FastQCFastQC Report
Sat 31 Dec 2022
SRR5845302.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845302.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32947
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC2340.7102315840592467No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT2070.6282817858985643Illumina PCR Primer Index 5 (95% over 21bp)
TAACATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1270.3854675691261723No Hit
TCAACCGGGAGCAAAAGGAAAGAGAGGTTCTGATTCTTACCTCAGGGTTTTT1200.364221325158588No Hit
AAGTCTGGGCATCTAAATGAGTTTTACTAGCCAGGAACCTAAAAACACCCTT1200.364221325158588No Hit
AAACAGGGGTCTGGTAAGGGGACAAATAATTTGAGTATCAGAATGAAACCTT1110.33690472577169395No Hit
TAAGCAGGGCCCTTTCCGGCCGGTCCCCATGGAGGCGCTGGGGAAGCTGAAG1080.3277991926427292No Hit
GCTGTGGGTCTAACAGGTTACAGTACATTTCCTCTGTATGTAAATTAGATGG1040.3156584818041096No Hit
GAAATAGGGAGCAAGCTCCTATGTGTATATTTTCCTGAATTTCTCATCTCAA1040.3156584818041096No Hit
TACGTCGGGGAGAGCGCGCTCGGACCTCCTTCCCGGCGGCAGCTACCGAGAG1030.3126233040944547No Hit
TACAAGGGGGTCTAGACGAGGGTACCCAAACACTGGAATTGTTTGAATGTGT1020.3095881263847998No Hit
ATTAATTGGGGAAGTCTCTTTTTTGAAACACTTCAGAACTGCTGCTATAAAG1020.3095881263847998No Hit
TGTACAGGGCAGCAGCTGTTCAACAAGAGTAAATATTGTCACAACACTGAAC980.2974474155461802No Hit
GGCATTGGGACAGGCTTAAGGGCATGGACTGTATTTTATTCATATCTGAATA950.28834188241721553No Hit
TGCACCGGGAATTAAAAATTAGCTGGGCATAGTGGTGTGTGCCTGTGTTCCT940.28530670470756064No Hit
TTACCTGGGCAATCACATGTAAGGTTTTGACTAAACTAACGGATAAAGATTT930.28227152699790575No Hit
TCGTATGGGCCAGGATCCAGTGACTTGTCCTCCAACAAAACCCCTTGATCAA920.2792363492882508No Hit
TTTCGCGGGAGGACTGGGAAGCAAAGAAAATGGAGACCATGGGGGTGTGGGT910.2762011715785959No Hit
CTTATTGGGAACAACAGCTGGTGCCCTGCTTTCAGATACTGGATTGTACTTT870.2640604607399763No Hit
AACGCTGGGGAAAGGAGTTCACTCAAGTTCCCAAGGTATTCTGTCCTCCAGG860.2610252830303214No Hit
ACGGGGGCACGCATGTTCATTCATTGCTACATAATTTGGTTCTTCTGGAATA860.2610252830303214No Hit
AAAACTGGGGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACA820.24888457219170182No Hit
TTCTGAGGGGAAAAAGATGAAATTAATGAGGTCGGAGGGGCCAGATGTTTCT820.24888457219170182No Hit
AACTGGGGGAAGATGTATTTGCTAGATTGATGTGCATGTTCAGTTCTAAACC810.24584939448204693No Hit
TATGGTGGGCTGGTGTAAACTGCTCACAGAAAACCCTATGAAACCAAAGGGG800.242814216772392No Hit
TTGCTCGGGGGGGAGCTGGGGGCATGGCGTCCGGAGCGGCTCGCTGGCTAGT780.23674386135308223No Hit
CTTACCTGGGTGACAGAACAAGACCTTACCTCAAAAAAGTAAATAAAATGAA770.23370868364342734No Hit
TATAGGGGAACCACTATCACGAAAAAAACTCTACCTCTCTATACTAATCTCC760.23067350593377242No Hit
CAATCAGGGCAAGAAAATAACAGAGTAAAGACAAGAATATTTCTGCATGTTT750.22763832822411753No Hit
TTATTGGGGGCGTGTGACCCGCGGGTCGATACCTGGCAGCCCCAGTGCTGGG740.2246031505144626No Hit
CTCGTGGAGGGGATTTTGGAATTTAGTGCCCATATGCTGACAGATGGTTTAA720.21853279509515283No Hit
