FastQCFastQC Report
Sat 31 Dec 2022
SRR5845299.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845299.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27811
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT2690.9672431771601165Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC2420.8701592894897702No Hit
CAAAGAGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATT2080.747905505016001No Hit
GCTTTGGGGCATGTTTGGTCCCGACATCTGTGGCCCTGGCACCAAGAAGGTT1820.6544173168890007No Hit
CAACGGGGGATGCAGAAAGCTTTCATTTGCAGAGAGCTTGCTATTTGGAGAG1810.6508216173456546No Hit
CTAGCGTGGGGCTTTATAAAAAGTAATATTAAAATTTTTAAAAGTGTATAAA1660.5968861241954622No Hit
TTCAACGGGTGAGAAGATTCTGCGTGGTCTTGCAGTTGGCATAGCTTTAGTA1580.5681205278486929No Hit
AAGTACGGGTTATCTATACAGTTTTTTCAGTGCTAAAATATTCTGGATAAAT1580.5681205278486929No Hit
ATTCGGGTAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGAT1580.5681205278486929No Hit
TACGCAGGGGACCTGGATGCTGAAGCTGCCATGGACATCTCAGAGGGCCGCT1530.5501420301319622No Hit
AATCCGGGAAGATTGATCTATATATTTGATATAATCACAATCAAAATCCCAG1490.5357592319585776No Hit
CCGATAGGGCATGCGGCCTAGCTCGGTTCCGCCTCCTCCTCGCGCGGCCCCA1490.5357592319585776No Hit
TGCATGGGGCGATCTTTAGTTGGGTGTTGACTTCATCTTCCTTAACTGTCAA1490.5357592319585776No Hit
ATGACAGGGTTGATCAGCAAATGGAACATTCTCAGCAGAGGGAAAATGAACA1460.524972133328539No Hit
CTTGTGGGAGAATTATTTCCTTTGTGTTCGTGAGTTGTTTTAATAGGAATTT1450.5213764337851928No Hit
ATACATGGGCAGTGGTGACAGGTCTAGCTTATTTCAAGAGCTGCACAAAAGC1400.5033979360684621No Hit
TCAAACGGGCAACTCAAAGAGAGCACACACCATGTCTGTTTTGTTCACTAAT1350.48541943835173135No Hit
AAAGTTGGGGAGAGCCGTCGTGGCTCGTTCCATTCTCGGCGGTGGTACCTGC1330.47822803926503904No Hit
GTTAATGGGATTCTTCTACTGATGAACACTTAGGTTGTTACCATATCTGGAC1330.47822803926503904No Hit
AACTCAGGGAACTGACATAGATTGTCAATTTTTAAAAAATTTTCAGATTGAT1310.47103664017834673No Hit
CTATTGGGGGTGGAAATTTGCAGAGGGGCAGGTGTGTGGTAAACGGGTAATG1280.46024954154830816No Hit
TAATGTGGGCAGAAAGGCCAGCGGGGAGAGGCTGGGGCGAGGGGAGGAGGGG1270.456653842004962No Hit
TCATAAGGGAATATATAAATATGGTGTCTGTGTACATATAGTGAAGATATGC1250.4494624429182697No Hit
GAATACGGGATTCAGTATTAACGTAGCAACATCATCATACTTTTAATTAGTA1230.4422710438315774No Hit
ATAGTGGGGACATCAGCTCTCAGTAACCCTAGAAGACTTATATAATGGTGCA1220.43867534428823124No Hit
TTACGAGGGGAATTGGTTCTTTCTCTGTAAAACAAATGTAACTATATTTATT1200.43148394520153893No Hit
CAATCGGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1190.4278882456581928No Hit
GATTTTGGGTAAGCTGATGAATAGTCCAAAAGAAGCCTCAGAATTTACTTAT1170.42069684657150047No Hit
GTACTTGGGAAACTCAACCTTGTACTCATATAATGAAGAGAATAACAAGAGT1150.41350544748480816No Hit
CTTCTAGGGGGTGGCACCGCTGGAGGGGGCGGCCTGGGGCTGGGGCTGCCCG1130.40631404839811586No Hit
