FastQCFastQC Report
Sat 31 Dec 2022
SRR5845296.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845296.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14853
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC2221.494647545950313No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT1941.3061334410556789Illumina PCR Primer Index 5 (95% over 21bp)
TTTCATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1000.6732646603379789No Hit
TGCTCTAGGGGCTTCTCCACCAACGGACCCTCTGGGGACAAGAGTCTGAGCC910.6126708409075607No Hit
TAGTATGGGAGGTCAAAGAATGTTTTTTAAAAACACAAAGCTGCTGAATGTT860.5790076078906619No Hit
GACCATGGGAACATGGTCTAATTATTTACTCAAAGACATAGATGTGAAAATT830.5588096680805225No Hit
TTTAGTGGGGGTTAAAATACAATTTAGTTTATCCATTTTCTTGTTGATGGGC780.5251464350636236No Hit
TTAATGGGTGCTAAATGAGTATTACAACTGGCTAATATCATTTTTTATATAC760.511681141856864No Hit
GACTGCGGGAATGGAAAGTGAAAATGCTGCATCTGAGAAAAGTCAAGGTTTG750.5049484952534842No Hit
ATTGAGGGGGGGGGCGGAGCCTCTGGCGATGACAACAGCCACACGTGATCGG740.4982158486501044No Hit
TAAGCTGGGTGACCTGAATCTCAACCCAAATATTAAACAAAATCCACATAAT730.4914832020467246No Hit
GTTGCAGGCAAGCTATAACCACAAAAATAATGAATTGATGAGAAATACAATG720.48475055544334483No Hit
GAGAACGGGGTGGTAGTATCTGAGCCCAAACTCTGAGACGCTCTAGAGTTTT710.47801790883996503No Hit
TTGCTTGGGCCAGAAGTAAAACTGCTGGATCATATACTGGTAGTTCTGTTTT710.47801790883996503No Hit
TGAATATGGGAGCCAGGTCTACCCCAAATAGAGCAGCAGCTGATGGCTTCTC710.47801790883996503No Hit
GCCGATGGGGCAACAAGAAGACTGCTCTTGACTGATAACAGCTCTGTCAATA710.47801790883996503No Hit
TTGTACCGGGGTATGCTAAGTAATCTTTCATTTCTTACAGAAGACGATCACC680.4578199690298256No Hit
GCTACAGGGATTACTAATATTTTTTAGTAACTTCTGTTTACAGAGCCACTCA680.4578199690298256No Hit
GTATCAGGGTTCCACTTTCCATTTTCTCTTTCACCTCATTTTGCCTCTCTAT680.4578199690298256No Hit
TACGGTGGGACCATAAATAATCTATGCCTTATTCATATTGATTCAGTTAGTT670.4510873224264458No Hit
GCACATGGGGACCTTCATCCTTTGAATAACTCTTTAGGGGAGAGAAATGATG660.44435467582306604No Hit
AGTCATGGGTCAAGAAACAGGGACCCATTTGGACCGTCTTTGGACCCTTTTG650.43762202921968624No Hit
TCTTCAGGGATTGTAGGCATTTTTGCCTCATATTTTACTGGGCCATGTTTGT640.43088938261630644No Hit
GCAGAGTGGGTACTCTGGATTTCCCAGAGAGTCGGGTAATCAGTCAGCCAGA640.43088938261630644No Hit
GCAAAGAGGGGTCATGGAGGAACTGCTAAAGATTGTGAAGAGCTTGCTCCAA640.43088938261630644No Hit
TATCCCGGGGCGAACACAGTGACACGTTTGGCCAGTGACATTTCAATCACTG630.42415673601292664No Hit
GTCTAGGGATCTAAATAGTGGAAATGCAAATGAGGCTGTCAATGGTGTAAGA630.42415673601292664No Hit
AGTCCCTGGGAAAGGCGGTGAACTTGAGTCTAAAGATGCAGTGCCTGGCCCT630.42415673601292664No Hit
GTGATTGCCAGGAGTTTGAGGGGAAGAAAATAAAGGGATGAACAGGTGGACA630.42415673601292664No Hit
TAGTTAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC620.4174240894095469No Hit
