGAAAATTTCATACTACTGTTTTCCAAAGTGAAGTTG3251.4527736802109874No Hit
CTCTCTGGGCTTGCAACCTAGTTCATGATAGCAGAGTACATGGGCATGCAGA2991.3365517857941085No Hit
GTGACTGGGTGAGGGCTGGCTATTGAGAACTGGAAAGAGTAAAATGATAACT2981.332081712931921No Hit
AAGCCAGGGCTCGGGCATGGGGGTCGATAATAATTAAGTCTTAATTAGTAAC2821.2605605471369183No Hit
TTATGAGGGTACAAATGAGCAAGGTATTTGTTGCAACTTACTACATAGCATA2771.2382101828259802No Hit
AAATAGGGGGAGTCATCCTGAACTAGTTTGAAATTGTGAAGCTGCTTCTTGG2741.2247999642394172No Hit
TTAGGAGGGGTGTAAAGGTGAACACTGTCACCTCAACCTGTTCCAGGTAAAC2721.2158598185150418No Hit
AATCGTGGGATTTATGAATAACTGTTGATTTGTAAAGTCATTATGGACTTGG2631.1756291627553528No Hit
CTTAAGGGGGTTAAATTTCTTGTACAAAATTGTTTATTTGCCTTTTCTTTGT2471.1041079969603504No Hit
TCTTGTGGGAATGCCAAATTATTCTTCATAAGAGCTGTTGCCAAATCAGTGA2401.0728174869250369No Hit
CAAAGGGGGGAAGAAGACACATTTCTAGATTATTTGTCCTTTTTATCCTCTC2361.0549371954762863No Hit
GTAGCCGGGGAATTGATTGTTGTGTAGGTCTAAGATAAGGGTCTGATTTCAT2351.0504671226140987No Hit
TCTTGAGGGAACCGGCCTCCCATAAATTCCAATCACATTTCTTCCCTGCTTA2281.0191766125787851No Hit
ATCGCGGGGGGAACTAAGCAGTGAACACACTGGGGGAAACGTTTGCTCCAGA2140.9565955925081578No Hit
CACAGCGGGAAGTGTTTTATTAATAATTAAATGTGATAATACTTAATGAGAA2090.9342452281972197No Hit
ACGCAGGGGGATACCAAACCCTTATAAATCAAACTCACAAGTTAAAAAAATT2030.9074247910240937No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT1990.889544499575343Illumina PCR Primer Index 5 (95% over 21bp)
ACTTGGGGGAAGAAATTGGCAATATCGTATAAAAATCTGCTCATATTAGAAC1980.8850744267131554No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC1980.8850744267131554No Hit
CAGACTGGGATTCATAAGTCATAGAAAATAAATACATCATATAGTTGTATGG1840.8224934066425283No Hit
ATCACCGGGGCACCACTGCCCTCCAGCCTAGGTGACAGAATGAGACTCTATC1670.7465021679853382No Hit
GTGATGGGGAAGACAATGGAGGGTTTTTTGTTTGTTTGTTTTTACTATAACT1630.7286218765365875No Hit
GTACATGGGGAAAGTGGCAGTCCCAGGACCTACCAGGTAAAGAGATCAGGAC1540.6883912207768986No Hit
CTGGCTGGGATTACAGGTGCGTGCCACCACGCCTGGCTAATTTTTGTATTTT1400.6258102007062715No Hit
GCACAAGGGAAGGGGATTGAATCCATAATCATAAATCTGTCAACAGAGAAGA1340.5989897635331456No Hit
GATCGCGGGATTCTAATCTATGCTTGTGATACAGATTTCTTTATATCTTCTT1340.5989897635331456No Hit
ACGTTAGGGCAGACATCACATGTTCTGACTTATTTATTGGAGCTAAAGATGA1330.594519690670958No Hit
CTGGCTGGAAGTCCTGATACTGAATCTCCAGTGTTAGTGAATGACTATGAAG1310.5855795449465826No Hit
ACTGAAGGGTGGGGACAATGTGATCATCCTTGGAGAGAGATGTGAGCACCAA1310.5855795449465826No Hit
TCAGGCGGGATCTGCCAGTGCCTTGGTTTATAGGCCATTTAGTTTATGATAT1260.5632291806356443No Hit
CAAGCCGGGAAAGGAAAGAAAGCAGAATCCAAAACATCAGAATGAATTGAGA1250.5587591077734567No Hit
