FastQCFastQC Report
Sat 31 Dec 2022
SRR5845305.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845305.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18946
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC1120.5911538055526232No Hit
GTTACAGGGTGGCCAGTAGGTGGTAGCCACAGAGAAGCAGTGCGTACAAACA1110.5858756465744748No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT1080.5700411696400296Illumina PCR Primer Index 5 (95% over 21bp)
AAGCCAGGGATCTTGCAAAAATGGTTCTGTGCACTTGGATGTGAAATGCTGT1020.538372215771139No Hit
ATCATCGGGAATGAAACTATCTACAATTCTTGTTTTAGCACATCTGTTATCC960.5067032619022485No Hit
TATGCTGGGACTGGGCAACTATCTCTAAATGCCAGGGGGCAGAGCGTTGTGA950.5014251029241001No Hit
TATCCTGGGATGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCT930.49086878496780323No Hit
GTATAGGGTAGGGAAAAGATGCACTAGAAAAACAGTACAAGTAATGACCACA930.49086878496780323No Hit
GCAGCGGGGTGAAGTGATTCAGTTTCCTCTACGGATGAGAGACTGGCTCAAG930.49086878496780323No Hit
ATTAATGGGACTGCATATGTTCTAGTGGAAGAGAAGCCGGGGTTCTAGGAAA910.48031246701150637No Hit
GTATCTGGGACTCTTTACCCACAACTTAATCGACTCAGAAAATTCACACTGG900.475034308033358No Hit
CCATAGGGATCACCTGCCTATTTTACTTGCATTTAAAATGTTTGGTATTTTG890.46975614905520957No Hit
TACAGATGGGATTTGAGGTCAAATTGAAGACGGAAACCGGAAACCGTTTTCT880.4644779900770611No Hit
CGATCTGGGATTCTAAAATAGGTTGTCTTCAACTTATGAGCATTTTATTTAT810.42753087723002214No Hit
TAGCCAGGGGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGG810.42753087723002214No Hit
AGAACGGGGTCCACGTTAGTTATGGGAATCCAGCAAGAAAACAGACAAATCA800.4222527182518737No Hit
TCTACCGGGTGTGCATGTGTGCGCGTATTACGCTTGCTAAAATTTGTTCTGA770.4064182413174285No Hit
GGATGTGGGACGGCTACATCCAGAAGATCAAGTCGGGAGAGGAGGACTTTGA770.4064182413174285No Hit
GGACCTGGGAGGAGTCCCAAAGCAAATGTCCATGTCTAAGATGAATATTTAA760.40114008233928No Hit
ATTAGTGGGCCAGGATTGCCTTCTGTGTGACTTTTTCTTGCAGGATCTGACA740.3905837643829832No Hit
GGAGTTGGGAGAGTTCTTTGGAAAGAGTGGGGCAGTTGCTGAAAAGGCAACA710.3747492874485379No Hit
ACTTAAGGGCTACATCAAAGAGCTAGGAAAAGCCATTGTCAAGAGGGAAGGA710.3747492874485379No Hit
AATGCTGGGGTTGGCATTTAAATTATTTGCTGTTTCTGGCTCAGATTTCAAC710.3747492874485379No Hit
TGCCAAGGGATCTCGCTGGAACAGGTCTTCCTGAGCTTCGCCCACCTGCAGC690.3641929694922411No Hit
ATGGATGGGAATGTCACTTTCTGGGTATACAACTTAATCAATCCAAGGCACC690.3641929694922411No Hit
CATATTGGGATGGAGCCCGAGGAGGAGCCAAGTGTGGCAGCAGTTCGTGAAG680.3589148105140927No Hit
GTCAACGGGATGAGAAAGGTAATATTGGTAAATAAAGAAATAGGAAACAATG680.3589148105140927No Hit
CAAAAAGGGTGTCCCAGGGATAGTACCGCAAGGGAAAGATGAAAAATTATAA680.3589148105140927No Hit
ACAATCGGGACTTCTGAGCTTGCAACATAGCTCACTGAAGAGCTGTTAGATC670.35363665153594426No Hit
CTTGAACGGGGCTTTCCGCTCGGCTGTTTTCCTGCGCAGGAGCCGCAGGGCC660.34835849255779583No Hit
CAATGGCGGGCAAAAGGACGACGGTGGCCAACTAAAGCAAGTCTTCCTTCCA660.34835849255779583No Hit
