FastQCFastQC Report
Sat 31 Dec 2022
SRR5845300.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845300.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27852
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT2730.9801809564842741Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC2410.8652879505960075No Hit
CAAAGAGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATT1840.6606347838575327No Hit
GCTTTGGGGCATGTTTGGTCCCGACATCTGTGGCCCTGGCACCAAGAAGGTT1830.6570443774235243No Hit
CTAGCGTGGGGCTTTATAAAAAGTAATATTAAAATTTTTAAAAGTGTATAAA1770.6355019388194744No Hit
CAACGGGGGATGCAGAAAGCTTTCATTTGCAGAGAGCTTGCTATTTGGAGAG1750.6283211259514577No Hit
TACGCAGGGGACCTGGATGCTGAAGCTGCCATGGACATCTCAGAGGGCCGCT1650.5924170616113744No Hit
TAATGTGGGCAGAAAGGCCAGCGGGGAGAGGCTGGGGCGAGGGGAGGAGGGG1640.5888266551773661No Hit
TTCAACGGGTGAGAAGATTCTGCGTGGTCTTGCAGTTGGCATAGCTTTAGTA1600.5744650294413327No Hit
TGCATGGGGCGATCTTTAGTTGGGTGTTGACTTCATCTTCCTTAACTGTCAA1600.5744650294413327No Hit
ATACATGGGCAGTGGTGACAGGTCTAGCTTATTTCAAGAGCTGCACAAAAGC1540.5529225908372828No Hit
GATTTTGGGTAAGCTGATGAATAGTCCAAAAGAAGCCTCAGAATTTACTTAT1510.5421513715352577No Hit
AATCCGGGAAGATTGATCTATATATTTGATATAATCACAATCAAAATCCCAG1480.5313801522332329No Hit
ATTCGGGTAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGAT1450.5206089329312078No Hit
CTTGTGGGAGAATTATTTCCTTTGTGTTCGTGAGTTGTTTTAATAGGAATTT1430.5134281200631912No Hit
ATGACAGGGTTGATCAGCAAATGGAACATTCTCAGCAGAGGGAAAATGAACA1380.4954760878931495No Hit
CTTCTAGGGGGTGGCACCGCTGGAGGGGGCGGCCTGGGGCTGGGGCTGCCCG1360.4882952750251328No Hit
AAGTACGGGTTATCTATACAGTTTTTTCAGTGCTAAAATATTCTGGATAAAT1340.4811144621571162No Hit
TCAAACGGGCAACTCAAAGAGAGCACACACCATGTCTGTTTTGTTCACTAAT1300.4667528364210829No Hit
CTATTGGGGGTGGAAATTTGCAGAGGGGCAGGTGTGTGGTAAACGGGTAATG1250.4488008042510412No Hit
AACTCAGGGAACTGACATAGATTGTCAATTTTTAAAAAATTTTCAGATTGAT1210.4344391785150079No Hit
CAATCGGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1210.4344391785150079No Hit
AGACGGGGGACCTAATATCAGAGCTGCAAATACATCAAGCAAAAATTAATGA1190.4272583656469912No Hit
CGCAAGGGGTGACTAGTTGAGTGATTTGCCCCAAGTTATACAACTTTAAAAT1180.4236679592129829No Hit
GTACTTGGGAAACTCAACCTTGTACTCATATAATGAAGAGAATAACAAGAGT1170.4200775527789745No Hit
ATTACCGGGGAGTGGTGCTGGGAGTGTCGTGGACGCCGTGCCGTTACTCGTA1160.4164871463449663No Hit
AGTGGCGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1160.4164871463449663No Hit
GATTGGGGCTGTGGGCCATTGTGTTTATCTTGGGTAGACTTGTGACTTTAAT1120.4021255206089329No Hit
TTACGAGGGGAATTGGTTCTTTCTCTGTAAAACAAATGTAACTATATTTATT1100.3949447077409162No Hit
AAAGTTGGGGAGAGCCGTCGTGGCTCGTTCCATTCTCGGCGGTGGTACCTGC1090.3913543013069079No Hit
ACGCATAGGGATAATAGGGATTTGATAAGTGTTCATTACAGTTTAAAAAATG1090.3913543013069079No Hit
CTAAAAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1070.38417348843889126No Hit
TCATAAGGGAATATATAAATATGGTGTCTGTGTACATATAGTGAAGATATGC1070.38417348843889126No Hit
GGACACGGGGCCAGAAAAACCTGTTGATCAAACAAAGCTGTTTATTAGCTTA1060.38058308200488294No Hit
GTTAATGGGATTCTTCTACTGATGAACACTTAGGTTGTTACCATATCTGGAC1060.38058308200488294No Hit
GTTTACGGGATACTAAACACTGTTTATGTAATAGCATAGTGCTGGTTTGAAT1060.38058308200488294No Hit
TTATATGGGGGGCTCTCGATGGTGGCGTGACGGGGGCGGGGGTGGCGGCGCG1060.38058308200488294No Hit
GAATACGGGATTCAGTATTAACGTAGCAACATCATCATACTTTTAATTAGTA1040.3734022691368663No Hit
GCTTATTGGGGAAGGCTGGCTTCCATGTGCAGCACTCTTCCCAATTGCCAGG1040.3734022691368663No Hit
GGTAATGGGAACTCCTAATATTCTGGCTTTGTTCTACCTGTAAGCTTCTCTG1030.36981186270285793No Hit
GCGACTGGGATTGAGTAGAGGCCTAAAGAGGACTGTGCATTTTTGCTATTTA1010.3626310498348413No Hit
TGCCGCGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1000.35904064340083297No Hit
GTAGTAGGGAGGGAGCCGGAGGGGACGCGCCGGAGGAAAGATGGAAGACTAC990.35545023696682465No Hit
ACAAAAGGGTCGCGGGGGCAATTTAGGAATGCTCAATGTGCTGTTGATTCTT980.35185983053281633No Hit
CCGATAGGGCATGCGGCCTAGCTCGGTTCCGCCTCCTCCTCGCGCGGCCCCA980.35185983053281633No Hit
CATCGGGGGAGTAACTTTCATGACATAGGATTCTGCCGCCAAATTTATATCA980.35185983053281633No Hit
ATAGTGGGGACATCAGCTCTCAGTAACCCTAGAAGACTTATATAATGGTGCA960.34467901766479964No Hit
ACTTTGGGGGAATGTTTTTCTTATAAAAAATTATACAAATGCTGAAGTGAGA940.337498204796783No Hit
TGCATCGGGAGGATGAGCTATGGAAGGAGTGAACACTGGAGCAGGAGTATAG930.3339077983627747No Hit
GAATAGGGATAACAGTTTCAGTGAATAGAGAAAAAATTCTGAACTGATCATA930.3339077983627747No Hit
GCTCATGGGATTGGGTTTCTTAGCTGTGCATGCAATTTGAATAATTACTACA930.3339077983627747No Hit
ACACGACGGGCTTTCTAGATTTACTGTCTAAATTTAACCTTTCCAAAGCATT920.33031739192876636No Hit
TAGCTCAGGGTACTGGCTGTGGCTGGAATCTGCCTTTTATCACAGCTGTCAC910.326726985494758No Hit
AGTAGCGGGGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAG850.305184546890708No Hit
ATTTAAGGGAATGTGTTTGGAGCACTAAATACGACTTGAGTGCTTGCTCATT840.3015941404566997No Hit
GACGTGGGGATTTTTTTAAAGTTACATTAAGATTTTTTCTCTTTTGCAGCTA840.3015941404566997No Hit
CTGAGTGGGGGATGATTGTGGATCCCCTGAGATCTGGCTTCCCAAGAGCACT810.29082292115467473No Hit
GTTCGCGGGGAGTCTTACCTCGTTGTGGCGTCAGAACCGCCGTCGCTCGCTC810.29082292115467473No Hit
AATAAGGGGAGATGTTGTTGATAGTCATCTTTAAACTGAGTTAAAAGATGCT770.2764612954186414No Hit
TTGCATGGGTATGTGATAATATGCTTAAAATGAAAGCAAGTTCCATATTTGT760.2728708889846331No Hit
ATTGTTGGGAAATCATATTTCAACAAAGCTGTTTTAAGAAAAATCATATTTC760.2728708889846331No Hit
AAGCGTGGGCGATGACATGAAAGTGAAGTAAATTTATTCTATGTAAATTCAC760.2728708889846331No Hit
CTGAAAGGGACACATATGAAAAGTACTGGCCATTTTACCAGAAGCAAGGAGG760.2728708889846331No Hit
ACACTTGGGCAGCGGCCCGGGGAACCCGCTCCCAGACAGACTCGGAGAGATG750.2692804825506247No Hit