GCTTATTGGGGAAGGCTGGCTTCCATGTGCAGCACTCTTCCCAATTGCCAGG1110.39912264931142355No Hit
ATTACCGGGGAGTGGTGCTGGGAGTGTCGTGGACGCCGTGCCGTTACTCGTA1090.39193125022473124No Hit
ACTTTGGGGGAATGTTTTTCTTATAAAAAATTATACAAATGCTGAAGTGAGA1060.3811441515946928No Hit
AGTGGCGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1060.3811441515946928No Hit
GAGTCAGGGGTGCTCATTCTCCCTAAATAGTAAATCCCACTGTATACAAAAC1060.3811441515946928No Hit
GGTAATGGGAACTCCTAATATTCTGGCTTTGTTCTACCTGTAAGCTTCTCTG1050.37754845205134663No Hit
AGACGGGGGACCTAATATCAGAGCTGCAAATACATCAAGCAAAAATTAATGA1030.37035705296465427No Hit
TTATAGGGATTCACTCCCAGTTGGATCATTTACTGGATCCTTCTTCTTTCAC1030.37035705296465427No Hit
GATTGGGGCTGTGGGCCATTGTGTTTATCTTGGGTAGACTTGTGACTTTAAT1020.3667613534213081No Hit
GGACACGGGGCCAGAAAAACCTGTTGATCAAACAAAGCTGTTTATTAGCTTA1020.3667613534213081No Hit
TGCCGCGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1020.3667613534213081No Hit
TTATATGGGGGGCTCTCGATGGTGGCGTGACGGGGGCGGGGGTGGCGGCGCG1020.3667613534213081No Hit
GTAGTAGGGAGGGAGCCGGAGGGGACGCGCCGGAGGAAAGATGGAAGACTAC1000.3595699543346158No Hit
ATTTAAGGGAATGTGTTTGGAGCACTAAATACGACTTGAGTGCTTGCTCATT990.35597425479126965No Hit
CGCAAGGGGTGACTAGTTGAGTGATTTGCCCCAAGTTATACAACTTTAAAAT990.35597425479126965No Hit
ACGCATAGGGATAATAGGGATTTGATAAGTGTTCATTACAGTTTAAAAAATG960.3451871561612312No Hit
GAATAGGGATAACAGTTTCAGTGAATAGAGAAAAAATTCTGAACTGATCATA960.3451871561612312No Hit
GCGACTGGGATTGAGTAGAGGCCTAAAGAGGACTGTGCATTTTTGCTATTTA960.3451871561612312No Hit
CATCGGGGGAGTAACTTTCATGACATAGGATTCTGCCGCCAAATTTATATCA950.34159145661788504No Hit
TGCATCGGGAGGATGAGCTATGGAAGGAGTGAACACTGGAGCAGGAGTATAG940.3379957570745389No Hit
CTAAAAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC940.3379957570745389No Hit
TAGCTCAGGGTACTGGCTGTGGCTGGAATCTGCCTTTTATCACAGCTGTCAC940.3379957570745389No Hit
ACACGACGGGCTTTCTAGATTTACTGTCTAAATTTAACCTTTCCAAAGCATT910.32720865844450037No Hit
CTTGTCAAGGGATAAATTAAAATCAGCAGATTGAGTTTTCAACATTGCAAAA910.32720865844450037No Hit
GTTTACGGGATACTAAACACTGTTTATGTAATAGCATAGTGCTGGTTTGAAT880.3164215598144619No Hit
GCTCATGGGATTGGGTTTCTTAGCTGTGCATGCAATTTGAATAATTACTACA880.3164215598144619No Hit
ACACTTGGGCAGCGGCCCGGGGAACCCGCTCCCAGACAGACTCGGAGAGATG860.3092301607277696No Hit
GACGTGGGGATTTTTTTAAAGTTACATTAAGATTTTTTCTCTTTTGCAGCTA860.3092301607277696No Hit
TCATTTGGGTATGACTGATAGCAGAATAATAATGCATCTTACTTCTATATAG840.3020387616410773No Hit
TACAGTAGGATCCTACTGTCAGCATTGGACAGTTCATCTAGACATAAAATCA840.3020387616410773No Hit
ATTGTTGGGAAATCATATTTCAACAAAGCTGTTTTAAGAAAAATCATATTTC830.2984430620977311No Hit
AGTAGCGGGGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAG830.2984430620977311No Hit