ATGCTAGGGAGAAATCTGAGAATGAAGATGACAGCGAGTGGGAAGATGTGGA660.34835849255779583No Hit
GTTAAAGGGAACCTCCTACTTTTATAGACTTGAATACTTAAGTAATTTAAAT650.3430803335796474No Hit
CCACCCACGGAAGGACTTGAGTTGTTTACAAGCCCTGCACTGAGGCAGATTG650.3430803335796474No Hit
ACTGCAGGGGGGAGCCGGCCGCGATGAGCGGGGAGCCGGGGCAGACGTCCGT640.337802174601499No Hit
ACTAGTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC640.337802174601499No Hit
AGACTTGGGAGGTGTACATGTGCATGAGTTGTGTACATGCGTGAGCACGTGT640.337802174601499No Hit
AGACTGGGGATAGGTGATAGTGATAATTCCTGATTCCAAGAATGCCATCTGA630.3325240156233506No Hit
ACTTCAGGGAAACATCGGTCAGATGAGTCATACATTGGGTTATTTTTTATAT630.3325240156233506No Hit
GCGCAAGGGCTCTGAGAGAATGTGCTTTGGAAGATAGATAATATAATTATGT610.32196769766705374No Hit
TACGTAGGGGCTTTTGATGAGAGTGCCCTAACCCCAGACAAGCTCCTTGTTC610.32196769766705374No Hit
CGCAATGGGAGATGAGGGCTATGGCATTTCCTCTGTGCCATCTGCTTCCAAT600.3166895386889053No Hit
CGATTGGGGCACTTTTGGAGTCTCCAAATCCCTTGAGAACCCATCTCCCGGT600.3166895386889053No Hit
GTAATAGGGACAACCTAGGTGTTCATCAATGGGCAAATGGATAAAGAAACTG600.3166895386889053No Hit
GTCCTTGGGATATCGCTCACACCTCATATCCTCCCTACTATGCCTAGAAGGA590.3114113797107569No Hit
GGACCAGGGAGATTTACTGGCAACACATTTTCTCAATTTTTGTTTGTCAGAG590.3114113797107569No Hit
TTGCATCGGGGATGGGGCAAATAGCATATATGAGCTAAACCAGTTAACTGTT580.30613322073260846No Hit
GCCTTGGGGGGTCACACCCGGAAGCAGGGGCCCGAGCGGAGCCGGCCGCGAT580.30613322073260846No Hit
TCGCTAGGGAATACTTCCAAATATAGTAGTTACCTTTTGAACTTTTCTATAG570.30085506175446003No Hit
CAACTAGGGTCTGGGATGGGGGTTTGTGAAACCAATTTACTGTAGTTCTGTT570.30085506175446003No Hit
TCTACAGGGTCCTGCCATTGTAACATCATAACTTAAAACACTTTTTTGGAAG570.30085506175446003No Hit
TCTAAGGGGGACATTTCAAAAGTGAACTTGATTCAACCTGAGAAAACTGATT570.30085506175446003No Hit
TTTAAAGGGGAGAAAGAGGTCTTCTAGCCACCTGGGCTGCTACTGAATGGTT560.2955769027763116No Hit
GTGGTTGGGGACAGTTAGAAGGTTATCACCTTGGTGGCAGACTCCAGAGTGG560.2955769027763116No Hit
GCAAATGGGTAGTCACCCATAAGCATGGTGAGCTCCTGCTCTCATAAGGTCA550.2902987437981632No Hit
GGGGCAGGGGGAGTTGTAAGAAGGTGCACATATTTTTTTAAATCTCACTGGC550.2902987437981632No Hit
GTTAGTGGGATGTGGAACCTCTGAAATTATACCTTCAGAAATTCAGAGAGGC540.2850205848200148No Hit
GGATTTGGGACTGGTTGCCTAATTTTATGGATGTCTTCACGTGGTCTTTACC540.2850205848200148No Hit
TGTACCGGGATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATA540.2850205848200148No Hit
ATTTTGGGGGACCTGGAGTGCAATGAGAATGTGAAACACAAAACCAAGGAGT540.2850205848200148No Hit
TTTCAGGGGACTGTCACGTGAGCTGGACCCACCTTGCTCCTGGTCTGCGGCA530.2797424258418663No Hit
TCGCTGGGATTGGCACACACCTGTGGTCCCGGTAAACAGGGGGCTGACGTAG530.2797424258418663No Hit
TTTCGCAGGGCATAGGAGGCTGGTGGCAGCTGTATTCATTATTGTCTAGGTT530.2797424258418663No Hit
CTTAGAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC510.2691861078855695No Hit
TGTTCAGGGATGCTAAGCACTTTGGGAGCAAGAACCTCCTACCTTCCAGTTT510.2691861078855695No Hit
