FastQCFastQC Report
Sat 31 Dec 2022
SRR5845281.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845281.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125099
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC3420.27338348028361537No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT3120.24940247324119297Illumina PCR Primer Index 5 (95% over 21bp)
GTTGCCGGGGAGTTGCTTCAGCGTCCCGGTGTGGCTGTGCCGTTGGTCCTGT2740.21902653098745795No Hit
AACTTAGGGATCTCTGAATATTTTCCTAGAAAATATGCTACCAGCTAAAGCA2570.20543729366341856No Hit
ATGTTAGGGGCTATTCCCAGTTGGAGAGATGAGCAAATATTTCTATGTAAGC2420.19344679014220736No Hit
ATCAATGGGATTAGAAGACAGTTTGGATGTTTCCTACAAAACTAAACACTTT2340.18705185493089474No Hit
AGAAGTGGCTCTCTAAACTAGAAGCCTTTTATAACTTTCCTTTCATAAAAGG2260.1806569197195821No Hit
TTGTCTGGGATTCACAAACAATTCCAGAAGAAATAGTTGATATTTTTATGTG2260.1806569197195821No Hit
CATACCAGGGGAGGAAGAGAGGAGAGCTGAACTTCGGGCAAACGGCAAAGTC2160.17266325070544128No Hit
CATCGCTGGGGTCAACACTGATAAGGTCCAAATAATTTGTATTTGAAACATC2120.16946578309978497No Hit
GTGTCCGGGCGCTCTTCTGATCTCTTGTAGGGGATCTGTTTCATTAAATTTG2120.16946578309978497No Hit
AACAAAGGGACTGGTCAGACATGCTGGCCAACCCCAGGCGACCTATTGCCCA2090.16706768239554273No Hit
GACATTGGGATTCTAATAAAATTGTGTACTATCCTGTGTGCTTAGTTTTAAG2060.16466958169130047No Hit
GTTTCTGGGAGCCCTGTCCAGCCCGGTTCCCGGGAGGATGAAGTTCGTGTAC2040.16307084788847234No Hit
CAATACGGGACATGTATTTATTCAGAAATATGTGCACCCAGACTACTTTCTG2040.16307084788847234No Hit
CTAAGCGGGACCCTGATGCCAAAACCAAACAAAGACAACACAAGAGAAGAAG2000.159873380282816No Hit
GCCTTTGGGAACCTCTGTGAACCTCAGTTTACAGATCAGGAAAGGACTATAC1990.15907401338140192No Hit
TAACAGGGATCTCTGACTTAGATACGTAAATTGTTGCAGTTAGAGCACTTTG1940.15507717887433153No Hit
CGCACTGGGATCAGTGGCCAGTTTTGCATTTCTAGACAAAGAAGCATAGTGC1930.15427781197291746No Hit
AATTGCGGGACTTGTACGGACTTTGAAGTGATTCAGTTTCCTCTACGGATGA1920.15347844507150335No Hit
AAGTCGGGGATTTGCCTATCCTAGGTATTTTATTTAAGTGGAGTCATACAGT1900.15187971126867522No Hit
TATCGCGGGATAAAAGTATTGTTTTATGTAGTTACCTACCAATACTATCTGC1890.15108034436726112No Hit
TTTCAGGGAAGAAAAGAAAGGAAAAGTGAAGTAGGCATATGAAAGAAAAGGC1880.15028097746584707No Hit
TTCTTAGGGAACGTGCAAAAATGACCACCAGTCCTATACACCAATAATAGAA1880.15028097746584707No Hit
CTTGCCGGGGAGGTTGGTCTGGACCGGAAGCGAAGATGGCGACTTCTGGCGC1880.15028097746584707No Hit
TTAATAGGGACAGAAGCTGAAGTTTTCTGGGAGATGTGTAGAATGATGTGTC1870.14948161056443296No Hit
GGCTATGGGGTAAACTTTGCCCTCCTTGAGAAATATGGAACTACCTTAGAGG1870.14948161056443296No Hit
AAACGAGGGATTTGTGTCATGAGCATTTGTTTCAAAGCAGCAATTACATCAT1860.14868224366301888No Hit
CGATATGGGGTAAATCAATTTACAACAAACTGAACAAGGCAATTGTGTTTAC1860.14868224366301888No Hit
ACTTTAGGGTATGGGCAAAATAGAAACAGAGAATGAGAAAAAAATAAGAAAA1860.14868224366301888No Hit
