FastQCFastQC Report
Sat 31 Dec 2022
SRR5845278.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845278.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32872
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT4171.268556826478462Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC3521.070820150888294No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTG590.17948405938184472No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCTTATAC570.17339985397907032No Hit
TGCACGGGACAGACACAGAACCAGATTAAGTATAATTCAGGATCCTATTGTG520.15818934047213434No Hit
CACAAGGGGCCATTCAGAAAAAATGATTTGGAGCTGTAGGATTCAATGACTG430.13081041615964956No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCT430.13081041615964956No Hit
GCCAAGGGTCGCATAAACAGGAGAATGAATTGAAAATTGTAATTAAGATTTA430.13081041615964956No Hit
CTACTTGGGGTGAGGAATGGGAAAATTGGAAGAACGAGAGGGGGAGAGATGT400.12168410805548796No Hit
AGCAATGGGGAAATTTGTCATCCTTGAATTCATCCAGATCGATCTTGAAACA400.12168410805548796No Hit
GACTAGGGGATGAAATGCAGTTCTACCAAAACAATAAAGTTGTTTAGTGGAG390.11864200535410074No Hit
TATAATGGGGTCCGCCCACCTCGGCCTCCCAAGGTGCTGAGATTGCAGGCGT380.11559990265271355No Hit
TAAGCCGGGTGCTTTCTCAATCTTACCAATCAGCCACCACTTTAACCAGCCC380.11559990265271355No Hit
TTTCAGGGGTTCAGAGTTAGTGCTTCCTATTCAATAAATAATCATCAAAATT380.11559990265271355No Hit
CCTAGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC370.11255779995132636No Hit
ATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCC370.11255779995132636No Hit
CCAAGCGGGTGCCCACCTTCCAAAAAGTTCCCCTGCCCTCGGGCCCTGCACC370.11255779995132636No Hit
GTTGCGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC370.11255779995132636No Hit
TTTCTAGGGTTATGTGCTGGGGCAGAAGAGATCCGATAGCTATGTTCTGCTG360.10951569724993918No Hit
GCCATTGGGGACAAGCGTGAGCTACCACACCCAGCCCGGTCTTTTTTAGACT360.10951569724993918No Hit
ACCACTGGTTGTTGAATGGATGAATGCTTAAATCCTTCCAGGTATATTTGGC360.10951569724993918No Hit
GGCGAGGGGTACAATTAATAATCAGGATTAGAACACCTTGCCTTTGTGTTGG360.10951569724993918No Hit
CTTACTGGGAGATCTGATCGAGTCAGTATGTCTTCAGCTTGATCAGATTTTA360.10951569724993918No Hit
ACACTCGGGGAGCGAGTTGCTGACGCTGCCCGTGCTCATGAAGCGCTCCATC350.10647359454855196No Hit
CACGAAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG350.10647359454855196No Hit
GCGCTGGGTAATTTTACTAATGCTAATCTCTCCCACTCATCTCTTAATTACA350.10647359454855196No Hit
CTAGTTGGGGTGACAGAGCGAGTCCCCACGGCTCTGGGAGTGCCTCGCATGT350.10647359454855196No Hit
CATGTAGGGCATGTTATTTCCTCAGGTCTAGAGCTATTTGATTTCCAAAATT350.10647359454855196No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGT340.10343149184716477No Hit
TGCAATGGGGACTTTTGACAAAGGACTGCGGGCCTCGCTCAAGTCCCTGAGC340.10343149184716477No Hit
CTGACAGAGGGTCTTACCTTTAAAGATATAGTTTACTATTGATAATGATTTA340.10343149184716477No Hit
ATATGTGGGATAATTATTCTTATACCCTAAAAATTTAGATTTGTCTCTAAAG340.10343149184716477No Hit
CAAGAGGGGTCTCAAGGCGGGGTGATTGAACATCCTGAATACGGAGAGGTTA340.10343149184716477No Hit
GCGCATGGGATCTTTTTCAAGGACCTGGGAGCCATCCCTTTGAAATGTCATC330.10038938914577757No Hit
GTGGGAGGGGAAGATTTCTGCTTTGATGGACAAATTCGAGCACCAGTTTGAG330.10038938914577757No Hit
CTTAGGACGGGATGATGCAGTATATAAGTGGTGAACAATAACTGACAGTATT330.10038938914577757No Hit
TGAAAAGGGGTCAATAAAGTGGCTTGTACCTTCAAATAATTTTTTGAATTTT330.10038938914577757No Hit
GATTTGGGGATAACTTTGATAAGTGGTTGGAACAGGTGGGAGAAAGCAGAGG330.10038938914577757No Hit
CTAAAGGGACTCAATGCAATTCTCAAGTCTATAAGAGGTATGTGCTTAATAT330.10038938914577757No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)