FastQCFastQC Report
Sat 31 Dec 2022
SRR5845287.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845287.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33146
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT1850.5581367284136849Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC1640.4947806673505099No Hit
TACTCGGGGCTTCCGCAGGGTGTCGCCGCTGTGCCGCTAGCGGTGCCCCGCC590.17800036203463465No Hit
GTGCATGGGATACAAAATATGAAAACAATACTAGTCTTCAATATATCAAAAT590.17800036203463465No Hit
ACAGTGGGGTAATTAGTTTTGAGCTTCTTAGTAACCAAAATGAAGAAGCAAA560.16894949616846677No Hit
TTCAGTGGGATTTCACTAGATACTTACAATAACAGGAAATTGTTAATTCTTT540.16291558559102154No Hit
GCTACGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC530.15989863030229892No Hit
GTAACGGGATATAAATGGCCAACATGTATATGAAAAAATAAAAACCACAATG490.14783080914740843No Hit
AAAAGTGGGTGAACTAAAGTATTTTTCTTAAGCCTATTGAGTGATTTATTTT480.1448138538586858No Hit
CACAAAGGGCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCACAC480.1448138538586858No Hit
TCTGGGGTACGCACATTTGTCTGTGATTTGTATTCATAGGCAAGCTGTGGAC470.1417968985699632No Hit
TGTTTTGGGTGGAGCACGATTTAAAAAGAGATCAGTGTTTTCCATTCTCTTT460.13877994328124058No Hit
CTGCCGGGGGGCCTGGAAGTTTAGTTTATGATAAAAGACATATTTCACCTAA460.13877994328124058No Hit
GTAACCGGGGGCCGTAGTTTGCGTTTTCACCTGGTCGCCCGGCGGCCATGGC460.13877994328124058No Hit
ATTTCGGGGATAAGAGCAAAATGTTGTTACATAACCACCATACCATTTCCCC450.13576298799251796No Hit
GCCAGCGGGATTATTGAAGGTGTAACTTGATAAAACTGTACCTGCGTTGCAG450.13576298799251796No Hit
CTTACAGGGATCAAGGAAATGCAAATCAAAACCACAGTGAGGTATCATCTCA440.13274603270379534No Hit
CTTGGCGGGATCTTCATAAAACATCTTTATAGTCTTTCTAAATACCTTGAAT440.13274603270379534No Hit
CATCTGGGGAGTTGCTGCACTTGGATTTTCCTTTAATACCAAGTATCATTTG440.13274603270379534No Hit
CAGAAGGGATTAGGTACTTATATCATGTGCTCTGTCATATCCAGGAAACAGG440.13274603270379534No Hit
AGACATGGGATCCCACCTCACTTCCCTTAGTTCACAGCAAACAAGCCAAATC430.1297290774150727No Hit
TGTTGCGGGAGCTAACATGAGAGAGAAGAGGAAGGGTAGTAAATAGAATTGA420.1267121221263501No Hit
CATGCAGGGGACAGAAACCAGGCGATGACAAATAAATGCCACTAAACATATC420.1267121221263501No Hit
CAAACTGGGATTGAGACCTATTTCTCTAGGTTATCCTTTGCCAGTTGTTTTC410.12369516683762748No Hit
GCGCCGGGGTGGGATAACTATAATGGAAGATTGTCATTATTTACCTGGCCTG410.12369516683762748No Hit
AGAGCGCGGGTATCTGCATATGCCAAAAAATTTTAAGCAAATGAAAGCTACC410.12369516683762748No Hit
TATTTTGGGCAGTTTCAGTCAGGGACATATTTTGTGCAATATTTCTGTGATT410.12369516683762748No Hit
CTAAGTGGGAGCTTTGAATGATTTTTGCACAGCTATAAAGTATTTAGGATTC410.12369516683762748No Hit
CCTTCAGGGGATCCTGAACGGCGCCTCCCGGAACCCGGCCGGTTAGTGGCGG410.12369516683762748No Hit
AGAAGAGGGGACTCACAGACAGTGATGAGATGAGGGTGGTGAGATCATGCAG410.12369516683762748No Hit
AATCTTGGGAGAAGCAACATACTTACTTGTATCCTGGCTTTGGCTAGCTAAA410.12369516683762748No Hit
TAGTGGGAAATGAAATAATGAAATGAGATGAAATAAAATAATGAAATGATGA410.12369516683762748No Hit
GTCTTTGGGAACCTTTTCATCTTGCAAAACTGAAACTTTGTACCCATTAAAC400.12067821154890485No Hit
GCTTGTGGGTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAA400.12067821154890485No Hit
TAAGCCGGGATAAATCAAATACTGAACAGTAATAATCTTGAAGAGTTGTGAA400.12067821154890485No Hit
GATTTTGGGAGAGGGAGACCCTGTCTCAGGGAGAAAAAAAAGAGAAAGCACA400.12067821154890485No Hit
CCTGACTGGGGGAAAAACAGTGTTATATAGGAACTACCAATTTTAGTACAAA400.12067821154890485No Hit
GCTGCAGAGCAGTGCTGTGCCAGGCTAGCAGAGGCATTTACTGAGTTGTGTA400.12067821154890485No Hit
TCTATAGGGACAAAAACATGTGTTAGGCCAGATTTGGACCACAGGCTGTAGA400.12067821154890485No Hit
GTTAACGGGGGACATATATTTTAAGAGGAGAGTGTTCTAAAGATAAGTTTAG390.11766125626018221No Hit
AAACCTGGGAAGTTGATTAAATCTTCTGAAATATAAAAAATTAAGAAGCCAG390.11766125626018221No Hit
TATTATGGGTCAACTACTTTATATTATTTTCTGAAATATTTCCTTGGCTTTT390.11766125626018221No Hit
ATTAATGGGAGATGTGGACAAAAATATCAGCATCAGTGATGTGACACCCAGT390.11766125626018221No Hit
ATTCTCGGGGAAATCTCTGTTTAAGATCTCCACTTACAGTTTCTTACGGAAT390.11766125626018221No Hit
TTCACAGGGATTATCTCAATTAATCCTCACAATCAAATTCTGAGGTAGTTAC390.11766125626018221No Hit
ACAGTTGGGAACGCAAAGAACTTGGACAACTGGTAGTCAAAACCTTCCCCCG380.11464430097145961No Hit
TGCTGTGGGACCAATCGTGAAAACTATATTCACAGAATTGGCAGAGGGGGTC380.11464430097145961No Hit
AGTAACGGGCCAAGCCTGTTTGTCTTCATTGTGACACTGGAGGTTTTGGATG380.11464430097145961No Hit
TCTAAGGGGATCTACGGCGGGATCACAAAGGGAAAATAACCTTTAAAAACAA380.11464430097145961No Hit
CTATTCGGGACCTCAAAAAGAACACACTAAGTCCCCAGCAATGGTTCCCAAC380.11464430097145961No Hit
ATTTATGGGATTCAGATAGTGGGTGGAAATGTACATGTATGGTAAGCATTGC380.11464430097145961No Hit
AACAGTGGGACCAAGGCACCAAGATGATACCCTGTGACTTCCTCATCCCGGT380.11464430097145961No Hit
TTCAACGGGCGATCAGCCAAGGGATGACCCCAATACGCAAAACTAACCCCCT380.11464430097145961No Hit
GACCACGGGAAGTTCTAATACGTTAAGTATTAATGATTTTAACCCATGTAAA380.11464430097145961No Hit
CTAAGCGGGTAATTCCATGTTGATGGGTGAAACTTGTTTTTCTGAAAAATGA370.11162734568273698No Hit
GAACTCGGGTGGCTTTCAAGGGCACCAAGAAGAGATCCCAGTTAGATCTGGA370.11162734568273698No Hit
GAAGTAGGGGATGTGATCATCCTAAGCCTGCTTTCTTGGCTCAAGGACACCA370.11162734568273698No Hit
GTAATCGAGGGATTGCTTTAGATCTATGCTGTATCTGGTCTTGAGACTTTAA370.11162734568273698No Hit
GACTATGGGACTATTTCCTCATTTATAAAATAAGGAAAATTATTCTTACCTC370.11162734568273698No Hit
AAGTTTGGGTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAA370.11162734568273698No Hit
TCACGCCGGGACAAATATACGAGAACAGGGGTTTTAGCCTAGAGTGGCTGGA370.11162734568273698No Hit
TGCCCAGGGAGTATTAACTATAGTCTTCCTAGAGTGGTATGGAACACTAGAA370.11162734568273698No Hit
TTAAGGGGGGAGTGGCAGAGTCTTATCACAGAAGGCAGCCACCATTTCACCA370.11162734568273698No Hit
TTTACTGGGAGGTTGAAAAGGAGTGCAAAGAAAAAGAGAAGCCCTCCTATGA370.11162734568273698No Hit
GTATATGGGGGCAATTATTTTTAGATATGATATCAAAAGCGTAAGCAGCAAA370.11162734568273698No Hit
GTGCAGGGGTACAGAATGAATTGAGCAAAGATGGCCTAGCATTCGTATTTAG360.10861039039401436No Hit
GACACCGGGGCCAGGAAGGCGAGTCCTCGCTGCATTTCTCCTCTCTGAGCAA360.10861039039401436No Hit
CAGTTAGGGATTGTCTACCTCCCTTTATTTCTGCCTGGAGCCAAGCGGGGAA360.10861039039401436No Hit
