FastQCFastQC Report
Sat 31 Dec 2022
SRR5845294.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845294.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24342
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC770.3163256922192096No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT560.23005504888669787Illumina PCR Primer Index 5 (95% over 21bp)
AATGTTGGGACAGAAACCTAGCCATTCAAAATCTGTTTAAAGGACAAAGTTT500.2054062936488374No Hit
GCTGTTGGGTAAGATGTATATTAAAGTAAAGCTACATTTTTTTACTTCATTA490.20129816777586068No Hit
GAACATGGGATTCCATGCTTTTCTGTGTTTATAAATCTAGGGTTACATGATG450.18486566428395365No Hit
GTAACCGGGGGGACACACATACTTTTAGAAGTTAATAGAAGTCTTGAAGTCT430.17664941253800018No Hit
TCTACAGGGGTATTTACATATATTTTATATTAAGTTTCACATGAAATTATTC420.1725412866650234No Hit
GCGCAGGGATTGAAATCTGCAGTGAGATATAACTTTCTAGGAACAACAAAAT410.16843316079204668No Hit
AACATGGGGAGAGTGTGGTCAGGCGGCTCGGACTGAGCAGGACTTTCCTTAT410.16843316079204668No Hit
TTACGTGGGGAAGATGGAGGAGGAGATGGGATCAGAAACATAGCTGGATAAA390.16021690904609318No Hit
TTCCATGGGAGAGCTGAGTGTCACTATTGCAGTTGGAGCATCACTGCTGTTT380.15610878317311644No Hit
CGTAGCGGGACCACATACAATTTTTTACACAAAATTATTTCCTTCTAAATTC380.15610878317311644No Hit
CATTGAGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA370.15200065730013967No Hit
ATCAATGGGGGAGAGGTTGGCTTTTCCTGAATTATTCATATATAACTAAAAT370.15200065730013967No Hit
GGGCCGGGGATCTCAAGAAAAGAAGAAGTTCACAGATGAATTTCTAAAATCT370.15200065730013967No Hit
TTTTCAGGGATTTAGTAGGAATGTAAAGATACAAGTAGACAGAAAGTCTTCT370.15200065730013967No Hit
GCTCTTGGGAAGGATAGAGTGATGACATTTATTTTCATTCACTTTGAATCTG360.1478925314271629No Hit
CAGGCGGGGTGATTTAAGAAGTGGCTGGATTGGAACTTTTAATATGCTAATG360.1478925314271629No Hit
GCACAGGGGGGGCGAGCGACCGAGCGCCGCGACGCGGAAGTGAGGTGCGTGC360.1478925314271629No Hit
AACTAAGGGGACTTGAAATGTTAATGGAGAGATATTCACAACTCCAGTGTAA350.1437844055541862No Hit
TTAGGTGGGGGAAAGTGAGGCTTACCAATCACAACTTCTAGTAAATTAAAAG350.1437844055541862No Hit
ATTCATGGGAGTTTATTAAGATAGCTATGGACAGTGTAGGGGCTTAGTCTTG350.1437844055541862No Hit
CTCAAAAGAAGACATTTATGCAGCCAACAGACACATGAAAAAATGCTCATCA340.13967627968120944No Hit
TATAATGGGGACACACAACTTATTCTTCTGATGTTCAATATATGCTGATATT340.13967627968120944No Hit
GACAGTGGTGTCCCGGTGGTAATTGAAGGAAAACAGTTGTAATAGTAATAGC340.13967627968120944No Hit
GACTTTGGGAATAAAAAGCTGCCTAAGGTGAAAGTTAGAAATTGTAGACTTT340.13967627968120944No Hit
TATTTTGGGACCATGCTTTGGATGCTCTAGCATTTAGATATTGAGAAAATGA340.13967627968120944No Hit
TACGCAGGGACGACAGGTTTGGTTTTTAAATTTTCCAACCAAGTGGAAAGGC330.13556815380823267No Hit