CATAGTGGGAGTCATGCCAAATGATACCCTCTCAGCCCAGTAGTTATTTATA510.2691861078855695No Hit
TGATCAGGGAGGACTATGATAAATATCATTGTATGTATTAACAGTGTCTGTC510.2691861078855695No Hit
ACATCTGGGAGACTAACTTCTTACAGCAGAGATTAGTTTGGCTTGTTTTTAA510.2691861078855695No Hit
TTTTAAGGGTGGCTTACTTGATTATCTAAAGGGAACTTTTACCATCTCAAGA500.2639079489074211No Hit
TATTTGGGGAAATTGCTACAGTTGCCATCACCTTTCTGTGGTAATACTACTG500.2639079489074211No Hit
ACCACCGGGAGAGACAGCTGCCTGCTGATAGCCCAGGAACTCCATTCTTGGA490.25862978992927266No Hit
TGTGCTGGGGGTTTGCCGCTGGAGTATCAAGAGAAGTTAAAAGCAATAGAAC490.25862978992927266No Hit
TGCCGTCGGGATAATGGGATCAATTATTATATTATAACCATACTTAACAGAA490.25862978992927266No Hit
AGCTATGGGTCCGATCTGAAAGAGGGTCTGAGCTTGATCCATTTTCGCTTAC490.25862978992927266No Hit
TCGCACGGGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGA480.25335163095112423No Hit
GTACCAGGGAAGTGGTAACAGGACTGATGCCGAAATGTCACCAGGTCCTTTC480.25335163095112423No Hit
GCTGTCGGGGATCTGTGATGGGGATTCCACTGACTAACTTTGGTGTTTTGTT480.25335163095112423No Hit
CCAACAGGGAGTAGCACAGAGGAAGAGGCAATACTACCCGAGACTAGATGCG470.2480734719729758No Hit
ACATGGGGGCGGTGCAGCGGTGGAAGGAGCCTGCGGCTGCTGGCACAGACTT460.2427953129948274No Hit
GAAAGCGGGGTTTGGTCTTTGCCAACGTGTGTAGAGCCAGTTTTATAGGAGG450.237517154016679No Hit
TCGTCAGGGCGTCCAGCACCATGGTGCCGAGCCCAGCAGCTTGGACAAGAGG440.23223899503853054No Hit
TTAAACGGGAAGGACATCCAGAGGAGACCTCACTTTTCATTAGACTGCTTTT440.23223899503853054No Hit
TGTGGTGGGCGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC430.22696083606038214No Hit
ACGACGGGGGTGGCTCTCAAGGAAAAGCTCCATCTGCTTTCACATATGCAGC430.22696083606038214No Hit
GGCCTGGGGGAAACAAAATTCAGCAGAAGCAAACCAAAAAAATAAACAAGCT420.22168267708223371No Hit
TAGTTTGGGCAGGAAATGTCCTGACTCCCCTCAGCTCCCCCTTCACCCCTCC420.22168267708223371No Hit
TTTGACGGGGGAACAATGAAACCAACAACAAACATTTTTAAAATTAAAATGG420.22168267708223371No Hit
AAAATTGGGCTCCTAACGGGGGCTGGGGGTAAGAGAAATCTAACTGCGCTCC420.22168267708223371No Hit
GTTAATGGGGGGCAGTAACTTTTGAGAGAGGCCAAAAAAAGGAGGATGACAT410.21640451810408529No Hit
TACATTGGGATGGTGTGAAATATTGTGTGAATATCTAGGCAAAACATAACAA410.21640451810408529No Hit
GATGCTGGGAATTGAATTGTATTATAGACATTATGAGGTAATTGATCTTTTA410.21640451810408529No Hit
GAAACTGGGAGTGGGTCATACTCTGTCCGTGATGTCTTCCAAGTTCAGAAAG400.21112635912593686No Hit
CTTACCGGGGTGTTCTAAGCCCGAAGATCCAGTAGAATACTTGGAAAGTTGT390.20584820014778846No Hit
GCACTGGGCACTTTGGGAGGCCGAGGCGGGCGGATCACGAGGTCAAGAGATT390.20584820014778846No Hit
AGCCTTGGGTCCAAGATGCTGGAACAAATAATATTTATGTCTAATTTTGTGA370.1952918821914916No Hit
CGCTATGGGAACAGTGGCAAATGTTTTCATTTACTTTGAATTTGAAAATGTT370.1952918821914916No Hit
TATAACGGGAACTTATACTGTAGGTGTTATGTATTCCTATGACAAAAAAAAT360.1900137232133432No Hit
GCCTTTGGGCTTGCCCTTGCCACACTTGATCTCAAGGGCATCTTGGAAGTAT360.1900137232133432No Hit
