FastQCFastQC Report
Sat 31 Dec 2022
SRR5845297.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845297.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14746
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC2101.4241150142411503No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT1801.2206700122067002Illumina PCR Primer Index 5 (95% over 21bp)
GACTGCGGGAATGGAAAGTGAAAATGCTGCATCTGAGAAAAGTCAAGGTTTG960.6510240065102401No Hit
TTGCTTGGGCCAGAAGTAAAACTGCTGGATCATATACTGGTAGTTCTGTTTT920.6238980062389801No Hit
TGCTCTAGGGGCTTCTCCACCAACGGACCCTCTGGGGACAAGAGTCTGAGCC870.5899905058999051No Hit
GCACATGGGGACCTTCATCCTTTGAATAACTCTTTAGGGGAGAGAAATGATG850.5764275057642751No Hit
TTGTACCGGGGTATGCTAAGTAATCTTTCATTTCTTACAGAAGACGATCACC830.562864505628645No Hit
TTTAGTGGGGGTTAAAATACAATTTAGTTTATCCATTTTCTTGTTGATGGGC800.5425200054252001No Hit
AGTCCCTGGGAAAGGCGGTGAACTTGAGTCTAAAGATGCAGTGCCTGGCCCT790.535738505357385No Hit
ATTGAGGGGGGGGGCGGAGCCTCTGGCGATGACAACAGCCACACGTGATCGG780.52895700528957No Hit
TAAGCTGGGTGACCTGAATCTCAACCCAAATATTAAACAAAATCCACATAAT780.52895700528957No Hit
TTAATGGGTGCTAAATGAGTATTACAACTGGCTAATATCATTTTTTATATAC780.52895700528957No Hit
TAAAGTGGGAGGAGTTGAATGAACCCCTGAAACAGTCTACCTCTTTCCTGAT770.522175505221755No Hit
TACGGTGGGACCATAAATAATCTATGCCTTATTCATATTGATTCAGTTAGTT770.522175505221755No Hit
TTTCATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA770.522175505221755No Hit
TAGTATGGGAGGTCAAAGAATGTTTTTTAAAAACACAAAGCTGCTGAATGTT750.5086125050861251No Hit
GACCATGGGAACATGGTCTAATTATTTACTCAAAGACATAGATGTGAAAATT730.49504950495049505No Hit
GCAAAGAGGGGTCATGGAGGAACTGCTAAAGATTGTGAAGAGCTTGCTCCAA720.4882680048826801No Hit
AAAATCGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC710.48148650481486505No Hit
GAGAACGGGGTGGTAGTATCTGAGCCCAAACTCTGAGACGCTCTAGAGTTTT690.46792350467923505No Hit
GAGCAGGGGATAAACAAGATTTTGATGAGTAATCTTGAACATGTAGCTACTA650.440797504407975No Hit
ATTGGCGGGGAGGCACATATTTTGAGGAGTAGCAAGTGGAATGGTATAATGA640.43401600434016No Hit
AATTCGGGGTTCTGGGACCCCTTTCCTTGCTCCGTGAGGCTCTGTGGCCATC640.43401600434016No Hit
TAGTTAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC640.43401600434016No Hit
GCCGATGGGGCAACAAGAAGACTGCTCTTGACTGATAACAGCTCTGTCAATA630.427234504272345No Hit
GTTGCAGGCAAGCTATAACCACAAAAATAATGAATTGATGAGAAATACAATG620.42045300420453No Hit
GACAGTGGGTTAGCAAAAATATATTCTGTTCATGTATATCCAAGAGCAAAAT600.4068900040689No Hit
TCTTCAGGGATTGTAGGCATTTTTGCCTCATATTTTACTGGGCCATGTTTGT590.400108504001085No Hit
ACACATGGGGAGTTAACATGAACCTTCATTTGTAAATTTTTTAAAATTTCTT590.400108504001085No Hit
TATCCCGGGGCGAACACAGTGACACGTTTGGCCAGTGACATTTCAATCACTG570.38654550386545505No Hit
