FastQCFastQC Report
Sat 31 Dec 2022
SRR5845293.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845293.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24430
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC800.3274662300450266No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT590.2415063446582071Illumina PCR Primer Index 5 (95% over 21bp)
GCACAGGGGGGGCGAGCGACCGAGCGCCGCGACGCGGAAGTGAGGTGCGTGC460.1882930822758903No Hit
AAGCTGGGGCAGTGTTATAAATTCAACATAGAGGCTGACAACCTAAATTCAT450.18419975440032746No Hit
TACGCAGGGACGACAGGTTTGGTTTTTAAATTTTCCAACCAAGTGGAAAGGC440.18010642652476463No Hit
GTGACAGGGGGTAAGGTTAGTAGTGATGCCCTCTATTTATTACCAGATTTTA430.1760130986492018No Hit
TTTTCAGGGATTTAGTAGGAATGTAAAGATACAAGTAGACAGAAAGTCTTCT420.17191977077363896No Hit
ATATCGAGGGAAACAAAATAAAGGCAATAGTGTATGTAAAAATGAAAGCACT420.17191977077363896No Hit
ACACATGGGGAGCATTTATTCAACAAATATTTACTGAGAGTCTACCGTATGA400.1637331150225133No Hit
GACTTTGGGAATAAAAAGCTGCCTAAGGTGAAAGTTAGAAATTGTAGACTTT400.1637331150225133No Hit
TCTTATGGGAGACTCCAAGTTTTGGGACTCAGACTGAGTCACTACTGGCATC400.1637331150225133No Hit
TTACAGGGGAATACTGATATTTTCCCCACCTCTCAATTGTTTTTAATTTTTA400.1637331150225133No Hit
GAACATGGGATTCCATGCTTTTCTGTGTTTATAAATCTAGGGTTACATGATG390.15963978714695046No Hit
TCAATTGGGGATCTGCAAGAGGGATGTCATGGGCTATGTTACACAATTTTTG390.15963978714695046No Hit
AATTCAGGGTAAGCACAGTATTTATTGCATCAAATATGTACCACAAGAAATG390.15963978714695046No Hit
ACGTAGGGGATATTTGATCCAACTTTTATAGCAAAGCAAGATCATGATGAAT380.15554645927138763No Hit
GCTCTTGGGAAGGATAGAGTGATGACATTTATTTTCATTCACTTTGAATCTG380.15554645927138763No Hit
CTAGTAGGGCAGCCTCCACCTGGAAGAGAGCTAGGGCCGGGCAGGCCGGGCA370.1514531313958248No Hit
TCTACAGGGGTATTTACATATATTTTATATTAAGTTTCACATGAAATTATTC370.1514531313958248No Hit
GATTAAGGGGGGTTTTCTTATTTGTAAAAATAGATACTACTATCTAACTCCA370.1514531313958248No Hit
AACTAAGGGGACTTGAAATGTTAATGGAGAGATATTCACAACTCCAGTGTAA370.1514531313958248No Hit
AGTTTCGGGGATTATATTTTCATTTGCTTCTTCCAGGAATTTTAGGTATATA370.1514531313958248No Hit
AGGTTTGGGAGTGAGTGTTGATGAGGATGTGGAGAAAAGGGAACCCTGTGTG370.1514531313958248No Hit
AAAAATGGGGGGTAAGTAAGGAAGTCTTATAAATCTATTTTAGCCACCTAAC360.14735980352026196No Hit
TTGAATGGGAAATGGGAATGGGAAGTACCATGTGGTGTTCAAGACACAACAT360.14735980352026196No Hit
AACACGGGACCGATGATTCTCCGTATAGCTGGTCTTTTCCACCTTATCATCC360.14735980352026196No Hit
AATGTTGGGACAGAAACCTAGCCATTCAAAATCTGTTTAAAGGACAAAGTTT360.14735980352026196No Hit
AGACATGGGAGATCTTTTAAGACTTACACCCCAGAGATTTAAGAAGAAAACC350.14326647564469913No Hit
TTAAGGCGGGGGAGATGGAGGAAAGAATGTTGTGAGCTTTTTCATCAATTAT350.14326647564469913No Hit
TGTAATGGGTCTACAGATTATTTGAGAGTCAGTGATAGAGTAACTAGATATA350.14326647564469913No Hit
GCTCTTGGGGAGCCACCACACCCAGCCTCAAAATATGGCCCTTTTTAAAGAG340.1391731477691363No Hit