TATATAGGGAGTCTACCCTCCCTTAGCAGGGAACTACTCCCACCCTGGAGCC1840.14708350986019073No Hit
CGTTGGGGGAACTGGAACAGTTCTAAAATTTATCTGATACTTCTCTAACAAG1840.14708350986019073No Hit
ATAGTAGGGATAGCTCACTCCAGCCTCCAACTCCTGGGCTCAAGCAATCCTC1820.14548477605736257No Hit
ATACATGGGAAGAGTAAAACTGCCCTTATAAAAAACAAGACAGAAATCTCAA1820.14548477605736257No Hit
TTGCAGGAGAGAGAAAAGCTGAAAAAATGCAAAGAATAACCACATACTTTCC1810.1446854091559485No Hit
TAAGAAGGGGATCTAAGCATGGGACTTGTACGGACTTTGAAGTGATTCAGTT1790.1430866753531203No Hit
GCACTGGGACCCTGTCAGAAACCAGCACTGGGGGCCCTGCCACCCCCACATG1790.1430866753531203No Hit
TTTTTGGGGGAGTAGAGGAAGGTCGTCTGATCTTTGATAACTTGAAGAAATC1790.1430866753531203No Hit
GAGTAAGGGACTAAAGTAAAACATTATTAGAAATTAGCCTAAAGACTTTGAG1790.1430866753531203No Hit
AGTCTTGGGCCTGTACTTAGTCACGGGGATGGTGGAAGAGGGAGAAGAGGAA1770.14148794155029218No Hit
GCTGCGGGGTCCGATCTCTTCTGGGGATTTTTAAATATACACAAGACATTTG1760.14068857464887807No Hit
ATTGCAGGGAACACCCACTCATGATAGAAACTAGTAAACTAGGAATAGAGGG1760.14068857464887807No Hit
TCAGCTGGGAGACCGGCTGGCCGCGCGCGGGAAGCCGCGGAACTGCGCCGGA1760.14068857464887807No Hit
CCTCCTGGGAGCTATGTTTTGGCATAAGAATAAAGAGTCTCTGTTTTAGAAG1710.13669174014180768No Hit
GCCTTGGGAGAGGCATGACTCTGTGAATGGCTTCTGGCATTAAATAGCCTGG1710.13669174014180768No Hit
TTTGCAGGGTGGCTGGTCGTGGTATTGACATCCAAGATGTGTCTATGGTTGT1710.13669174014180768No Hit
TCGCATGGGATCACCGGGTTCATTACCGGCCAGAGATGGACGGGGAGAAGCA1670.13349427253615137No Hit
GCGGTTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1660.1326949056347373No Hit
AATTGTGGGATATGAAAATACCATTAAGTCCAATATAACTGGAAGTTTAGGA1660.1326949056347373No Hit
AAACAAGGGAGGCGCCTGCCACCATGCCTAGCTAATTTTTGTATTTTGAGTA1660.1326949056347373No Hit
TCATGCGGGAGAGACTTATGTACAAGGACCTTCAATTTTACTTGAAGTAGTT1660.1326949056347373No Hit
GTAGGCGGGGCAGCGGGCGATGACGTAGAGGGACGTGCCCTCTATATGAGGT1660.1326949056347373No Hit
CGCATAGGGGAAATCTAGTAGACATAACTTTAACCAGGCAATCAAGGTTAAT1660.1326949056347373No Hit
GTCCAGGGAAAGTGATTCTGGGAAACTATACTTATTCTAGTAGCATTGCTCT1660.1326949056347373No Hit
GCAAAGGGATCCAATTTAAGAATAATACCATACAATAAAGTTCAGAATATAT1650.13189553873332321No Hit
TACAGAGGGGTTGTAGACTGGCTGCTGCTGTACGTGACTGTGATCAAAGTCA1650.13189553873332321No Hit
GCATTAGGGATGTATCAATAATATATAACAATTTAAAAATGGGAAAAAGACT1650.13189553873332321No Hit
ATCCTCGGGATCAATGTTGTGTTAGAAGATGAAACAAATCAATGAACAATCT1650.13189553873332321No Hit
CTTTCAGGGGGCTCCCCCTCGGAAAGCTGCGAAAGTGCTTTGGCGGTTTGTC1640.13109617183190914No Hit
AAGTCGGGGGATCTAATGGTGGGGTTTGGAACTCTGAAGGATTTAGACAAGG1630.13029680493049506No Hit
TGGCGCTGGGAACACCAACAGTATATAAAGGTTCCCCTTTCTCCACATTCTC1630.13029680493049506No Hit
TTATTAGGGTACTTATTTGAAGGTAAAGATTATTCTAAAGAGCCCAGTAAGG1620.12949743802908095No Hit
