FastQCFastQC Report
Sat 31 Dec 2022
SRR5845295.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845295.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14692
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC2011.3680914783555675No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT1891.2864143751701607Illumina PCR Primer Index 5 (95% over 21bp)
TTTCATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1050.7146746528723115No Hit
TTTAGTGGGGGTTAAAATACAATTTAGTTTATCCATTTTCTTGTTGATGGGC930.6329975496869044No Hit
GCACATGGGGACCTTCATCCTTTGAATAACTCTTTAGGGGAGAGAAATGATG870.5921589980942009No Hit
TAAAGTGGGAGGAGTTGAATGAACCCCTGAAACAGTCTACCTCTTTCCTGAT860.5853525728287503No Hit
ATTGAGGGGGGGGGCGGAGCCTCTGGCGATGACAACAGCCACACGTGATCGG850.5785461475632998No Hit
TAGTATGGGAGGTCAAAGAATGTTTTTTAAAAACACAAAGCTGCTGAATGTT830.5649332970323986No Hit
TGCTCTAGGGGCTTCTCCACCAACGGACCCTCTGGGGACAAGAGTCTGAGCC820.558126871766948No Hit
TTGCTTGGGCCAGAAGTAAAACTGCTGGATCATATACTGGTAGTTCTGTTTT800.5445140212360468No Hit
TTGTACCGGGGTATGCTAAGTAATCTTTCATTTCTTACAGAAGACGATCACC790.5377075959705963No Hit
GACTGCGGGAATGGAAAGTGAAAATGCTGCATCTGAGAAAAGTCAAGGTTTG790.5377075959705963No Hit
GAGAACGGGGTGGTAGTATCTGAGCCCAAACTCTGAGACGCTCTAGAGTTTT740.5036754696433433No Hit
AAGGGGGGTCATCCAGTGGGTGTCCGACTTCAGCGAGAGGATCAAACAGCTG740.5036754696433433No Hit
TACGGTGGGACCATAAATAATCTATGCCTTATTCATATTGATTCAGTTAGTT710.48325619384699153No Hit
TAAGCTGGGTGACCTGAATCTCAACCCAAATATTAAACAAAATCCACATAAT710.48325619384699153No Hit
GTGATTGCCAGGAGTTTGAGGGGAAGAAAATAAAGGGATGAACAGGTGGACA710.48325619384699153No Hit
TTAATGGGTGCTAAATGAGTATTACAACTGGCTAATATCATTTTTTATATAC700.476449768581541No Hit
GACAGTGGGTTAGCAAAAATATATTCTGTTCATGTATATCCAAGAGCAAAAT680.4628369180506398No Hit
TGTGCTGGGCTTACCTGACTGGGGAACTGACGGCCACCAGTGAAGACTATAA680.4628369180506398No Hit
TTTATCGGGGATTTTGCCAATGGAATAACAGATTTGCCGGCATTCACTGAAA680.4628369180506398No Hit
TCTTCAGGGATTGTAGGCATTTTTGCCTCATATTTTACTGGGCCATGTTTGT670.4560304927851892No Hit
GAGCAGGGGATAAACAAGATTTTGATGAGTAATCTTGAACATGTAGCTACTA670.4560304927851892No Hit
GACCATGGGAACATGGTCTAATTATTTACTCAAAGACATAGATGTGAAAATT650.44241764225428803No Hit
AGTCATGGGTCAAGAAACAGGGACCCATTTGGACCGTCTTTGGACCCTTTTG640.4356112169888375No Hit
CAGTTAGGGGTCCTCTTGGTCTTTGGAGAACATGTAGCTTGCCACAGTCTGA640.4356112169888375No Hit
AAGACTGGGCGATCTAACGGAGGGATACTCATTATGGAAAAATCTAGAAAAA620.4219983664579363No Hit
GTATCAGGGTTCCACTTTCCATTTTCTCTTTCACCTCATTTTGCCTCTCTAT610.41519194119248565No Hit
GCCGATGGGGCAACAAGAAGACTGCTCTTGACTGATAACAGCTCTGTCAATA600.4083855159270351No Hit
