FastQCFastQC Report
Sat 31 Dec 2022
SRR5845301.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845301.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32862
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT2280.693810480189885Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC2010.6116487127989776No Hit
AAACAGGGGTCTGGTAAGGGGACAAATAATTTGAGTATCAGAATGAAACCTT1100.33473312640740066No Hit
TAACATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1090.3316900979855152No Hit
TGCACCGGGAATTAAAAATTAGCTGGGCATAGTGGTGTGTGCCTGTGTTCCT1060.32256101271985876No Hit
TACGTCGGGGAGAGCGCGCTCGGACCTCCTTCCCGGCGGCAGCTACCGAGAG1040.31647495587608787No Hit
GGCATTGGGACAGGCTTAAGGGCATGGACTGTATTTTATTCATATCTGAATA1020.310388899032317No Hit
TAAGCAGGGCCCTTTCCGGCCGGTCCCCATGGAGGCGCTGGGGAAGCTGAAG1020.310388899032317No Hit
TTGAGGGGTATATAGAATTCCCAAAACCAGCCCATGCCAGATTTTTTCAAGT1000.30430284218854603No Hit
GCTGTGGGTCTAACAGGTTACAGTACATTTCCTCTGTATGTAAATTAGATGG1000.30430284218854603No Hit
TCAACCGGGAGCAAAAGGAAAGAGAGGTTCTGATTCTTACCTCAGGGTTTTT990.3012598137666606No Hit
ATTAATTGGGGAAGTCTCTTTTTTGAAACACTTCAGAACTGCTGCTATAAAG970.29517375692288966No Hit
AAGTCTGGGCATCTAAATGAGTTTTACTAGCCAGGAACCTAAAAACACCCTT970.29517375692288966No Hit
CTTATTGGGAACAACAGCTGGTGCCCTGCTTTCAGATACTGGATTGTACTTT940.2860446716572333No Hit
AACTGGGGGAAGATGTATTTGCTAGATTGATGTGCATGTTCAGTTCTAAACC900.2738725579696914No Hit
ACGGGGGCACGCATGTTCATTCATTGCTACATAATTTGGTTCTTCTGGAATA890.270829529547806No Hit
TATAGGGGAACCACTATCACGAAAAAAACTCTACCTCTCTATACTAATCTCC890.270829529547806No Hit
TCGTATGGGCCAGGATCCAGTGACTTGTCCTCCAACAAAACCCCTTGATCAA880.2677865011259205No Hit
TAGCAGGGGTATTGCTTAAAAAATGTATAAAGCAGCTGGAAATGTTTTAAAT880.2677865011259205No Hit
GAAATAGGGAGCAAGCTCCTATGTGTATATTTTCCTGAATTTCTCATCTCAA860.2617004442821496No Hit
ACGAAGGGGATTGGAGCCTCCTCTACTTTTTCTTGAGGGTTGCAAGTATTGT820.24952833059460774No Hit
CTTGCAGGGGAGCAGTCAGAGTCTTCCCTGACTCCACAATGCCCCTTTCCTT810.24648530217272227No Hit
TTTCGCGGGAGGACTGGGAAGCAAAGAAAATGGAGACCATGGGGGTGTGGGT800.24344227375083685No Hit
TTATTGGGGGCGTGTGACCCGCGGGTCGATACCTGGCAGCCCCAGTGCTGGG790.24039924532895138No Hit
TCCATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA770.23431318848518046No Hit
TGTACAGGGCAGCAGCTGTTCAACAAGAGTAAATATTGTCACAACACTGAAC770.23431318848518046No Hit
TACAAGGGGGTCTAGACGAGGGTACCCAAACACTGGAATTGTTTGAATGTGT770.23431318848518046No Hit
CTATAAGGGATAAATGAATAATTTAATTTAGTGCTCATTTGAAAAATATTTT720.21909804637575314No Hit
TACAGTGGGTCCTGATAGTGGGATGAGGAAGGTCTTCCTGTTTTCACCATCT710.21605501795386767No Hit
TTACCTGGGCAATCACATGTAAGGTTTTGACTAAACTAACGGATAAAGATTT700.21301198953198225No Hit