GCTATGGGTGGCAGGACGTCCTGCCCTTCTGGGGAGTTCAAAATGGCTTTCT1610.1286980711276669No Hit
TCTTTAGGGGTATTGCTGTGCTCAACAAAGCCAGTTTAGTATACTGTGACTT1610.1286980711276669No Hit
GCTGGTGGGGTTAAATTAATTTTTTTAATTATTATTTAAAGTAAAACTGTCA1610.1286980711276669No Hit
CAAAGCGGAATCTGGATTACCACAATCCTGACATTCCTGAGTGGAGAAAAGA1600.1278987042262528No Hit
AGCTCGGGACAGTACTACAGGTGCATACTCTGGTCATTTTTCAAGCCATGTT1600.1278987042262528No Hit
AACCCAGCACAGACCCAGTAGTGATGGTCACAGGGCTGCTTGTGTCACCACT1590.12709933732483872No Hit
ATCCCCCGTTTTTTGGTCATGTTTCAATTAATTGTGAGGAAGGCGCAGCTCC1590.12709933732483872No Hit
ATCGACGGGATTCTTTGTTAAAAGGGTGTGTAAGCTCTCAGACCTATCCACT1580.12629997042342467No Hit
CAGTACGGGCTTCCGATCTCGCTGTGGGCTTCAAGACTTTCTTTGCCTCTCT1570.12550060352201056No Hit
GTGATAGGGATTTACTGGAACTGAAAGTTCCAGTTGGGATGAAGCATTTCAT1570.12550060352201056No Hit
AAACAAGGGCACTGGCAGTGAGATCCGCCACCGTGATGATGCTCTACCTTTT1550.12390186971918242No Hit
CTCGTGGGGACTAAATTTGAGGTGAGGTGTCTTCAGCTTCATGGTGTCCACA1550.12390186971918242No Hit
TGTGCAGGGGAATGTAATTATACAGTATTTGTCCTTTTGTAACGGGCTCATT1550.12390186971918242No Hit
TGTAAAGGGAACATATACATAAACATAGAATAAACATATATATAATTGATTA1540.12310250281776833No Hit
GATACCGGGGGACAAGTAAAGTTTGAATCAGCTTCTCCATGGCCTGGGCACC1540.12310250281776833No Hit
ACGTCCGGGGACCCCGAGTGTGACTGGGAAGGAGAACTTATTCCTTACCAGG1530.12230313591635425No Hit
GTACTTGGGGCAAGAAATTGCCCGGAGTTTTGGAAAACTGATGAGTAAAGGC1530.12230313591635425No Hit
AACAGCCCAGCATCAGGGTCACTGTGGCAACAGCAGGCTCTAGGGGAATCCT1520.12150376901494016No Hit
TAGACGGGGATGATGATTGAAATGAAAGCAGTTAATCTAAATCTTCAAATGT1520.12150376901494016No Hit
AGCTATGGGTGTGAGGTCTGTGTTGTAAATCATCACCTCTATTCCCCAACTG1520.12150376901494016No Hit
TGAACAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1520.12150376901494016No Hit
ACTAGGGAGTGAAAAAAATGTATCTTTTTGTTAAAACTGGCTTATTTGAATG1510.12070440211352608No Hit
ATATCAGGGACTAGAGTCATGGTAGTGGGTAAAAGCCAAAGAGCTCAGTTTC1510.12070440211352608No Hit
CTCTTTGGGCTCAGCTGGCTAATGGAGAAAATGTGAGGAATTACCTGTCAAA1510.12070440211352608No Hit
CGAGGGGATGTCATGAAGGCCAGAGGGTCTCTGTCATTTTTATCCTTCTATG1500.11990503521211202No Hit
TCAGGGGGATCTACTCCAGGGGAGCCATCATCTGTTTTGAATGGAGGAAAAT1500.11990503521211202No Hit
AGGCCAGGGACATAGACTTCAAAATAAAGCATAAATAGAAGTGCATGGAAAG1500.11990503521211202No Hit
GCTTCAGGGAGAACACATATAACAAAATAATAGGCTTAAATCTAACACATCA1500.11990503521211202No Hit
GCATTGGGGATTCAGGGAAGTAGTTAAGAGGCTAGGCTTTCAATTCAAACAT1490.11910566831069792No Hit
CGTTGGGGGAATCTGAAGTCTCCTATTTGATCAGGTTATTTCCTGGGACTTG1490.11910566831069792No Hit
TTGCTGGGATTACAGGTGTGAGTCACTGTGCCCAGCCACCTCTTTTTAATAT1490.11910566831069792No Hit
TGAGTGGGTGCTCTGGAACCTTTTCACAGGAATGAGAATTGAGGGTCTGAGT1490.11910566831069792No Hit