GAGCAGGGGATAAACAAGATTTTGATGAGTAATCTTGAACATGTAGCTACTA620.4174240894095469No Hit
AATTCGGGGTTCTGGGACCCCTTTCCTTGCTCCGTGAGGCTCTGTGGCCATC600.4039587962027873No Hit
AAAATCGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC600.4039587962027873No Hit
TAAAGTGGGAGGAGTTGAATGAACCCCTGAAACAGTCTACCTCTTTCCTGAT580.39049350299602775No Hit
AAGGGGGGTCATCCAGTGGGTGTCCGACTTCAGCGAGAGGATCAAACAGCTG580.39049350299602775No Hit
TGTGGCGGGATCCTAGGGACAGAAAAAGGCCAGTGTGCATGAGTAATGAACA570.38376085639264795No Hit
GCACAAGGGTGGACAAAGGCTATAAAAATATTTTATAAATAAGAAATACAGA570.38376085639264795No Hit
TGTGCTGGGCTTACCTGACTGGGGAACTGACGGCCACCAGTGAAGACTATAA570.38376085639264795No Hit
GACAGTGGGTTAGCAAAAATATATTCTGTTCATGTATATCCAAGAGCAAAAT560.37702820978926815No Hit
AGCACGGGGACTGCAGCTATCTAACATTTTATGAAACTGACGCATGTCCTTC560.37702820978926815No Hit
TTTATCGGGGATTTTGCCAATGGAATAACAGATTTGCCGGCATTCACTGAAA560.37702820978926815No Hit
ATTGGCGGGGAGGCACATATTTTGAGGAGTAGCAAGTGGAATGGTATAATGA550.37029556318588835No Hit
AAATTAGGCACTCAGCCTGATGTTCCCTCTTTTATAAGTGCCATTCCTTCTT550.37029556318588835No Hit
CTACTGGGACGTGGGTGAGCGTGTGTGGGGGTACTAGAAGCTGATCTGATTG530.3568302699791288No Hit
GCCCAAGGGGTTGACACAGCAGGGCTAGTCCTGCATATTTTTTCTTAAATAT520.350097623375749No Hit
TGCACAGGGCTGGGATATATGTACCATTGTCAGCTATGACTAAAAATTTGAA510.3433649767723692No Hit
CGACCTGGGTGGCCCAGCCATGGTTCGTGCCTCTGAACAGGACAGTGGCCGC500.33663233016898947No Hit
GATGCCGGGGAGAGTGACCGCGGCTGCTCCAGCGCTGACGCCGAGCCATGGC500.33663233016898947No Hit
CCTGCGGGGGTGCATATGCGTGAGTTTCATTGCCGGGGTTGGGGCACAATTG500.33663233016898947No Hit
ACACATGGGGAGTTAACATGAACCTTCATTTGTAAATTTTTTAAAATTTCTT500.33663233016898947No Hit
AAGACTGGGCGATCTAACGGAGGGATACTCATTATGGAAAAATCTAGAAAAA480.3231670369622298No Hit
GCTTTAGGGCTTTTGCACTGGTCTGCCCTTCCTAAATTAAGTATGCACTTCA470.31643439035885007No Hit
CTAAACGCGGGATGTAGAGCATATCTGTTATATGTTTATGTAACTATCAAAT450.30296909715209047No Hit
AGTAAAGGGAAGAGAACTTTCTGAATTTGCAGCTAATCTCGGGGAAGAGAAA450.30296909715209047No Hit
TTTAACGGGAGAGATGCACTTTATACAGATGTCACATTTAATCTGTAGAACA440.2962364505487107No Hit
TAAATGGGGACATTCTCTCCCAGGTAACATAGTTCTCTCAATGTAAACTTGG440.2962364505487107No Hit
ACACAGGGATATTTATAGAGTTATTATAGTTATTCAAATCCATAAGCAGGTT420.28277115734195113No Hit
CTCGGGGGTTCTTTGTAAACTGCCTTTCCCTGTTTTTCTGTTTTGTTTTGTT420.28277115734195113No Hit
ACTAGATGGGGAAGTTAATGTCAGCTTGCCCAGGTTCAAACTGGAAGAGAGT420.28277115734195113No Hit
CCTTGCATTGCCCAGGAAGAAAGTAGAAGTGCAAAGTAACGTCAAACTCTGG420.28277115734195113No Hit
GGAAAAGGGTAATATTAATTAAAAAGGAAAAAGGAAATTGACTGAAACAGAA410.27603851073857133No Hit
TCTCGGGACAATGGCTGGGTTTGGGCAGTGGAGAGGGGACTTGGATAGGAGC400.26930586413519153No Hit
CAGTTAGGGGTCCTCTTGGTCTTTGGAGAACATGTAGCTTGCCACAGTCTGA400.26930586413519153No Hit