CGCATCGGGAGATAAGCATGTGATGCAGAAGTTGATTCAAGCAAGTGAATCC710.21549761738549794No Hit
CATCTAGGGGCAAGCCAGTCATGGAATCTTGCTGCAGGCCCTCCCTCTACTC700.21246243967584302No Hit
TAGCAGGGGTATTGCTTAAAAAATGTATAAAGCAGCTGGAAATGTTTTAAAT690.2094272619661881No Hit
CTTGCAGGGGAGCAGTCAGAGTCTTCCCTGACTCCACAATGCCCCTTTCCTT680.20639208425653321No Hit
CAGCATGGAAAATGCCAACCAAGAAAATGAAGAAAAGGAGCAAGTTGCTAAT680.20639208425653321No Hit
TCCATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA670.2033569065468783No Hit
GCTTTGGGACTTGTTCTTAATTAACAAAAGAACTAGAAAGAAGCTCATATGA670.2033569065468783No Hit
ACTTGAGGGAGGCCAAGCACAGATAGCTCAGCTGCGACCTCTGGCCTCTGGG670.2033569065468783No Hit
ACTTCAGGGTAGCCGCTAGCATCTTCCCGAGCACCGGGATCCCGGGGTAGGA650.19728655112756852No Hit
CATAGCGGCAACATCAATTAGGGAACCAAAGTTGCACTATCTGGGCCCAGAT640.19425137341791363No Hit
AGTAATGGGATCTGCAGGAGGGGAGACCAGAAGCTGTCGGAGCTGGACGACC640.19425137341791363No Hit
GCGCTGGGACTCAGTTTTAAGACAAACACTACTTCCCATGCGGGGTACTTTC640.19425137341791363No Hit
ACGAAGGGGATTGGAGCCTCCTCTACTTTTTCTTGAGGGTTGCAAGTATTGT630.1912161957082587No Hit
TTGAGGGGTATATAGAATTCCCAAAACCAGCCCATGCCAGATTTTTTCAAGT630.1912161957082587No Hit
GCCAAAGGGGTATGCCCACAATGTACATTTCTCGGCATCTGTGCCTCAGTTT620.18818101799860382No Hit
CTATAAGGGATAAATGAATAATTTAATTTAGTGCTCATTTGAAAAATATTTT610.18514584028894893No Hit
AGGTTCGGGGCCGAGCGCCACGGGCCGGCTGTGCGCGGGCCGGGGAAAGAGG600.182110662579294No Hit
GTTTAGGGGATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGAT570.1730051294503293No Hit
TCCGTAGGGATTTCTCTTTGTCATGAATTGCATACTTTGTTCCAAGTCATGT560.16996995174067442No Hit
CGTGACGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA550.16693477403101953No Hit
TTAGCAGGGGTTATGGGAAGAAGAATTTATTGAACGCTGTTTCCAAGAAATG550.16693477403101953No Hit
GCCAGGGGCACCCTGGGTTGCATACTTACTACTCAGGGGCTTTCCCCCCAGC540.1638995963213646No Hit
TACAGTGGGTCCTGATAGTGGGATGAGGAAGGTCTTCCTGTTTTCACCATCT530.16086441861170972No Hit
ATCGAGAGGGATTTTAACAATGGATCCTATAGTTAATTCTGTATAATAATTT520.1578292409020548No Hit
TCTCTTGGGGAGAGCTGTTTCCGTTCCTCTGCCCGCCATGCCGTTCCTGGAG510.1547940631923999No Hit
GCACAGGGGGGGCGAGCGACCGAGCGCCGCGACGCGGAAGTGAGGTGCGTGC500.15175888548274502No Hit
TTTCTCAGGGGAGATATTACCAAGGCACTCTTGCCCACAGGGTGGCAGCTCG490.1487237077730901No Hit
AGAAATGGGTGTGCCAACTTGCAAAAGGAATAGAAATGTCTGTGATCTAGAT490.1487237077730901No Hit
TACTTTGGGGATTGCCCGACATCGCAGAGCGAGAGCAATTCCTTCTGGGACA490.1487237077730901No Hit
TGTAATGGGATGACATGCAGCAAAAACTATTCTTCTGATAACATATTATAAG470.14265335235378032No Hit
ATTGTAGGGAGTACAGGGAATATAAAATTGGTTTCCTGTGGTCATGATCAAG470.14265335235378032No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTG470.14265335235378032No Hit
GACTTTGGGAATAAAAAGCTGCCTAAGGTGAAAGTTAGAAATTGTAGACTTT450.1365829969344705No Hit
GAACATGGGATTCCATGCTTTTCTGTGTTTATAAATCTAGGGTTACATGATG440.13354781922481562No Hit