GCTGTCGGGAATAAAGCCAACTGGAAACGAATCGGAGCCATTTTCATTTTCC1490.11910566831069792No Hit
CTCAACCGGGCAGGCAGAGCTGTTCCTCTGCTATTGTTGCTGCGTTTCTTTT1480.11830630140928385No Hit
ACAACAGGGAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA1470.11750693450786978No Hit
TGCATGCGGGACCAGAATCTCTGGGACACATTTAAAGCTGTGTGTCGACGGA1460.11670756760645568No Hit
GCTCTGGGGTCTACTATGTAGAAGTGACTGAAAAGTCAATCAGAAAATGTGT1460.11670756760645568No Hit
TAGTTAGGGTCTGCCACTTCCTCTAGTTTGTCCTTAAAAAAGCAAGAGCAAT1460.11670756760645568No Hit
CTTCATGGGGTGGATTTTGTCCATTCATCTCATGCTCTCTGAACAGAAGGTA1450.11590820070504161No Hit
CGTGACGGGGGAGAATTTACGCATTATTCGTGGGACAAAACTTTATGAGGAT1450.11590820070504161No Hit
CGCAACGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1450.11590820070504161No Hit
TAGTAGGGGCTCTCCATTGGAAAAGGAGCTGTTGCAAACCGTGTTCATTTAA1440.11510883380362752No Hit
TTATAAGGGAAGGTAACTTTGAAAATAACCTCTTTAACATTTAATAATTTAA1440.11510883380362752No Hit
GTCGCCGGGCGTGGTGATGCAGGATGAGTCCTTCCCTGCCTGCAAGATTGAG1440.11510883380362752No Hit
CTTTCTGGGGTCATATTGTGAATACTGCTGCAAAGCACGGGACTGCAGACGT1430.11430946690221344No Hit
ATATATGGGAGAGTGCTGAGATTACAGGTGAGAGCCACCATGCCTGGCCTAT1430.11430946690221344No Hit
TACAGCTGGGACGAGCCACCGCAACTGGCTGAGAATCTTTTTATTTGCTGAT1420.11351010000079938No Hit
GCGAAGGGGGGCAATTAATTGGATATATGAGTCTGGAACTCTGGGAAAGTTT1420.11351010000079938No Hit
ATTCGCGGGAATATATTTTCATTGTGTCTTTATAGAAAAATTAAACAATAGA1420.11351010000079938No Hit
GCGTATGGGGTCCCACACGTGGGGGGTGGTCACCCCCCTTCACTCCAGCCCG1410.11271073309938529No Hit
AATTCGGGGCACTGGGCTGACGTCAAGCAGTATATTTCAGGATGATTTAGGA1410.11271073309938529No Hit
GTCTAAGGGTCCAAGGCTATAGTGATCAAACCAGCGTGATACTGGTATAAAA1410.11271073309938529No Hit
CTTCATGGGAGAGGTGATGGGAATGTGTCCCTGTTAACTGACTATATTTATT1400.11191136619797121No Hit
GTCCGTGGGGGTTCAAATTTAACATGAGAGATTAAGAAATATATGTGATACA1400.11191136619797121No Hit
CATTTGGGGACTCAGCACTCCTGGAAGTGTCTGGATACAGCTCACTGAGAGT1390.11111199929655713No Hit
TTAAATGGGATCCATAAGAATGTTTACACATGGCAGTATCAAAGCATATAAA1380.11031263239514304No Hit
ATGTAGGGGATCTGGTTGGTTTTCAGCCTAGAATTTGATACGCTTTTTAGAA1380.11031263239514304No Hit
CTGACCGGGATCTCAAAACAGTTATAAGATTGACAATATGATCCCTACTTTG1370.10951326549372897No Hit
AAAATGAGGGTGTTATCACCCAGAAGAAAATAGTGGTGTGATTTGGGGATAT1370.10951326549372897No Hit
GTTAAGGGGGTGTAAGAATTAAGAGAAGCCAAAGCTACTGGGAATGAACAAA1360.1087138985923149No Hit
AACAGTGGGTGAGACAGGGAGGCTATTTTTTAATATAGGATTTAGAAACCAA1360.1087138985923149No Hit
TATAATGGGATGAAGACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAG1360.1087138985923149No Hit
GAGATAGGGCCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1350.1079145316909008No Hit