CTATTAGGGGAGGGCAGGTTACATGGAAAGTTTATTTAATTGAATTAGACAT390.2625732175318118No Hit
AACTAAGGTATAAGGAGCTAGAAGCATTATAGCTAGTGTTTGATTCACTAGT390.2625732175318118No Hit
CGATGAGGGGGGCACTGAGACGAAGCTCCCTGACAGCCGAGACTGGCAGCCC390.2625732175318118No Hit
TAATCAGGGGCGCTGTCCATGGGCGTCCTTGGCCTGCGCCACCCAGAGATGA390.2625732175318118No Hit
TGGCATGGGATTTCCGAAGTTGGAGTTCTCTTTGTAGGCCTTTCAAATACGT380.255840570928432No Hit
GTAACTGGGGAAAGGAGATGTCCAGCCAATGATGCCCGGCTTCTGAGGCCCA380.255840570928432No Hit
CTCCTCGGGGGTGATGTGGGGATTTTGTCCCTGACTGCCCACTGTACAGGAC380.255840570928432No Hit
ATCTAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC380.255840570928432No Hit
AAAAGGGGGGGAGAACGAAAGATGGCGGCGCCCGTAAGGCGGACGCTGTTAG370.2491079243250522No Hit
GTGGTTGGGGACAAGGCCCATAGGTATGGCCCCCCCCTTCCCCCTCCCAGTA370.2491079243250522No Hit
TGCAAAGGGATTTTTGTCATAATGAAACATTTGAAATTGGTAAATCTTAGAA370.2491079243250522No Hit
TTTGACGGGGCTCTTCCGATCTTGTAAGGGCCTATGAAGAGTAACAGTGTAG360.24237527772167242No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTG360.24237527772167242No Hit
GGGCTAGGGAAGAAGATGAGAGTACACGGTTGTTATGAACAAATGACTTAAT350.23564263111829262No Hit
CAGAAGGGGGTGGGTGACTATTTCCTGTTCTTCCCTGAAAGTCAGGCCAGTG340.2289099845149128No Hit
TGCGTAGGGGGCGCCTGCGCAGTGTAGCCGGGTCAGCTGGACAGGGTCATCC330.22217733791153302No Hit
GTTTATGGGAATCGCTCATTGCATACTCTTCAATCAGCCACATAGCCCTCGT320.21544469130815322No Hit
CAATTTGGGAAGACAGTCTACCTGCAGTCGGCCCTGAGCAGCTCTACTTCTG320.21544469130815322No Hit
GATCATGGGTGCCAGGACCAAGGCTGAGCCCAGCTGTGGGAGTCCCGGAAGC310.20871204470477345No Hit
CAGTAAGGGGAGGAGCTCAAGCTTGGGAAAATGGTGTGCATTCCTTGTATCG300.20197939810139365No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCT300.20197939810139365No Hit
AAGTTTGGGATCATCATTCTTCTGACTCTAGATGGGACACTTGACAGTGACT300.20197939810139365No Hit
AAGCGAGGGATAATTGTGGTTTTTTTGAGACCTCTGCAGCCCTCAGATTCTG300.20197939810139365No Hit
TAATATGGGTATCTCACTAACAGATTTGTGACTAAAAGTTAGGGATATTGAA300.20197939810139365No Hit
CTTCATGGGCCTCTATGCCATGGTGGTGGTTCTCTCGAGGGCCTCAAGATCC290.19524675149801388No Hit
TATCCTGGGATTTTGGTCACTTATATCAACAATCACGAATTTATATTTGTCA270.1817814582912543No Hit
ATAGTCGGGCACCATGGGATTAATATCCAATTGCTGCTTGTACACTCATTCA260.1750488116878745No Hit
CGTACTGGGTATGCGAGGGGTGGTCTAAAGTGTTTAGCTGTCAAGCCGGATG260.1750488116878745No Hit
TAGAGTGGGCGTTTATTATTTTACAGTAAATTTGAATAAATCTTAGGGGTCA260.1750488116878745No Hit
CGATATGGGGACGCTCTTCCGATCTCAGCTGGGGATCTTTCCTCCTGTCTTA250.16831616508449473No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCTTATAC240.1615835184811149No Hit
AGTTACGGGCAAATGAATGTCACTTAATTTTAATAATGGTCCACACAACTAA240.1615835184811149No Hit
