FastQCFastQC Report
Sat 31 Dec 2022
SRR5845275.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845275.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36252
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT2890.7971973960057376Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC2340.6454816285998014No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTG720.19860973187686196No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCTTATAC640.17654198389054399No Hit
TACCTCGGGAGTTTTGAAATGCAACTTAAGCCATTAATTGTTGGTGTGAATT640.17654198389054399No Hit
ACGCCCGGGTTAATTTTTGTATTTTCTTAGAGATGGAGTTTCACTATGTTGG590.16274964139909523No Hit
TATAGTGGGGTAGACAGCTAGATGATTCAGAGTCTGTTCACTTGAGGATCTT570.15723270440251574No Hit
TCGCCTGGGAGTTCCACCTAGTGGCACACTCTTTCCTTTCCTCCTATCTCAT540.14895729890764647No Hit
CACAATGGGTGGGTCCTCTTCACACCTGATAGAAAAAATAAAATTCCTCACC520.14344036191106696No Hit
TAAAGCGGGATCCTCTCTCACCATTTCTATTCAACATTTTACTGGAAGGCAG510.14068189341277723No Hit
TGTTAAGGGTGGAGTTCTGGCCTCCAGAATGTGAGAGAGTAAACTATTGTTT500.13792342491448747No Hit
GGTTATGGGGTCTAATGTGTCCTATAAAAACCCATCTCTGATGCCTAAGGAA490.13516495641619772No Hit
GATAGGGGCACACCACGTCTTTTCAAAAACTAAAGAGAATTCAAAAAGGGCT480.132406487917908No Hit
GTTCCTGGGGGAGCACTACCGGAAAGCTGGCTCTGTAGAGCTCCCAGCGCCT470.12964801941961823No Hit
GTTTAAGGGAAGTATGAAAAATAGAGTGAGACCTAGGAGAATAAAATCTTTA470.12964801941961823No Hit
CACCTTGGGACATATTTATTTTTCCTTTTTTCTTTCTATTCTATCATTATAC460.12688955092132848No Hit
CCATTGTGAAGTAAGAGATTAAGGCAAGTCAATATTCCTGACCACCTTCAAG460.12688955092132848No Hit
GCTTTTGGGCAGTTTTCCCAACACCATTTATTGAAGACCCTGTCCTTTTATA460.12688955092132848No Hit
AGCACTGGGAACTCAAAGACACAGAAAACTAGAGAAGAGAAACATCAGAAGG460.12688955092132848No Hit
TCAATCGGGGTAGCTTTTTTGACCATTATTTTTCCTTCATATCACTATATTA460.12688955092132848No Hit
ATATGAGGGATAATTTATATTTCTTGTCAGAGTGCTCCCAACTTGCTGTAAA460.12688955092132848No Hit
GCTGTGGGTGCCCACCTCAAGTTTAATAAATAAGGTTGTACTTTTCTTACTA450.12413108242303873No Hit
ACATGGGAGCGTTTTGCCAATTAAGCTCTTTGAGACTCAGCTCTTAAATTCA450.12413108242303873No Hit
TCATAGGGGCGACTGTGTCATGACAAGGAAACTTACAAACTGCAACGCAGAG450.12413108242303873No Hit
CTATATGGGACGTTGATGTAACCGAATTTACATTGCTGAATATGTTGGTGAT450.12413108242303873No Hit
TACACAGGGGTATGGAGGGTAATCTCTCTTTCTGTCCCCAATGTGCTTTTCT450.12413108242303873No Hit
GTTAAGGGGCATTATTCATTTTATGAAGACAATTTTCTTCTAAAGCTAGATC450.12413108242303873No Hit
GTATACGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC440.12137261392474899No Hit
GTTAATGGGCCGATCTGAACCAGGACACAAATTACTTATTAACATATGAAAA440.12137261392474899No Hit
GGTGTAGGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCT440.12137261392474899No Hit
GAGTTGGGGGAATTAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGT440.12137261392474899No Hit
TGACTGGGGGCTATAGCCTATGGGCAAAGAGGCAGTTCTTCTGGGTCTTGAC440.12137261392474899No Hit