ATTCTAGGGGCATATATATATTTATACCACCATGGACTTACTTTCACACTGG750.2692804825506247No Hit
CAGTCTGGGGGGGAACATTCTCAAGAGGTGAAATACAGAAAGCCTTTTTTTC750.2692804825506247No Hit
TACAGTAGGATCCTACTGTCAGCATTGGACAGTTCATCTAGACATAAAATCA740.26569007611661644No Hit
AAGTTGGGGAGGCCCCAACGTTGTAGGCCCCTACGGGCTACTACAACCCTTC740.26569007611661644No Hit
CTTGTCAAGGGATAAATTAAAATCAGCAGATTGAGTTTTCAACATTGCAAAA720.25850926324859974No Hit
TAGCGGGGACAAACAAATGTGAAGATATAATGTGTATTTTATTTGAATGTCT710.2549188568145914No Hit
CAGCATGGGATATGAAGCCTGCATATACTGTGAGCTGTGATTGGGGAACACC710.2549188568145914No Hit
ACCTCGGGGTATTAATTTACTATGAAATGGACATCCTTTTAACTAAAATGGA700.2513284503805831No Hit
TTATAGGGATTCACTCCCAGTTGGATCATTTACTGGATCCTTCTTCTTTCAC700.2513284503805831No Hit
TGGCTGGGGAACAATGAAGAATGTGGTTCCCAATATCTTCAATTTTTCTCAT690.24773804394657475No Hit
GAGTCAGGGGTGCTCATTCTCCCTAAATAGTAAATCCCACTGTATACAAAAC690.24773804394657475No Hit
TCATTTGGGTATGACTGATAGCAGAATAATAATGCATCTTACTTCTATATAG670.2405572310785581No Hit
ATGAGGGGTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGC670.2405572310785581No Hit
GCTACCCGGGGCACTATTCATCATTGTATCTTCACCATCTAGCACGGTGCTA660.23696682464454977No Hit
TGCGCTGGGAGCCGTGGGGCCAATTTTTTAACTCAGATCTTGCTGAGACCAG660.23696682464454977No Hit
ATTCTGGGGGGAAGGCACATTGTATCAATTCTACCTTGTGCTATACGTAGGA650.23337641821054145No Hit
CATTTGGAAGATAGAAAATGTATTAGAGTTCTTCAGAGAAGCAGAACCAATA640.2297860117765331No Hit
CTACTCAGGGGATCTTGCTATGGGGACGGTGATATTGGAAATTATTATTATG640.2297860117765331No Hit
ATTTGGGGGCATGGATAATTATTTTAACAATGCTAATAGTTGAGTTTTGCAG640.2297860117765331No Hit
TAACGTGGGGGGGGCTCTCTTTGCTTCGAGAAGTAAACCTAATACCATTTTT640.2297860117765331No Hit
TATGAGGGGCTGCGATGGCTGAAATGACAGAAATAAAATTTGGAATATGGAT630.22619560534252475No Hit
CTTTTAGGGATGCTTTTTCTGAATGGACAGGAGAAACATAAGCTACGGAGTA620.22260519890851646No Hit
TGCAGTGGGATTGCTGCATTCATGCAAGACTGAAATTTATTATTAGACAAAT590.21183397960649145No Hit
TGTGCTTGGGTATAACAGCCTTTTCCTTCTGGTTCTAACTGCTGTTAAAATT580.20824357317248315No Hit
GAACAGGGGAACTTGGTAACTTCTGGGTTTAGTAGAGCCTCAGTGTCGCTTT580.20824357317248315No Hit
AAATACGGGATGGATTAATGTAACAGAATACCTAGCTATTACTTTAGCAGAA580.20824357317248315No Hit
TGCAATGGGAAACAAAATATATGACAATAGCACAAATACCAAGAGGATAGAC570.2046531667384748No Hit
GCACATGGGACGGGAACACAGCGCTGACCTACGCCCGGCAGGCCTCCAGCCA560.20106276030446646No Hit
TACACAGGGAATAATGCTCACTTCTGATTTGATGTTGATATACTTTTATGGT540.1938819474364498No Hit
CTTAAAGGGTGGCAGATAACTTATATATATGAAAAAGTATAGTAAGAAATTT540.1938819474364498No Hit
TCCTCAGGGGGAAAAGAAATTAGAATGTCTAAATAACATGTATCATCCAGGA540.1938819474364498No Hit
CTAGCGGGGAGTGGGAGAGACCCTAATAACTATTTTGGTGAAGGATGAATCT540.1938819474364498No Hit
ATGTATGGGATCTTAAAATGTTCCTTCTCACTTATTCTACTGGAATTGACCA520.18670113456843315No Hit
GCTGCTGGGATCTGTAAAAGGTTTCTCCCTACTGTCAAAAAAATATGCATGT520.18670113456843315No Hit
