FastQCFastQC Report
Sat 31 Dec 2022
SRR5845288.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845288.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32669
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT2100.6428112277694451Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC1850.5662860816064158No Hit
CCTTCAGGGGATCCTGAACGGCGCCTCCCGGAACCCGGCCGGTTAGTGGCGG550.16835532155866417No Hit
AAACCTGGGAAGTTGATTAAATCTTCTGAAATATAAAAAATTAAGAAGCCAG500.15305029232605835No Hit
ATTTCGGGGATAAGAGCAAAATGTTGTTACATAACCACCATACCATTTCCCC500.15305029232605835No Hit
GAGACTGGGAGACTAAACTGTACTTTCAAGTTCCCTGGTTTATTTCTCTTAA480.14692828063301602No Hit
TGTTAAGGGGATGAGATGAAATGAAATAGTGAAATGAAATTGAAATAAAATC470.14386727478649483No Hit
TCTGGGGTACGCACATTTGTCTGTGATTTGTATTCATAGGCAAGCTGTGGAC470.14386727478649483No Hit
GGCCAAGGAACGAGACCAAGATGATCACATTGACTGGCTCCTTGAAAAGAAG470.14386727478649483No Hit
GATTCTGGGTTACTTCACCTCTGAGCCTCCGATGCTCTGTGAAATGGAAGGA460.14080626893997367No Hit
CATCTGGGGAGTTGCTGCACTTGGATTTTCCTTTAATACCAAGTATCATTTG460.14080626893997367No Hit
AATAATGGGGTGTTTGAGTCTTGTATTGCTTGAAGATTTTTAGTTTTAGGCA460.14080626893997367No Hit
TTCAGTGGGATTTCACTAGATACTTACAATAACAGGAAATTGTTAATTCTTT450.1377452630934525No Hit
TATGCAGGGGGTGCGCCCTGGGCGCGCTCGGGATTCTCGCCTGGCGCGGCTG450.1377452630934525No Hit
GTTTCTGGGACAGATATATGAAACTATTCGCACAATTTAATTTTGGAACACT450.1377452630934525No Hit
TCTTTGGGGATCTCAGAAACAACACTGTGTTTAGCTCGGCTTTCTCTGCTAA450.1377452630934525No Hit
TACTCGGGGCTTCCGCAGGGTGTCGCCGCTGTGCCGCTAGCGGTGCCCCGCC440.13468425724693134No Hit
TCTATAGGGACAAAAACATGTGTTAGGCCAGATTTGGACCACAGGCTGTAGA440.13468425724693134No Hit
AATAGGGGTATAATGGTAAGACGATAAAAAGTATTAACTTAAAATGTTTTGA430.13162325140041017No Hit
AATATTGGGGAAACCTGTTATTCCAACAGCAGCAAAGCGAATTGATCTTTAT430.13162325140041017No Hit
CTAGCTGGGACTACAGGTGTGCACCACCACACAGGGCTAGTTTTTGTATTTT430.13162325140041017No Hit
ATAGCTGGGCATGGCTAAGAATGCAGGTGTTGAAGTATCTTTGATAGCTGAG430.13162325140041017No Hit
GTAACCGGGGGCCGTAGTTTGCGTTTTCACCTGGTCGCCCGGCGGCCATGGC420.128562245553889No Hit
CTTATCGGGGTACTTGTCTAGAGCCTTATTGTGACAGAGTTTTTAAAAATGC420.128562245553889No Hit
CTTACAGGGATCAAGGAAATGCAAATCAAAACCACAGTGAGGTATCATCTCA410.12550123970736785No Hit
CGCAATGGGGTACTGATAAAAACAAACATGGTTGTCCTTTACTTTGACAAAG410.12550123970736785No Hit
CTAGTGGGGAGTTGCTTTAGGGTCCATTTTTTTATGTAATCACTTACTCAGT410.12550123970736785No Hit
CACAAAGGGCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCACAC410.12550123970736785No Hit
CTAGAGGGGATAAGCATAGATGTGGTTTGGATTTTATTTTTCCTCTTGTCAA410.12550123970736785No Hit
TTTACTGGGAGGTTGAAAAGGAGTGCAAAGAAAAAGAGAAGCCCTCCTATGA410.12550123970736785No Hit
GCTACGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC400.12244023386084667No Hit
GTAATCCCAGCTACTCAGAGGCTGAGGCAGGAGATTCACTTGAACCCAGGAG400.12244023386084667No Hit
ATTCTCGGGGAAATCTCTGTTTAAGATCTCCACTTACAGTTTCTTACGGAAT400.12244023386084667No Hit
TCATATGGGACTAGACTCTTCTCTTGGATGCTGAGCAAAGCAAATGCTGAAT400.12244023386084667No Hit
GACTCGGGAGGGTGGTTTAGGACACCCAAGAATGCAGAATGGAAGCTGCCAC400.12244023386084667No Hit
CGACAGGGGTATCTAGATAGGATCATCAGAGGAAATCAACTTCAAGAATTTG400.12244023386084667No Hit
CTATACGGGGACGCCGGGGAATGACAAGTGGTAGATGACCTACTTGTGCAGA400.12244023386084667No Hit
GAGTTGGGGGCTATTGTCAAATTGTCCTATCGGGTTTTCCAGGTTTTGCCTG400.12244023386084667No Hit
AGACTGGGCTTTTAACTTTTCCAGCCTATTATAATAACTGAGTTATATTCAC390.11937922801432552No Hit
GACTCTGGGGATGATCTAGAACATTAGTCATCAACATTACATGTTTCATGCT390.11937922801432552No Hit
TACTCAGGGGGTTATAAAAAGGAAAAGTTTCAAAAGGGTATATTTTTCTATA390.11937922801432552No Hit
CGAACTGGGCTCGTTGCCATCTTAGTTTTGATGGGTTTTGGCTAGCGTCTTT390.11937922801432552No Hit
GACCACGGGAAGTTCTAATACGTTAAGTATTAATGATTTTAACCCATGTAAA390.11937922801432552No Hit
CGCTTAGGGTAGAAGAAACCACAAATTATCTTGTGCCTGAAATCCATTAAGA380.11631822216780434No Hit
GGATTCGGGATCTTTACTAGTTTGTTATACTTCCTCTTTAAGATGTTTTGTC380.11631822216780434No Hit
AGTAACGGGCCAAGCCTGTTTGTCTTCATTGTGACACTGGAGGTTTTGGATG380.11631822216780434No Hit
AAGTTTGGGTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAA380.11631822216780434No Hit
TTGGCTGGGGAAGTGTGGCAGTTTACTCTCTCCACTGTTGGAGAAGCCACCC380.11631822216780434No Hit
GCGCGGGCATACCCAACCCCCTGGTCAACCTCAACCTAGGCCTCCTATTTAT380.11631822216780434No Hit
GTTATAGGGGAAAAAATTAAGCCCCAAATTACCAGAAGGAAGAAAATAATAG380.11631822216780434No Hit
ACACCTGGGATTAGTTTGAATTCTAGAGACTAGATCTTAAAGTAGTGGTTCT380.11631822216780434No Hit
GGTATAGGGACTGGTCAGCTCCTTCGGACAAGGAAGGGTACAGTGGCGTGGG380.11631822216780434No Hit
ATTTACGGGCCATCACAGAGCTGTAAGTATACTACCTTCACATTATTACTGA370.11325721632128317No Hit
AAAAGTGGGTGAACTAAAGTATTTTTCTTAAGCCTATTGAGTGATTTATTTT370.11325721632128317No Hit
GACACCGGGGCCAGGAAGGCGAGTCCTCGCTGCATTTCTCCTCTCTGAGCAA370.11325721632128317No Hit