TCAGGTGGGACCCCCAGGCGAGCATCTGACAGCCTGGGTCGGCACCCACACC1240.554289034911269No Hit
TCTAGAGGGTCTGGAATTTCAGTAACAATATGAAATTAACATCATTATCATT1210.5408788163247061No Hit
ACAACGGGATGGTTAGTATTGGCTAAGAAAGACTATAAGGTATACATGTTAT1170.5229985248759556No Hit
GGCCAGGGTGGGCGGATTACCTCGGGTCAGGAGTTCGAGACCAGCCTGGTCA1140.5095883062893926No Hit
GCAAGGGGGCGATCCTATCATGACTTAACATCAAGCGCGCAGTTCAAAACTA1100.4917080148406419No Hit
AACGCCGGGAGGACAGATGGAGCCTCCCTTTCCTCATCTACTCTGTGGGTGG1090.48723794197845427No Hit
TGAATGGGCACCAATCACCTCAGCCCTCTACCCTACAGATTTCTAAATCATA1080.4827678691162666No Hit
CTTCTCGGGAAGGAAGGAGTGGTTAGTAAACTGCACTGTTTCTCTGATAATG1020.4559474319431407No Hit
CGCAGTGGGGCCCACTGGCTGCTCTGAAAAGCCATCTTTGCATTGTTCCTCA1010.45147735908095304No Hit
ACGACAGGGCTGACATTAAAATTGTCTGATTTTGTCCGTTTCTTAAAATTAT980.43806714049439005No Hit
CACTCCGGGATTGTATATGAGGTTGCAGTGGCTTAAAAAGGAAGGACTTTTG970.4335970676322024No Hit
GGGCCTGGGAGGCGGAGGTTGCAGTGAGCTGAGATTACACCACTGCACTCCT880.39336641187251353No Hit
TATTATGGGGGGAGTTAGAGGCTGTTCCAACCATCCTTAGTTTGGGAGAGTG850.3799561932859506No Hit
CATAAAGGGCAAGTAGTACCATTTTAAATGTTATTGCAATTTGGAATCTGAA810.36207590183719995No Hit
TAGCAGGGGCTGGGAGGCCGAGCTTGCAGTGAGCTGAGATCGCGCCACTGCA800.35760582897501225No Hit
GACAGAGGGTTCTGTATACATGTAGCTTTGCCAGATATGTACTTAGTAATAT750.335255464664074No Hit
TCCTACGGGAAGAACTGAAATTTTTAATTAAAATTTATTTCCCCTATTAGGC730.32631531893969873No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTG680.30396495462876044No Hit
GGAGCTAGAAATACCATTTGACCCAGCCATCCCATTACTGGGTATATACCCA660.2950248089043851No Hit
AATAATGGGGATCACCAAAGAGGAAGCCTCTGGAAGTTCTGTCACAGCTGAG610.27267444459344686No Hit
GCTCCCGGGTTCAGCTGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACT590.26373429886907157No Hit
AGTTAGGGGGAAAAAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTAT570.2547941531446963No Hit
TTAGCTGGGATACATGTGTGTATATATGTGTATATATACATACACTTGTATA560.25032408028250863No Hit
AGAGATGGGCAGGCCAAGAGTTTGAGATTAGCCCAGGCAACATGGCAAGACC550.24585400742032096No Hit
AAATGGGGGGGAGAAGGGAAAGAAATAAAAGAAGAATACATAGTATATTGAA540.2413839345581333No Hit
TGGGCAGGGAAAAGGGTCAGGGTTCATCAGGTGAACTCAACACTGGGATGAG510.22797371597157035No Hit
AATATGGGGATGGGAGTAGAAAACAACAAAGAATGGAATGGACTCTTAAAAC490.21903357024719503No Hit
CAGTCAGGGATACCATAGTTTATTCAACCAATTGCACATAATGTGGATCTCT470.2100934245228197No Hit
TTTACAGGGATATCATAGATATGAAGACATAAAGCAGCAGGTTTGGGGTTTT460.2056233516606321No Hit
TTTACTGGGGGGTGATGATTAGAGGAAAGGGTTTTTCTGGTTTGTATGTTGG450.2011532787984444No Hit
TCCCACGGGCTCTACTAAACATACAAAAAATTAGCCAGGTGTGGTGGCACCC430.1922131330740691No Hit