GCAAAGAGGGGTCATGGAGGAACTGCTAAAGATTGTGAAGAGCTTGCTCCAA590.4015790906615846No Hit
GTTGCAGGCAAGCTATAACCACAAAAATAATGAATTGATGAGAAATACAATG580.3947726653961339No Hit
AAAATCGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC560.38115981486523276No Hit
TAGTTAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC550.3743533895997822No Hit
AGCACGGGGACTGCAGCTATCTAACATTTTATGAAACTGACGCATGTCCTTC550.3743533895997822No Hit
GCTACAGGGATTACTAATATTTTTTAGTAACTTCTGTTTACAGAGCCACTCA540.3675469643343316No Hit
TAAATGGGGACATTCTCTCCCAGGTAACATAGTTCTCTCAATGTAAACTTGG530.360740539068881No Hit
AGTCCCTGGGAAAGGCGGTGAACTTGAGTCTAAAGATGCAGTGCCTGGCCCT520.3539341138034305No Hit
ACACATGGGGAGTTAACATGAACCTTCATTTGTAAATTTTTTAAAATTTCTT510.3471276885379799No Hit
AATTCGGGGTTCTGGGACCCCTTTCCTTGCTCCGTGAGGCTCTGTGGCCATC500.34032126327252926No Hit
GTCTAGGGATCTAAATAGTGGAAATGCAAATGAGGCTGTCAATGGTGTAAGA500.34032126327252926No Hit
CTAAACGCGGGATGTAGAGCATATCTGTTATATGTTTATGTAACTATCAAAT500.34032126327252926No Hit
CTCCTCGGGGGTGATGTGGGGATTTTGTCCCTGACTGCCCACTGTACAGGAC500.34032126327252926No Hit
CCTGCGGGGGTGCATATGCGTGAGTTTCATTGCCGGGGTTGGGGCACAATTG500.34032126327252926No Hit
TGAATATGGGAGCCAGGTCTACCCCAAATAGAGCAGCAGCTGATGGCTTCTC500.34032126327252926No Hit
TATCCCGGGGCGAACACAGTGACACGTTTGGCCAGTGACATTTCAATCACTG490.3335148380070787No Hit
ATTGGCGGGGAGGCACATATTTTGAGGAGTAGCAAGTGGAATGGTATAATGA480.3267084127416281No Hit
TTTAACGGGAGAGATGCACTTTATACAGATGTCACATTTAATCTGTAGAACA480.3267084127416281No Hit
TGTGGCGGGATCCTAGGGACAGAAAAAGGCCAGTGTGCATGAGTAATGAACA470.31990198747617754No Hit
CTCGGGGGTTCTTTGTAAACTGCCTTTCCCTGTTTTTCTGTTTTGTTTTGTT470.31990198747617754No Hit
GCTTTAGGGCTTTTGCACTGGTCTGCCCTTCCTAAATTAAGTATGCACTTCA470.31990198747617754No Hit
CTATTAGGGGAGGGCAGGTTACATGGAAAGTTTATTTAATTGAATTAGACAT460.31309556221072693No Hit
GTAACTGGGGAAAGGAGATGTCCAGCCAATGATGCCCGGCTTCTGAGGCCCA460.31309556221072693No Hit
GCAGAGTGGGTACTCTGGATTTCCCAGAGAGTCGGGTAATCAGTCAGCCAGA450.3062891369452763No Hit
GCACAAGGGTGGACAAAGGCTATAAAAATATTTTATAAATAAGAAATACAGA440.29948271167982576No Hit
GCCCAAGGGGTTGACACAGCAGGGCTAGTCCTGCATATTTTTTCTTAAATAT440.29948271167982576No Hit
CAGTAAGGGGAGGAGCTCAAGCTTGGGAAAATGGTGTGCATTCCTTGTATCG440.29948271167982576No Hit
GATGCCGGGGAGAGTGACCGCGGCTGCTCCAGCGCTGACGCCGAGCCATGGC440.29948271167982576No Hit
AACTAAGGTATAAGGAGCTAGAAGCATTATAGCTAGTGTTTGATTCACTAGT440.29948271167982576No Hit
AAATTAGGCACTCAGCCTGATGTTCCCTCTTTTATAAGTGCCATTCCTTCTT430.29267628641437515No Hit
TGCACAGGGCTGGGATATATGTACCATTGTCAGCTATGACTAAAAATTTGAA430.29267628641437515No Hit
CAGAAGGGGGTGGGTGACTATTTCCTGTTCTTCCCTGAAAGTCAGGCCAGTG410.279063435883474No Hit
