FastQCFastQC Report
Sat 31 Dec 2022
SRR5845289.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845289.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131167
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT4620.3522227389511081Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC3990.3041923654577752No Hit
GCTCGCGGGAGATCTTCTCATGTGATGCTAGAGATGGGACTGGAGAAGTCAC1510.11512041900782971No Hit
GTTTGAGGGGTCTTAGTTTTTAACAAAAAAATTAAAAAGTAAAAAAACTAAA1460.11130848460359695No Hit
CTTAAATCGGGGTGTAGCAGTTTAATCAGTGGATAACTGAGCCTGTTTCCTC1410.10749655019936417No Hit
TATTAAGGAGACACGTTTCAAAAGACACCAACGCACGGCCAACAAGCACATA1330.10139745515259173No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)