CTCGGGGGTTCTTTGTAAACTGCCTTTCCCTGTTTTTCTGTTTTGTTTTGTT570.38654550386545505No Hit
GTATCAGGGTTCCACTTTCCATTTTCTCTTTCACCTCATTTTGCCTCTCTAT570.38654550386545505No Hit
GTAACTGGGGAAAGGAGATGTCCAGCCAATGATGCCCGGCTTCTGAGGCCCA560.37976400379764No Hit
GCACAAGGGTGGACAAAGGCTATAAAAATATTTTATAAATAAGAAATACAGA550.37298250372982505No Hit
CGACCTGGGTGGCCCAGCCATGGTTCGTGCCTCTGAACAGGACAGTGGCCGC550.37298250372982505No Hit
AGCACGGGGACTGCAGCTATCTAACATTTTATGAAACTGACGCATGTCCTTC550.37298250372982505No Hit
TTTATCGGGGATTTTGCCAATGGAATAACAGATTTGCCGGCATTCACTGAAA540.36620100366201No Hit
CTAAACGCGGGATGTAGAGCATATCTGTTATATGTTTATGTAACTATCAAAT530.35941950359419506No Hit
TGTGCTGGGCTTACCTGACTGGGGAACTGACGGCCACCAGTGAAGACTATAA520.35263800352638003No Hit
TGAATATGGGAGCCAGGTCTACCCCAAATAGAGCAGCAGCTGATGGCTTCTC520.35263800352638003No Hit
GCTACAGGGATTACTAATATTTTTTAGTAACTTCTGTTTACAGAGCCACTCA510.345856503458565No Hit
AAGGGGGGTCATCCAGTGGGTGTCCGACTTCAGCGAGAGGATCAAACAGCTG510.345856503458565No Hit
AAGACTGGGCGATCTAACGGAGGGATACTCATTATGGAAAAATCTAGAAAAA510.345856503458565No Hit
TTTAACGGGAGAGATGCACTTTATACAGATGTCACATTTAATCTGTAGAACA500.33907500339075003No Hit
AGTCATGGGTCAAGAAACAGGGACCCATTTGGACCGTCTTTGGACCCTTTTG500.33907500339075003No Hit
GTCTAGGGATCTAAATAGTGGAAATGCAAATGAGGCTGTCAATGGTGTAAGA490.332293503322935No Hit
AAATTAGGCACTCAGCCTGATGTTCCCTCTTTTATAAGTGCCATTCCTTCTT480.32551200325512003No Hit
GATGCCGGGGAGAGTGACCGCGGCTGCTCCAGCGCTGACGCCGAGCCATGGC480.32551200325512003No Hit
AACTAAGGTATAAGGAGCTAGAAGCATTATAGCTAGTGTTTGATTCACTAGT480.32551200325512003No Hit
GCAGAGTGGGTACTCTGGATTTCCCAGAGAGTCGGGTAATCAGTCAGCCAGA470.318730503187305No Hit
GTGATTGCCAGGAGTTTGAGGGGAAGAAAATAAAGGGATGAACAGGTGGACA470.318730503187305No Hit
ACACAGGGATATTTATAGAGTTATTATAGTTATTCAAATCCATAAGCAGGTT460.31194900311949003No Hit
CCTGCGGGGGTGCATATGCGTGAGTTTCATTGCCGGGGTTGGGGCACAATTG460.31194900311949003No Hit
GGGCTAGGGAAGAAGATGAGAGTACACGGTTGTTATGAACAAATGACTTAAT460.31194900311949003No Hit
CTATTAGGGGAGGGCAGGTTACATGGAAAGTTTATTTAATTGAATTAGACAT450.30516750305167506No Hit
CAGTAAGGGGAGGAGCTCAAGCTTGGGAAAATGGTGTGCATTCCTTGTATCG450.30516750305167506No Hit
TGCACAGGGCTGGGATATATGTACCATTGTCAGCTATGACTAAAAATTTGAA440.29838600298386003No Hit
CAGTTAGGGGTCCTCTTGGTCTTTGGAGAACATGTAGCTTGCCACAGTCTGA440.29838600298386003No Hit
CTACTGGGACGTGGGTGAGCGTGTGTGGGGGTACTAGAAGCTGATCTGATTG430.29160450291604506No Hit
ACTAGATGGGGAAGTTAATGTCAGCTTGCCCAGGTTCAAACTGGAAGAGAGT430.29160450291604506No Hit
AGTAAAGGGAAGAGAACTTTCTGAATTTGCAGCTAATCTCGGGGAAGAGAAA420.28482300284823003No Hit
CTCCTCGGGGGTGATGTGGGGATTTTGTCCCTGACTGCCCACTGTACAGGAC410.278041502780415No Hit
TAAATGGGGACATTCTCTCCCAGGTAACATAGTTCTCTCAATGTAAACTTGG400.27126000271260003No Hit