AACACTGGGAAATGACTTTGCTTTTACAGCCAAATGAGGAGACAGTTCTTGG370.11325721632128317No Hit
GTAACGGGATATAAATGGCCAACATGTATATGAAAAAATAAAAACCACAATG370.11325721632128317No Hit
TGAAGGGATTCTCGTCACTGTCCTTTCTATGTCAGCATTCAGAGTTACTGGC370.11325721632128317No Hit
TCAGCAGGGATCGGCTTGGAAATCAACGTGGTTGTCCCCGCCAGGCCGGGGA370.11325721632128317No Hit
CTAAGCGGGTAATTCCATGTTGATGGGTGAAACTTGTTTTTCTGAAAAATGA360.110196210474762No Hit
GTCTTTGGGAACCTTTTCATCTTGCAAAACTGAAACTTTGTACCCATTAAAC360.110196210474762No Hit
CTTAGCGGGTGCAGGCAAAGAACAGAATTCTAAAAACTTGGCTTTAAATAGG360.110196210474762No Hit
TGTGACGGGTGTTCAGTAGGTTGAACTATGAACACTGTCATCAATGTTCAGT360.110196210474762No Hit
GCGCCGGGGTGGGATAACTATAATGGAAGATTGTCATTATTTACCTGGCCTG360.110196210474762No Hit
TCTAAGGGGATCTACGGCGGGATCACAAAGGGAAAATAACCTTTAAAAACAA360.110196210474762No Hit
GATACCGGGACTAATGAGGAAACTGGAAACTGACAGGTTACATAAATTGTCC360.110196210474762No Hit
CACGCAAGGGAATCTTCTGACTTAAAAAGATAAAATGAGTATTTGTGATTTG360.110196210474762No Hit
TTGGAAGGGTGGTCTCTTCCAACTTCATGTACCAGCGTAAAGATTAAAGTGG360.110196210474762No Hit
TTGGCGGGGGGAAATACGAAATTGCAAATCTAAGTGAAAAACCTTTGTGTAA360.110196210474762No Hit
CAGAAGGGATTAGGTACTTATATCATGTGCTCTGTCATATCCAGGAAACAGG360.110196210474762No Hit
ACAACAGGGAGAGCAGGCAGCTGGTGTTCCTCTTTATTGGCTCAGACGCTTT360.110196210474762No Hit
CTATTGGGTGGAAACAAAGTAAACTATAAACTTGTACAAATTGGTTTTAAAA360.110196210474762No Hit
GCCAGCGGGATTATTGAAGGTGTAACTTGATAAAACTGTACCTGCGTTGCAG360.110196210474762No Hit
CTCACCAGGGCCTTTACCAAGTACTGCAAGAAATGGCAGGATGAGGATGGCA360.110196210474762No Hit
ATCTCTGGGACTACATAATTTGGTGTCATGCTCTTTTAAGCAAATCTGAGTA360.110196210474762No Hit
ACAGTGGGGTAATTAGTTTTGAGCTTCTTAGTAACCAAAATGAAGAAGCAAA350.10713520462824085No Hit
TGCTTAGGGAGGGGCGGTGGCCAGAGGAGCGAGAGCGGCGGCCCAGGCCCCG350.10713520462824085No Hit
GCTGAGGGATTGAAAGACAGGAAACCTTAAAAGAAAAACAAAACTGATAATC350.10713520462824085No Hit
GCGTAGGGGGTGTTTTAAATGGAGTCAGGACTTATTGGTTTTACTTCTTGAA350.10713520462824085No Hit
CACACAGGGGAGCCCTCAGGGGAAAGGGTTTAAGAAAAACAACTCCTCTTTC350.10713520462824085No Hit
TTTAGTGGGGGGAGTAAAGTGTTTGGGTGAAAGAATAATTTTTACTTTTATG350.10713520462824085No Hit
ACAGCGGGAGTTAAAGGCCTGGCCTGCAGCCCATGTGAGACCCTCTGGCAAC350.10713520462824085No Hit
CTTGTGGCGGGGAAGAAGCAAAATTAAAAGCAAGATATCCTCATCTGGGACA350.10713520462824085No Hit
TACAGGGAAGAACAACTGGATATACAAACAAAGAAATGTTAAAATATAAAAA350.10713520462824085No Hit