AACCAAGGGCAGATGGTGAAAGAAAACCTGAGGAAAGTGGGACAACCAGTCA330.13556815380823267No Hit
CACATTGGGATAGGAGATGATCTTCAGAGAAAACACTTGAAATTGCTACTTT330.13556815380823267No Hit
TGTAATGGGTCTACAGATTATTTGAGAGTCAGTGATAGAGTAACTAGATATA330.13556815380823267No Hit
CGCATTGGGATCTCAGCTACTTTTTTCAATTTTATTATTTCCATTTGGTTCT320.13146002793525594No Hit
TGACATGGGACCTTTACCCTTCTCTTGAACACATCAAAGGATGTCATTGAGT320.13146002793525594No Hit
GACTATGGGACCCACATGCAGACACACACACACACACACACACACACACACA320.13146002793525594No Hit
TCACTTGGGGGTTGAATTTTAGGGGAACTTCACAAGGAATGGAATTCACCTT320.13146002793525594No Hit
GATCCCGGGAAATGATATATAACTATCTCAGAGCCTCAATTCCCACATTAAA320.13146002793525594No Hit
CTTGACGGGGTGAATGGTGTCTTCACTTATTATGTATGTTTATCTGTATGTA320.13146002793525594No Hit
GATTAAGGGGGGTTTTCTTATTTGTAAAAATAGATACTACTATCTAACTCCA320.13146002793525594No Hit
ATATGTGGGCATCAGAGAATAAAAAAAGTATTATAAAAATGAGTTAAAATCA320.13146002793525594No Hit
GGCGCTGGGCTTGTCTGAAGCTGATGGAAGATGGAACGGTTCAGTCCTCAGT320.13146002793525594No Hit
CAAAGGGGGTTTAATGGATTGGCATACAGCCTAATGGAGATTAAATACAGAT320.13146002793525594No Hit
AGACATGGGAGATCTTTTAAGACTTACACCCCAGAGATTTAAGAAGAAAACC310.1273519020622792No Hit
GGCAACGGGATCTAAGGAAGGGCTATGAAACTGGGCCCATCTGTATACAGGC310.1273519020622792No Hit
AACAATGGGAAGACCTACTGCTCAGAAAAAAGATTTCTTTCAAAATATTATT310.1273519020622792No Hit
AAAAATGGGGGGTAAGTAAGGAAGTCTTATAAATCTATTTTAGCCACCTAAC310.1273519020622792No Hit
ATTTTAGGGGTTTTCGGTAAGTGTGTCTTACAGAAAGTTGTGGTATTAAAGT310.1273519020622792No Hit
TTTTCGGGGGGAACAGATGACCTGAGATCAGAAAAGAGAGGTGCTCATTATT310.1273519020622792No Hit
TTTTTGGGGGATCTGAGTAGGGGAATACCACATAACCTTCCTAACTGTCCAG310.1273519020622792No Hit
TAGCTAGGGATTCTTAATCGCATATAAAAAGAAATCAGAGATTAATTTTATT310.1273519020622792No Hit
TTGGCTGGGATTTTAATAAATTTAAAATTAACTATGAAATGTCATATGAATG310.1273519020622792No Hit
TTACATGGGGATTCTTGATTGGCAGGAATTTTTTGTTTCTTTCTGTACTTAG310.1273519020622792No Hit
TTACAGGGGAATACTGATATTTTCCCCACCTCTCAATTGTTTTTAATTTTTA310.1273519020622792No Hit
CTTTGGGCAGCAAATTGGTCCCAGAATGTGCTTCGTATATTTTCCTCTTGAC310.1273519020622792No Hit
TTAGGTGGGATCCGTTTCTTGGCATCGGGCATGACAGCCTATCCAGCTTGGA300.12324377618930245No Hit
AATACAGGGAGAGTCTGCGCTGTGACCTTCTGTTGGGCGCGTGTCTGTTGGT300.12324377618930245No Hit
AGTAGCGGGATGCCTGGCTAATTTTCTTTCTATTTTTTGTAGAGACAGGGTC300.12324377618930245No Hit
ACAATTGGGACAGTTACCCTAGTGTGCAAAGAACGTCAGGACTTATACATAA300.12324377618930245No Hit
TCCTAGGGGGTTTATTTTAAATGTATGCCTTCCAAATGTTAAAATCTATATC290.1191356503163257No Hit