TGTTTCGGGTGCCCGTGAAGAGGCGGGCATAACACAGCAAGACGAGAAGACC360.1900137232133432No Hit
ACCGCTGGGGTCTGTGATTTTAAACTAACTGTGTATGTATTGATGTTTGGAA360.1900137232133432No Hit
GTTATAGGGACAATAATCCTTCTACCTCTCACTTTTAACATGGGCTATGATA350.18473556423519477No Hit
TTGTCAGGGAAGGCCAGCCACACCACAGGATCTGAACTTTGTTTCCAGAAAT350.18473556423519477No Hit
ACATACGGGTATTGTGAGTTAGGATAATAACTTTTTTTTTTTTGTGCTTCAG350.18473556423519477No Hit
CAATTTGGGATGTTTTTGGAAGAAATTTTTACAGAAGACTTTCATTATAGGG350.18473556423519477No Hit
AACTGTGGGTAACTGAAATATGATGGTATTTACATTAATTTTTGAAATTCAA350.18473556423519477No Hit
AGTCATGGGAGATCTCTTGGGCAGAATCCCACAGAAGCAGAGTTACAGGACA340.17945740525704634No Hit
GTGATTGGGGACCACCAACCCACAGGAGCTGATGCTGCAGATGAACCTGCTG340.17945740525704634No Hit
CCAGAGGGTTACCATACTGAGTATCTGTTTTTCCTCAGGCACATCATTTTTA340.17945740525704634No Hit
GTAACCGGGGATGGCAGACGCCTGGTGTCAGGAGGGGCCGCCACCAAGGAGC340.17945740525704634No Hit
CATGTAGGGCCAAGTGTGACCCTTAGATGCTTAGTTGACTCGCTGCATATTT330.17417924627889791No Hit
TTTAGCGGGATGAATGTGGTGATTTCCCAGATACCATCTCAGGCTTAACCTA320.1689010873007495No Hit
GTATCTGGGGCCCCGCACCATGCCGTCGGAGAAGACCTTCAAGCAGCGCCGC320.1689010873007495No Hit
AGGTCTGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA320.1689010873007495No Hit
AATGCAGGGCTTTTAAGTCCCCTGCAATCCTTTCTGGAACTTAGCAAAGTGA320.1689010873007495No Hit
TTCATAGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATT310.16362292832260106No Hit
AAATTAGGGTGCCACAGAAGAGCAAAATTGTATCCAAATTTATGCCACTTAA310.16362292832260106No Hit
AAAATAGGGGTTCAATGGAAATAGTGATCTTTCTCCATTAATTTACAGAAGT300.15834476934445266No Hit
TCTTAAGGGACAACCATAAACAGAGATGGGAAACTGATCATAGGTTTTGCAT290.15306661036630423No Hit
ATAGCGGGGGATGAAATTCTCTAATTATTTCTAGGGAATGTGTAGAAATTAA290.15306661036630423No Hit
CTTTTCGGGAATTGTCTAGAATGTAAAAAGTCTTTAAGACATAAGAATTCCT280.1477884513881558No Hit
CGATTAGGGTCTGAGATAGGGGATAGTGGGAAGGGGACGCGCGCCCTGCGGC280.1477884513881558No Hit
ACCGGTGGGAGGCCACATACAGTCTCTGTCATAACTACTCAACTCTGTTTCT280.1477884513881558No Hit
TTTGCAGGGAACCTAAAGGAATATGCCTGTCGAGGAGAATGAATGTGTATAA280.1477884513881558No Hit
CCAAGTGGGAGGATATTGCCCTGCCATCCATGAAAACTGTTCTGGCACCAAA270.1425102924100074No Hit
GGCAATGGGATCCAGCAGGAGAAAGAGTGTGTTCAAACATCATAAAATGGGG270.1425102924100074No Hit
GTGTGTGGGGATAATTCACATTGCATAAGGTGAAAATTGGGAATTTGTTAGG270.1425102924100074No Hit
GCTTTAGGGGAGATTTCATATATTAGTAAGTTTTCTAACATCGGTTTAAGAA270.1425102924100074No Hit
ATAGGGGTAACTCATTGATCCAATAATGTGTCTAGATTTTCTCAAAGTCTTT270.1425102924100074No Hit
TATCATGGGGCAGTATGCAGGTCCAGCTGCCTACCCTGCTGCCTATGGTCAG270.1425102924100074No Hit
AAAGAGGGGAGGAGAAAGCTGACCGCTTAGGCCGGGGTAGTGGTCGTCGTGG260.13723213343185897No Hit
AATCAAGGGCATAAGAAGCTCTTCCCCTAAAGGCAGCACAGCTAACTCTACT260.13723213343185897No Hit
ATAGGCGGGAGTGCGCTGCAGGCAAAGCCCAGGCCACGGCTGCGTCACTACT260.13723213343185897No Hit