ACAAAAGGGTCGCGGGGGCAATTTAGGAATGCTCAATGTGCTGTTGATTCTT810.2912516630110388No Hit
ATGAGGGGTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGC800.2876559634676926No Hit
TTGCATGGGTATGTGATAATATGCTTAAAATGAAAGCAAGTTCCATATTTGT780.2804645643810003No Hit
CTGAGTGGGGGATGATTGTGGATCCCCTGAGATCTGGCTTCCCAAGAGCACT760.273273165294308No Hit
CAGTCTGGGGGGGAACATTCTCAAGAGGTGAAATACAGAAAGCCTTTTTTTC760.273273165294308No Hit
ACCTCGGGGTATTAATTTACTATGAAATGGACATCCTTTTAACTAAAATGGA740.26608176620761564No Hit
TGCAATGGGAAACAAAATATATGACAATAGCACAAATACCAAGAGGATAGAC740.26608176620761564No Hit
ATTCTAGGGGCATATATATATTTATACCACCATGGACTTACTTTCACACTGG720.25889036712092334No Hit
CAGCATGGGATATGAAGCCTGCATATACTGTGAGCTGTGATTGGGGAACACC720.25889036712092334No Hit
CTACTCAGGGGATCTTGCTATGGGGACGGTGATATTGGAAATTATTATTATG710.2552946675775772No Hit
CTGAAAGGGACACATATGAAAAGTACTGGCCATTTTACCAGAAGCAAGGAGG710.2552946675775772No Hit
TAGCGGGGACAAACAAATGTGAAGATATAATGTGTATTTTATTTGAATGTCT700.25169896803423103No Hit
CTAGCGGGGAGTGGGAGAGACCCTAATAACTATTTTGGTGAAGGATGAATCT680.24450756894753875No Hit
ATTTGGGGGCATGGATAATTATTTTAACAATGCTAATAGTTGAGTTTTGCAG660.23731616986084644No Hit
AAGCGTGGGCGATGACATGAAAGTGAAGTAAATTTATTCTATGTAAATTCAC630.22652907123080793No Hit
CTTTTAGGGATGCTTTTTCTGAATGGACAGGAGAAACATAAGCTACGGAGTA620.22293337168746177No Hit
CATTTGGAAGATAGAAAATGTATTAGAGTTCTTCAGAGAAGCAGAACCAATA610.21933767214411562No Hit
GCTACCCGGGGCACTATTCATCATTGTATCTTCACCATCTAGCACGGTGCTA610.21933767214411562No Hit
TGGCTGGGGAACAATGAAGAATGTGGTTCCCAATATCTTCAATTTTTCTCAT610.21933767214411562No Hit
GAATTAGGGATTGGAAGACTAAAGCAGCAAATATGTCTTCATTGACAAAAAT610.21933767214411562No Hit
AATAAGGGGAGATGTTGTTGATAGTCATCTTTAAACTGAGTTAAAAGATGCT600.21574197260076947No Hit
TGCAGTGGGATTGCTGCATTCATGCAAGACTGAAATTTATTATTAGACAAAT600.21574197260076947No Hit
TAATGGGATACTAGGAACATTTGAATTATTTTTTTCTAGTTATTTTTAAATA600.21574197260076947No Hit
GGCTTTGGGATGTTGACTGAATGACTATGAGGAAAAACAAGTATTATTAACA600.21574197260076947No Hit
TGTTCTGGGAGAACTCTCAATCCAGGGCTGTTTCCTCTATTTGTAGCTTAAT590.2121462730574233No Hit
AGCACAGGGATGAAAGACATAACTACTATCCCTCTTTATCAAGGTCTCATGT590.2121462730574233No Hit
AAGTTGGGGAGGCCCCAACGTTGTAGGCCCCTACGGGCTACTACAACCCTTC590.2121462730574233No Hit
TATGAGGGGCTGCGATGGCTGAAATGACAGAAATAAAATTTGGAATATGGAT580.20855057351407716No Hit
ATCGCCTAGGGACAAAAACACGTGGCCAGGTCAGGCCCTGTGTTTCCTGGCT580.20855057351407716No Hit
GAACCAGGGATAATCCATATTTGCTGTTCAAGTTAATCTAGAAATTTATTCA580.20855057351407716No Hit
ATGCTGGGGGCCCCACCCTTGACACGTGGGAATTATTACAATGCAAGGTGAG580.20855057351407716No Hit
GTTCGCGGGGAGTCTTACCTCGTTGTGGCGTCAGAACCGCCGTCGCTCGCTC570.204954873970731No Hit
TGCGCTGGGAGCCGTGGGGCCAATTTTTTAACTCAGATCTTGCTGAGACCAG570.204954873970731No Hit
