FastQCFastQC Report
Sat 31 Dec 2022
SRR5845303.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845303.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32855
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC2460.7487444833358697No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT2270.6909146248668391Illumina PCR Primer Index 5 (95% over 21bp)
CTTATTGGGAACAACAGCTGGTGCCCTGCTTTCAGATACTGGATTGTACTTT1250.3804595951909907No Hit
GCTGTGGGTCTAACAGGTTACAGTACATTTCCTCTGTATGTAAATTAGATGG1210.36828488814487903No Hit
TAACATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1100.33480444376807184No Hit
AAACAGGGGTCTGGTAAGGGGACAAATAATTTGAGTATCAGAATGAAACCTT1090.3317607670065439No Hit
TACGTCGGGGAGAGCGCGCTCGGACCTCCTTCCCGGCGGCAGCTACCGAGAG1080.32871709024501594No Hit
TCGTATGGGCCAGGATCCAGTGACTTGTCCTCCAACAAAACCCCTTGATCAA1070.32567341348348805No Hit
TAAGCAGGGCCCTTTCCGGCCGGTCCCCATGGAGGCGCTGGGGAAGCTGAAG1060.32262973672196016No Hit
TTGAGGGGTATATAGAATTCCCAAAACCAGCCCATGCCAGATTTTTTCAAGT1020.3104550296758484No Hit
TGTACAGGGCAGCAGCTGTTCAACAAGAGTAAATATTGTCACAACACTGAAC960.29219296910668086No Hit
ATTAATTGGGGAAGTCTCTTTTTTGAAACACTTCAGAACTGCTGCTATAAAG940.286105615583625No Hit
TTACCTGGGCAATCACATGTAAGGTTTTGACTAAACTAACGGATAAAGATTT920.2800182620605692No Hit
AACTGGGGGAAGATGTATTTGCTAGATTGATGTGCATGTTCAGTTCTAAACC920.2800182620605692No Hit
TACAAGGGGGTCTAGACGAGGGTACCCAAACACTGGAATTGTTTGAATGTGT890.2708872317759854No Hit
AAGTCTGGGCATCTAAATGAGTTTTACTAGCCAGGAACCTAAAAACACCCTT890.2708872317759854No Hit
GAAATAGGGAGCAAGCTCCTATGTGTATATTTTCCTGAATTTCTCATCTCAA880.26784355501445745No Hit
TGCACCGGGAATTAAAAATTAGCTGGGCATAGTGGTGTGTGCCTGTGTTCCT850.2587125247298737No Hit
TCCATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA810.24653781768376198No Hit
TCAACCGGGAGCAAAAGGAAAGAGAGGTTCTGATTCTTACCTCAGGGTTTTT810.24653781768376198No Hit
ACGGGGGCACGCATGTTCATTCATTGCTACATAATTTGGTTCTTCTGGAATA800.24349414092223404No Hit
TTTCGCGGGAGGACTGGGAAGCAAAGAAAATGGAGACCATGGGGGTGTGGGT790.24045046416070615No Hit
GGCATTGGGACAGGCTTAAGGGCATGGACTGTATTTTATTCATATCTGAATA780.23740678739917823No Hit
CTTACCTGGGTGACAGAACAAGACCTTACCTCAAAAAAGTAAATAAAATGAA780.23740678739917823No Hit
TTGCTCGGGGGGGAGCTGGGGGCATGGCGTCCGGAGCGGCTCGCTGGCTAGT770.23436311063765028No Hit
CATCTAGGGGCAAGCCAGTCATGGAATCTTGCTGCAGGCCCTCCCTCTACTC770.23436311063765028No Hit
CATAGCGGCAACATCAATTAGGGAACCAAAGTTGCACTATCTGGGCCCAGAT770.23436311063765028No Hit
AGTAATGGGATCTGCAGGAGGGGAGACCAGAAGCTGTCGGAGCTGGACGACC770.23436311063765028No Hit
CAGCATGGAAAATGCCAACCAAGAAAATGAAGAAAAGGAGCAAGTTGCTAAT760.23131943387612236No Hit
CTCGTGGAGGGGATTTTGGAATTTAGTGCCCATATGCTGACAGATGGTTTAA750.2282757571145944No Hit
CTTGCAGGGGAGCAGTCAGAGTCTTCCCTGACTCCACAATGCCCCTTTCCTT740.22523208035306652No Hit
