FastQCFastQC Report
Sat 31 Dec 2022
SRR5845276.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845276.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35858
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC2890.8059568297172179No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT2760.7697027162697305Illumina PCR Primer Index 5 (95% over 21bp)
ATACTGGGGGATTTCTCTATCAGCACTTCCTTCTGCCTCCCAACTACTTGCC610.17011545540744044No Hit
GAGTTGGGGGAATTAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGT590.1645378994924424No Hit
CACCTTGGGACATATTTATTTTTCCTTTTTTCTTTCTATTCTATCATTATAC560.15617156561994536No Hit
CATACTGGGATAAATTAACATAAGTGTACTGTAACTTTACAAACGTTTTAAT530.14780523174744828No Hit
CCTGAGGGGAATCATGTCTAACCAAGTAATAATGTTTTAGCAATAAAATTAC510.14222767583245022No Hit
TATGCAGGGGTGAATGACAGCAGCTGGCACTTCCTCATGGTGAGCCGTGACC510.14222767583245022No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCTTATAC500.13943889787495117No Hit
TTTGGTGGGGTAAATATGGCTTAGCCTAATAAGAAGCTATTTTGAGAACTTT490.13665011991745218No Hit
ATCCGGGATATACTAATAACTTAGTAGCTCTTTGAAGAAATAATACAGATAT490.13665011991745218No Hit
TTGTGGGGTATATGTAGGTATATTATCCATACTTTAGAATTTCATAATCCAC480.13386134195995317No Hit
CTATATGGGACGTTGATGTAACCGAATTTACATTGCTGAATATGTTGGTGAT480.13386134195995317No Hit
TACTTAGGGAGTCTATTAAAGGTGTTTGTTTAAAATGGATATAGTTTTAGAT480.13386134195995317No Hit
ATCAAAGGGCTTTTCTAATATTTTCAGAAAGTGATGGGATAACCCTAGAAGA480.13386134195995317No Hit
GCACTTGGGGACAAAGAGGCATCTGGTCTTAACTGCTTGCTTCTAGTGGCCA470.13107256400245412No Hit
GTTTACGGGTATGATGCCTAGAATTACATTTTGTTGCCTGTTTGAATCTCTC460.1282837860449551No Hit
CTTGATGGGATTCCTGCACTGGTTGCCACCAACATTCTCTTGGTTGATGAGA460.1282837860449551No Hit
TCATAGGGGCGACTGTGTCATGACAAGGAAACTTACAAACTGCAACGCAGAG460.1282837860449551No Hit
TGTTCGGGGGATGCGCCTTCGGCGATTTCTCTGAGAAACGGTTGCGTTGACA460.1282837860449551No Hit
TACACAGGGGTATGGAGGGTAATCTCTCTTTCTGTCCCCAATGTGCTTTTCT460.1282837860449551No Hit
TGCAAAGGGCTAGCAACATACCAGGTAACAAAATAATGTTTGTTGCAATGAA460.1282837860449551No Hit
TTTCACGGGTGCCCCTTCAAATGTTTCATCTAAAGGTGACAGCTTGTGGGCA450.1254950080874561No Hit
GTTTAAGGGAAGTATGAAAAATAGAGTGAGACCTAGGAGAATAAAATCTTTA450.1254950080874561No Hit
GCACGTGGGCGCTCTTCCGATCTAGCGTGGGGATCCTCTAAAATTACCCTTT440.12270623012995704No Hit
TGCTTAGGGATTGGAACAAGTGTATTTTGTCTTAGACTAATGGTGATAACAG440.12270623012995704No Hit
GCTCATGGGCAAACAGGTTCTATTACATGAGGAGCCAAGGCTTGAAAGGAGA440.12270623012995704No Hit
AACCCAGGGGTCAAATGATAGTCTCCAGATAAAGAAATGCTCTATAATGCCT440.12270623012995704No Hit
AAGCAGGGGGCCCCGCCCGCTCCGGAGCAACCCGCGAGCTTACACCGGCTTC440.12270623012995704No Hit
