FastQCFastQC Report
Sat 31 Dec 2022
SRR5845280.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845280.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122560
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC3300.26925587467362927No Hit
AACTTAGGGATCTCTGAATATTTTCCTAGAAAATATGCTACCAGCTAAAGCA2750.2243798955613577No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT2430.19827023498694518Illumina PCR Primer Index 5 (95% over 21bp)
GTTGCCGGGGAGTTGCTTCAGCGTCCCGGTGTGGCTGTGCCGTTGGTCCTGT2410.1966383812010444No Hit
ATCAATGGGATTAGAAGACAGTTTGGATGTTTCCTACAAAACTAAACACTTT2360.19255874673629242No Hit
CATACCAGGGGAGGAAGAGAGGAGAGCTGAACTTCGGGCAAACGGCAAAGTC2270.1852154046997389No Hit
TTAATAGGGACAGAAGCTGAAGTTTTCTGGGAGATGTGTAGAATGATGTGTC2220.18113577023498695No Hit
TTGTCTGGGATTCACAAACAATTCCAGAAGAAATAGTTGATATTTTTATGTG2170.177056135770235No Hit
ATGTTAGGGGCTATTCCCAGTTGGAGAGATGAGCAAATATTTCTATGTAAGC2160.1762402088772846No Hit
CATCGCTGGGGTCAACACTGATAAGGTCCAAATAATTTGTATTTGAAACATC2090.17052872062663185No Hit
CGCACTGGGATCAGTGGCCAGTTTTGCATTTCTAGACAAAGAAGCATAGTGC1990.16236945169712794No Hit
GCCTTGGGAGAGGCATGACTCTGTGAATGGCTTCTGGCATTAAATAGCCTGG1980.16155352480417753No Hit
AATTGGGGCTTTTAAGAGGTCTGCCTGGTTCTGGGAAAACAACATTGTCTCG1960.15992167101827678No Hit
GTTTCTGGGAGCCCTGTCCAGCCCGGTTCCCGGGAGGATGAAGTTCGTGTAC1930.15747389033942558No Hit
TTCTTAGGGAACGTGCAAAAATGACCACCAGTCCTATACACCAATAATAGAA1880.15339425587467365No Hit
GCCTTTGGGAACCTCTGTGAACCTCAGTTTACAGATCAGGAAAGGACTATAC1880.15339425587467365No Hit
ATACATGGGAAGAGTAAAACTGCCCTTATAAAAAACAAGACAGAAATCTCAA1880.15339425587467365No Hit
CAATACGGGACATGTATTTATTCAGAAATATGTGCACCCAGACTACTTTCTG1880.15339425587467365No Hit
AAACAAGGGAGGCGCCTGCCACCATGCCTAGCTAATTTTTGTATTTTGAGTA1850.15094647519582244No Hit
CTCGTGGGGACTAAATTTGAGGTGAGGTGTCTTCAGCTTCATGGTGTCCACA1830.1493146214099217No Hit
GTGTCCGGGCGCTCTTCTGATCTCTTGTAGGGGATCTGTTTCATTAAATTTG1830.1493146214099217No Hit
TCAGCTGGGAGACCGGCTGGCCGCGCGCGGGAAGCCGCGGAACTGCGCCGGA1830.1493146214099217No Hit
TTGCAGGAGAGAGAAAAGCTGAAAAAATGCAAAGAATAACCACATACTTTCC1820.14849869451697129No Hit
AACAAAGGGACTGGTCAGACATGCTGGCCAACCCCAGGCGACCTATTGCCCA1820.14849869451697129No Hit
