FastQCFastQC Report
Sat 31 Dec 2022
SRR5845286.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845286.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32879
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT2140.650871376866693Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC1590.48359135010188875No Hit
GTAACCGGGGGCCGTAGTTTGCGTTTTCACCTGGTCGCCCGGCGGCCATGGC570.173362936828979No Hit
GTGCATGGGATACAAAATATGAAAACAATACTAGTCTTCAATATATCAAAAT530.16119711670062958No Hit
CTTACAGGGATCAAGGAAATGCAAATCAAAACCACAGTGAGGTATCATCTCA510.15511420663645487No Hit
CTTGGCGGGATCTTCATAAAACATCTTTATAGTCTTTCTAAATACCTTGAAT510.15511420663645487No Hit
GAGACTGGGAGACTAAACTGTACTTTCAAGTTCCCTGGTTTATTTCTCTTAA510.15511420663645487No Hit
TTTAGTGGGGGGAGTAAAGTGTTTGGGTGAAAGAATAATTTTTACTTTTATG500.15207275160436753No Hit
GTAATCGAGGGATTGCTTTAGATCTATGCTGTATCTGGTCTTGAGACTTTAA490.14903129657228018No Hit
CATCTGGGGAGTTGCTGCACTTGGATTTTCCTTTAATACCAAGTATCATTTG490.14903129657228018No Hit
CCTGACTGGGGGAAAAACAGTGTTATATAGGAACTACCAATTTTAGTACAAA470.14294838650810548No Hit
AAAAGTGGGTGAACTAAAGTATTTTTCTTAAGCCTATTGAGTGATTTATTTT460.13990693147601813No Hit
CGCAATGGGGTACTGATAAAAACAAACATGGTTGTCCTTTACTTTGACAAAG450.1368654764439308No Hit
CAAAGGGTGACTTGGTCAGAAGGCTGGCTGTGTTCTGGAGTGCCATTCCTGC450.1368654764439308No Hit
GACCACGGGAAGTTCTAATACGTTAAGTATTAATGATTTTAACCCATGTAAA450.1368654764439308No Hit
GCCAGCGGGATTATTGAAGGTGTAACTTGATAAAACTGTACCTGCGTTGCAG450.1368654764439308No Hit
CAAACTGGGATTGAGACCTATTTCTCTAGGTTATCCTTTGCCAGTTGTTTTC440.13382402141184344No Hit
AATATTGGGGAAACCTGTTATTCCAACAGCAGCAAAGCGAATTGATCTTTAT440.13382402141184344No Hit
CTAGTGGGGAGTTGCTTTAGGGTCCATTTTTTTATGTAATCACTTACTCAGT440.13382402141184344No Hit
GCTATAGGGTCTATGATGTTTGCACAATGACACAATCGCCTAACGATGTGTT440.13382402141184344No Hit
ATAGCAGGGAAGTGGAAGTAAGAATAATATTCATGAAATGTCTTCTTTGGGC440.13382402141184344No Hit
TACTCGGGGCTTCCGCAGGGTGTCGCCGCTGTGCCGCTAGCGGTGCCCCGCC430.13078256637975608No Hit
AAGTTTGGGTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAA430.13078256637975608No Hit
AGTAATGGGACCATGAAACCAGGTTGAATTTTGTCAAATGCTTTTTCTGCAT430.13078256637975608No Hit
CCTCAAGGGTACTGATTCGATCTGCTTTATATATGAGAACGTATTTTTAACT420.12774111134766872No Hit
GCGCGGGCATACCCAACCCCCTGGTCAACCTCAACCTAGGCCTCCTATTTAT420.12774111134766872No Hit
CCTTCAGGGGATCCTGAACGGCGCCTCCCGGAACCCGGCCGGTTAGTGGCGG420.12774111134766872No Hit
AATAATGGGGTGTTTGAGTCTTGTATTGCTTGAAGATTTTTAGTTTTAGGCA420.12774111134766872No Hit
TCACGCGGGCAATATTTTAGAACTTTTTATGTATGAGGATAATCCTTTTAAA420.12774111134766872No Hit