GCTGTTGGGTAAGATGTATATTAAAGTAAAGCTACATTTTTTTACTTCATTA340.1391731477691363No Hit
AAAGTAGGGGATCATCTGGCTGAGCAACTGAATTACAAAAGAGAATTACTTC340.1391731477691363No Hit
TAGCTAGGGATTCTTAATCGCATATAAAAAGAAATCAGAGATTAATTTTATT340.1391731477691363No Hit
TTACGTGGGGAAGATGGAGGAGGAGATGGGATCAGAAACATAGCTGGATAAA340.1391731477691363No Hit
AATTGGGGCTTCACAGGGACTGTGTCATCATCAGCTTAGGAAATAAAGACCT340.1391731477691363No Hit
CTCAAAAGAAGACATTTATGCAGCCAACAGACACATGAAAAAATGCTCATCA330.13507981989357348No Hit
CGTCGAGGGAAGTCAAGTGCCAATTTCTAGGTCTTTTGTTCAATTTCTTTAT330.13507981989357348No Hit
ATATGTGGGCATCAGAGAATAAAAAAAGTATTATAAAAATGAGTTAAAATCA330.13507981989357348No Hit
AAGCATGGGATGAGACAATTGAGTTTTACTCTTTGGAAGACTAAACCAATCA330.13507981989357348No Hit
AGACATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC330.13507981989357348No Hit
TTTTGAGGGATCCATATAGAAACTTAAGCTCTTGACCTGAATTTTTCTGGCT330.13507981989357348No Hit
GTAACCGGGGGGACACACATACTTTTAGAAGTTAATAGAAGTCTTGAAGTCT320.13098649201801063No Hit
TGAACTGGGATTGCCACAGTGGTTTTATTTTTATTGTGTGATGTAACCATAT320.13098649201801063No Hit
CTTGACGGGGTGAATGGTGTCTTCACTTATTATGTATGTTTATCTGTATGTA320.13098649201801063No Hit
GCGCAGGGATTGAAATCTGCAGTGAGATATAACTTTCTAGGAACAACAAAAT320.13098649201801063No Hit
TTCATGGGGTTATGTTTATGAACAAACAAGGTGAAGGGTTCAGTATAAGTTG320.13098649201801063No Hit
AATACAGGGAGAGTCTGCGCTGTGACCTTCTGTTGGGCGCGTGTCTGTTGGT320.13098649201801063No Hit
GACAGTGGTGTCCCGGTGGTAATTGAAGGAAAACAGTTGTAATAGTAATAGC320.13098649201801063No Hit
AAGGCTGGGCATACATTGAGATAATATATTGCAGCTAAAGATAGGATAGGGA320.13098649201801063No Hit
CACTGGGGATTTCTGCATGGTCAAGTCCTCCTCATTCTTCATATTTCAGCAC320.13098649201801063No Hit
GGGCCGGGGATCTCAAGAAAAGAAGAAGTTCACAGATGAATTTCTAAAATCT320.13098649201801063No Hit
CTCAGTGGGATTTCAGGATATAAAGCAAATTATTTTCTAAGACATTTACAGT320.13098649201801063No Hit
AGTGCAGGGCACAAAAGGGCTCTGTGGTTTGCCTCCATGTGCACTGGCCCCT310.1268931641424478No Hit
TATAATGGGGACACACAACTTATTCTTCTGATGTTCAATATATGCTGATATT310.1268931641424478No Hit
ATTTTAGGGGTTTTCGGTAAGTGTGTCTTACAGAAAGTTGTGGTATTAAAGT310.1268931641424478No Hit
AGCACAGGGTCACTAGACCTTTGCTGTACTGGATTTCACAGGTGAAAAGATT310.1268931641424478No Hit
CAAAATAGCTGTCTGTGTTGCAATCATTGCAAAATCTAAATTCTTTTGTTAC310.1268931641424478No Hit
GTCGTTCGGGGATAAACATGCTGTTAATGGCATGGACTCTGGTGTCAGTCTG310.1268931641424478No Hit
TATTTTGGGACCATGCTTTGGATGCTCTAGCATTTAGATATTGAGAAAATGA310.1268931641424478No Hit
ATTGTAGGGATGCTGTAAAGCTTGTAGCCTGAGAATACTATAGATTTCACCA310.1268931641424478No Hit
TAGCCAGGAGAGATCATGATTAATGCAGAAGGTATAAAGAAGTTGTCCATTG310.1268931641424478No Hit
TAAACAGGGTAGTTTTTTGTTTGTTTGTTTTCCATTAGTTATTTTTATCTAC310.1268931641424478No Hit
GAATGAGGGAGATGAAATCGAGTGGCTATTGATCCGTGATGTTTCTAGATTT300.12279983626688497No Hit
AAACAGGGGAACCTTCCTTAAGTACAATTTAACTTCAAGAAAACAAAATTTG300.12279983626688497No Hit