GAACCAGGGATAATCCATATTTGCTGTTCAAGTTAATCTAGAAATTTATTCA520.18670113456843315No Hit
AGCACAGGGATGAAAGACATAACTACTATCCCTCTTTATCAAGGTCTCATGT500.17952032170041649No Hit
TTCACCGGCATACCTCTGCTTTATCTTTTGCATTTGATCATTCATATGAAAA500.17952032170041649No Hit
TAGCGTGGGAGTACCTGAATTCTTTGGCTGTGAAAGCATATATTCTAGTACT500.17952032170041649No Hit
ATCGCCTAGGGACAAAAACACGTGGCCAGGTCAGGCCCTGTGTTTCCTGGCT500.17952032170041649No Hit
TGTTCTGGGAGAACTCTCAATCCAGGGCTGTTTCCTCTATTTGTAGCTTAAT490.17592991526640817No Hit
GTGGGTGGGATGTCACTGTCATTCATTACATTTTAATTTTTGAAACTGATGT490.17592991526640817No Hit
GAATTAGGGATTGGAAGACTAAAGCAGCAAATATGTCTTCATTGACAAAAAT490.17592991526640817No Hit
TAATGGGATACTAGGAACATTTGAATTATTTTTTTCTAGTTATTTTTAAATA470.1687491023983915No Hit
GGCTTTGGGATGTTGACTGAATGACTATGAGGAAAAACAAGTATTATTAACA470.1687491023983915No Hit
TATAAGGGGGATGAGTTTCTTAGAAAAATATAATTATAAAAATTTACTTAAA460.16515869596438318No Hit
TGTATGGGGATATGTTTGAGGATTTTTCTTTTCCTTTTTAAATTTTTTTATT460.16515869596438318No Hit
CGCTCAGGGTACGCGGGGGACATTTGAGTGCTTTCGTAAGCCCTGTACATGT440.1579778830963665No Hit
TCTAACGGGATTTATTTATTTATATAAACAAAAATATAGAGATCTTACCTTG440.1579778830963665No Hit
ATCTAAGGGCGATCTGGTTTCGGGCTCTCAGAGGAGTACTTAGGGAAGTCCA440.1579778830963665No Hit
TTTTCAGGGACGGTTGTATTTTCTGATCATTGGTTTCTTAATATGGTTGTAC440.1579778830963665No Hit
TAGCTTAGGGGGGAATTGAGAATTTATTTGAAATAGAATAGGTAACACCTCA430.1543874766623582No Hit
GACGAGGGGCGTCTCCTGTGGCTGGGACAAGCTGGTCAAGGTATGGAACCTG420.15079707022834984No Hit
CTACGGGGTGTGCATCTGAGAGATGTCAAAGCTTTTCAGAGTCAAAATGAGA410.14720666379434152No Hit
CGCTCTGGGCAAGGAGATCATTCCGTGTATATATCTCCATCAATCATACTTC410.14720666379434152No Hit
TGGTAGGGGTTTTTAGGATAGTGTAAATGGGTATTTAGATGATATCAAATTC410.14720666379434152No Hit
TTTCTCGGGATGGAGATGTGTGTCCGAGGCTACCTGGCTGTGCGGCCCTACA410.14720666379434152No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCTTATAC410.14720666379434152No Hit
ATAGCTGGGATTGTACAGATTTGTCTGTATTTCTCACCATATCTAATGATAC410.14720666379434152No Hit
GTATCAGGAAATGTAAGAATACTTTAAATCTCTAAGCTTCTGAAGTGTTAGA400.14361625736033318No Hit
TACCAGGGAGGTCTTTTAAATGAGTCATTTTCAGCTTTTTTAAACACTTAGT400.14361625736033318No Hit
CCATGAGGGACTGAAATATTTATAGATGAAATGCCTGGAATTTTCTTTAAAA390.14002585092632486No Hit
AATACAGGGTTTGCCATCTCTGCTCCAACCGGGATGGGAGCCGGGCAAACTG390.14002585092632486No Hit
GTACGTAGGGAGGGGTTATGTTAGTATTCAAAGGGATGCTGTAAAGATAAGA390.14002585092632486No Hit
CTGCAAGGGATAGTCTTTCTTAGAACCATTTTTATGGTTTTCATAAAATTGC390.14002585092632486No Hit
TGACCGAGGGAATGCCAAAATTCCATTGAAACTTTCAAGTTGGAGCAATTGT380.13643544449231654No Hit
TTGACGGGGAAGTCATGAAAGCTGCTAGATTTTTTTACAGATCTGGAAACTT380.13643544449231654No Hit
GTCTTTGGGTGCAAACAGTACCTAACAAACCCACAGGTCCTAAACTACCAAA380.13643544449231654No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTG380.13643544449231654No Hit