CCTTGCATTGCCCAGGAAGAAAGTAGAAGTGCAAAGTAACGTCAAACTCTGG410.279063435883474No Hit
ACTAGATGGGGAAGTTAATGTCAGCTTGCCCAGGTTCAAACTGGAAGAGAGT400.2722570106180234No Hit
ACACAGGGATATTTATAGAGTTATTATAGTTATTCAAATCCATAAGCAGGTT390.2654505853525728No Hit
AGTAAAGGGAAGAGAACTTTCTGAATTTGCAGCTAATCTCGGGGAAGAGAAA390.2654505853525728No Hit
CTACTGGGACGTGGGTGAGCGTGTGTGGGGGTACTAGAAGCTGATCTGATTG380.2586441600871222No Hit
GCGCTGGGGGTTTTTGCTTAGCATAAGGTTTTTGGCAGTTTTGGATCAATAA380.2586441600871222No Hit
TAGAGTGGGCGTTTATTATTTTACAGTAAATTTGAATAAATCTTAGGGGTCA370.25183773482167165No Hit
GTGGTTGGGGACAAGGCCCATAGGTATGGCCCCCCCCTTCCCCCTCCCAGTA370.25183773482167165No Hit
GGGCTAGGGAAGAAGATGAGAGTACACGGTTGTTATGAACAAATGACTTAAT360.2450313095562211No Hit
TGCTTCGGGGTAGAAAATTTTTTGAAAAGTTATAATTACATTGAAAGTATAC350.2382248842907705No Hit
CAATTTGGGAAGACAGTCTACCTGCAGTCGGCCCTGAGCAGCTCTACTTCTG350.2382248842907705No Hit
TGGCATGGGATTTCCGAAGTTGGAGTTCTCTTTGTAGGCCTTTCAAATACGT340.2314184590253199No Hit
CGACCTGGGTGGCCCAGCCATGGTTCGTGCCTCTGAACAGGACAGTGGCCGC340.2314184590253199No Hit
AAGTTTGGGATCATCATTCTTCTGACTCTAGATGGGACACTTGACAGTGACT340.2314184590253199No Hit
CGATGAGGGGGGCACTGAGACGAAGCTCCCTGACAGCCGAGACTGGCAGCCC340.2314184590253199No Hit
CTTCATGGGCCTCTATGCCATGGTGGTGGTTCTCTCGAGGGCCTCAAGATCC340.2314184590253199No Hit
ATCTAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC330.22461203375986932No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTG330.22461203375986932No Hit
TCTCGGGACAATGGCTGGGTTTGGGCAGTGGAGAGGGGACTTGGATAGGAGC320.21780560849441874No Hit
TGCAAAGGGATTTTTGTCATAATGAAACATTTGAAATTGGTAAATCTTAGAA320.21780560849441874No Hit
AGTTACGGGCAAATGAATGTCACTTAATTTTAATAATGGTCCACACAACTAA310.21099918322896816No Hit
TTTGACGGGGCTCTTCCGATCTTGTAAGGGCCTATGAAGAGTAACAGTGTAG310.21099918322896816No Hit
TATCCTGGGATTTTGGTCACTTATATCAACAATCACGAATTTATATTTGTCA310.21099918322896816No Hit
TTTGTGGGGCTCTTCTTCCTCGGCGCTGCCTACGGAGGTGGCAGCCATCTCC300.20419275796351755No Hit
TGTTACGGGCACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTA300.20419275796351755No Hit
TAATCAGGGGCGCTGTCCATGGGCGTCCTTGGCCTGCGCCACCCAGAGATGA300.20419275796351755No Hit
AAAAGGGGGGGAGAACGAAAGATGGCGGCGCCCGTAAGGCGGACGCTGTTAG290.19738633269806696No Hit
TGCGTAGGGGGCGCCTGCGCAGTGTAGCCGGGTCAGCTGGACAGGGTCATCC290.19738633269806696No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCT290.19738633269806696No Hit
TTCACTCGGGACAGGAAATCTGTTAAAGTCTTAAAATGGAATGAGAATGTTG280.19057990743261638No Hit
GGAAAAGGGTAATATTAATTAAAAAGGAAAAAGGAAATTGACTGAAACAGAA280.19057990743261638No Hit
GAAAGTGGGAAGAGCATTAAGCTCTGAGTCCAGTGAGGTCATAGCAGTGTAT280.19057990743261638No Hit
