FastQCFastQC Report
Sat 31 Dec 2022
SRR5845282.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845282.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125253
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT3040.2427087574748709Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC2990.23871683712166575No Hit
ATCAATGGGATTAGAAGACAGTTTGGATGTTTCCTACAAAACTAAACACTTT2520.20119278580153768No Hit
AACTTAGGGATCTCTGAATATTTTCCTAGAAAATATGCTACCAGCTAAAGCA2370.18921702474192234No Hit
AACAAAGGGACTGGTCAGACATGCTGGCCAACCCCAGGCGACCTATTGCCCA2290.18282995217679415No Hit
GTTGCCGGGGAGTTGCTTCAGCGTCCCGGTGTGGCTGTGCCGTTGGTCCTGT2270.1812331840355121No Hit
CATACCAGGGGAGGAAGAGAGGAGAGCTGAACTTCGGGCAAACGGCAAAGTC2180.17404772739974292No Hit
GCCTTTGGGAACCTCTGTGAACCTCAGTTTACAGATCAGGAAAGGACTATAC2120.16925742297589677No Hit
TTGTCTGGGATTCACAAACAATTCCAGAAGAAATAGTTGATATTTTTATGTG2060.16446711855205065No Hit
ATGTTAGGGGCTATTCCCAGTTGGAGAGATGAGCAAATATTTCTATGTAAGC2040.16287035041076858No Hit
GCACTGGGACCCTGTCAGAAACCAGCACTGGGGGCCCTGCCACCCCCACATG2010.16047519819884554No Hit
TTAATAGGGACAGAAGCTGAAGTTTTCTGGGAGATGTGTAGAATGATGTGTC1990.1588784300575635No Hit
CGATATGGGGTAAATCAATTTACAACAAACTGAACAAGGCAATTGTGTTTAC1980.1580800459869225No Hit
GTGTCCGGGCGCTCTTCTGATCTCTTGTAGGGGATCTGTTTCATTAAATTTG1980.1580800459869225No Hit
GTTTCTGGGAGCCCTGTCCAGCCCGGTTCCCGGGAGGATGAAGTTCGTGTAC1960.15648327784564042No Hit
ATCCCCCGTTTTTTGGTCATGTTTCAATTAATTGTGAGGAAGGCGCAGCTCC1940.15488650970435838No Hit
ACTTTAGGGTATGGGCAAAATAGAAACAGAGAATGAGAAAAAAATAAGAAAA1910.1524913574924353No Hit
GCATTGGGGATTCAGGGAAGTAGTTAAGAGGCTAGGCTTTCAATTCAAACAT1880.15009620528051223No Hit
CGCACTGGGATCAGTGGCCAGTTTTGCATTTCTAGACAAAGAAGCATAGTGC1880.15009620528051223No Hit
CATCGCTGGGGTCAACACTGATAAGGTCCAAATAATTTGTATTTGAAACATC1870.1492978212098712No Hit
TTTGCAGGGTGGCTGGTCGTGGTATTGACATCCAAGATGTGTCTATGGTTGT1870.1492978212098712No Hit
CTAAGCGGGACCCTGATGCCAAAACCAAACAAAGACAACACAAGAGAAGAAG1850.14770105306858916No Hit
GGCTATGGGGTAAACTTTGCCCTCCTTGAGAAATATGGAACTACCTTAGAGG1850.14770105306858916No Hit
CGTTGGGGGAATCTGAAGTCTCCTATTTGATCAGGTTATTTCCTGGGACTTG1840.14690266899794815No Hit
GCTATGGGTGGCAGGACGTCCTGCCCTTCTGGGGAGTTCAAAATGGCTTTCT1840.14690266899794815No Hit
TCAGCTGGGAGACCGGCTGGCCGCGCGCGGGAAGCCGCGGAACTGCGCCGGA1830.14610428492730712No Hit
GCGTATGGGGTCCCACACGTGGGGGGTGGTCACCCCCCTTCACTCCAGCCCG1820.14530590085666611No Hit
TAAGAAGGGGATCTAAGCATGGGACTTGTACGGACTTTGAAGTGATTCAGTT1820.14530590085666611No Hit
TCATGCGGGAGAGACTTATGTACAAGGACCTTCAATTTTACTTGAAGTAGTT1820.14530590085666611No Hit
ATACATGGGAAGAGTAAAACTGCCCTTATAAAAAACAAGACAGAAATCTCAA1820.14530590085666611No Hit