GTATGAGGGGTAGTCTTTCCAGAAATATTTGTGGGATGACTGAACAGCCATT300.12279983626688497No Hit
TAACAGGGGATCTGCCTTTTGTTGTACCCTAGGGCATCCTTTCTACCAGAGT300.12279983626688497No Hit
TTAAAAGGGAATCCAGCTTTGCACTATATACTTATAGGAAAGCAAAACCACA300.12279983626688497No Hit
TAACCAGGGGCATTTGCCTGTTAATAGACTTATTAACATGTATGCTAGTAGT300.12279983626688497No Hit
TTAGGTGGGGGAAAGTGAGGCTTACCAATCACAACTTCTAGTAAATTAAAAG300.12279983626688497No Hit
CGTAATGGGGGGATTTTTCTGATTGTTTTGATTAATATATATGTTTCCAATG300.12279983626688497No Hit
ATCAATGGGGGAGAGGTTGGCTTTTCCTGAATTATTCATATATAACTAAAAT300.12279983626688497No Hit
CACATTGGGATAGGAGATGATCTTCAGAGAAAACACTTGAAATTGCTACTTT300.12279983626688497No Hit
AACTTAGGGACGCTCTTCCGATCTTTTACTGGGATATTCCTTTGGGAATATA300.12279983626688497No Hit
ACGCTTGGGGGATACCAAGTGTTTATATGGGTAGCTTGACTTGAGAGATTGA290.11870650839132214No Hit
AGATGCGGGGATCATGCCACTGCACTCTAGCCTGGTTGACAGAATAAGACCT290.11870650839132214No Hit
GAGTCAGGGGCTAGTAGCGGGCAAGATGAAATAATAGATAAAAGCTATACTC290.11870650839132214No Hit
TAACTAGGGCGATCTAACAAAGGGCGATCTAAAGCTGGGAGTGGTGTCTATC290.11870650839132214No Hit
AAGTTCGGGGGCAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAG290.11870650839132214No Hit
GGCGCTGGGCTTGTCTGAAGCTGATGGAAGATGGAACGGTTCAGTCCTCAGT290.11870650839132214No Hit
TCTAGTGGGATGGTGGTGTGCATTTGGAACTGGGCATGCAAACTTGAATTTG290.11870650839132214No Hit
AATATGGGGGGTTTGCATTTTAACAATTAGGATGGTTTATTGATGTGAATTT290.11870650839132214No Hit
TTATTGGGGATTTTGACAAAATTCAATACCCTTTCATGACAAAAATCACTGA290.11870650839132214No Hit
GGAAGTGGGAATCAAAATGGCAAATTATAAGATATTTTAAATTGGATAATAG290.11870650839132214No Hit
CTTGCTGGGACCTTTGCAATTGCACCACTAATAAAAACATAGGAATCAAACA280.1146131805157593No Hit
AATTTAGGGTGGGGAGAAAAATCTTCAGTGTTTTACCTTGCTAACATTTTAC280.1146131805157593No Hit
ACCCGCGGGAGCATTTGAAATCATAGATCAGTATTTCTCTGGTGATGATATT280.1146131805157593No Hit
AACATGGGGAGAGTGTGGTCAGGCGGCTCGGACTGAGCAGGACTTTCCTTAT280.1146131805157593No Hit
GTAATTGGGATGCCTTTTTGAAGAGGAATAAGGATAGGGAAGGTAGTATTGT280.1146131805157593No Hit
TGTTATGGGGGCAGAGGCGCTTCTCACTTCCCAGACAGTGGGGCGGCCGGGC280.1146131805157593No Hit
TTACGTGGGGAGTCTTGGGCACATTATTTAACCTCTCTGAGCTTTATTTTCC280.1146131805157593No Hit
GCATTGGGGGAAGCAGGGGGGCAATGCAAGTGCAACTAGCCAGAAAAACCAC270.11051985264019647No Hit
GACTATGGGACCCACATGCAGACACACACACACACACACACACACACACACA270.11051985264019647No Hit
AACAATGGGAAGACCTACTGCTCAGAAAAAAGATTTCTTTCAAAATATTATT270.11051985264019647No Hit
CATGAAGGGAATGTCAAGTGAAAATATTGACATATTATAATGAAGAATCCAG270.11051985264019647No Hit
TCATCGGGGGACACCACTGCAGTGTGAGCAAGGTGAATCCGTGTGCCATGCA270.11051985264019647No Hit
TGCTCACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC270.11051985264019647No Hit
TTCATAGGGACAGGAAAACAAGGTGAAATGAGAGAAATGCAACAGCTTTCAG270.11051985264019647No Hit
ATGCTTGGGGATCTTAGATTTTTGACTTATGAGGCCAGTGGTTTAAACAATC270.11051985264019647No Hit