GTTTATGGGAATCGCTCATTGCATACTCTTCAATCAGCCACATAGCCCTCGT390.264478502644785No Hit
CTTCATGGGCCTCTATGCCATGGTGGTGGTTCTCTCGAGGGCCTCAAGATCC390.264478502644785No Hit
AAAAGGGGGGGAGAACGAAAGATGGCGGCGCCCGTAAGGCGGACGCTGTTAG370.250915502509155No Hit
AGTTACGGGCAAATGAATGTCACTTAATTTTAATAATGGTCCACACAACTAA370.250915502509155No Hit
GCTTTAGGGCTTTTGCACTGGTCTGCCCTTCCTAAATTAAGTATGCACTTCA370.250915502509155No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTG360.24413400244134004No Hit
CAGAAGGGGGTGGGTGACTATTTCCTGTTCTTCCCTGAAAGTCAGGCCAGTG350.23735250237352504No Hit
TGTGGCGGGATCCTAGGGACAGAAAAAGGCCAGTGTGCATGAGTAATGAACA350.23735250237352504No Hit
ATCTAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC350.23735250237352504No Hit
CCTTGCATTGCCCAGGAAGAAAGTAGAAGTGCAAAGTAACGTCAAACTCTGG350.23735250237352504No Hit
TTTGACGGGGCTCTTCCGATCTTGTAAGGGCCTATGAAGAGTAACAGTGTAG340.23057100230571004No Hit
CGATGAGGGGGGCACTGAGACGAAGCTCCCTGACAGCCGAGACTGGCAGCCC340.23057100230571004No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGA330.22378950223789504No Hit
GCCCAAGGGGTTGACACAGCAGGGCTAGTCCTGCATATTTTTTCTTAAATAT320.21700800217008No Hit
GATCATGGGTGCCAGGACCAAGGCTGAGCCCAGCTGTGGGAGTCCCGGAAGC310.210226502102265No Hit
TGCTTCGGGGTAGAAAATTTTTTGAAAAGTTATAATTACATTGAAAGTATAC300.20344500203445No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCT300.20344500203445No Hit
TGCAAAGGGATTTTTGTCATAATGAAACATTTGAAATTGGTAAATCTTAGAA300.20344500203445No Hit
TAGAGTGGGCGTTTATTATTTTACAGTAAATTTGAATAAATCTTAGGGGTCA290.196663501966635No Hit
TCTCGGGACAATGGCTGGGTTTGGGCAGTGGAGAGGGGACTTGGATAGGAGC290.196663501966635No Hit
GCGCTGGGGGTTTTTGCTTAGCATAAGGTTTTTGGCAGTTTTGGATCAATAA290.196663501966635No Hit
TGGCATGGGATTTCCGAAGTTGGAGTTCTCTTTGTAGGCCTTTCAAATACGT280.18988200189882No Hit
CAATTTGGGAAGACAGTCTACCTGCAGTCGGCCCTGAGCAGCTCTACTTCTG280.18988200189882No Hit
TAATCAGGGGCGCTGTCCATGGGCGTCCTTGGCCTGCGCCACCCAGAGATGA260.17631900176319001No Hit
TGCGTAGGGGGCGCCTGCGCAGTGTAGCCGGGTCAGCTGGACAGGGTCATCC250.16953750169537501No Hit
AAGTTTGGGATCATCATTCTTCTGACTCTAGATGGGACACTTGACAGTGACT250.16953750169537501No Hit
AAGCGAGGGATAATTGTGGTTTTTTTGAGACCTCTGCAGCCCTCAGATTCTG250.16953750169537501No Hit
TGTTACGGGCACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTA250.16953750169537501No Hit
ATTGTTGGGTGAGCCATGGTGTCGAGTGAAGAGTAGTTCTTGTTTGTTACAA240.16275600162756002No Hit
AGTTTTGGGCACATCACTGCATTCCAGCCTGGGCAGCAGAGCAAGACCCTGT240.16275600162756002No Hit
TCTAATGGGATTGTATAAAACAGTAGAATTCATGGAAGGGATAATTCTTTTG230.15597450155974502No Hit
GTTAAGGGGGCACTTCCTGAAGCTGAAGGTCAGCATAGACAAAGGGCCTCAG230.15597450155974502No Hit
ATAGTCGGGCACCATGGGATTAATATCCAATTGCTGCTTGTACACTCATTCA220.14919300149193002No Hit