AGGCCAGGGACATAGACTTCAAAATAAAGCATAAATAGAAGTGCATGGAAAG1820.14530590085666611No Hit
TATATAGGGAGTCTACCCTCCCTTAGCAGGGAACTACTCCCACCCTGGAGCC1810.14450751678602508No Hit
TAACAGGGATCTCTGACTTAGATACGTAAATTGTTGCAGTTAGAGCACTTTG1800.14370913271538407No Hit
ATCCTCGGGATCAATGTTGTGTTAGAAGATGAAACAAATCAATGAACAATCT1790.14291074864474304No Hit
TTTTTGGGGGAGTAGAGGAAGGTCGTCTGATCTTTGATAACTTGAAGAAATC1780.142112364574102No Hit
TGAGTGGGGGAACAAGATGGTAATGCAGTTGCCTTTGTTTATTTAAATAGAA1770.141313980503461No Hit
TATCGCGGGATAAAAGTATTGTTTTATGTAGTTACCTACCAATACTATCTGC1770.141313980503461No Hit
CAATACGGGACATGTATTTATTCAGAAATATGTGCACCCAGACTACTTTCTG1770.141313980503461No Hit
ATGTAGGGGATCTGGTTGGTTTTCAGCCTAGAATTTGATACGCTTTTTAGAA1760.14051559643281997No Hit
GCCTTGGGAGAGGCATGACTCTGTGAATGGCTTCTGGCATTAAATAGCCTGG1740.13891882829153793No Hit
CTTTCAGGGGGCTCCCCCTCGGAAAGCTGCGAAAGTGCTTTGGCGGTTTGTC1740.13891882829153793No Hit
AAACGAGGGATTTGTGTCATGAGCATTTGTTTCAAAGCAGCAATTACATCAT1730.13812044422089692No Hit
TTGCAGGAGAGAGAAAAGCTGAAAAAATGCAAAGAATAACCACATACTTTCC1700.13572529200897382No Hit
CTCGTGGGGACTAAATTTGAGGTGAGGTGTCTTCAGCTTCATGGTGTCCACA1700.13572529200897382No Hit
TGTAAAGGGAACATATACATAAACATAGAATAAACATATATATAATTGATTA1690.1349269079383328No Hit
CTTGCCGGGGAGGTTGGTCTGGACCGGAAGCGAAGATGGCGACTTCTGGCGC1690.1349269079383328No Hit
GCATTAGGGATGTATCAATAATATATAACAATTTAAAAATGGGAAAAAGACT1690.1349269079383328No Hit
AGCCTGGGTGACAGAGCGAGACTCCCTCTTAATAAAAAATAAAATAAAATAA1670.13333013979705077No Hit
GAGTAAGGGACTAAAGTAAAACATTATTAGAAATTAGCCTAAAGACTTTGAG1650.13173337165576873No Hit
GCTGTCGGGAATAAAGCCAACTGGAAACGAATCGGAGCCATTTTCATTTTCC1650.13173337165576873No Hit
ATGTCTGGGAGCTTCAAAATGATATTGAAATCCACGTATCCTATATTTTGAA1640.1309349875851277No Hit
TGTGCAGGGGAATGTAATTATACAGTATTTGTCCTTTTGTAACGGGCTCATT1640.1309349875851277No Hit
GTGATAGGGATTTACTGGAACTGAAAGTTCCAGTTGGGATGAAGCATTTCAT1640.1309349875851277No Hit
TGAACAGGGAGGGGCCAACTGACACCTCACACGGCCAGGTACTCTTCTGAGA1640.1309349875851277No Hit
AGAAGTGGCTCTCTAAACTAGAAGCCTTTTATAACTTTCCTTTCATAAAAGG1630.1301366035144867No Hit
AATTGTGGGATATGAAAATACCATTAAGTCCAATATAACTGGAAGTTTAGGA1630.1301366035144867No Hit
CTTGTTGGGTATAATAGGCACTCAATTTATTATAAATCTTCAGTATGTCCTG1630.1301366035144867No Hit
GAGATAGGGCCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1630.1301366035144867No Hit
TACAGAGGGGTTGTAGACTGGCTGCTGCTGTACGTGACTGTGATCAAAGTCA1630.1301366035144867No Hit
AGCTCGGGACAGTACTACAGGTGCATACTCTGGTCATTTTTCAAGCCATGTT1630.1301366035144867No Hit
ATTGCCGGCACCTTGGGGCATCAAAAATAAAATGTAGCGGGGGAAGTATAAG1620.12933821944384566No Hit
CAATTAGGGCTGACAATGAGTGAAAAGCGGGAATTTCCTACAGTGCCCTTGG1610.12853983537320463No Hit