TATCGCGGGATAAAAGTATTGTTTTATGTAGTTACCTACCAATACTATCTGC1800.14686684073107048No Hit
ATCCTCGGGATCAATGTTGTGTTAGAAGATGAAACAAATCAATGAACAATCT1800.14686684073107048No Hit
AATTGCGGGACTTGTACGGACTTTGAAGTGATTCAGTTTCCTCTACGGATGA1780.14523498694516973No Hit
CGCAACGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1780.14523498694516973No Hit
TATATAGGGAGTCTACCCTCCCTTAGCAGGGAACTACTCCCACCCTGGAGCC1770.14441906005221933No Hit
AGAAGTGGCTCTCTAAACTAGAAGCCTTTTATAACTTTCCTTTCATAAAAGG1760.14360313315926893No Hit
CTTGCCGGGGAGGTTGGTCTGGACCGGAAGCGAAGATGGCGACTTCTGGCGC1760.14360313315926893No Hit
TTTGCAGGGTGGCTGGTCGTGGTATTGACATCCAAGATGTGTCTATGGTTGT1760.14360313315926893No Hit
TTTCAGGGAAGAAAAGAAAGGAAAAGTGAAGTAGGCATATGAAAGAAAAGGC1750.14278720626631852No Hit
GCTGTCGGGAATAAAGCCAACTGGAAACGAATCGGAGCCATTTTCATTTTCC1750.14278720626631852No Hit
CGATATGGGGTAAATCAATTTACAACAAACTGAACAAGGCAATTGTGTTTAC1740.14197127937336815No Hit
GAGTAAGGGACTAAAGTAAAACATTATTAGAAATTAGCCTAAAGACTTTGAG1730.14115535248041777No Hit
ACTTTAGGGTATGGGCAAAATAGAAACAGAGAATGAGAAAAAAATAAGAAAA1710.13952349869451697No Hit
GCTCTGGGGTCTACTATGTAGAAGTGACTGAAAAGTCAATCAGAAAATGTGT1700.13870757180156656No Hit
ATCCCCCGTTTTTTGGTCATGTTTCAATTAATTGTGAGGAAGGCGCAGCTCC1700.13870757180156656No Hit
GCTATGGGTGGCAGGACGTCCTGCCCTTCTGGGGAGTTCAAAATGGCTTTCT1690.1378916449086162No Hit
TACAGAGGGGTTGTAGACTGGCTGCTGCTGTACGTGACTGTGATCAAAGTCA1670.13625979112271538No Hit
AACAGTGGGTGAGACAGGGAGGCTATTTTTTAATATAGGATTTAGAAACCAA1670.13625979112271538No Hit
AAACGAGGGATTTGTGTCATGAGCATTTGTTTCAAAGCAGCAATTACATCAT1660.135443864229765No Hit
ACGTCCGGGGACCCCGAGTGTGACTGGGAAGGAGAACTTATTCCTTACCAGG1660.135443864229765No Hit
CTAAGCGGGACCCTGATGCCAAAACCAAACAAAGACAACACAAGAGAAGAAG1640.13381201044386423No Hit
TAAGAAGGGGATCTAAGCATGGGACTTGTACGGACTTTGAAGTGATTCAGTT1630.13299608355091386No Hit
TTGCTGGGATTACAGGTGTGAGTCACTGTGCCCAGCCACCTCTTTTTAATAT1630.13299608355091386No Hit
CCTCCTGGGAGCTATGTTTTGGCATAAGAATAAAGAGTCTCTGTTTTAGAAG1620.13218015665796343No Hit
ATAGTAGGGATAGCTCACTCCAGCCTCCAACTCCTGGGCTCAAGCAATCCTC1620.13218015665796343No Hit
GCATTAGGGATGTATCAATAATATATAACAATTTAAAAATGGGAAAAAGACT1620.13218015665796343No Hit
TAGTTAGGGTCTGCCACTTCCTCTAGTTTGTCCTTAAAAAAGCAAGAGCAAT1610.13136422976501305No Hit