CTTACCTGGGTGACAGAACAAGACCTTACCTCAAAAAAGTAAATAAAATGAA700.21301198953198225No Hit
GCGCTGGGACTCAGTTTTAAGACAAACACTACTTCCCATGCGGGGTACTTTC700.21301198953198225No Hit
CTCGTGGAGGGGATTTTGGAATTTAGTGCCCATATGCTGACAGATGGTTTAA680.2069259326882113No Hit
AACGCTGGGGAAAGGAGTTCACTCAAGTTCCCAAGGTATTCTGTCCTCCAGG670.20388290426632585No Hit
CATCTAGGGGCAAGCCAGTCATGGAATCTTGCTGCAGGCCCTCCCTCTACTC670.20388290426632585No Hit
CGCATCGGGAGATAAGCATGTGATGCAGAAGTTGATTCAAGCAAGTGAATCC650.1977968474225549No Hit
TCCGTAGGGATTTCTCTTTGTCATGAATTGCATACTTTGTTCCAAGTCATGT650.1977968474225549No Hit
CATAGCGGCAACATCAATTAGGGAACCAAAGTTGCACTATCTGGGCCCAGAT640.19475381900066946No Hit
ACTTCAGGGTAGCCGCTAGCATCTTCCCGAGCACCGGGATCCCGGGGTAGGA630.191710790578784No Hit
CAATCAGGGCAAGAAAATAACAGAGTAAAGACAAGAATATTTCTGCATGTTT630.191710790578784No Hit
CAGCATGGAAAATGCCAACCAAGAAAATGAAGAAAAGGAGCAAGTTGCTAAT630.191710790578784No Hit
ATCGAGAGGGATTTTAACAATGGATCCTATAGTTAATTCTGTATAATAATTT610.1856247337350131No Hit
GCTTTGGGACTTGTTCTTAATTAACAAAAGAACTAGAAAGAAGCTCATATGA610.1856247337350131No Hit
ACTTGAGGGAGGCCAAGCACAGATAGCTCAGCTGCGACCTCTGGCCTCTGGG610.1856247337350131No Hit
AAAACTGGGGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACA600.18258170531312762No Hit
ATTGTAGGGAGTACAGGGAATATAAAATTGGTTTCCTGTGGTCATGATCAAG580.1764956484693567No Hit
CGTGACGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA570.17345262004747125No Hit
TTGCTCGGGGGGGAGCTGGGGGCATGGCGTCCGGAGCGGCTCGCTGGCTAGT570.17345262004747125No Hit
GCCAAAGGGGTATGCCCACAATGTACATTTCTCGGCATCTGTGCCTCAGTTT560.17040959162558578No Hit
AGTAATGGGATCTGCAGGAGGGGAGACCAGAAGCTGTCGGAGCTGGACGACC550.16736656320370033No Hit
GCCAGGGGCACCCTGGGTTGCATACTTACTACTCAGGGGCTTTCCCCCCAGC530.16128050635992938No Hit
TATGGTGGGCTGGTGTAAACTGCTCACAGAAAACCCTATGAAACCAAAGGGG520.15823747793804394No Hit
TTGCTGGGGATACAGGTAGAGATGTATGCAGATGTGTCCATATATGTCCATA510.1551944495161585No Hit
TTTAGCTGGGCTGGAACGTCGAAATTGTCTTCTTTGGAAAGAACCATCCCCT510.1551944495161585No Hit
TACTTTGGGGATTGCCCGACATCGCAGAGCGAGAGCAATTCCTTCTGGGACA510.1551944495161585No Hit
CGCGTGGGGCACCTGGGAGGAGAAGGAATTGTGGCGGGCCTAACACCTTTTC490.14910839267238757No Hit
AGAAATGGGTGTGCCAACTTGCAAAAGGAATAGAAATGTCTGTGATCTAGAT490.14910839267238757No Hit
GTTTAGGGGATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGAT490.14910839267238757No Hit
TTCTGAGGGGAAAAAGATGAAATTAATGAGGTCGGAGGGGCCAGATGTTTCT490.14910839267238757No Hit
GAACATGGGATTCCATGCTTTTCTGTGTTTATAAATCTAGGGTTACATGATG480.14606536425050212No Hit
AGCACTGGGCCTAAAGAACATGTGTCTTGCAAATGCCATGGAACTGAATATT470.14302233582861665No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTG470.14302233582861665No Hit