TGTTAAGGGGATGAGATGAAATGAAATAGTGAAATGAAATTGAAATAAAATC360.10861039039401436No Hit
GATCATGGGATTTTATTAACTAGGTTAACATCAACTGTGGTTGTAAGAGTAA360.10861039039401436No Hit
CCTCAAGGGTACTGATTCGATCTGCTTTATATATGAGAACGTATTTTTAACT360.10861039039401436No Hit
AAGCAGGGAACTGGCAATATTTCAAACATTTTATTATTATTATTTCTTTATG360.10861039039401436No Hit
AACTGTGGGTTGACTTTGTGGGAAATAAAAAGGGCACTCCTTCAGGGCAGAT360.10861039039401436No Hit
GAGTTGGGGGCTATTGTCAAATTGTCCTATCGGGTTTTCCAGGTTTTGCCTG360.10861039039401436No Hit
AATAGGGGTATAATGGTAAGACGATAAAAAGTATTAACTTAAAATGTTTTGA350.10559343510529173No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCT350.10559343510529173No Hit
GCCCAGGGGAGGGCGCCCAACAGCCCAGCCTCTACCAGATCCGCACCAACGG350.10559343510529173No Hit
CTAGCTGGGACTACAGGTGTGCACCACCACACAGGGCTAGTTTTTGTATTTT350.10559343510529173No Hit
CTAGTGGGGAGTTGCTTTAGGGTCCATTTTTTTATGTAATCACTTACTCAGT350.10559343510529173No Hit
CTAGAGGGGATAAGCATAGATGTGGTTTGGATTTTATTTTTCCTCTTGTCAA350.10559343510529173No Hit
CTTGTGGCGGGGAAGAAGCAAAATTAAAAGCAAGATATCCTCATCTGGGACA350.10559343510529173No Hit
CTTATCGGGGTACTTGTCTAGAGCCTTATTGTGACAGAGTTTTTAAAAATGC350.10559343510529173No Hit
CAGTATGGGATCTCACCTTTGCATGTTGGGGGACAGATAACGTGCATTTTTA350.10559343510529173No Hit
TTCAGTGGGATTCAAAATCTCTTCAGTTTATATTTTTCAGATTCATATTATA350.10559343510529173No Hit
TTCATAGGCCAGACATGAATATTTGCACTGAGTTTGGAATTTTCTCCCTTTT350.10559343510529173No Hit
ATCTTAGGGCTGAACTCTTTGACAAATTGCACAGAGCTCTTTGGCATTAATC350.10559343510529173No Hit
TGCTCGGGGCACGGACTGCCTGCAGTTCTACGTGAACAGCACACTTAAGGAA350.10559343510529173No Hit
AGCGCAGGGGAACCCCCCTGACCTTTCCTGGTGGGAAACGGGGACAGTACGA340.10257647981656913No Hit
TGTATGGGGGGACACAGCCTGGGACAGGAAGCCTCTTGGGTTGGAGCAGGAG340.10257647981656913No Hit
GTAACTGGGACCTACAATGGCTATCTATTACCTTATTCTCTGGTTTGTTTAA340.10257647981656913No Hit
GTGATTGGGGCTGTCTTATTTTTGCTGTGTCTTTTGACACCTCTTAGCTTTA340.10257647981656913No Hit
GACTCTGGGGATGATCTAGAACATTAGTCATCAACATTACATGTTTCATGCT340.10257647981656913No Hit
TCTGCTGGGATCAGAAACACACAATAATCCTATGTGTCTACGCACCTGAGAA340.10257647981656913No Hit
TTGGAAGGGTGGTCTCTTCCAACTTCATGTACCAGCGTAAAGATTAAAGTGG340.10257647981656913No Hit
TACGCTGGGGTACTGCAATGGTGGCCTACCTTTGCCTGTAATATTAGGCATA340.10257647981656913No Hit
GACCTAGGGCAGATATATCAACATATTAGCATTAATTTTGACTTGATAAAAA340.10257647981656913No Hit
AGGTTAGGGTATGTGGTAGTAGATGGATCAAGAACAATGGAAGACCAAGATT340.10257647981656913No Hit
GTTTCTGGGACAGATATATGAAACTATTCGCACAATTTAATTTTGGAACACT340.10257647981656913No Hit
GTTATAGGGGAAAAAATTAAGCCCCAAATTACCAGAAGGAAGAAAATAATAG340.10257647981656913No Hit
ACAAATATTTTCTCCCATTCTGTAGGTTTTCTGTTTACTCTGTTGACATTCT340.10257647981656913No Hit
GATGAGGGAACATGGTTTGTCTTTCTCTGTCACTGTACTTTTAATCTGTCTT340.10257647981656913No Hit
CTCACCAGGGCCTTTACCAAGTACTGCAAGAAATGGCAGGATGAGGATGGCA340.10257647981656913No Hit
ATAAAAGGGATACTGGAAAGTTCCTGATTTATAAAAATATTCAAATAGTGTT340.10257647981656913No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)