AGCACTGGGAACTCAAAGACACAGAAAACTAGAGAAGAGAAACATCAGAAGG440.12270623012995704No Hit
ACGAACGGGAAATGATGTCAAGGTAGCAACAAAACCCAAAAAATCCATCCTT440.12270623012995704No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTG440.12270623012995704No Hit
GTTAATGGGCCGATCTGAACCAGGACACAAATTACTTATTAACATATGAAAA430.11991745217245803No Hit
GTAACCCAAACCTCAGCATCGCACAATATACCCATGCAGCAAACCTGCACAT430.11991745217245803No Hit
TCATCAGGGTACATATGCAATTAGAAATACAGAAAAAGGAATCTACTACCCG430.11991745217245803No Hit
GCCAAGGGAGAACACATCCCAGAACAATTACTGAAAAGCTGAAATTTAATGG430.11991745217245803No Hit
ATTGCCGGGAGTGAGCAGTGTTGCGTTTTTCCTTGTAGCATTTGGAAATGAT430.11991745217245803No Hit
TCAATCGGGGTAGCTTTTTTGACCATTATTTTTCCTTCATATCACTATATTA430.11991745217245803No Hit
ACTTTCGGGGTGACGATGGATTCCTCGCTGATGACAACTGCAGTTCAGAAAT430.11991745217245803No Hit
TAGCAACGGGGCCAAGAACTGATTTATTTATTTTTTTTTCTGAGATGACATT430.11991745217245803No Hit
GATGCAGGGAAATTAATGTGAGGAAGCTCCTCCAATCCTCTGGCTATTTAAG420.117128674214959No Hit
GTCTAGGGGTCAGTCGGGTGTTGAAGTCTCCCACTATTATTGTGTGTAAATA420.117128674214959No Hit
TGTTAAGGGTGGAGTTCTGGCCTCCAGAATGTGAGAGAGTAAACTATTGTTT420.117128674214959No Hit
CCACCAACTTAAGAAAACTGGCTTAGGAAGGTTAAACATGAGAATAGGCCTC420.117128674214959No Hit
ATCGATGGAAAAGGAATGTTCTGTGGAAAAGGGAACTGGTGATTCACTAAGA420.117128674214959No Hit
TCTATTGGGGGTGTGCGCAACCGCACCCAGCCTCAAATGACTTTAAAGTTTG410.11433989625745998No Hit
CCAAGCGGGGTACATGGTTGGCAGAGGATCTTGCAGCTCATAGTGATAAACT410.11433989625745998No Hit
CGCTCCGGGGCCAGGTGGACAAGTGGCAAGAGGAATACAATGGCGGGGAAGG410.11433989625745998No Hit
TAAAGCGGGATCCTCTCTCACCATTTCTATTCAACATTTTACTGGAAGGCAG410.11433989625745998No Hit
TGGGGCGGGAGTATTGCAGTTATACATGGGGGTTTTGGTACTTTATATTGTG410.11433989625745998No Hit
TCACAGGGGGATCTTGAAACGGGAAGAATAAAATAAAACTTAAAAAAAGAAT410.11433989625745998No Hit
TGCAATGGGTATTTCCTCGGATGCAGTGTAGCAAGTAGAAAGTGAAATACAG410.11433989625745998No Hit
GTTAAGGGGCATTATTCATTTTATGAAGACAATTTTCTTCTAAAGCTAGATC410.11433989625745998No Hit
GATAATGGGTCTGGTAGCTCAGTTAATAAAACAATTTCAGAATTAAAGAAAT410.11433989625745998No Hit
GTGGCTGGGGAATTAACAACTGGGACAGGGGACCATCACTCATAAAATTTAA400.11155111829996096No Hit
AACAGTGGGATAAATATTCTTGCACAAGTCTCTTTGTGAATACATTTTTATT400.11155111829996096No Hit
ATTAAGGGGCCATGGGTCCCGGGCGGCCTTGTGGCTCTGGTAATGTTTGGTA400.11155111829996096No Hit
AGACCAGGGTCGCCTGCTTTTGACCCAAAGGTCCTGACGGTTGCGTCCCGGG400.11155111829996096No Hit
CGATAGGGGACTTGTACGGACTTTGAAGTGATTCAGTTTCCTCTACGGATGA400.11155111829996096No Hit
AATAGCGGGTGCCATCATATGATCATTTATTTACTTTTTGTGCAAGCAATGG400.11155111829996096No Hit
TTACAAGGGGTGCACAAACTAGAAAACCTAGAAGACATGGATAAATTCCTGG390.10876234034246192No Hit
TGTTTATGGGGAAAGCTTGAAAGTAAACAGATTAGCAAATATGTATGTGTTA390.10876234034246192No Hit