GTAACCGGGGGGACACACATACTTTTAGAAGTTAATAGAAGTCTTGAAGTCT460.13997930740673117No Hit
ATGCAGGGATTCTTGACATTAAATAATTTTTTCTAGGTCTCATAGATGTTAT450.1369362789848457No Hit
TTTCTCAGGGGAGATATTACCAAGGCACTCTTGCCCACAGGGTGGCAGCTCG440.13389325056296025No Hit
CAATGAGGGAAGCTGTAAGAATGAAGCAATGAATCAAGACTTCTACCCAAAG440.13389325056296025No Hit
TATAAGGGGCAATCAAGGTTTGAGATGCACATCATGGCTTTACATTTCTTCA430.1308502221410748No Hit
GCCTCCCGGGCCCAAGTGATCCTCCCGTCTCAGCCTCCTGAGTGGCTGGGAT430.1308502221410748No Hit
GCGCAGGGATTGAAATCTGCAGTGAGATATAACTTTCTAGGAACAACAAAAT420.12780719371918933No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCT420.12780719371918933No Hit
CAAAATAGCTGTCTGTGTTGCAATCATTGCAAAATCTAAATTCTTTTGTTAC420.12780719371918933No Hit
AGGTTCGGGGCCGAGCGCCACGGGCCGGCTGTGCGCGGGCCGGGGAAAGAGG420.12780719371918933No Hit
TTAAGCGGAATGACAGCTGTAGTAACTATGATGGGTGTAACAACATTTTTTT410.12476416529730387No Hit
ATCAATGGGGGAGAGGTTGGCTTTTCCTGAATTATTCATATATAACTAAAAT400.12172113687541843No Hit
AAGCATGGGATGAGACAATTGAGTTTTACTCTTTGGAAGACTAAACCAATCA390.11867810845353295No Hit
ATCATTGGGTCTTGAGCACTTAAAGTCCAGTGTTGGCTGTTAGTGTATTTGA380.11563508003164749No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCTTATAC380.11563508003164749No Hit
TTAGCAGGGGTTATGGGAAGAAGAATTTATTGAACGCTGTTTCCAAGAAATG370.11259205160976204No Hit
GTAGATGGGATTCTGTTGCCTCAGCTGTTACTTGAAGATGGCGTAATATATA370.11259205160976204No Hit
CAGGCGGGGTGATTTAAGAAGTGGCTGGATTGGAACTTTTAATATGCTAATG360.10954902318787657No Hit
TTGTAAGGGAACTAAAACATTAACCAAAAGAAAGCATTTTAAACCAAAAGTG360.10954902318787657No Hit
AATACAGGGATTCCTAACTTTATAAAGTTAGAGCAAAAAGATTTGCGGATAG350.10650599476599112No Hit
TCAATTGGGGATCTGCAAGAGGGATGTCATGGGCTATGTTACACAATTTTTG350.10650599476599112No Hit
TTGTCGGGACTGCGGACCTCCAAAATTTTACAAAGGACTGCACAGTGAGTAT350.10650599476599112No Hit
AACTGAGGGGTGGCAGATGTGGAGTGTCCCTGGACCCCGTTCAGGAAGGTTA350.10650599476599112No Hit
AAACAGGGGAACCTTCCTTAAGTACAATTTAACTTCAAGAAAACAAAATTTG340.10346296634410565No Hit
TGAACTGGGATTGCCACAGTGGTTTTATTTTTATTGTGTGATGTAACCATAT340.10346296634410565No Hit
AGTAGCGGGATGCCTGGCTAATTTTCTTTCTATTTTTTGTAGAGACAGGGTC340.10346296634410565No Hit
AGTTTCGGGGATTATATTTTCATTTGCTTCTTCCAGGAATTTTAGGTATATA340.10346296634410565No Hit
TCTCTTGGGGAGAGCTGTTTCCGTTCCTCTGCCCGCCATGCCGTTCCTGGAG340.10346296634410565No Hit
AAATGCGGGTGCGCGAGGGATGAATAATATGAAGGAGAGGTAGATTTCTGAG330.10041993792222019No Hit
TGCTCACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC330.10041993792222019No Hit
TCTAGTGGGATGGTGGTGTGCATTTGGAACTGGGCATGCAAACTTGAATTTG330.10041993792222019No Hit
GGAAGTGGGAATCAAAATGGCAAATTATAAGATATTTTAAATTGGATAATAG330.10041993792222019No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)