FastQCFastQC Report
Sat 31 Dec 2022
SRR5845290.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845290.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134345
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT4770.3550560125051174Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC4530.33719155904574044No Hit
GCTCGCGGGAGATCTTCTCATGTGATGCTAGAGATGGGACTGGAGAAGTCAC1580.11760765194089845No Hit
GAAGTAGGGATATAGCCATGGTTTTCAAACTGCCGCTCTAAAGCTAGGGAAA1510.11239718634858013No Hit
GAACTTGGGGAACAGGCAACCTACAACATGGGAGAAAATTTTCGCAACCTAC1410.10495366407383974No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)