TGCCGTGGGGTTCCTTCCCATGTGCTTCTCCCCAAATTTTCATATAGATATA370.13284503805830822No Hit
TGCGATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG370.13284503805830822No Hit
TCGTCTGGGATCCCACTTGGTTTTTGACTGAAGGGGAAGTGTAGAAATATAT370.13284503805830822No Hit
CTTATAGGGACTTCACAGACTCAATGAGGCAGAAATTATTGTAGTTTTCTCC370.13284503805830822No Hit
ATGCTGGGGGCCCCACCCTTGACACGTGGGAATTATTACAATGCAAGGTGAG370.13284503805830822No Hit
TTTTATGGGCTCCAGGCCAGCATTGTTGGTGGTGATTGCTCTGGACCTCACT360.12925463162429987No Hit
TCGCGTGGGAGCTGACAGCTTTTCAGGGGTGGTCTGAAAAGCCAGTTTGACA360.12925463162429987No Hit
AGGTTCGGGTCCACTTGTTTATTGTGGCACTTGCTGGAGCTGGGGCAGCAGT360.12925463162429987No Hit
TGTTCAGGGTGGCTTTTGGAGAGACTTTGTCTACCACAAGAATAGAGTAAAA350.12566422519029155No Hit
TGCGCGGGGAGACCATGGTCCCTAAACCTGCAGTTTCTTCCTGCTGGTGCAG350.12566422519029155No Hit
TACTAAGGGTATCACAGCATGGGGGCTGGCTCCCCTCGGAGTGGATGGCCTG350.12566422519029155No Hit
AGCGCAGGGAGATGGGTGCTGGTCCTCAGGCGTTGGGGACACTGCTGGGTCG340.1220738187562832No Hit
GTAGTTGGGATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT340.1220738187562832No Hit
TACAGCGGGGTCCAGGTAAGAGGGCCTCACACAGGAGGGCTTGCTCAGAACC330.11848341232227488No Hit
GCCTATGGGCAGCACTCAGCTGCATGCGGGACAGGTGAGCCTGCAGGAGCAG330.11848341232227488No Hit
CTGTTCGGGAGTTGGAGATTTTAACACTAATCTTTCAGTCATTGAGAGAATT320.11489300588826655No Hit
TCATGGGGGAAGCCTGTGGTTGAGGTTTCCTTTAGTCTTTGGGAAAAGTATG320.11489300588826655No Hit
ATCTATGGGATAAAATGATGGTTCTTTACTAAGCTGATATTTTTTATTGTAA320.11489300588826655No Hit
CATGCAGGGTTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTTAAGAG320.11489300588826655No Hit
TGTCTGGGGGAGTACCCTGCACAGCTTTGCAGTTAAGGACACCTTCTGGGAG320.11489300588826655No Hit
CCTTTAGGGAAGTAAAGTTTCCTGCGCACCTTATACCAGAATTCAGTATAAT310.11130259945425823No Hit
CGCACAGGGACACGACGGCTCTTCCGATCTCAAGAGGGGTGCTCTTTTATTT310.11130259945425823No Hit
AGCTAAGGGAAGCCAATACCTTTCTCTCTCTTTCTCTCTTTTCTCTCTCTTA310.11130259945425823No Hit
TATGCAGGGCCTTGACATTTATTTGTTAATCATATGATCATTTTTTTTTCAC310.11130259945425823No Hit
ATGCATGGGCTGTTGTGGCAATACTATGTGGTTTCTGTTACAACAATTATTA300.1077121930202499No Hit
CAATTAGGGTATATTAATTGCCAATATAAGTAAATATAGATTATATATGTAT300.1077121930202499No Hit
CTATCAGATTCTATAATAGTATAGTTATTAAGGCAATTTTATGTTAGAGACT290.10412178658624158No Hit
CGATAAGGGGCTGCGGAACTTTCCTTTTTTAATAAGACCATATTATGACTCT290.10412178658624158No Hit
GTTAAAGGGAAGCAGTACTTTTGGAACTGAGTAATAACATTTATTTGCCACT280.10053138015223323No Hit
CTCTTAGGGAAGGGTCTATAATTGCGTATAAACAGAAAAGTAAAGACTTACA280.10053138015223323No Hit
CTAATAGGGGTTTTAGATAGAAAGTGAGCCAGTCAGAGAGAGAGACTGACTT280.10053138015223323No Hit
TACTAAGGGCGATCTGCTGGATGGGGCAAGCAGATCTGTTGTATGTAATAAG280.10053138015223323No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)