TGTTACGGGAGTGTATGGGATATTTATAAATTCAAAGAATCTTTTTTTTATT420.18774306021188145No Hit
GCTAGAGGGGGGGTGATACAAATGTTCTGATAGATAGAGGTGGTGGCTGCAT410.1832729873496938No Hit
AAGTAGGGGACATCCAGATGGCCTGAAGCAAATGAAGATCCACAAAAGAAGT400.17880291448750613No Hit
ATGCGGGGGGATTCGTGGGCATGATCCTGATTCTCATCTTCGCCGAGGTGCT380.16986276876313083No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCT380.16986276876313083No Hit
GAGCAGGGGATTTGTTTCCTTTGGATGCCGTATCAAATTACCACAAGCTGAG380.16986276876313083No Hit
GAATAGGGGTTAGCAAGTGAACTTTATAATATTTTAGACAGCTTATATTTAC350.15645255017656787No Hit
TATAGGGTGGGAGGTGAAACGGTACGATGGTCTCAACACCTTTCTTCTGAAC340.15198247731438022No Hit
AGTCTCAGGCTTCAACATCGAATACGCCGCAGGCCCCTTCGCCCTATTCTTC340.15198247731438022No Hit
CTTCATGGGATAGAGATAGAGAATAAAACAGTAATTACTAAGGTCAGGATAA330.14751240445219255No Hit
ATATAAGGGGGGTTCTTTGTATTGGCACTTTGCACCAGAAACATATCATTAT320.1430423315900049No Hit
TGCCTTGGGGTCCACATAATTGTTGTACAAACCAGAGCTTTTTAAAGTGAAA320.1430423315900049No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGA320.1430423315900049No Hit
ATAGAGGGGGCCAAACAGATTCACTACTCTTACTGATTCTTCACTGAGCTTT320.1430423315900049No Hit
GTTTTGGGGCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCT300.1341021858656296No Hit
TTTTAAGGGGGGATAGATAAATCAAGCTAATTATACATATGTATTACCTCAC300.1341021858656296No Hit
GGCCTGACTTGGGGGAACGATGTGTCTGTATTTCATGTGGCTGTAGATCCCA290.12963211300344196No Hit
GATTTTGGGGTACATTTTGATTGCCAGTATCAGTTTGGAAAAATGTTTAAAC280.12516204014125432No Hit
ACAAAGGGGTACATATGATATTATATATTTAGAAAATCAAAGTGAATTAACT280.12516204014125432No Hit
CAGCACTTTGGGAGGCCTAGGCAGGCGGATCACCTGACGTTGGGAGTTCGAG280.12516204014125432No Hit
GTGACAGGGAAACCTGGATATGTACCCCATGAATCTAAAATTTAAAAAATAA270.12069196727906666No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCTTATAC270.12069196727906666No Hit
TGAGGCGGGAAATCAATAAAGTGCATTTTCAAAAGAGCTACAGCAAATTTAT270.12069196727906666No Hit
AATGCAGGGAATGCAGGAGGACAGTGAAGCAAAGTCAATAGATTTTGTAGAA250.11175182155469135No Hit
AAGCAATGGGAGGGAGAGGGAGGAGGGGAAGATCTGGGCAATTTTGGCCTTG250.11175182155469135No Hit
AACCTGGGATAGTGCAAAATGAAGATTTCAAAAACCCAGTCAAATATTTTAG250.11175182155469135No Hit
GTAGAGGGGTGTTTGACACAGGGTATCTAGGAGGGGCGGGGAAGACATGGAT240.1072817486925037No Hit
CAGAATGGGGGCTTCGGGACACAGCCCTCCTGGAGGACCTGGGAAAGGCCAG240.1072817486925037No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGAT240.1072817486925037No Hit
ATCAAGGGATAGCTGGAATTAAGTGTTGTCTTGGAGCTGTTGTACATTTAAG240.1072817486925037No Hit
TGTAATGGGGTTGCAGTGAGCTGAGATTGCACCACTGCACTGCAGCCTGGGC230.10281167583031604No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)