TGATAGGGGATGGCATTCTTTTAAAAATAGCAAAAGGTTAGGGAAAAATCCA290.1191356503163257No Hit
TTGTCGGGACTGCGGACCTCCAAAATTTTACAAAGGACTGCACAGTGAGTAT290.1191356503163257No Hit
AAGCTGGGGCAGTGTTATAAATTCAACATAGAGGCTGACAACCTAAATTCAT290.1191356503163257No Hit
TGCGAGGGATTGATACCATGGGGAATCATAGAAATAAAGTTGAATATCATTG290.1191356503163257No Hit
GATTAGGGGATGACAGAAATGACCTATCACTACTTATTATTTCTGAAGCCTA290.1191356503163257No Hit
CAGCTTGGGATTCTTCCTCCCTTAAATACCTGCGCTTGTACTCTCTAAGATA290.1191356503163257No Hit
GAATATGGGACAAAGGAGATGTGTAAACTGATAGATTTTGAGCCTAATAAGT290.1191356503163257No Hit
GATCAGGGGTAATATGTGTGATATGGGCTACTTCAGGACTCTCTGTTTTCTC290.1191356503163257No Hit
TTCATAGGGACAGGAAAACAAGGTGAAATGAGAGAAATGCAACAGCTTTCAG290.1191356503163257No Hit
AGGTTTGGGAGTGAGTGTTGATGAGGATGTGGAGAAAAGGGAACCCTGTGTG290.1191356503163257No Hit
TGAACAGGGAACTCTACAGACCAAACCATTTGTATCTGGCATCACTTACTAA290.1191356503163257No Hit
TTACGTGGGGAGTCTTGGGCACATTATTTAACCTCTCTGAGCTTTATTTTCC290.1191356503163257No Hit
GAATGAGGGAGATGAAATCGAGTGGCTATTGATCCGTGATGTTTCTAGATTT280.11502752444334893No Hit
TAACATGGGAACCCAGACAGTACTGAGTGACTAACTGGTGGGCGATATATAC280.11502752444334893No Hit
TTCATGGGGTTATGTTTATGAACAAACAAGGTGAAGGGTTCAGTATAAGTTG280.11502752444334893No Hit
ACACATGGGGAGCATTTATTCAACAAATATTTACTGAGAGTCTACCGTATGA280.11502752444334893No Hit
AACTAAGGGATTCTATTATAATTACAAGATGATTTTGTAAGCTTCACGGTAA280.11502752444334893No Hit
GACTTGGGACACATTTAATTATACAGCTTCTCTTTCTTAACCACAGGCAGAT280.11502752444334893No Hit
GTGCAGAGGGGAATATCAGAAGGCTATTACCACTGTTTGACCACAAAGGTAA280.11502752444334893No Hit
TCTTATTGGGAATCTGTGGCAGATTACTCTTTAAAACAATCAAACTTTTGTA280.11502752444334893No Hit
TTGAATGGGAAATGGGAATGGGAAGTACCATGTGGTGTTCAAGACACAACAT280.11502752444334893No Hit
TATAATGGGCTGGCAAAGAAGTTGCTGTTGGGGACTCGGAAGGCCGTATTTG280.11502752444334893No Hit
TACAGGAGGGGCAAATCAAAACCACAATGAGAGACCACACCCATGAGGATGG280.11502752444334893No Hit
TTTTGAGGGATCCATATAGAAACTTAAGCTCTTGACCTGAATTTTTCTGGCT280.11502752444334893No Hit
TGCTGGGATTGTATTCTACATATATAAATATGTATTTTTATTTTTTTACTTG280.11502752444334893No Hit
TGTGTGGGGGTTACTCTTAAAGATTGAAATTAAAAATGGTAAGGTGATTTGA280.11502752444334893No Hit
TACCGCGGGACAGCTGAGAGTGAAGCAAAAGTAAAAACCAAAGTTCGCTTTG270.11091939857037221No Hit
AGGTTAGGGGATAACATTGTGGAAGGAATAATTCGGTCTCCAGATTTAGTGA270.11091939857037221No Hit
CAAAATAGCTGTCTGTGTTGCAATCATTGCAAAATCTAAATTCTTTTGTTAC270.11091939857037221No Hit
AAGTTCGGGGGCAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAG270.11091939857037221No Hit