ACTAGGGAGTGAAAAAAATGTATCTTTTTGTTAAAACTGGCTTATTTGAATG1600.13054830287206268No Hit
TCATGCGGGAGAGACTTATGTACAAGGACCTTCAATTTTACTTGAAGTAGTT1600.13054830287206268No Hit
GGCTATGGGGTAAACTTTGCCCTCCTTGAGAAATATGGAACTACCTTAGAGG1600.13054830287206268No Hit
TTATTAGGGTACTTATTTGAAGGTAAAGATTATTCTAAAGAGCCCAGTAAGG1590.12973237597911227No Hit
CGAGGGGATGTCATGAAGGCCAGAGGGTCTCTGTCATTTTTATCCTTCTATG1580.12891644908616187No Hit
GCATTGGGGATTCAGGGAAGTAGTTAAGAGGCTAGGCTTTCAATTCAAACAT1570.12810052219321147No Hit
GTCTAAGGGTCCAAGGCTATAGTGATCAAACCAGCGTGATACTGGTATAAAA1570.12810052219321147No Hit
GCAAAGGGATCCAATTTAAGAATAATACCATACAATAAAGTTCAGAATATAT1560.1272845953002611No Hit
GCACTGGGACCCTGTCAGAAACCAGCACTGGGGGCCCTGCCACCCCCACATG1560.1272845953002611No Hit
AGTCTTGGGCCTGTACTTAGTCACGGGGATGGTGGAAGAGGGAGAAGAGGAA1550.12646866840731072No Hit
TTTTTGGGGGAGTAGAGGAAGGTCGTCTGATCTTTGATAACTTGAAGAAATC1550.12646866840731072No Hit
TGAGTGGGTGCTCTGGAACCTTTTCACAGGAATGAGAATTGAGGGTCTGAGT1550.12646866840731072No Hit
AAGTCGGGGATTTGCCTATCCTAGGTATTTTATTTAAGTGGAGTCATACAGT1540.12565274151436032No Hit
CAATTAGGGCTGACAATGAGTGAAAAGCGGGAATTTCCTACAGTGCCCTTGG1540.12565274151436032No Hit
ATATCAGGGACTAGAGTCATGGTAGTGGGTAAAAGCCAAAGAGCTCAGTTTC1530.12483681462140991No Hit
TCAGGGGGATCTACTCCAGGGGAGCCATCATCTGTTTTGAATGGAGGAAAAT1520.12402088772845953No Hit
GTCGCCGGGCGTGGTGATGCAGGATGAGTCCTTCCCTGCCTGCAAGATTGAG1520.12402088772845953No Hit
AGGCCAGGGACATAGACTTCAAAATAAAGCATAAATAGAAGTGCATGGAAAG1520.12402088772845953No Hit
CTTTCAGGGGGCTCCCCCTCGGAAAGCTGCGAAAGTGCTTTGGCGGTTTGTC1520.12402088772845953No Hit
ATCGACGGGATTCTTTGTTAAAAGGGTGTGTAAGCTCTCAGACCTATCCACT1520.12402088772845953No Hit
GCTTCAGGGAGAACACATATAACAAAATAATAGGCTTAAATCTAACACATCA1510.12320496083550914No Hit
ATGTAGGGGATCTGGTTGGTTTTCAGCCTAGAATTTGATACGCTTTTTAGAA1500.12238903394255875No Hit
GCGGTTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1490.12157310704960836No Hit
TCTTTAGGGGTATTGCTGTGCTCAACAAAGCCAGTTTAGTATACTGTGACTT1490.12157310704960836No Hit
AGCTATGGGTGTGAGGTCTGTGTTGTAAATCATCACCTCTATTCCCCAACTG1480.12075718015665796No Hit
ACAACAGGGAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA1480.12075718015665796No Hit