GCAAAGGGATCCAATTTAAGAATAATACCATACAATAAAGTTCAGAATATAT1600.1277414513025636No Hit
AACCCAGCACAGACCCAGTAGTGATGGTCACAGGGCTGCTTGTGTCACCACT1600.1277414513025636No Hit
TAGTTAGGGTCTGCCACTTCCTCTAGTTTGTCCTTAAAAAAGCAAGAGCAAT1600.1277414513025636No Hit
ACGTCCGGGGACCCCGAGTGTGACTGGGAAGGAGAACTTATTCCTTACCAGG1590.12694306723192259No Hit
AGTCTTGGGCCTGTACTTAGTCACGGGGATGGTGGAAGAGGGAGAAGAGGAA1590.12694306723192259No Hit
TCTTTAGGGGTATTGCTGTGCTCAACAAAGCCAGTTTAGTATACTGTGACTT1590.12694306723192259No Hit
AAGTCGGGGATTTGCCTATCCTAGGTATTTTATTTAAGTGGAGTCATACAGT1580.12614468316128155No Hit
CGAGGGGATGTCATGAAGGCCAGAGGGTCTCTGTCATTTTTATCCTTCTATG1570.12534629909064054No Hit
GTAGGCGGGGCAGCGGGCGATGACGTAGAGGGACGTGCCCTCTATATGAGGT1570.12534629909064054No Hit
GATACCGGGGGACAAGTAAAGTTTGAATCAGCTTCTCCATGGCCTGGGCACC1560.12454791501999952No Hit
AAACAAGGGAGGCGCCTGCCACCATGCCTAGCTAATTTTTGTATTTTGAGTA1560.12454791501999952No Hit
TTCTTAGGGAACGTGCAAAAATGACCACCAGTCCTATACACCAATAATAGAA1550.12374953094935849No Hit
TTATTAGGGTACTTATTTGAAGGTAAAGATTATTCTAAAGAGCCCAGTAAGG1550.12374953094935849No Hit
TCGCATGGGATCACCGGGTTCATTACCGGCCAGAGATGGACGGGGAGAAGCA1550.12374953094935849No Hit
CTCAACCGGGCAGGCAGAGCTGTTCCTCTGCTATTGTTGCTGCGTTTCTTTT1550.12374953094935849No Hit
GTACTTGGGGCAAGAAATTGCCCGGAGTTTTGGAAAACTGATGAGTAAAGGC1550.12374953094935849No Hit
ATATATGGGAGAGTGCTGAGATTACAGGTGAGAGCCACCATGCCTGGCCTAT1550.12374953094935849No Hit
GCTGCGGGGTCCGATCTCTTCTGGGGATTTTTAAATATACACAAGACATTTG1540.12295114687871748No Hit
GCGGTTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1530.12215276280807645No Hit
CGCAACGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1530.12215276280807645No Hit
TTGCTGGGATTACAGGTGTGAGTCACTGTGCCCAGCCACCTCTTTTTAATAT1530.12215276280807645No Hit
CGTTGGGGGAACTGGAACAGTTCTAAAATTTATCTGATACTTCTCTAACAAG1520.12135437873743544No Hit
CTCTTTGGGCTCAGCTGGCTAATGGAGAAAATGTGAGGAATTACCTGTCAAA1510.12055599466679441No Hit
CAGTACGGGCTTCCGATCTCGCTGTGGGCTTCAAGACTTTCTTTGCCTCTCT1500.11975761059615339No Hit
GTTAAGGGGGTGTAAGAATTAAGAGAAGCCAAAGCTACTGGGAATGAACAAA1500.11975761059615339No Hit
ATAGTAGGGATAGCTCACTCCAGCCTCCAACTCCTGGGCTCAAGCAATCCTC1500.11975761059615339No Hit
ATTGCAGGGAACACCCACTCATGATAGAAACTAGTAAACTAGGAATAGAGGG1490.11895922652551236No Hit
GTCTAAGGGTCCAAGGCTATAGTGATCAAACCAGCGTGATACTGGTATAAAA1490.11895922652551236No Hit
GTCCAGGGAAAGTGATTCTGGGAAACTATACTTATTCTAGTAGCATTGCTCT1490.11895922652551236No Hit
CCTCCTGGGAGCTATGTTTTGGCATAAGAATAAAGAGTCTCTGTTTTAGAAG1480.11816084245487135No Hit
CTTTCTGGGGTCATATTGTGAATACTGCTGCAAAGCACGGGACTGCAGACGT1480.11816084245487135No Hit
