FastQCFastQC Report
Sat 31 Dec 2022
SRR5845279.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845279.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33201
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT4571.3764645643203517Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC3931.1836992861660793No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCTTATAC790.23794464022168005No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTG740.22288485286587756No Hit
GCACGCGGGCCTCCCTAATTTCCACTCCCCCCACCCCACTTCGCCTGCCGCG460.13855004367338333No Hit
ACTGGCGGGTGCTTCACCCACTGCTCCTGCCCATGTGGGGTTTGTGGTGGTC430.1295141712599018No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCT420.1265022137887413No Hit
ATTATGGGGAAAGAATAAATGCGTGCATTCCATAGGATGCAAGAAAAGGAGT420.1265022137887413No Hit
ATGCCAGGGCATTGATGAGGAACTAGAACGGGACAAGAGGGTCACTTGGATT420.1265022137887413No Hit
TAGAAGGGGTGTTGGTGGAGGTGCACCTGACTGCTCTGTGAGAATCCGTGCC420.1265022137887413No Hit
ACATTTGGGACGAACATTAGAACCACAGTTTTGGTTCAGGTAAGATCCCAAA400.12047829884642028No Hit
GCACAGGGGGGGTAATAAAGAAAAAAGAAAAAAATTGCACTCCCTACCACCT400.12047829884642028No Hit
AAGCCTGGGACAGTGGACCTTTAGAAGTTTAGCTCGAAACCTTAAAAGGCCT390.11746634137525978No Hit
ACCACTGGTTGTTGAATGGATGAATGCTTAAATCCTTCCAGGTATATTTGGC380.11445438390409927No Hit
TTGTTGGGGAGAGTCAATAATAGAAAGGTATTTAGAAAACCCACAAACAGCT380.11445438390409927No Hit
GACTCTGGGGTGTCTCGGTTGGCCGTGAAGGCCGCCTTCTTCGGCCTGCTGG370.11144242643293878No Hit
TACGCAGGGAGCTTCTAACACGTGAACTTTTGGGGGATACATTCAAACCATA370.11144242643293878No Hit
CATAATGGGTCTAATCCATATAATGAACAAATATGTTAAAGTGAGACAGTCA370.11144242643293878No Hit
GTATAGGGGATTTCTTCAGCTACTGATTTTTAGAACAACACAGGGCTGCGGA370.11144242643293878No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGA360.10843046896177824No Hit
CTTCGAGGGAACTTAAGAGTTGGCACGGAAACATTATTGCTCAAAAGAGCCA360.10843046896177824No Hit
ACTCCTGGGGATAATCGGGGGAGGTGTAGAAAGGCCTCCGTGAATGTTGGCT360.10843046896177824No Hit
TTCGTAGGGATGGAATTATGCTGGAAGTCTCAAATAATATTTTTTTCCTATT360.10843046896177824No Hit
GCCAAGGGTCGCATAAACAGGAGAATGAATTGAAAATTGTAATTAAGATTTA360.10843046896177824No Hit
TGCAATGGGGACTTTTGACAAAGGACTGCGGGCCTCGCTCAAGTCCCTGAGC350.10541851149061775No Hit
TGCGCGGTGGGTATCACCTGATTTCCGTCATGATACTTGGGCGTGTTATTAT350.10541851149061775No Hit
CAAGCAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA350.10541851149061775No Hit
AATAGCGGGGCCAAGAGGAGGATCCATCAGTCAGTTGAAAGGCTTAGAATTT350.10541851149061775No Hit
TTAAAAGGGTCAGGAACTGTGGGACAAACTTGGATTCATCTGAAGCAGAGGG350.10541851149061775No Hit
TTTCTGGGGTCTTGAGAGAAGCCTGCTGTTCCTCCCATCATTTCACCTGCCC350.10541851149061775No Hit
TCTCCTGGGCTAGGATGGAAGTGCCCTGTGAGCTGGGGGGCCCTTCAAAGGG350.10541851149061775No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGT350.10541851149061775No Hit
GGAAAAGGGGTTTAAGTGAAATGATGTACATATTTAATAATTTTGGCCTTCT350.10541851149061775No Hit
CTTAAAGGGGCAGAAACTATAAAACTCCTAGAAGATAACACAGAGGAAAACT350.10541851149061775No Hit
CATGTAGGGCATGTTATTTCCTCAGGTCTAGAGCTATTTGATTTCCAAAATT350.10541851149061775No Hit
CTGTGCGGGCAAGAAAAAACAGTGACAGAAACTTAATTTTCATAAGATTGCT340.10240655401945725No Hit
TGGTCTGGGATTCCTAATGCTCAGTACAACTCCACACAACTTGAGACCTGAA340.10240655401945725No Hit
AATTTTGGGCTTGGCTCACTGCAACCTCCACCTCCCAGTTCAAGTGATTCCC340.10240655401945725No Hit
TGAAAAGGGGTCAATAAAGTGGCTTGTACCTTCAAATAATTTTTTGAATTTT340.10240655401945725No Hit
AATATGGGGACTCCTGCTTTCTTTTGATTAATGCTTGCATTACATATCTTTT340.10240655401945725No Hit
AACGTCGGGAACTACAATATTGTTTATCTTAGTTTCATAGTAACCACAAAGA340.10240655401945725No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)