ATTCGCGGGAATATATTTTCATTGTGTCTTTATAGAAAAATTAAACAATAGA1480.12075718015665796No Hit
GACATTGGGATTCTAATAAAATTGTGTACTATCCTGTGTGCTTAGTTTTAAG1480.12075718015665796No Hit
CAACTAGGGGTTTCTGATGGAAAATGGCGGTTTCAAGTCCCCGATTCCGTGC1480.12075718015665796No Hit
GCTGCGGGGTCCGATCTCTTCTGGGGATTTTTAAATATACACAAGACATTTG1470.11994125326370757No Hit
TGCATGCGGGACCAGAATCTCTGGGACACATTTAAAGCTGTGTGTCGACGGA1470.11994125326370757No Hit
TCGCATGGGATCACCGGGTTCATTACCGGCCAGAGATGGACGGGGAGAAGCA1470.11994125326370757No Hit
TGTGCAGGGGAATGTAATTATACAGTATTTGTCCTTTTGTAACGGGCTCATT1470.11994125326370757No Hit
TGGCGCTGGGAACACCAACAGTATATAAAGGTTCCCCTTTCTCCACATTCTC1470.11994125326370757No Hit
CTCAACCGGGCAGGCAGAGCTGTTCCTCTGCTATTGTTGCTGCGTTTCTTTT1470.11994125326370757No Hit
CAAAGCGGAATCTGGATTACCACAATCCTGACATTCCTGAGTGGAGAAAAGA1470.11994125326370757No Hit
AACAGCCCAGCATCAGGGTCACTGTGGCAACAGCAGGCTCTAGGGGAATCCT1460.11912532637075718No Hit
CGTTGGGGGAATCTGAAGTCTCCTATTTGATCAGGTTATTTCCTGGGACTTG1460.11912532637075718No Hit
CTGACCGGGATCTCAAAACAGTTATAAGATTGACAATATGATCCCTACTTTG1460.11912532637075718No Hit
GTAGGCGGGGCAGCGGGCGATGACGTAGAGGGACGTGCCCTCTATATGAGGT1460.11912532637075718No Hit
CAGTACGGGCTTCCGATCTCGCTGTGGGCTTCAAGACTTTCTTTGCCTCTCT1450.11830939947780679No Hit
CGTTGGGGGAACTGGAACAGTTCTAAAATTTATCTGATACTTCTCTAACAAG1450.11830939947780679No Hit
GTTAAGGGGGTGTAAGAATTAAGAGAAGCCAAAGCTACTGGGAATGAACAAA1450.11830939947780679No Hit
TAACCTGGGAGAATTGCCTTTGATTTCAAATGGTAACAGCATTTATTGGATT1450.11830939947780679No Hit
TAACAGGGATCTCTGACTTAGATACGTAAATTGTTGCAGTTAGAGCACTTTG1450.11830939947780679No Hit
CTCTTTGGGCTCAGCTGGCTAATGGAGAAAATGTGAGGAATTACCTGTCAAA1450.11830939947780679No Hit
ATTGCAGGGAACACCCACTCATGATAGAAACTAGTAAACTAGGAATAGAGGG1440.1174934725848564No Hit
GCTGGTGGGGTTAAATTAATTTTTTTAATTATTATTTAAAGTAAAACTGTCA1440.1174934725848564No Hit
TGAGTGGGGGAACAAGATGGTAATGCAGTTGCCTTTGTTTATTTAAATAGAA1430.116677545691906No Hit
TGAACAGGGAGGGGCCAACTGACACCTCACACGGCCAGGTACTCTTCTGAGA1430.116677545691906No Hit
TTGTCCGGGAATCCATATTGTGGAATGGTTATACTTCCCAAAGGACTGTATA1420.11586161879895561No Hit
CTTTCTGGGGTCATATTGTGAATACTGCTGCAAAGCACGGGACTGCAGACGT1410.11504569190600522No Hit
TCAAGCAGGGGAATGTGAAGAATGGGAAAATATATTTTTGCATGAAAACAGA1400.11422976501305483No Hit