GCGCTGGGGGTTTTTGCTTAGCATAAGGTTTTTGGCAGTTTTGGATCAATAA240.1615835184811149No Hit
TTCACTCGGGACAGGAAATCTGTTAAAGTCTTAAAATGGAATGAGAATGTTG230.15485087187773514No Hit
TTTGTGGGGCTCTTCTTCCTCGGCGCTGCCTACGGAGGTGGCAGCCATCTCC230.15485087187773514No Hit
TGCTTCGGGGTAGAAAATTTTTTGAAAAGTTATAATTACATTGAAAGTATAC210.14138557867097556No Hit
TTGTGCGGGAGCAGTCAGGGAAGCTGGAAGGGTGAGCATTCAGCTATAGAGA210.14138557867097556No Hit
TTACCTGGGATCTCAGAGTTGGCATCCATTGTCTTTTTCATTCAGTTTGAGA200.13465293206759577No Hit
GTCTGAGGGGGGGGTGGGGGGGCGGCCCTGGGATAGGGGCTGTGGCAGTACG200.13465293206759577No Hit
TATAACGGGATTGAGGGAGGTGGAAAGCGCAGGAGGAAGCCAGTGAAGAGGA200.13465293206759577No Hit
TCTAATGGGATTGTATAAAACAGTAGAATTCATGGAAGGGATAATTCTTTTG200.13465293206759577No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGA200.13465293206759577No Hit
TGTTACGGGCACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTA200.13465293206759577No Hit
AATGCTGGGAGACAGGTATAGAGTGCTTGGTACAGAGCATGGCACATGGTAA190.127920285464216No Hit
AAATGAGGGAAGGGCAGAAACGGTTCACATTCCATTATTTGTAAAGTTACCT190.127920285464216No Hit
GCCTTGGGGTAGCTGTGTTATGTGCTGGTATCTTGAGAGTTTTGCCAAGAAA190.127920285464216No Hit
TTGCAAGGGGGCTGGTATCAAAACTTACCTATCAAGGAAAGTGATGACTGCA180.12118763886083621No Hit
ATGCTAGGGGGGCAACAAACCTGCACGCTGTGCACATGTACCCTAAAATTTA180.12118763886083621No Hit
GTATAGGGTAGGGAAAAGATGCACTAGAAAAACAGTACAAGTAATGACCACA170.1144549922574564No Hit
GGAGTTGGGAGAGTTCTTTGGAAAGAGTGGGGCAGTTGCTGAAAAGGCAACA170.1144549922574564No Hit
ATACCTGGGATTTTCTTTAGTGTAGTATGTGAAGGCACTGGTTCAACAGGAT170.1144549922574564No Hit
ATATACGGGATGTGGATTCTAACTCATTTTAACAAGGTAGCCTGACCTGCAT170.1144549922574564No Hit
ATAGCAGGGATAGCTCGCGGGGTACGGGCGCGCGTGCGCACTCCGCAGCCCG170.1144549922574564No Hit
ATCATCGGGAATGAAACTATCTACAATTCTTGTTTTAGCACATCTGTTATCC160.10772234565407661No Hit
CTACCGGGAGAACTTAATTGTCACCCAGCAAATAGGCTTGCCGGTTAAAGGT160.10772234565407661No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGAACTCGTATGC160.10772234565407661No Hit
GTATCAGGGGGAAGCGCGCATGCGCGCTTCGTCACAAGGGTGCGAGGAAAGT160.10772234565407661No Hit
GAAAGTGGGAAGAGCATTAAGCTCTGAGTCCAGTGAGGTCATAGCAGTGTAT160.10772234565407661No Hit
AGTTTTGGGCACATCACTGCATTCCAGCCTGGGCAGCAGAGCAAGACCCTGT160.10772234565407661No Hit
GTTTATGGGGGTTCCCAGGACGCTCTCATAGCACATTACCCAAGAATCTACA150.10098969905069682No Hit
TTCGCAAGGGACATTCTGAGTTCCCATTCTTCTTGGTTAATAAAAACTTTTT150.10098969905069682No Hit
TACAGATGGGATTTGAGGTCAAATTGAAGACGGAAACCGGAAACCGTTTTCT150.10098969905069682No Hit
CTACAGGTCCGTTTCCTTACACATTTTATCCCCTCAGAACACGATAAACCAT150.10098969905069682No Hit
GGCAGCGGGCCAAATTCCCAATTTTTTTTGGTCTTTTTAGGAAAGATGTGTT150.10098969905069682No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)