GTTCCAGGGTAGACTCAAACAAGTAAAAGAAAGAACATAAGAGCTGAAAGAC440.12137261392474899No Hit
TCATCAGGGTACATATGCAATTAGAAATACAGAAAAAGGAATCTACTACCCG440.12137261392474899No Hit
AACATGGGACCAGGCGCGGCGGTACCTCACGGTGGTGAAGGTCACAGGGTTG440.12137261392474899No Hit
TGTTCGGGGGATGCGCCTTCGGCGATTTCTCTGAGAAACGGTTGCGTTGACA440.12137261392474899No Hit
ATTGCCGGGAGTGAGCAGTGTTGCGTTTTTCCTTGTAGCATTTGGAAATGAT440.12137261392474899No Hit
TTTACAGGAACATTAAACTTAGGTCCCTGTAGGAGTCCATTCAAGAGCAGTG440.12137261392474899No Hit
TACATGGGGAAGAGAGCTGGTCAGTTGTAAAGTACCATCAGAATTGATTTCC440.12137261392474899No Hit
ATATCCCTGGTGAATATAGATGCAAAAAACATCAACAAAATACTAGCTAACC440.12137261392474899No Hit
TACATTGGGGCCGTTGGGGCCAAATAATGTTAACTGCATTGTTCTAGTTGCT430.11861414542645922No Hit
TCACAGGGGGATCTTGAAACGGGAAGAATAAAATAAAACTTAAAAAAAGAAT430.11861414542645922No Hit
CATACTGGGATAAATTAACATAAGTGTACTGTAACTTTACAAACGTTTTAAT430.11861414542645922No Hit
TTATATGGGGGGGTTGCAATCCTAGTTTCTGACAAAACAGACCTCAAACCAA430.11861414542645922No Hit
GCACTTGGGGACAAAGAGGCATCTGGTCTTAACTGCTTGCTTCTAGTGGCCA430.11861414542645922No Hit
CCTGAGGGGAATCATGTCTAACCAAGTAATAATGTTTTAGCAATAAAATTAC420.11585567692816948No Hit
TTGGCTGGGGCTCTTCCGATCTTCAAATTGGGAGTACTGAAATCAAAGGAGC420.11585567692816948No Hit
GTTTACGGGTATGATGCCTAGAATTACATTTTGTTGCCTGTTTGAATCTCTC420.11585567692816948No Hit
ATGCGTGGGAACCTTTATGATCATTGCTTAGAGGAGATACACATTAAGGAAC420.11585567692816948No Hit
AGGACCGGGCCTTACAGCCCTGCTGCTGGGGTTGATTTTGAACTGGAGTCAT420.11585567692816948No Hit
AGACCAGGGTCGCCTGCTTTTGACCCAAAGGTCCTGACGGTTGCGTCCCGGG420.11585567692816948No Hit
AAATCGCGGGAAACAAACAACAGAAACTATAAGAGCAACCAGATGTCAGACT420.11585567692816948No Hit
GTAACCCAAACCTCAGCATCGCACAATATACCCATGCAGCAAACCTGCACAT410.11309720842987973No Hit
ATACTGGGGGATTTCTCTATCAGCACTTCCTTCTGCCTCCCAACTACTTGCC410.11309720842987973No Hit
CGTCTAGGGCTTAGATTCAGTGAGTGAACAATACATTTTTATTTTTTATTAT410.11309720842987973No Hit
TGCAATGGGTATTTCCTCGGATGCAGTGTAGCAAGTAGAAAGTGAAATACAG410.11309720842987973No Hit
AATTATGGGAAATCAGTAAGACTAAAACAAAGGGCCATTTCCCATAAGCATA410.11309720842987973No Hit
AATAGCGGGTGCCATCATATGATCATTTATTTACTTTTTGTGCAAGCAATGG410.11309720842987973No Hit
ACCAATGGGATTTTGCTGATACTGTATATATGCGCATCATTCCTGATTAGAT400.11033873993158998No Hit
GTCTAGGGGTCAGTCGGGTGTTGAAGTCTCCCACTATTATTGTGTGTAAATA400.11033873993158998No Hit
TGTCCTGGGCGCTCTTCCGATCTATGCGCTCGGGCAGTTCTTTTAAGCATGA400.11033873993158998No Hit
CAATACGGGAACCTGAGAATGGCTGGATCATATGGGTAGATATATGTGTAAC400.11033873993158998No Hit
GACCTAGGGGAGGAGGCAAAGGCAGAGGCAGAAGAGGGTGAGCAAACAAGGG400.11033873993158998No Hit
AGGTGTGGGATTAAGAAGAATTTATGTCGAAACTTCATGCTTCATCTAGTCA400.11033873993158998No Hit
ACGCGGGGTGAAAGACAGAAGTTTATTTAATGTAGAGGTAAATGAAGGTGAG400.11033873993158998No Hit
AAATGGGGGAAGTTACTGGCTGAGTGAGGGCATAGAAGTTAAAGGTTACTGT400.11033873993158998No Hit
CACACTGGGATCTTTGGGGAGTTTCAATTAACAAATTTCTCAACTAATATTT400.11033873993158998No Hit