CTCTTTGGGGAAAGTTTTCATAAGTGAAAGGACTCTTACTGGAATTTGAATT350.10713520462824085No Hit
ACTTTAGGGACCAAACTGTTTTGGAACCCTTGCTCATGAAGGAATTTGCATT350.10713520462824085No Hit
TTAAGGGGGGAGTGGCAGAGTCTTATCACAGAAGGCAGCCACCATTTCACCA350.10713520462824085No Hit
CTGTTCGGGAAGAGAGCTGGTAATTGAAGGGCTAATATCTTACAGAGAAGGG350.10713520462824085No Hit
TTTACAGGGTAAACAAGCCAACATATGAATATATGTTTTGTCTCGCTATACT350.10713520462824085No Hit
ATAAAAGGGATACTGGAAAGTTCCTGATTTATAAAAATATTCAAATAGTGTT350.10713520462824085No Hit
GTAGAGGGGATCTAGAGTTCTCTTTTTAAAAGCATATTCTTAATTACTAATT350.10713520462824085No Hit
GATCATGGGGTACTGCTGACCTTGGGTTTGTTTTTGTTTTGTTTTGTTTTTC350.10713520462824085No Hit
GGTTAGGGGTGCCTTACCAAGATCATGATGAGTACAGTTATCTCAGGATTCT350.10713520462824085No Hit
CAGTTAGGGATTGTCTACCTCCCTTTATTTCTGCCTGGAGCCAAGCGGGGAA340.10407419878171967No Hit
TTACCAGGGACTGAATGTGAATTTTGCTGTTTTTTTAATAGAAAATAAAATA340.10407419878171967No Hit
CGTCACGGGTTCCACTTCTCACAAGCCGAGAAGGTGTACATCATGTAGGCCC340.10407419878171967No Hit
GCCCCAGGCATAAAGAAGGAAAATTGGCCATCTTTCCCACCTCTAAATTCTG340.10407419878171967No Hit
TATTTTGGGCAGTTTCAGTCAGGGACATATTTTGTGCAATATTTCTGTGATT340.10407419878171967No Hit
CACAAGTGGGATGTTCATAATAACTTGTGTTCATTGTTCACAACAAAACAAA340.10407419878171967No Hit
TCCACTGGGGACACCCTAACATCACAATTAAAAGAACTAGAGACGCAAGAGC340.10407419878171967No Hit
GTGACGGGCGGAAAGGCCAAGACAAAGGCGGTTTCCCGCTCGCAGAGAGCCG340.10407419878171967No Hit
AATTGAGGGGACAGTCTCAAAAACAAACAAACAAACAAAAAAACTACTCTGT340.10407419878171967No Hit
TGTCAGGGGGTACAAGTCAGCAGTAAAGAACTAGAGATTTAAAAACCTTCTA340.10407419878171967No Hit
TTGCCAGGGTGTCATTTTTTGTGTTACAGAACTATAATGTGATTCAAGGTGT340.10407419878171967No Hit
TGTTTTGGGTGGAGCACGATTTAAAAAGAGATCAGTGTTTTCCATTCTCTTT330.10101319293519849No Hit
TGCCAGGGGGTTATTCTAGGAGGAGCATACTTGTACAAATATTTTGCACTTC330.10101319293519849No Hit
CTCGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA330.10101319293519849No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCTTATAC330.10101319293519849No Hit
GACCTAGGGCAGATATATCAACATATTAGCATTAATTTTGACTTGATAAAAA330.10101319293519849No Hit
CACATTGGGTGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCA330.10101319293519849No Hit
AACTGTGGGTTGACTTTGTGGGAAATAAAAAGGGCACTCCTTCAGGGCAGAT330.10101319293519849No Hit
TGTTACGGGAAGCTGAATTGGCTGTGTTCAAGGCATGCCCTTTAGAATTGAA330.10101319293519849No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)