TAAAGAGGGCAAGTTTTTATGTACACTTTGTAAATATCATTCTGTCCTAAAA1400.11422976501305483No Hit
AGCTCGGGACAGTACTACAGGTGCATACTCTGGTCATTTTTCAAGCCATGTT1400.11422976501305483No Hit
AAACAAGGGCACTGGCAGTGAGATCCGCCACCGTGATGATGCTCTACCTTTT1390.11341383812010443No Hit
AACACCGGGGAGGACATTTTATGAGTTTCAGTTATAATTATTCTAGCAAGAA1380.11259791122715404No Hit
GTGATAGGGATTTACTGGAACTGAAAGTTCCAGTTGGGATGAAGCATTTCAT1370.11178198433420365No Hit
TTTTAGGGGTGAAGCAAACCAATTATAATTAACCACATGTTGTAGTAACTGG1360.11096605744125326No Hit
AACCCAGCACAGACCCAGTAGTGATGGTCACAGGGCTGCTTGTGTCACCACT1360.11096605744125326No Hit
ATACCGGGAGAATAGAAAACCCAGAAATAGATCCACACAAATATGCCTAATT1360.11096605744125326No Hit
ATATATGGGAGAGTGCTGAGATTACAGGTGAGAGCCACCATGCCTGGCCTAT1360.11096605744125326No Hit
CATGAGGGGGGGCGAAAGGCATCCCAAGAGGAGACAGTAACGTGTGTATAAA1330.10851827676240208No Hit
CTTCATGGGAGAGGTGATGGGAATGTGTCCCTGTTAACTGACTATATTTATT1330.10851827676240208No Hit
TGTAAAGGGAACATATACATAAACATAGAATAAACATATATATAATTGATTA1330.10851827676240208No Hit
CGTCAAGGGGGAAACATTCGCTTGTAGTTCCATTTTACTTGATCTCTACAAG1330.10851827676240208No Hit
TATATGGGGATTCATGCTGTTATTAGGTTTTCTCCTTCCAGGTGGTTGAAGT1330.10851827676240208No Hit
TGACAGGGGTGATGACTTCTGAGAATCTTCTCAACTTTAGAATTCTCTGACA1320.1077023498694517No Hit
TAGTAGGGGCTCTCCATTGGAAAAGGAGCTGTTGCAAACCGTGTTCATTTAA1310.1068864229765013No Hit
GCGTATGGGGTCCCACACGTGGGGGGTGGTCACCCCCCTTCACTCCAGCCCG1310.1068864229765013No Hit
TAAGTGGGAAGCTATTTCATAATAAATTCTGTACAGTTTCCCCCAAAAAAAG1310.1068864229765013No Hit
ATATACGGGGTGAACTGGATAGGTACCCCACACCTGGATGGGAAGTTACCTT1310.1068864229765013No Hit
TTTCTAGGGATAAGCGTTCATGGAACGCGTTGAAATTGTAATTATATCTGAG1310.1068864229765013No Hit
CGCATAGGGGAAATCTAGTAGACATAACTTTAACCAGGCAATCAAGGTTAAT1300.10607049608355093No Hit
ACAGGTGGGCAGAGCATTCTTCATAACAAAGACCTAACCTCCAGGGGAAAAA1300.10607049608355093No Hit
GATACCGGGGGACAAGTAAAGTTTGAATCAGCTTCTCCATGGCCTGGGCACC1290.10525456919060051No Hit
TCAGTCTGGGATGTTTCCTTGTTAGATTTTTCTCTTCCCACTGGAAGTGAAA1290.10525456919060051No Hit
TCGAGCGGGATGGCAAAAGGGACTTTGCAAATGTATTAAAGTAAGGATATTG1290.10525456919060051No Hit
ATGTTTGGGAGATGTGTCTTTGTTAATTTTCTTTCTAGGTTTTTTGTCCAAT1280.10443864229765012No Hit
TACAGCTGGGACGAGCCACCGCAACTGGCTGAGAATCTTTTTATTTGCTGAT1280.10443864229765012No Hit