TATAGGGGAACCACTATCACGAAAAAAACTCTACCTCTCTATACTAATCTCC740.22523208035306652No Hit
AACGCTGGGGAAAGGAGTTCACTCAAGTTCCCAAGGTATTCTGTCCTCCAGG720.21914472683001066No Hit
TTATTGGGGGCGTGTGACCCGCGGGTCGATACCTGGCAGCCCCAGTGCTGGG710.21610105006848274No Hit
AAAACTGGGGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACA700.2130573733069548No Hit
TAGCAGGGGTATTGCTTAAAAAATGTATAAAGCAGCTGGAAATGTTTTAAAT700.2130573733069548No Hit
TCCGTAGGGATTTCTCTTTGTCATGAATTGCATACTTTGTTCCAAGTCATGT700.2130573733069548No Hit
TATGGTGGGCTGGTGTAAACTGCTCACAGAAAACCCTATGAAACCAAAGGGG680.20697001978389895No Hit
GCGCTGGGACTCAGTTTTAAGACAAACACTACTTCCCATGCGGGGTACTTTC680.20697001978389895No Hit
ACGAAGGGGATTGGAGCCTCCTCTACTTTTTCTTGAGGGTTGCAAGTATTGT650.19783898949931517No Hit
GCTTTGGGACTTGTTCTTAATTAACAAAAGAACTAGAAAGAAGCTCATATGA640.19479531273778725No Hit
TTAGCAGGGGTTATGGGAAGAAGAATTTATTGAACGCTGTTTCCAAGAAATG640.19479531273778725No Hit
CTATAAGGGATAAATGAATAATTTAATTTAGTGCTCATTTGAAAAATATTTT640.19479531273778725No Hit
TTCTGAGGGGAAAAAGATGAAATTAATGAGGTCGGAGGGGCCAGATGTTTCT640.19479531273778725No Hit
TACTTTGGGGATTGCCCGACATCGCAGAGCGAGAGCAATTCCTTCTGGGACA640.19479531273778725No Hit
CAATCAGGGCAAGAAAATAACAGAGTAAAGACAAGAATATTTCTGCATGTTT630.19175163597625933No Hit
ACTTCAGGGTAGCCGCTAGCATCTTCCCGAGCACCGGGATCCCGGGGTAGGA620.18870795921473138No Hit
AGCACTGGGCCTAAAGAACATGTGTCTTGCAAATGCCATGGAACTGAATATT620.18870795921473138No Hit
ACTTGAGGGAGGCCAAGCACAGATAGCTCAGCTGCGACCTCTGGCCTCTGGG600.18262060569167554No Hit
GCCTCCCGGGCCCAAGTGATCCTCCCGTCTCAGCCTCCTGAGTGGCTGGGAT580.1765332521686197No Hit
TACAGTGGGTCCTGATAGTGGGATGAGGAAGGTCTTCCTGTTTTCACCATCT570.17348957540709176No Hit
CGCATCGGGAGATAAGCATGTGATGCAGAAGTTGATTCAAGCAAGTGAATCC530.16131486836098008No Hit
CGTGACGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA520.15827119159945213No Hit
CGCGTGGGGCACCTGGGAGGAGAAGGAATTGTGGCGGGCCTAACACCTTTTC510.1552275148379242No Hit
AGGTTCGGGGCCGAGCGCCACGGGCCGGCTGTGCGCGGGCCGGGGAAAGAGG510.1552275148379242No Hit
GTTTAGGGGATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGAT490.14914016131486835No Hit
GCCAGGGGCACCCTGGGTTGCATACTTACTACTCAGGGGCTTTCCCCCCAGC470.1430528077918125No Hit
AGAAATGGGTGTGCCAACTTGCAAAAGGAATAGAAATGTCTGTGATCTAGAT450.13696545426875667No Hit
ATTGTAGGGAGTACAGGGAATATAAAATTGGTTTCCTGTGGTCATGATCAAG440.13392177750722872No Hit
TTGCTGGGGATACAGGTAGAGATGTATGCAGATGTGTCCATATATGTCCATA440.13392177750722872No Hit
GCCAAAGGGGTATGCCCACAATGTACATTTCTCGGCATCTGTGCCTCAGTTT440.13392177750722872No Hit
AATGTTGGGACAGAAACCTAGCCATTCAAAATCTGTTTAAAGGACAAAGTTT440.13392177750722872No Hit
ATCGAGAGGGATTTTAACAATGGATCCTATAGTTAATTCTGTATAATAATTT430.1308781007457008No Hit
CAGGCGGGGTGATTTAAGAAGTGGCTGGATTGGAACTTTTAATATGCTAATG430.1308781007457008No Hit