ATTTCAGGGAGTAGAAAAATATGTGTAAAATAGGGAAGAAAGTTAGTATTGG270.11051985264019647No Hit
TATGCAGGGACGGTTAAATTCATGCACATTTGTTCTTGTAGTTTCTAAAAAT270.11051985264019647No Hit
TATAACGGGGAAGTTTTAATACACTCCTCACAGTATCTTATGGAAGTAACAC270.11051985264019647No Hit
CTTAACGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC270.11051985264019647No Hit
TAGCGTGGGCTCCCTCCTTAAATAAAGAGAGGGGTTAAACATGCCCTCTAAA260.10642652476463364No Hit
TCACTTGGGGGTTGAATTTTAGGGGAACTTCACAAGGAATGGAATTCACCTT260.10642652476463364No Hit
AAATGCGGGTGCGCGAGGGATGAATAATATGAAGGAGAGGTAGATTTCTGAG260.10642652476463364No Hit
CGCTCAGGGAAGGACCAGTTTGAATTTGCCCTGACAGCCGTGGCGGAGGAAG260.10642652476463364No Hit
AGTTGGGGAGCAAACATGATTAAGCTGAAGAAAGAATCAGGGAACTTGAAGA260.10642652476463364No Hit
AGTAGCGGGATGCCTGGCTAATTTTCTTTCTATTTTTTGTAGAGACAGGGTC260.10642652476463364No Hit
TGATACGGGTGGGATAAGAGTCCCACAGTCTAGGGAGATCTGAGGGCATCCG260.10642652476463364No Hit
AGCTTTGGGATTGATTCATTCAATTGAAGAAATCTGAATAAATTAGTAAATA260.10642652476463364No Hit
TTGGCTGGGATTTTAATAAATTTAAAATTAACTATGAAATGTCATATGAATG260.10642652476463364No Hit
CACAATGGGGCGGGCATTGGTGATATCCTGGAAGAAATTATCCGACAGATGA260.10642652476463364No Hit
TTACATGGGGATTCTTGATTGGCAGGAATTTTTTGTTTCTTTCTGTACTTAG260.10642652476463364No Hit
TGTGTGGGGGTTACTCTTAAAGATTGAAATTAAAAATGGTAAGGTGATTTGA260.10642652476463364No Hit
GGCAACGGGATCTAAGGAAGGGCTATGAAACTGGGCCCATCTGTATACAGGC250.1023331968890708No Hit
CAACAGGGATAGAATGTAAGCAGAAATAATGTCATAAGCAATTTTATATGAA250.1023331968890708No Hit
AGCAAATGGGGGGCGTGAGCCACCATGCCAGCCAGTAACAAATATTTTGTTC250.1023331968890708No Hit
GGGACACCCGGGAAGGTTGCTTGTTTGAGCATACCTATATACATGTTTGTTA250.1023331968890708No Hit
TTGTCGGGACTGCGGACCTCCAAAATTTTACAAAGGACTGCACAGTGAGTAT250.1023331968890708No Hit
GACTTGGGACACATTTAATTATACAGCTTCTCTTTCTTAACCACAGGCAGAT250.1023331968890708No Hit
TTCCATGGGAGAGCTGAGTGTCACTATTGCAGTTGGAGCATCACTGCTGTTT250.1023331968890708No Hit
CTTAATGGGATCATTGTGGTTTTCATTTGCACTTCCCAATGATTAGTGATGT250.1023331968890708No Hit
TTTTCGGGGGGAACAGATGACCTGAGATCAGAAAAGAGAGGTGCTCATTATT250.1023331968890708No Hit
GATCAGGGGTAATATGTGTGATATGGGCTACTTCAGGACTCTCTGTTTTCTC250.1023331968890708No Hit
CACATAGGGAAGAAATCATCAACTTCATGGCTACCTATATAGACATAATTAC250.1023331968890708No Hit
TTTGCCGGGATTCATTGACAGGAAACAGTGGAACAACATCTACAAAATCCTT250.1023331968890708No Hit
GCATCAGGGGAACATTAGGCATGAGAAAACCAAATTTTAGGATATCACTACA250.1023331968890708No Hit
AGATGGGGGATGGGGACAGTTTGGAAAATTGACATTCATGTGACTTAGAGTC250.1023331968890708No Hit
AACCAAGGGCAGATGGTGAAAGAAAACCTGAGGAAAGTGGGACAACCAGTCA250.1023331968890708No Hit
CAGCGCCCTGCCGCCGCCGCCGCGGCCCAGCGAGCGGCCCTGATGCAGGCCA250.1023331968890708No Hit
CATACTGGGATTTTGAGGTTGTCTGTCTCGAAGAAAAAGAAAATAAAATGTA250.1023331968890708No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)