TCTGGGGTACGCACATTTGTCTGTGATTTGTATTCATAGGCAAGCTGTGGAC410.12469965631558136No Hit
CACGTTGGGATTCATGTATAACTATCAGTTGCATTGTGTTTTTCTGAAAATC410.12469965631558136No Hit
GTCTTCGGGAAGTGATTTAGATATCTATCTATCTATCTAGATTTCTGAACCA410.12469965631558136No Hit
TTTACTGGGAGGTTGAAAAGGAGTGCAAAGAAAAAGAGAAGCCCTCCTATGA410.12469965631558136No Hit
CAAGCTGGGACACTGGACCAGATAGTAGACTGGTCCCTTGATCAGAAGCATC410.12469965631558136No Hit
TGCCGCGGGGTAGATATATCTGTTAATACGTAAAATGTTGCTTTTTATCAGC400.12165820128349403No Hit
CTAGCTGGGACTACAGGTGTGCACCACCACACAGGGCTAGTTTTTGTATTTT400.12165820128349403No Hit
ATAGCTGGGCATGGCTAAGAATGCAGGTGTTGAAGTATCTTTGATAGCTGAG400.12165820128349403No Hit
TCAGACGGGACCCCTTCATGGAGGCTCGGGCAGCTATTCGGTATGTTCAAAC400.12165820128349403No Hit
TCATATGGGACTAGACTCTTCTCTTGGATGCTGAGCAAAGCAAATGCTGAAT400.12165820128349403No Hit
ATTTCGGGGATAAGAGCAAAATGTTGTTACATAACCACCATACCATTTCCCC400.12165820128349403No Hit
TCAGCAGGGATCGGCTTGGAAATCAACGTGGTTGTCCCCGCCAGGCCGGGGA400.12165820128349403No Hit
TTCATAGGCCAGACATGAATATTTGCACTGAGTTTGGAATTTTCTCCCTTTT400.12165820128349403No Hit
ATCTTAGGGCTGAACTCTTTGACAAATTGCACAGAGCTCTTTGGCATTAATC400.12165820128349403No Hit
TTTGCTGGGATTACAGGCGTGAGCCACCGCACCTGGCCTAGAATAGACATTT400.12165820128349403No Hit
GCATCAGGGACTACCAGAAATGATTCAAGGTTTATTTCCTTCTGCTTCAGTT400.12165820128349403No Hit
CTAAGCGGGTAATTCCATGTTGATGGGTGAAACTTGTTTTTCTGAAAAATGA390.11861674625140667No Hit
CAGTTAGGGATTGTCTACCTCCCTTTATTTCTGCCTGGAGCCAAGCGGGGAA390.11861674625140667No Hit
TAACTTGGGTGGTTTCTAACGTTGGTTTTGTGAAATGTTATGATCTGTTATC390.11861674625140667No Hit
TGTTGCGGGAGCTAACATGAGAGAGAAGAGGAAGGGTAGTAAATAGAATTGA390.11861674625140667No Hit
CTAAGTGGGAGCTTTGAATGATTTTTGCACAGCTATAAAGTATTTAGGATTC390.11861674625140667No Hit
AACTCAGGGGAAGCTAAAATTCATCCTTTTATGAGGTGTGGAAGTCAGTGAC390.11861674625140667No Hit
GCAAACGGGAGAAGTTAAAGACCAGCCTGCGCAACATAACAAGACCCTGTCT390.11861674625140667No Hit
GTATATGGGAGAGATTAATACAACTACTTAAAAAATATAGTCAATAGGTTAC380.11557529121931932No Hit
GGATTCGGGATCTTTACTAGTTTGTTATACTTCCTCTTTAAGATGTTTTGTC380.11557529121931932No Hit
CTGCCGGGGGGCCTGGAAGTTTAGTTTATGATAAAAGACATATTTCACCTAA380.11557529121931932No Hit
ATTTTGGGGAAAGGAAGAACTAAAAGAACTTCTGAAAAAAGCAGCCAGTGGT380.11557529121931932No Hit
AGAGCGCGGGTATCTGCATATGCCAAAAAATTTTAAGCAAATGAAAGCTACC380.11557529121931932No Hit
CACAAAGGGCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCACAC380.11557529121931932No Hit
AGTTAAGGGCGATCTGCACGCGGGACTCCATATACTTTTTTTTCCTGTTTTT380.11557529121931932No Hit
CTTATCGGGGTACTTGTCTAGAGCCTTATTGTGACAGAGTTTTTAAAAATGC380.11557529121931932No Hit
TCTAAAGGGGAGGCACTGGTGGAATAAAAAACCTGTATATTTTACTTTGTTG380.11557529121931932No Hit