ATAGTCGGGCACCATGGGATTAATATCCAATTGCTGCTTGTACACTCATTCA270.1837734821671658No Hit
GTTTATGGGAATCGCTCATTGCATACTCTTCAATCAGCCACATAGCCCTCGT260.17696705690171524No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGA260.17696705690171524No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCTTATAC250.17016063163626463No Hit
GTTAAGGGGGCACTTCCTGAAGCTGAAGGTCAGCATAGACAAAGGGCCTCAG240.16335420637081405No Hit
TAATATGGGTATCTCACTAACAGATTTGTGACTAAAAGTTAGGGATATTGAA240.16335420637081405No Hit
GATCATGGGTGCCAGGACCAAGGCTGAGCCCAGCTGTGGGAGTCCCGGAAGC230.15654778110536346No Hit
CGTACTGGGTATGCGAGGGGTGGTCTAAAGTGTTTAGCTGTCAAGCCGGATG230.15654778110536346No Hit
TTGCAAGGGGGCTGGTATCAAAACTTACCTATCAAGGAAAGTGATGACTGCA210.1429349305744623No Hit
ATACCTGGGATTTTCTTTAGTGTAGTATGTGAAGGCACTGGTTCAACAGGAT210.1429349305744623No Hit
TCGTTGGGGGAATTTTCTCTCTCCTGGCACGAATATAAAGCACATCTCTTAA210.1429349305744623No Hit
GGCATTGGCATAAAATAACAGTAAATTCCAAACCTCAAAAACTATTATGGCC200.1361285053090117No Hit
TTGTTGGGATTACAGGTGTGAGCCACTGCACCTGCCCAAATATTCTTGTTAA200.1361285053090117No Hit
CTCGCATGGGGAGCACCAGGTGGTCTCCTCTGACTTCAACAGCGACACCCAC190.1293220800435611No Hit
TTATTGGGGTAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTA190.1293220800435611No Hit
TAGCAGGGATTGGAGGCCCCGGGATCTCTCGCAGTGTGGGGATTTGCTCAGT180.12251565477811055No Hit
TATAACGGGATTGAGGGAGGTGGAAAGCGCAGGAGGAAGCCAGTGAAGAGGA180.12251565477811055No Hit
TCTAATGGGATTGTATAAAACAGTAGAATTCATGGAAGGGATAATTCTTTTG180.12251565477811055No Hit
CGATATGGGGACGCTCTTCCGATCTCAGCTGGGGATCTTTCCTCCTGTCTTA180.12251565477811055No Hit
ATACCGGGATTTCATATTCAGGATTAGATGATAAATAAAATTCCCAAATGCC180.12251565477811055No Hit
CAACTCGGGTGCAAATGAGTAGTGAATGCTACACTCATTTTCAACTCTTTAA170.11570922951265995No Hit
TTACCTGGGATCTCAGAGTTGGCATCCATTGTCTTTTTCATTCAGTTTGAGA170.11570922951265995No Hit
TCTTAGGGGGTACCTGCAGGCCTGTGGGCTTGTACAGTAGATAATTAATTTC170.11570922951265995No Hit
AAATGAGGGAAGGGCAGAAACGGTTCACATTCCATTATTTGTAAAGTTACCT170.11570922951265995No Hit
CAAGCCGGGGATCTTAAGGTTTTTTGTAAGAAAAAAGAAAAACCAACAAAAA170.11570922951265995No Hit
GGGGTAGGGAATATAAGTAAATATAGATTATATATGTATAGTGTTTCACAAA160.10890280424720937No Hit
TTGTGCGGGAGCAGTCAGGGAAGCTGGAAGGGTGAGCATTCAGCTATAGAGA160.10890280424720937No Hit
TTTCACGGGCGTTTTAAAGGAGCATTGTAACCCCTATCCTCATCCCTACAAA160.10890280424720937No Hit
AAGCGAGGGATAATTGTGGTTTTTTTGAGACCTCTGCAGCCCTCAGATTCTG160.10890280424720937No Hit
CTCAGCGGGGAGCGCCGCGACGCGGAAGTGAGGTGCGTGCGGGCTGCAGCGC150.10209637898175877No Hit
CTACAGGTCCGTTTCCTTACACATTTTATCCCCTCAGAACACGATAAACCAT150.10209637898175877No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)