GATTGTGGGGAAATGATATTTATATTCTCTATAGGGGACAGAAAATGAGAAG390.10876234034246192No Hit
TCGCCTGGGAGTTCCACCTAGTGGCACACTCTTTCCTTTCCTCCTATCTCAT390.10876234034246192No Hit
ATGCAGGGGCAAAGTTACCAGAAGAAGAAGGACTGGGAGAGAAAACCAAGCT380.10597356238496292No Hit
AGTAAAGGGCTAGTAGAGAGGAACCGCATCGGCTCAGAAAACCCAAGCGATG380.10597356238496292No Hit
ACTCTGGGGACTTGCAGGGTGTTTAATCGTCGCCAAGCGGGACTTACTGCAA380.10597356238496292No Hit
AGTACTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA380.10597356238496292No Hit
AAGACTGGGATAAAATTTTCATTTGAGAATTTCTCAATTGTATCTAGTTAAA380.10597356238496292No Hit
TTTAATGGGACCCTTGCCCTAAAGCAGGAGTCCCCCCTACCTGGGGTCCATG380.10597356238496292No Hit
GTTTATGGGCTGCTAAACTTGTTGAAATGTTTCTTTGGACAGATTAAGTTGA370.1031847844274639No Hit
AACTAAGGGTATAACAAACCCTGAGAACCAAAATGAACGAAAATCTGTTCGC370.1031847844274639No Hit
GACAACCGGGATACAAGTAGAAGTGGAATTGTAGATTTATTAGATTTTTCCA370.1031847844274639No Hit
TGTAGCGGCAAGCCAAGCATCAATAATTAAGTTATCCTGAAATATTGTAAAT370.1031847844274639No Hit
CACAATGGGTGGGTCCTCTTCACACCTGATAGAAAAAATAAAATTCCTCACC370.1031847844274639No Hit
AGGGAAGGGGACCGATAAGGATTCTTTTAGCTATTTTCATGAAAAAGTGGAA370.1031847844274639No Hit
CATACCGGGGGGTGTCAGGAATATTTGTAGATGAAGTAATTTGTGGCATGAA370.1031847844274639No Hit
CTCTCGGGGTAGAACCATTTGGAAAAATGAAATTTAGTAGTTCCAAGTTTCA370.1031847844274639No Hit
ACTTGCGGAAATGCAAGTTGGAGGAGTGCTTCTGGTATTTCAGAATTACGAA360.10039600646996485No Hit
ACGCCCGGGTTAATTTTTGTATTTTCTTAGAGATGGAGTTTCACTATGTTGG360.10039600646996485No Hit
CAATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA360.10039600646996485No Hit
TAAGCAGGGATAGTTTTCAAACTAACAAGCCTGCGATCCTTGTTAGTGTAGT360.10039600646996485No Hit
CACGACGGGTCTTCAAAGGCACTTGGCATGTTGTTCTGGCATAAACATAACA360.10039600646996485No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGA360.10039600646996485No Hit
CAATACGGGAACCTGAGAATGGCTGGATCATATGGGTAGATATATGTGTAAC360.10039600646996485No Hit
AACATGGGACCAGGCGCGGCGGTACCTCACGGTGGTGAAGGTCACAGGGTTG360.10039600646996485No Hit
GCTTTGGGGAAGTGGGTGGAGAATAACACAAACAATCTGGTGAAAGATTTGG360.10039600646996485No Hit
TTTGTTGGGTATTTAAAAATCCAGATTTTACTTACTTACCCACTAGGGGAAT360.10039600646996485No Hit
ACAAACGGGAGACTAGTAGCAAGTGTAAATAAACACTTCAAATTTCTATTCT360.10039600646996485No Hit
AAGCAAGGGATGTTTTTCTGATCCATTATACTCTTTCTTTAAAAAGGTAATA360.10039600646996485No Hit
GCTTTGGGGAGAAGAAGGAGAGCGAGCGAGCGAGCAAGGGAGCGAGCAGGAC360.10039600646996485No Hit
TTCTACGGGGGCCTCAGTTCATGCATGGCCATCCTGAGATTTGAGAGCCCAG360.10039600646996485No Hit
AGTTGCGGGATCTTCAGCAGAGATGGGAGTTCAGAGTGTCATTGTTAAATAC360.10039600646996485No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)