TCGCCAAGGGGGATAGAGTGAGTCTCTAGCTCAAAACTCTGTGTAGTCAGCC260.13723213343185897No Hit
CCATCAGGGAGATCCTGTCTTTGTAAAAAAACTAACCAAACAGGAAAAACTG260.13723213343185897No Hit
TTACTGGGGCACCACCTCACCTGGCCAGAATGAGCTATTATACGACCATGAT250.13195397445371054No Hit
GCTGGCCGGACAGTCACCCTCTGTTCTCGCTACATCCCTTGCCCCCTGTCCA250.13195397445371054No Hit
TATACCGGGATTGAGAAACTTGCCAAGAACAAACAGAAGCCTATCACTCCAG250.13195397445371054No Hit
CACATCGGGGGGGAACCTCAATTATTTGAGTGACTCCTATGTTTTCATGAAA240.12667581547556211No Hit
GCACCTGGGACTGTTCAAAATGCTGACACAGAAACGCAAAGTGAGCACATGC240.12667581547556211No Hit
CCCTTTGGGTCTCAAAGCTGATGCCCCAAATGGTGTTACTTAAGTTTACTTC240.12667581547556211No Hit
TGTGCTGGGATAGTCAATATACAGTCAAGGACACTGTTCTGTCAGTAATCAG240.12667581547556211No Hit
GCTGGGGATGCATAGCTGCAGAAACCATGATAGGTAGAGGACTTTTCTTTTG240.12667581547556211No Hit
GTATTAGGGGCCAGCTCAGAGTTTAGGTGTACACATTTACCCAGTTGAGCGT230.1213976564974137No Hit
TTACTGGGGGCCTCAAATGCTAGCACTTTTTGGCAGTTGGATAGGAAATGAG230.1213976564974137No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCTTATAC230.1213976564974137No Hit
CATTTAGGGTCGGTAGTCATATAGTAACATTTTGCTATATGAAAACTTTGGT230.1213976564974137No Hit
TATGCTTGGGGAAGAGTTTTCCACTGTATACATTTTTATCCAGATGAAGGTA230.1213976564974137No Hit
CAAGCTGGGAAGTTATTAAACATATTTTAATTATATTCATCCCCAAACTGCA230.1213976564974137No Hit
CTCATAGGGAGTCCATTCTTCAGTCTAAGCAGTTTGAATCACTTGCTGTTTC230.1213976564974137No Hit
GTTTTAGGGACCCTGGAGAGTGGTGGCCACTCAATACCTTTTCCTCTAGCTT230.1213976564974137No Hit
TGCCCTAGGGACTTTGGGAGGCCAGGAGTTTTGAGACCAGCCTGGGAAACAT220.11611949751926527No Hit
CAGAGTGGGGGAGTGCTCATTAGGTCGCCGGGCGTCCACGTGCAGCCCTGGA220.11611949751926527No Hit
CATCGGGGGAGCGGCAAGCCCCTCTTTTCAGAGTGGGCAGAGGGTTGCCTAT220.11611949751926527No Hit
CATCTAGGGAGTTTCAGTTGCCTCATTCTATTACATCATTCTTGTCAAGTCT210.11084133854111686No Hit
AGCGGTGGGGCGCCCGACCTTTTTCACTTCTCTTAATGCTCTGTAAACATTA210.11084133854111686No Hit
GTAGCCGGGGATGCTATTGTTCATTTCTCTACTAGTAAAATGATCAAGCCTT210.11084133854111686No Hit
GCAACGGGGATAACAAATTGTTCATAGTAAATAATCACCACCGTATGGATTT210.11084133854111686No Hit
GAATCAGGGGTTTACTATCCATGGCCAATCAAGGCCAGAATACCAATAATTC200.10556317956296843No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCT200.10556317956296843No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGA200.10556317956296843No Hit
TCAGATGGGAGTGACGAGGAACTCCCGAGACGTGTAATGACACCACACTTGT200.10556317956296843No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTG200.10556317956296843No Hit
TCGTAGGGCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCAA200.10556317956296843No Hit
ACCATGGGGCGGGTCCAGAGACCACACCACTAACCATCCCCAACTCCATGGG190.10028502058482No Hit
AAACAAGGCTTCTTGCGGCACCGCCCACCCACACCCCGTCTGCCCCTTGACC190.10028502058482No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)