TGCGAAGGGCTTTGATTATCCCCTATAACCAACACTCAACTACAAATAGGCT370.11253383618723196No Hit
TGCTGTGGGACCAATCGTGAAAACTATATTCACAGAATTGGCAGAGGGGGTC370.11253383618723196No Hit
CTTAGCGGGTGCAGGCAAAGAACAGAATTCTAAAAACTTGGCTTTAAATAGG370.11253383618723196No Hit
TGTGCAGGGGAAAAGCAAGACATTAGTCAAGTAATCACACAAATAAATGTAA370.11253383618723196No Hit
GCTACGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC370.11253383618723196No Hit
ATAAAAGGGATACTGGAAAGTTCCTGATTTATAAAAATATTCAAATAGTGTT370.11253383618723196No Hit
GTAGAGGGGATCTAGAGTTCTCTTTTTAAAAGCATATTCTTAATTACTAATT370.11253383618723196No Hit
TTAATGGGGTTACTCCTAACTTGTCAAATTGATCTCTTTGCAGGTAACCAAG370.11253383618723196No Hit
TCTATAGGGACAAAAACATGTGTTAGGCCAGATTTGGACCACAGGCTGTAGA370.11253383618723196No Hit
TTACAGCGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGC360.10949238115514463No Hit
ACAGTGGGGTAATTAGTTTTGAGCTTCTTAGTAACCAAAATGAAGAAGCAAA360.10949238115514463No Hit
CGCTTAGGGTAGAAGAAACCACAAATTATCTTGTGCCTGAAATCCATTAAGA360.10949238115514463No Hit
GCTTCAGGGGGGGATGATGCTGAACCATTCATGAAGGATTCGCCCCCATGAT360.10949238115514463No Hit
ATTAATGGGAGATGTGGACAAAAATATCAGCATCAGTGATGTGACACCCAGT360.10949238115514463No Hit
TTAATGGGGCTTCTATTAAACTGCCGATTATCTATTCAATTTATTTCCAGCA360.10949238115514463No Hit
GACAATGGGATGTACAAAAATCTCAGGAATTTTTCTTTTCAGATATCTTAAA360.10949238115514463No Hit
GATGAGGGAACATGGTTTGTCTTTCTCTGTCACTGTACTTTTAATCTGTCTT360.10949238115514463No Hit
AGAAGAGGGGACTCACAGACAGTGATGAGATGAGGGTGGTGAGATCATGCAG360.10949238115514463No Hit
TGCTCGGGGCACGGACTGCCTGCAGTTCTACGTGAACAGCACACTTAAGGAA360.10949238115514463No Hit
GTATATGGGGGCAATTATTTTTAGATATGATATCAAAAGCGTAAGCAGCAAA360.10949238115514463No Hit
GTGCAGGGGTACAGAATGAATTGAGCAAAGATGGCCTAGCATTCGTATTTAG350.10645092612305727No Hit
CATAAGGGGGCTGCTAAGACAAATCAAAACCAAAAGCTGTAGATTCACAAAC350.10645092612305727No Hit
TTAGCTCGGGTTCTCACACATTGCCTGACAAGCTTGGATGCAAACAACCAGC350.10645092612305727No Hit
GCACAAGGGAAGATACAGATTATGAAACTAATTATAAACTTTACTGAAAATC350.10645092612305727No Hit
TCTAAGGGGATCTACGGCGGGATCACAAAGGGAAAATAACCTTTAAAAACAA350.10645092612305727No Hit
GCTTGTGGGTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAA350.10645092612305727No Hit
TTGGCGGGGGGAAATACGAAATTGCAAATCTAAGTGAAAAACCTTTGTGTAA350.10645092612305727No Hit
TTGGCTGGGGAAGTGTGGCAGTTTACTCTCTCCACTGTTGGAGAAGCCACCC350.10645092612305727No Hit
GACCTAGGGCAGATATATCAACATATTAGCATTAATTTTGACTTGATAAAAA350.10645092612305727No Hit
CTTTTGGGGAACCTCCATAACACCAGTGACTGCCTGTCTTTTTTCTTATAGA350.10645092612305727No Hit
TTCAACGGGCGATCAGCCAAGGGATGACCCCAATACGCAAAACTAACCCCCT350.10645092612305727No Hit