TGTGCTTGGGTATAACAGCCTTTTCCTTCTGGTTCTAACTGCTGTTAAAATT550.1977634748840387No Hit
GTCTTTGGGTGCAAACAGTACCTAACAAACCCACAGGTCCTAAACTACCAAA540.19416777534069254No Hit
TCCTCAGGGGGAAAAGAAATTAGAATGTCTAAATAACATGTATCATCCAGGA540.19416777534069254No Hit
TTTTCAGGGACGGTTGTATTTTCTGATCATTGGTTTCTTAATATGGTTGTAC540.19416777534069254No Hit
TAGCGTGGGAGTACCTGAATTCTTTGGCTGTGAAAGCATATATTCTAGTACT520.18697637625400024No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCTTATAC520.18697637625400024No Hit
TATAAGGGGGATGAGTTTCTTAGAAAAATATAATTATAAAAATTTACTTAAA520.18697637625400024No Hit
GAACAGGGGAACTTGGTAACTTCTGGGTTTAGTAGAGCCTCAGTGTCGCTTT510.18338067671065406No Hit
AAATACGGGATGGATTAATGTAACAGAATACCTAGCTATTACTTTAGCAGAA490.17618927762396175No Hit
TGTATGGGGATATGTTTGAGGATTTTTCTTTTCCTTTTTAAATTTTTTTATT490.17618927762396175No Hit
ATTCTGGGGGGAAGGCACATTGTATCAATTCTACCTTGTGCTATACGTAGGA490.17618927762396175No Hit
GCACATGGGACGGGAACACAGCGCTGACCTACGCCCGGCAGGCCTCCAGCCA480.1725935780806156No Hit
CGCTCTGGGCAAGGAGATCATTCCGTGTATATATCTCCATCAATCATACTTC460.16540217899392326No Hit
GACGAGGGGCGTCTCCTGTGGCTGGGACAAGCTGGTCAAGGTATGGAACCTG460.16540217899392326No Hit
AATACAGGGTTTGCCATCTCTGCTCCAACCGGGATGGGAGCCGGGCAAACTG460.16540217899392326No Hit
TTTCTCGGGATGGAGATGTGTGTCCGAGGCTACCTGGCTGTGCGGCCCTACA450.1618064794505771No Hit
GTGGGTGGGATGTCACTGTCATTCATTACATTTTAATTTTTGAAACTGATGT450.1618064794505771No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTG450.1618064794505771No Hit
CGCTCAGGGTACGCGGGGGACATTTGAGTGCTTTCGTAAGCCCTGTACATGT430.1546150803638848No Hit
CTACGGGGTGTGCATCTGAGAGATGTCAAAGCTTTTCAGAGTCAAAATGAGA430.1546150803638848No Hit
TCGTCTGGGATCCCACTTGGTTTTTGACTGAAGGGGAAGTGTAGAAATATAT430.1546150803638848No Hit
TATGCAGGGCCTTGACATTTATTTGTTAATCATATGATCATTTTTTTTTCAC420.15101938082053865No Hit
GACACAGGGCTGAGCTGGACCTTAATGTATGAGTAGGATGTTGAAGAGTTGA410.14742368127719246No Hit
TAGCTTAGGGGGGAATTGAGAATTTATTTGAAATAGAATAGGTAACACCTCA410.14742368127719246No Hit
TAACGTGGGGGGGGCTCTCTTTGCTTCGAGAAGTAAACCTAATACCATTTTT410.14742368127719246No Hit
ATAGCTGGGATTGTACAGATTTGTCTGTATTTCTCACCATATCTAATGATAC410.14742368127719246No Hit
CTGCAAGGGATAGTCTTTCTTAGAACCATTTTTATGGTTTTCATAAAATTGC410.14742368127719246No Hit
TACACAGGGAATAATGCTCACTTCTGATTTGATGTTGATATACTTTTATGGT400.1438279817338463No Hit
ATGTATGGGATCTTAAAATGTTCCTTCTCACTTATTCTACTGGAATTGACCA400.1438279817338463No Hit
GCTGCTGGGATCTGTAAAAGGTTTCTCCCTACTGTCAAAAAAATATGCATGT400.1438279817338463No Hit
TACTAAGGGTATCACAGCATGGGGGCTGGCTCCCCTCGGAGTGGATGGCCTG400.1438279817338463No Hit
TGACCGAGGGAATGCCAAAATTCCATTGAAACTTTCAAGTTGGAGCAATTGT390.14023228219050016No Hit