TTTAGCTGGGCTGGAACGTCGAAATTGTCTTCTTTGGAAAGAACCATCCCCT430.1308781007457008No Hit
TTGGCTGGGGTCCCAGTATACATATTTGAATGGTTGATGGTTTTCAAGACCT420.12783442398417288No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTG420.12783442398417288No Hit
GTAACCGGGGGGACACACATACTTTTAGAAGTTAATAGAAGTCTTGAAGTCT400.12174707046111702No Hit
TCTACAGGGGTATTTACATATATTTTATATTAAGTTTCACATGAAATTATTC400.12174707046111702No Hit
TTTCTCAGGGGAGATATTACCAAGGCACTCTTGCCCACAGGGTGGCAGCTCG390.11870339369958911No Hit
GACTTTGGGAATAAAAAGCTGCCTAAGGTGAAAGTTAGAAATTGTAGACTTT390.11870339369958911No Hit
TTTTGAGGGATCCATATAGAAACTTAAGCTCTTGACCTGAATTTTTCTGGCT390.11870339369958911No Hit
CTTTGGGCAGCAAATTGGTCCCAGAATGTGCTTCGTATATTTTCCTCTTGAC390.11870339369958911No Hit
AAGCATGGGATGAGACAATTGAGTTTTACTCTTTGGAAGACTAAACCAATCA380.11565971693806118No Hit
ATGCAGGGATTCTTGACATTAAATAATTTTTTCTAGGTCTCATAGATGTTAT380.11565971693806118No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCTTATAC370.11261604017653326No Hit
TATTTTGGGACCATGCTTTGGATGCTCTAGCATTTAGATATTGAGAAAATGA370.11261604017653326No Hit
TTTTCAGGGATTTAGTAGGAATGTAAAGATACAAGTAGACAGAAAGTCTTCT370.11261604017653326No Hit
ATCATTGGGTCTTGAGCACTTAAAGTCCAGTGTTGGCTGTTAGTGTATTTGA360.10957236341500533No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCT360.10957236341500533No Hit
ATGAACGGGTATGCAAGAAGCATTGCCCTTGAGTTAAACTGTTATTGTTTCA360.10957236341500533No Hit
ATCAATGGGGGAGAGGTTGGCTTTTCCTGAATTATTCATATATAACTAAAAT360.10957236341500533No Hit
TTACGTGGGGAAGATGGAGGAGGAGATGGGATCAGAAACATAGCTGGATAAA360.10957236341500533No Hit
TTAAGCGGAATGACAGCTGTAGTAACTATGATGGGTGTAACAACATTTTTTT350.1065286866534774No Hit
TATAAGGGGCAATCAAGGTTTGAGATGCACATCATGGCTTTACATTTCTTCA350.1065286866534774No Hit
TTCTCTGGGCGATCTGAATTTGGGAAGCAGTGAGGTTGTTTGACATACAGTG340.10348500989194948No Hit
TCAATTGGGGATCTGCAAGAGGGATGTCATGGGCTATGTTACACAATTTTTG340.10348500989194948No Hit
AATACAGGGAGAGTCTGCGCTGTGACCTTCTGTTGGGCGCGTGTCTGTTGGT340.10348500989194948No Hit
GTAGATGGGATTCTGTTGCCTCAGCTGTTACTTGAAGATGGCGTAATATATA340.10348500989194948No Hit
AGGTTTGGGAGTGAGTGTTGATGAGGATGTGGAGAAAAGGGAACCCTGTGTG340.10348500989194948No Hit
TGTCAGGGGTGTGGCTCTTATCTTGTGGACCATAAATAACACGGCCCAATAA330.10044133313042154No Hit
GAACATGGGATTCCATGCTTTTCTGTGTTTATAAATCTAGGGTTACATGATG330.10044133313042154No Hit
CTCAAAAGAAGACATTTATGCAGCCAACAGACACATGAAAAAATGCTCATCA330.10044133313042154No Hit
GAATGAGGGAGATGAAATCGAGTGGCTATTGATCCGTGATGTTTCTAGATTT330.10044133313042154No Hit
GATTAAGGGGGGTTTTCTTATTTGTAAAAATAGATACTACTATCTAACTCCA330.10044133313042154No Hit
AACTGAGGGGTGGCAGATGTGGAGTGTCCCTGGACCCCGTTCAGGAAGGTTA330.10044133313042154No Hit
TTACGTGGGGAGTCTTGGGCACATTATTTAACCTCTCTGAGCTTTATTTTCC330.10044133313042154No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)