TTTCAGGGAAGAAAAGAAAGGAAAAGTGAAGTAGGCATATGAAAGAAAAGGC1470.11736245838423032No Hit
TCAGGGGGATCTACTCCAGGGGAGCCATCATCTGTTTTGAATGGAGGAAAAT1470.11736245838423032No Hit
CAAAGCGGAATCTGGATTACCACAATCCTGACATTCCTGAGTGGAGAAAAGA1460.1165640743135893No Hit
CGTCAAGGGGGAAACATTCGCTTGTAGTTCCATTTTACTTGATCTCTACAAG1450.11576569024294826No Hit
AATTGCGGGACTTGTACGGACTTTGAAGTGATTCAGTTTCCTCTACGGATGA1430.11416892210166622No Hit
TTCTGTGGGGTTACATATTTTGAGAATCAAATTATTTTATAAAAGATATAAT1430.11416892210166622No Hit
AGCTATGGGTGTGAGGTCTGTGTTGTAAATCATCACCTCTATTCCCCAACTG1430.11416892210166622No Hit
GTCGCCGGGCGTGGTGATGCAGGATGAGTCCTTCCCTGCCTGCAAGATTGAG1430.11416892210166622No Hit
ATCGACGGGATTCTTTGTTAAAAGGGTGTGTAAGCTCTCAGACCTATCCACT1430.11416892210166622No Hit
CTGACCGGGATCTCAAAACAGTTATAAGATTGACAATATGATCCCTACTTTG1420.1133705380310252No Hit
ACAACAGGGAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA1420.1133705380310252No Hit
ATTCGCGGGAATATATTTTCATTGTGTCTTTATAGAAAAATTAAACAATAGA1420.1133705380310252No Hit
GACATTGGGATTCTAATAAAATTGTGTACTATCCTGTGTGCTTAGTTTTAAG1420.1133705380310252No Hit
GAATATGGGAACACATTTACTAACAATAGTAGACATTATTGAGTGTTTTCTG1410.11257215396038417No Hit
GACGCCGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG1410.11257215396038417No Hit
GCTCTGGGGTCTACTATGTAGAAGTGACTGAAAAGTCAATCAGAAAATGTGT1410.11257215396038417No Hit
CACAGAGGACCCAAACAAAACACATAAACTGTATTATGCCCTTCTTTTAGAT1410.11257215396038417No Hit
CTTCATGGGGTGGATTTTGTCCATTCATCTCATGCTCTCTGAACAGAAGGTA1400.11177376988974316No Hit
AGCCAAGGGTCCGATCTGCCGGGGATTTTATTTTATTCATGAGCTGAGATGA1400.11177376988974316No Hit
ACTAGGGAGTGAAAAAAATGTATCTTTTTGTTAAAACTGGCTTATTTGAATG1390.11097538581910213No Hit
GTCCGTGGGGGTTCAAATTTAACATGAGAGATTAAGAAATATATGTGATACA1390.11097538581910213No Hit
TATAATGGGATGAAGACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAG1380.11017700174846112No Hit
GCTGGTGGGGTTAAATTAATTTTTTTAATTATTATTTAAAGTAAAACTGTCA1380.11017700174846112No Hit
AAACAAGGGCACTGGCAGTGAGATCCGCCACCGTGATGATGCTCTACCTTTT1370.10937861767782009No Hit
TAAGTGGGAAGCTATTTCATAATAAATTCTGTACAGTTTCCCCCAAAAAAAG1370.10937861767782009No Hit
TGAGTGGGTGCTCTGGAACCTTTTCACAGGAATGAGAATTGAGGGTCTGAGT1370.10937861767782009No Hit
AAGTCGGGGGATCTAATGGTGGGGTTTGGAACTCTGAAGGATTTAGACAAGG1360.10858023360717907No Hit
TTTTAGGGGTGAAGCAAACCAATTATAATTAACCACATGTTGTAGTAACTGG1360.10858023360717907No Hit
GTTCTAGGGTACCAGTTTGGTTTTAGTTTGTCTTTGTCAGCGCCACGTGAGA1360.10858023360717907No Hit
ACGGCTGGGATGAAGTGAGATGTCATATCCTGAATATAGTTTGTCTTCCCCA1360.10858023360717907No Hit
TTGTCCGGGAATCCATATTGTGGAATGGTTATACTTCCCAAAGGACTGTATA1360.10858023360717907No Hit
CGCAAAGGGAGGTTAATAGAGGGTGGTATTTAGGAGATGTTGAAAGGCTGTG1350.10778184953653806No Hit