CCTTGGGGAGAACATTCTCAGATCTTTTCAGTCAAAAATCTAAGATGATTTA400.11033873993158998No Hit
ATCCGGGATATACTAATAACTTAGTAGCTCTTTGAAGAAATAATACAGATAT400.11033873993158998No Hit
TTAGAGGGATGAAATCTCCAGCCATCACAGCCACCCTAGAGGAAAAAAATAG390.10758027143330023No Hit
GTTTATGGGCTGCTAAACTTGTTGAAATGTTTCTTTGGACAGATTAAGTTGA390.10758027143330023No Hit
CACACAGGGATACGGGAATCGCGGAGCTTAGCTGTCGCCACCTCGCGCCGGG390.10758027143330023No Hit
AACAGTGGGATAAATATTCTTGCACAAGTCTCTTTGTGAATACATTTTTATT390.10758027143330023No Hit
AAGCAGGGGGCCCCGCCCGCTCCGGAGCAACCCGCGAGCTTACACCGGCTTC390.10758027143330023No Hit
TAGCAGGGGATGGTGGGAGGAGCCGATACTGAGCTTCTTCCTATTTGCCATG390.10758027143330023No Hit
GATAACGGGATACCTCCCAGTCTGGAGATGAGATGACATCTCTGTCAGAGTA390.10758027143330023No Hit
ACTGAAGGGGATAATTGTGGTATAGTGACCAATAGATGCCAAACAGCTTGTG390.10758027143330023No Hit
TGCAAAGGGCTAGCAACATACCAGGTAACAAAATAATGTTTGTTGCAATGAA390.10758027143330023No Hit
ATGCAGGGGCAAAGTTACCAGAAGAAGAAGGACTGGGAGAGAAAACCAAGCT380.10482180293501049No Hit
TCGTCTGGGGTATAAAAAAAGGGGTTTTAAAATGATGCCTACTTTTAATTTT380.10482180293501049No Hit
GGCATGGGGGCTTCCCACTCGGGTTTGACCTACAGCCGCCCGGGAGAAGATG380.10482180293501049No Hit
ATAGTAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC380.10482180293501049No Hit
TTGTAAGGGCAGTCTGATAGATGAAAAATGTGTCCTCTAAGTATAATGTACT380.10482180293501049No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGA380.10482180293501049No Hit
AAGACTGGGATAAAATTTTCATTTGAGAATTTCTCAATTGTATCTAGTTAAA380.10482180293501049No Hit
AAATATGGGATGTATTAGCTGTGTAACAGATAAATCACTTAATCTTTTTGGG380.10482180293501049No Hit
AGTCCAGGGGCCCCTTACACCACCATGACAAACTACAAGTAAGTTACCATTA380.10482180293501049No Hit
ACTGCGGGGGATGACAATAGCTCTCAGCTGTCCTTTTTACAGGAGGTTGCAT380.10482180293501049No Hit
GGCAAGGTCCTAGGCATGGAGAAACTCCAAGCTCCTCTGTGAAGCAGGTTTT380.10482180293501049No Hit
GATAATGGGTCTGGTAGCTCAGTTAATAAAACAATTTCAGAATTAAAGAAAT380.10482180293501049No Hit
GTAACTGGGGTCCAAGGCTGAGTTGCAGTGAGTGACAATAATCTGTGCTAAT380.10482180293501049No Hit
CTTAGCGGGATCAGTTAAGTAGTTTTCGCAATGAAAATTTTAGACCATAGAT370.10206333443672072No Hit
TTTGGTGGGGTAAATATGGCTTAGCCTAATAAGAAGCTATTTTGAGAACTTT370.10206333443672072No Hit
CTAATTGGGTAGAGATGGGGTTTCACCATATTGGTCAGGCTGGTCTCGAACT370.10206333443672072No Hit
TTTGCTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC370.10206333443672072No Hit
AACATCGGGAATATCAAATTCACTTATGAATAAACTTGATAATGGAAACGGA370.10206333443672072No Hit
TGAACAGGGGGAAAGTAAGGAGATTGTTATCTATATCTAGTCTCCTTTCCAT370.10206333443672072No Hit
TGACATGGGGGAACCGCAGATTATGGTTAATCCAATTCTGTGCACCTGAGGT370.10206333443672072No Hit
GCTTTGGGGAGAAGAAGGAGAGCGAGCGAGCGAGCAAGGGAGCGAGCAGGAC370.10206333443672072No Hit
TGGTCAGGGAAGATTTCTCCATCTTTTAATTGTTGCAAGCTGTTTTTTTTGA370.10206333443672072No Hit
CACACTGGGGGTTTCTTTGAGGGTCACTCATTGAGACACGCAGGCCTCTGAG370.10206333443672072No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)