AAACTGGGGCAAACAAAGAACAACATGCTATATCTGATTCAATTCTAACATT1280.10443864229765012No Hit
AAAATTGGGGTGTCATTGAGGAAAATTTCTATCATTTCTTGTCAACTACCAC1280.10443864229765012No Hit
AATAATGGGGTGGTGGCTTGCACCTCTGGTCTCAGCTACTTGGGATGCTAAG1280.10443864229765012No Hit
TGCAGTGGGAGCCTCTGTCAGTTACATTTGTAACTTGAATAACATGCTTAAA1280.10443864229765012No Hit
ATGTCTGGGAGCTTCAAAATGATATTGAAATCCACGTATCCTATATTTTGAA1270.10362271540469974No Hit
GTCCGTGGGGGTTCAAATTTAACATGAGAGATTAAGAAATATATGTGATACA1270.10362271540469974No Hit
CGTTAAGGGATTCAAGATGGCACTGACACGGGAAGGCCAGCTACAGGTGGAC1270.10362271540469974No Hit
TTCTGTGGGGTTACATATTTTGAGAATCAAATTATTTTATAAAAGATATAAT1260.10280678851174935No Hit
AAGTCGGGGGATCTAATGGTGGGGTTTGGAACTCTGAAGGATTTAGACAAGG1260.10280678851174935No Hit
TGTTCAGGGACAACCTGTATATTGTATGCATTGCTGCGTGGAGGCTGTTTAT1260.10280678851174935No Hit
AAGCCTGGGCGTGGTGGCAAGTCTAAAGATGAAAAATGTGTCTGCATACCAA1260.10280678851174935No Hit
GCGAAGGGGGGCAATTAATTGGATATATGAGTCTGGAACTCTGGGAAAGTTT1250.10199086161879897No Hit
TATCTAGGGGTGTTTTCTCTGGCACAGAGTAGATCTTTTGGGAAATATATAT1250.10199086161879897No Hit
GTGGTAGGGATATAAGTACTAAGTTCTGAATGTGAATGTGATCTTACATAGT1250.10199086161879897No Hit
GTACTTGGGGCAAGAAATTGCCCGGAGTTTTGGAAAACTGATGAGTAAAGGC1250.10199086161879897No Hit
TTTTTGGGGACGAAAGTCTTTTTTTAATGACATTTTCTTCTTGAAGACAGCA1250.10199086161879897No Hit
TCTGCTAGGGGGAGCCTGAGCTTGACCTCAAACGGGTGGCGATTTGATGGGT1240.10117493472584856No Hit
ATTCTAGGGATTGGTCATGATGTGGAGAAATTGAAATCATACTTGGTGGAAA1240.10117493472584856No Hit
GTTCTAGGGTACCAGTTTGGTTTTAGTTTGTCTTTGTCAGCGCCACGTGAGA1240.10117493472584856No Hit
AGCCAAGGGTCCGATCTGCCGGGGATTTTATTTTATTCATGAGCTGAGATGA1240.10117493472584856No Hit
CTTCATGGGGTGGATTTTGTCCATTCATCTCATGCTCTCTGAACAGAAGGTA1230.10035900783289817No Hit
CGCAAAGGGAGGTTAATAGAGGGTGGTATTTAGGAGATGTTGAAAGGCTGTG1230.10035900783289817No Hit
TTAAATGGGATCCATAAGAATGTTTACACATGGCAGTATCAAAGCATATAAA1230.10035900783289817No Hit
TTCTCGCAGGGACTGTGCAATAACTGATTCATTTTTTTCAGAGCTTGAAGCA1230.10035900783289817No Hit
AGCCTGGGTGACAGAGCGAGACTCCCTCTTAATAAAAAATAAAATAAAATAA1230.10035900783289817No Hit
CATTTGGGGACTCAGCACTCCTGGAAGTGTCTGGATACAGCTCACTGAGAGT1230.10035900783289817No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)