TCACAAGGGCAACCTGAAGAAACAGGGCCTGCTGCCTCTGACCTTCGCTGAC1350.1079145316909008No Hit
AATAATGGGGTGGTGGCTTGCACCTCTGGTCTCAGCTACTTGGGATGCTAAG1350.1079145316909008No Hit
GACCCGGGGGTAAATAGAATCCTAGATGAATCCTAGAAAAATAATCCCTAAG1350.1079145316909008No Hit
CGTCAAGGGGGAAACATTCGCTTGTAGTTCCATTTTACTTGATCTCTACAAG1350.1079145316909008No Hit
AGCCAAGGGTCCGATCTGCCGGGGATTTTATTTTATTCATGAGCTGAGATGA1350.1079145316909008No Hit
TGAACAGGGAGGGGCCAACTGACACCTCACACGGCCAGGTACTCTTCTGAGA1350.1079145316909008No Hit
CGATATGGGAGAAAGATTTTAAAAAACTAACGTGCAGCAATTTCCAGTGAAC1340.10711516478948674No Hit
CAATTAGGGCTGACAATGAGTGAAAAGCGGGAATTTCCTACAGTGCCCTTGG1340.10711516478948674No Hit
GCTGTAGGGAATCCACAGAGGAGCCTGCTGGTTCTAATCGGGGACAGTATCC1330.10631579788807263No Hit
CTGTCTAGGGGAAGATCTTGATGCTTTGAAACTGTATGAGAGGTGTTGTTAT1330.10631579788807263No Hit
AGCCTGGGTGACAGAGCGAGACTCCCTCTTAATAAAAAATAAAATAAAATAA1330.10631579788807263No Hit
TTCTGTGGGGTTACATATTTTGAGAATCAAATTATTTTATAAAAGATATAAT1320.10551643098665857No Hit
GTTCTTGGGGAGCCCCGCGGGTGTCTGTGGAGGGGGCTCCGGTCCAGGTACT1320.10551643098665857No Hit
TCTGCTAGGGGGAGCCTGAGCTTGACCTCAAACGGGTGGCGATTTGATGGGT1320.10551643098665857No Hit
TTTTAGGGGTGAAGCAAACCAATTATAATTAACCACATGTTGTAGTAACTGG1320.10551643098665857No Hit
TCGAGCGGGATGGCAAAAGGGACTTTGCAAATGTATTAAAGTAAGGATATTG1320.10551643098665857No Hit
TTGAATGGGAGTCCCTACCCTGGAAGTACCGGCACCAGAAGCCGTATCCTCA1310.10471706408524449No Hit
CTTGTTGGGTATAATAGGCACTCAATTTATTATAAATCTTCAGTATGTCCTG1310.10471706408524449No Hit
CATGAGGGGGGGCGAAAGGCATCCCAAGAGGAGACAGTAACGTGTGTATAAA1300.1039176971838304No Hit
AACACCGGGGAGGACATTTTATGAGTTTCAGTTATAATTATTCTAGCAAGAA1300.1039176971838304No Hit
CACAGAGGACCCAAACAAAACACATAAACTGTATTATGCCCTTCTTTTAGAT1300.1039176971838304No Hit
TTTCTAGGGATAAGCGTTCATGGAACGCGTTGAAATTGTAATTATATCTGAG1290.10311833028241633No Hit
CAACTAGGGGTTTCTGATGGAAAATGGCGGTTTCAAGTCCCCGATTCCGTGC1290.10311833028241633No Hit
ATACCGGGAGAATAGAAAACCCAGAAATAGATCCACACAAATATGCCTAATT1290.10311833028241633No Hit
AATTGGGGCTTTTAAGAGGTCTGCCTGGTTCTGGGAAAACAACATTGTCTCG1280.10231896338100226No Hit
TCAAGCAGGGGAATGTGAAGAATGGGAAAATATATTTTTGCATGAAAACAGA1280.10231896338100226No Hit
TGAGTGGGGGAACAAGATGGTAATGCAGTTGCCTTTGTTTATTTAAATAGAA1280.10231896338100226No Hit
AAAATTGGGGTGTCATTGAGGAAAATTTCTATCATTTCTTGTCAACTACCAC1280.10231896338100226No Hit
ACTAAGGGGAGGACGGGCGCGTCTTCAGGGTGGAAGCCTGGCGCACGTCCGG1280.10231896338100226No Hit
TTGTAAGGGCAGGAATGGGCCAAGCCAGGGTTCCAGACAGGGTGGGGCTTAG1260.10072022957817409No Hit
TTCTCGCAGGGACTGTGCAATAACTGATTCATTTTTTTCAGAGCTTGAAGCA1260.10072022957817409No Hit
TTGTCCGGGAATCCATATTGTGGAATGGTTATACTTCCCAAAGGACTGTATA1260.10072022957817409No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)