CGCACAGGGACACGACGGCTCTTCCGATCTCAAGAGGGGTGCTCTTTTATTT390.14023228219050016No Hit
AGCTAAGGGAAGCCAATACCTTTCTCTCTCTTTCTCTCTTTTCTCTCTCTTA390.14023228219050016No Hit
TGCGCGGGGAGACCATGGTCCCTAAACCTGCAGTTTCTTCCTGCTGGTGCAG390.14023228219050016No Hit
AGGTTCGGGTCCACTTGTTTATTGTGGCACTTGCTGGAGCTGGGGCAGCAGT390.14023228219050016No Hit
GTATCAGGAAATGTAAGAATACTTTAAATCTCTAAGCTTCTGAAGTGTTAGA380.136636582647154No Hit
ATGCATGGGCTGTTGTGGCAATACTATGTGGTTTCTGTTACAACAATTATTA380.136636582647154No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGA380.136636582647154No Hit
TACCAGGGAGGTCTTTTAAATGAGTCATTTTCAGCTTTTTTAAACACTTAGT370.13304088310380782No Hit
CTTATAGGGACTTCACAGACTCAATGAGGCAGAAATTATTGTAGTTTTCTCC370.13304088310380782No Hit
AGCGCAGGGAGATGGGTGCTGGTCCTCAGGCGTTGGGGACACTGCTGGGTCG360.12944518356046167No Hit
CGGTTTGGGCCTCAGATGAAAAGGAGTTGGTCACTAAGGAATGACACACATC350.12584948401711551No Hit
AGCTTTGGGCTCCTTTTGCCAATACCACATAAGTCAAGATGACTATAGCTTT350.12584948401711551No Hit
CGCTAGGGAATCCTGAACATGTAGAAAAATTCCTAAACATAATCAGACCAAT350.12584948401711551No Hit
CCTTTAGGGAAGTAAAGTTTCCTGCGCACCTTATACCAGAATTCAGTATAAT340.12225378447376938No Hit
TTCACCGGCATACCTCTGCTTTATCTTTTGCATTTGATCATTCATATGAAAA340.12225378447376938No Hit
CTTAAAGGGTGGCAGATAACTTATATATATGAAAAAGTATAGTAAGAAATTT340.12225378447376938No Hit
GTAGTTGGGATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT340.12225378447376938No Hit
AAGCACGGGCAAGGGGATGATCCTCCTACTTCAGGAATTATGCCACCATGCC330.11865808493042322No Hit
AAGTTTGGGGGATTTAATCTAAAGAACAATAGTAAACAATAATTACAGGCCA330.11865808493042322No Hit
TGCCGTGGGGTTCCTTCCCATGTGCTTCTCCCCAAATTTTCATATAGATATA320.11506238538707704No Hit
TTGACGGGGAAGTCATGAAAGCTGCTAGATTTTTTTACAGATCTGGAAACTT310.11146668584373089No Hit
TGGTAGGGGTTTTTAGGATAGTGTAAATGGGTATTTAGATGATATCAAATTC310.11146668584373089No Hit
TACAGCGGGGTCCAGGTAAGAGGGCCTCACACAGGAGGGCTTGCTCAGAACC310.11146668584373089No Hit
TTTTATGGGCTCCAGGCCAGCATTGTTGGTGGTGATTGCTCTGGACCTCACT300.10787098630038473No Hit
TCATGGGGGAAGCCTGTGGTTGAGGTTTCCTTTAGTCTTTGGGAAAAGTATG300.10787098630038473No Hit
CTAATAGGGGTTTTAGATAGAAAGTGAGCCAGTCAGAGAGAGAGACTGACTT300.10787098630038473No Hit
CTGTTCGGGAGTTGGAGATTTTAACACTAATCTTTCAGTCATTGAGAGAATT290.10427528675703858No Hit
TCTAACGGGATTTATTTATTTATATAAACAAAAATATAGAGATCTTACCTTG290.10427528675703858No Hit
TCGCGTGGGAGCTGACAGCTTTTCAGGGGTGGTCTGAAAAGCCAGTTTGACA290.10427528675703858No Hit
CAAGTGGGAGTCCTTTGAGGAAGAGACGATGTGTCAGAACAAGAGGTTAGAA280.10067958721369243No Hit
GTTAAAGGGAAGCAGTACTTTTGGAACTGAGTAATAACATTTATTTGCCACT280.10067958721369243No Hit
GCCTATGGGCAGCACTCAGCTGCATGCGGGACAGGTGAGCCTGCAGGAGCAG280.10067958721369243No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)