GCTCAGGGACGGCCAAGTTTCTTCTAGTATGATGGAAAGTTTCTTTTTTCTT350.10645092612305727No Hit
GACACCGGGGCCAGGAAGGCGAGTCCTCGCTGCATTTCTCCTCTCTGAGCAA340.10340947109096991No Hit
TGTTTTGGGTGGAGCACGATTTAAAAAGAGATCAGTGTTTTCCATTCTCTTT340.10340947109096991No Hit
TTACCAGGGACTGAATGTGAATTTTGCTGTTTTTTTAATAGAAAATAAAATA340.10340947109096991No Hit
AAAAAGGGGGAGGTCTATCGACCAAATATACTGCATGCATTTTACATTCATA340.10340947109096991No Hit
GACTATGGGACTATTTCCTCATTTATAAAATAAGGAAAATTATTCTTACCTC340.10340947109096991No Hit
AAACCTGGGAAGTTGATTAAATCTTCTGAAATATAAAAAATTAAGAAGCCAG340.10340947109096991No Hit
GCTGAGGGATTGAAAGACAGGAAACCTTAAAAGAAAAACAAAACTGATAATC340.10340947109096991No Hit
AGTAACGGGCCAAGCCTGTTTGTCTTCATTGTGACACTGGAGGTTTTGGATG340.10340947109096991No Hit
TCTGCTGGGATCAGAAACACACAATAATCCTATGTGTCTACGCACCTGAGAA340.10340947109096991No Hit
TTGGAAGGGTGGTCTCTTCCAACTTCATGTACCAGCGTAAAGATTAAAGTGG340.10340947109096991No Hit
TACTCAGGGGGTTATAAAAAGGAAAAGTTTCAAAAGGGTATATTTTTCTATA340.10340947109096991No Hit
CGAACTGGGCTCGTTGCCATCTTAGTTTTGATGGGTTTTGGCTAGCGTCTTT340.10340947109096991No Hit
GTACGAGGGTGTCCAGCACACACCTCAGTAGTAGGAGATATGGGTCTTTGGA340.10340947109096991No Hit
ATTCTCGGGGAAATCTCTGTTTAAGATCTCCACTTACAGTTTCTTACGGAAT340.10340947109096991No Hit
TTCACAGGGATTATCTCAATTAATCCTCACAATCAAATTCTGAGGTAGTTAC340.10340947109096991No Hit
GTTTGAGGGCTGGTTGCGGAAATTTCAGCTTCTATATATTGTATGTATATAT340.10340947109096991No Hit
CTATACGGGGACGCCGGGGAATGACAAGTGGTAGATGACCTACTTGTGCAGA340.10340947109096991No Hit
GGTATAGGGACTGGTCAGCTCCTTCGGACAAGGAAGGGTACAGTGGCGTGGG340.10340947109096991No Hit
GATCACGGTGACAAAAAGAGCTTGTCAGAACTAATTTCTCCATCTTCCCTCC340.10340947109096991No Hit
GTATCCGGGATGTGCTGTTTTTGAAGCATTTTTAAAAACGAATTGTAGTTGT330.10036801605888257No Hit
CACGCAAGGGAATCTTCTGACTTAAAAAGATAAAATGAGTATTTGTGATTTG330.10036801605888257No Hit
ACATTTGGGCGAGATGAGCTGCACACAGAGCAGGATGCCATGCTGGTGGACA330.10036801605888257No Hit
ACTCATGGGTGCCACAGCTTGTTCGCAGTTTACACAAACTCAGCTTTAAAAT330.10036801605888257No Hit
TACGCTGGGGTACTGCAATGGTGGCCTACCTTTGCCTGTAATATTAGGCATA330.10036801605888257No Hit
CGACAGGGGTATCTAGATAGGATCATCAGAGGAAATCAACTTCAAGAATTTG330.10036801605888257No Hit
TTATAGGGGAAGCAGATGTAAGAACATTATAAAGATAAAAGAAGAAATAAAT330.10036801605888257No Hit
TTAACAGGGAGGCGTGCGAGGAGGGGGCATGGCGGATACTGTGTTGTTTGAG330.10036801605888257No Hit
CTCACCAGGGCCTTTACCAAGTACTGCAAGAAATGGCAGGATGAGGATGGCA330.10036801605888257No Hit
ATCTCTGGGACTACATAATTTGGTGTCATGCTCTTTTAAGCAAATCTGAGTA330.10036801605888257No Hit
TAGTGGGAAATGAAATAATGAAATGAGATGAAATAAAATAATGAAATGATGA330.10036801605888257No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)