TTCACTCGGGACAGGAAATCTGTTAAAGTCTTAAAATGGAATGAGAATGTTG220.14919300149193002No Hit
GAAAGTGGGAAGAGCATTAAGCTCTGAGTCCAGTGAGGTCATAGCAGTGTAT220.14919300149193002No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCTTATAC210.14241150142411502No Hit
AGCATAGGGATGATTACTCTGATTCTGGGTATGAAAAAAAATGGGACTCTGG210.14241150142411502No Hit
GGAAAAGGGTAATATTAATTAAAAAGGAAAAAGGAAATTGACTGAAACAGAA210.14241150142411502No Hit
TCGTTGGGGGAATTTTCTCTCTCCTGGCACGAATATAAAGCACATCTCTTAA210.14241150142411502No Hit
GTGGTTGGGGACAAGGCCCATAGGTATGGCCCCCCCCTTCCCCCTCCCAGTA210.14241150142411502No Hit
TTGCAAGGGGGCTGGTATCAAAACTTACCTATCAAGGAAAGTGATGACTGCA200.13563000135630002No Hit
TCTTAGGGGGTACCTGCAGGCCTGTGGGCTTGTACAGTAGATAATTAATTTC200.13563000135630002No Hit
CGTACTGGGTATGCGAGGGGTGGTCTAAAGTGTTTAGCTGTCAAGCCGGATG200.13563000135630002No Hit
AAATGAGGGAAGGGCAGAAACGGTTCACATTCCATTATTTGTAAAGTTACCT190.12884850128848502No Hit
CGATATGGGGACGCTCTTCCGATCTCAGCTGGGGATCTTTCCTCCTGTCTTA190.12884850128848502No Hit
TGTACTGGGAGGTGAAGGTGGAAAGTTGGCAGGAAAACAAGGTCAGCATCTT180.12206700122067002No Hit
CTCGCATGGGGAGCACCAGGTGGTCTCCTCTGACTTCAACAGCGACACCCAC180.12206700122067002No Hit
GGCATTGGCATAAAATAACAGTAAATTCCAAACCTCAAAAACTATTATGGCC180.12206700122067002No Hit
AGTAGAGGGGGATTCTTCTGGGCTGCTGGGAAGATCCTGGTGAGTCATAATG180.12206700122067002No Hit
CTACCGGGAGAACTTAATTGTCACCCAGCAAATAGGCTTGCCGGTTAAAGGT170.11528550115285502No Hit
TATCCTGGGATTTTGGTCACTTATATCAACAATCACGAATTTATATTTGTCA170.11528550115285502No Hit
TATAACGGGATTGAGGGAGGTGGAAAGCGCAGGAGGAAGCCAGTGAAGAGGA170.11528550115285502No Hit
ATACCGGGATTTCATATTCAGGATTAGATGATAAATAAAATTCCCAAATGCC170.11528550115285502No Hit
TAGGTAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA170.11528550115285502No Hit
GGCAGCGGGCCAAATTCCCAATTTTTTTTGGTCTTTTTAGGAAAGATGTGTT170.11528550115285502No Hit
CACCGTGGGGCGACCGGGGTTCAGCGCTCGGGTGAGGAGCTGGTGGCGTCGG170.11528550115285502No Hit
TCTTCTGGGGTGAGCTTATAGATAGAAACAGAATATCAACTTAATTATGAAA160.10850400108504No Hit
TAATATGGGTATCTCACTAACAGATTTGTGACTAAAAGTTAGGGATATTGAA160.10850400108504No Hit
AGAACGGGGTCCACGTTAGTTATGGGAATCCAGCAAGAAAACAGACAAATCA160.10850400108504No Hit
GGTCATGGGATTCTAAAATTAGGCCAGCAGTGGGGGCTGGGAGGGCATCTGT160.10850400108504No Hit
AGTTCAGGAACCATATTTTTCAAAATTAACGTTTTTGGAGGGAGAAAAATCC150.101722501017225No Hit
ATGCTAGGGGGGCAACAAACCTGCACGCTGTGCACATGTACCCTAAAATTTA150.101722501017225No Hit
GCCTTGGGGGGTCACACCCGGAAGCAGGGGCCCGAGCGGAGCCGGCCGCGAT150.101722501017225No Hit
TTTGTGGGGCTCTTCTTCCTCGGCGCTGCCTACGGAGGTGGCAGCCATCTCC150.101722501017225No Hit
TTGTGCGGGAGCAGTCAGGGAAGCTGGAAGGGTGAGCATTCAGCTATAGAGA150.101722501017225No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)