FastQCFastQC Report
Sat 31 Dec 2022
SRR5845291.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845291.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132994
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT4750.3571589695775749Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC4220.3173075477089192No Hit
TATTAAGGAGACACGTTTCAAAAGACACCAACGCACGGCCAACAAGCACATA1450.10902747492368076No Hit
GCTCGCGGGAGATCTTCTCATGTGATGCTAGAGATGGGACTGGAGAAGTCAC1400.10526790682286419No Hit
GCTGGAAAAAATATTGATCATATTAAGTAGAGACATTAAAAATATCTACCTG1340.10075642510188429No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)