CGAGGGACTGGCTTTCTGTGTGTGTTTTTTTTCTGTTTTTAATCACCACGAG270.11091939857037221No Hit
TCTTATGGGAGACTCCAAGTTTTGGGACTCAGACTGAGTCACTACTGGCATC270.11091939857037221No Hit
TTTCGCTGGGTTTATAGGCACGAACCACCACTCCCGGCCACTTTTAACTTCC270.11091939857037221No Hit
ATTGTAGGGATGCTGTAAAGCTTGTAGCCTGAGAATACTATAGATTTCACCA270.11091939857037221No Hit
ATAGCGGGATGCTTCAAAAATTTTGTAAATTGTTTATTTTAAACTTATCTGT270.11091939857037221No Hit
TATTTAGGGATCAACCGCCAGATCAACCTGGAGCTCTACGCCTCCTACGTTT260.10681127269739546No Hit
GATATTGGGCTGAGGATTGAAACTTGATCTTGTCCAGGAAAGCTTTGTACCA260.10681127269739546No Hit
AACAGGGCCCATATATGCTTGAAACTACTGATAGTACTGAACCCTATATACA260.10681127269739546No Hit
TGATACGGGTGGGATAAGAGTCCCACAGTCTAGGGAGATCTGAGGGCATCCG260.10681127269739546No Hit
AAGCATGGGATGAGACAATTGAGTTTTACTCTTTGGAAGACTAAACCAATCA260.10681127269739546No Hit
AATAGGGGTAAGTGGGTGCCTCTCAGCACATTTCTATGAATATATTATGTAG260.10681127269739546No Hit
GGCATAGGGAGCCAAATTCCTCTTGAGAAGCAAGAGATGTTACAGAATGTTA260.10681127269739546No Hit
AACTTAGGGACGCTCTTCCGATCTTTTACTGGGATATTCCTTTGGGAATATA260.10681127269739546No Hit
TATGAGGGGGAATACCTGCCCTCCACAAACAGACTAAATATTAAGCATGTTT260.10681127269739546No Hit
AGTCCAGGGAGGCACATAGTCATCATGTTGTCTTAAGTCAAGACAAAGGAAA250.1027031468244187No Hit
CAATACGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTG250.1027031468244187No Hit
TAGCCGGGGATCTTCTTAGACTTAAAATTGAACTTGAGATAAATAAGGAAGA250.1027031468244187No Hit
TGCTCACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC250.1027031468244187No Hit
ACATACGGGGGGGCTAAGGAACAGATGAGTAATAAGAGGCTCTTGGATTTTT250.1027031468244187No Hit
TAATTCTGGGAGCATTAGCATTTCTTCTTATACCATGTTGAAATACTTTTAA250.1027031468244187No Hit
GACAGGGAACCAAGTTTATTCAGTAAACAACTTGTACTTGTTTCATCTGGTT250.1027031468244187No Hit
ACCCGCGGGAGCATTTGAAATCATAGATCAGTATTTCTCTGGTGATGATATT250.1027031468244187No Hit
AGCTTTGGGATTGATTCATTCAATTGAAGAAATCTGAATAAATTAGTAAATA250.1027031468244187No Hit
TTGCCTGGGATGAGGATTAGGCGAACACTTTGTAATACTTTGCCCAAGAAAA250.1027031468244187No Hit
CAGCGCCCTGCCGCCGCCGCCGCGGCCCAGCGAGCGGCCCTGATGCAGGCCA250.1027031468244187No Hit
GTCGTTCGGGGATAAACATGCTGTTAATGGCATGGACTCTGGTGTCAGTCTG250.1027031468244187No Hit
TTAAGGCGGGGGAGATGGAGGAAAGAATGTTGTGAGCTTTTTCATCAATTAT250.1027031468244187No Hit
AATTGGGGCTTCACAGGGACTGTGTCATCATCAGCTTAGGAAATAAAGACCT250.1027031468244187No Hit
AGCTCTGGGAATAACAACCACAAGAAAAGCCATACATCTTAATGAACATACG250.1027031468244187No Hit
CTAGAGGCGGGAAACAAAGCCCAATTCCTCATCAGATTTCTGTAAATAGCAT250.1027031468244187No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)