GCTGTTGGGTAAGATGTATATTAAAGTAAAGCTACATTTTTTTACTTCATTA440.13354781922481562No Hit
ATTCATGGGAGTTTATTAAGATAGCTATGGACAGTGTAGGGGCTTAGTCTTG430.1305126415151607No Hit
TGCCAGTGGGAGAGAACAAACTTATCACTGAATGTTTGCAAGCTGGTTAAGG420.12747746380550581No Hit
AGCACTGGGCCTAAAGAACATGTGTCTTGCAAATGCCATGGAACTGAATATT420.12747746380550581No Hit
CGCGTGGGGCACCTGGGAGGAGAAGGAATTGTGGCGGGCCTAACACCTTTTC400.121407108386196No Hit
TTTTCGGGGGGAACAGATGACCTGAGATCAGAAAAGAGAGGTGCTCATTATT400.121407108386196No Hit
TTGCTGGGGATACAGGTAGAGATGTATGCAGATGTGTCCATATATGTCCATA400.121407108386196No Hit
ATGCAGGGATTCTTGACATTAAATAATTTTTTCTAGGTCTCATAGATGTTAT400.121407108386196No Hit
TTACAGGGGAATACTGATATTTTCCCCACCTCTCAATTGTTTTTAATTTTTA400.121407108386196No Hit
TTTAGCTGGGCTGGAACGTCGAAATTGTCTTCTTTGGAAAGAACCATCCCCT400.121407108386196No Hit
GCCTCCCGGGCCCAAGTGATCCTCCCGTCTCAGCCTCCTGAGTGGCTGGGAT390.11837193067654112No Hit
GGAAGTGGGAATCAAAATGGCAAATTATAAGATATTTTAAATTGGATAATAG390.11837193067654112No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGA380.11533675296688621No Hit
ATCATTGGGTCTTGAGCACTTAAAGTCCAGTGTTGGCTGTTAGTGTATTTGA370.1123015752572313No Hit
AAACAGGGGAACCTTCCTTAAGTACAATTTAACTTCAAGAAAACAAAATTTG370.1123015752572313No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCT370.1123015752572313No Hit
TATAAGGGGCAATCAAGGTTTGAGATGCACATCATGGCTTTACATTTCTTCA370.1123015752572313No Hit
TATTTTGGGACCATGCTTTGGATGCTCTAGCATTTAGATATTGAGAAAATGA370.1123015752572313No Hit
GCTCTTGGGAAGGATAGAGTGATGACATTTATTTTCATTCACTTTGAATCTG360.10926639754757642No Hit
AATGTTGGGACAGAAACCTAGCCATTCAAAATCTGTTTAAAGGACAAAGTTT360.10926639754757642No Hit
TCAATTGGGGATCTGCAAGAGGGATGTCATGGGCTATGTTACACAATTTTTG350.10623121983792151No Hit
AATACAGGGAGAGTCTGCGCTGTGACCTTCTGTTGGGCGCGTGTCTGTTGGT350.10623121983792151No Hit
GAAATTGGGTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTC350.10623121983792151No Hit
CTTTGGGCAGCAAATTGGTCCCAGAATGTGCTTCGTATATTTTCCTCTTGAC350.10623121983792151No Hit
TGTCAGGGGTGTGGCTCTTATCTTGTGGACCATAAATAACACGGCCCAATAA340.10319604212826661No Hit
CAACAGGGATAGAATGTAAGCAGAAATAATGTCATAAGCAATTTTATATGAA340.10319604212826661No Hit
GCGCAGGGATTGAAATCTGCAGTGAGATATAACTTTCTAGGAACAACAAAAT340.10319604212826661No Hit
TCCAAAGGGACTTTCATTATCTGTGCCTTATAGAAAGATCTGTAACTAAATC340.10319604212826661No Hit
AACTGAGGGGTGGCAGATGTGGAGTGTCCCTGGACCCCGTTCAGGAAGGTTA340.10319604212826661No Hit
AGCTAGGGGGAATAATATGGTAAGGTACTCAATTTATTTAAAAGATCTAGAC330.10016086441861172No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCTTATAC330.10016086441861172No Hit
TTAGGTGGGGGAAAGTGAGGCTTACCAATCACAACTTCTAGTAAATTAAAAG330.10016086441861172No Hit
TTGGCTGGGATTTTAATAAATTTAAAATTAACTATGAAATGTCATATGAATG330.10016086441861172No Hit
CACTTAGGGGTACTTTTAAAAAGATTAGCTTGGCAACAAGTCTAGCCTGAAA330.10016086441861172No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)