TGCATGCGGGACCAGAATCTCTGGGACACATTTAAAGCTGTGTGTCGACGGA1350.10778184953653806No Hit
TGGCGCTGGGAACACCAACAGTATATAAAGGTTCCCCTTTCTCCACATTCTC1340.10698346546589703No Hit
ATATACGGGGTGAACTGGATAGGTACCCCACACCTGGATGGGAAGTTACCTT1340.10698346546589703No Hit
GCGAAGGGGGGCAATTAATTGGATATATGAGTCTGGAACTCTGGGAAAGTTT1330.10618508139525601No Hit
TAGTAGGGGCTCTCCATTGGAAAAGGAGCTGTTGCAAACCGTGTTCATTTAA1330.10618508139525601No Hit
GCTGTAGGGAATCCACAGAGGAGCCTGCTGGTTCTAATCGGGGACAGTATCC1330.10618508139525601No Hit
GCTTCAGGGAGAACACATATAACAAAATAATAGGCTTAAATCTAACACATCA1330.10618508139525601No Hit
CCAGATTCGAGACTTGGAAAGAGGTGTTGAGATCTGCATAGCCACTCCTGGA1320.10538669732461498No Hit
ACTAACCGGGTATCTGATTCTTCTAACAAAACAACACTCTGAGAAAGGCATC1320.10538669732461498No Hit
AAAATGAGGGTGTTATCACCCAGAAGAAAATAGTGGTGTGATTTGGGGATAT1320.10538669732461498No Hit
ATACCGGGAGAATAGAAAACCCAGAAATAGATCCACACAAATATGCCTAATT1310.10458831325397397No Hit
TGTTCAGGGACAACCTGTATATTGTATGCATTGCTGCGTGGAGGCTGTTTAT1300.10378992918333294No Hit
TAACCTGGGAGAATTGCCTTTGATTTCAAATGGTAACAGCATTTATTGGATT1300.10378992918333294No Hit
CGCATAGGGGAAATCTAGTAGACATAACTTTAACCAGGCAATCAAGGTTAAT1300.10378992918333294No Hit
ATGCCTGGGAGGATGCTCACTCCCGGGCCCGAGCACGGTGGAGGCTTGGCTC1290.10299154511269192No Hit
GTTTATGGGAACTCAGTGAAGCCAGACTGTACTGTGGAGAAAGTAGAAATAT1290.10299154511269192No Hit
TTTGTTGGGTTTGAGAAGAAATGCTGCTGGCTCTATTTTTACATTCCCTTCC1290.10299154511269192No Hit
CACCACGGGAGCTTCCAGTGTGATCGTTGCAGAAGACGGGTGATTTCTGCAT1290.10299154511269192No Hit
GTTCTTGGGGAGCCCCGCGGGTGTCTGTGGAGGGGGCTCCGGTCCAGGTACT1290.10299154511269192No Hit
CAACTAGGGGTTTCTGATGGAAAATGGCGGTTTCAAGTCCCCGATTCCGTGC1290.10299154511269192No Hit
CTTCATGGGAGAGGTGATGGGAATGTGTCCCTGTTAACTGACTATATTTATT1280.10219316104205088No Hit
AACAGCCCAGCATCAGGGTCACTGTGGCAACAGCAGGCTCTAGGGGAATCCT1280.10219316104205088No Hit
TACAGCTGGGACGAGCCACCGCAACTGGCTGAGAATCTTTTTATTTGCTGAT1280.10219316104205088No Hit
GTGCAGGGGAGGCCTGTGGTGGAGAAGGACGTGCCGTGCCGCTGGGTTCTGA1280.10219316104205088No Hit
ACTGCTGGGATTGCAGACGTGAGCCACCGCGCCCGGCCCCAGAAACGATTCT1270.10139477697140988No Hit
TCTTTGGGAAGATCCTCCTGGCCAACTTCTTGGCCCAGACAGAGGCCCTGAT1270.10139477697140988No Hit
ACACGGGTGAGTGTCAGGCTCTGAATCTCTCAAAATGTGCCGTCTTTGGTTC1270.10139477697140988No Hit
AATTCGGGGCACTGGGCTGACGTCAAGCAGTATATTTCAGGATGATTTAGGA1270.10139477697140988No Hit
GTACATGGGGGATGGAAGGCCAGAGGAAGGCCGGGCTCAGCAGGCCCCGCAG1270.10139477697140988No Hit
AACACCGGGGAGGACATTTTATGAGTTTCAGTTATAATTATTCTAGCAAGAA1260.10059639290076884No Hit
CGTGACGGGGGAGAATTTACGCATTATTCGTGGGACAAAACTTTATGAGGAT1260.10059639290076884No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)