FastQCFastQC Report
Sat 31 Dec 2022
SRR5845274.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845274.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35370
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT2820.7972858354537744Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC2230.6304778060503251No Hit
ACGCCCGGGTTAATTTTTGTATTTTCTTAGAGATGGAGTTTCACTATGTTGG590.16680802940344924No Hit
GCTTTTGGGCAGTTTTCCCAACACCATTTATTGAAGACCCTGTCCTTTTATA530.14984450098953916No Hit
ACATGGGAGCGTTTTGCCAATTAAGCTCTTTGAGACTCAGCTCTTAAATTCA520.14701724625388746No Hit
CGTCTAGGGCTTAGATTCAGTGAGTGAACAATACATTTTTATTTTTTATTAT490.13853548204693242No Hit
AGGGAAGGGGACCGATAAGGATTCTTTTAGCTATTTTCATGAAAAAGTGGAA490.13853548204693242No Hit
CACCTTGGGACATATTTATTTTTCCTTTTTTCTTTCTATTCTATCATTATAC480.13570822731128074No Hit
CCATTGTGAAGTAAGAGATTAAGGCAAGTCAATATTCCTGACCACCTTCAAG480.13570822731128074No Hit
TCATAGGGGCGACTGTGTCATGACAAGGAAACTTACAAACTGCAACGCAGAG480.13570822731128074No Hit
GAGTTGGGGGAATTAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGT470.13288097257562906No Hit
TAAAGCGGGATCCTCTCTCACCATTTCTATTCAACATTTTACTGGAAGGCAG470.13288097257562906No Hit
GACCTAGGGGAGGAGGCAAAGGCAGAGGCAGAAGAGGGTGAGCAAACAAGGG470.13288097257562906No Hit
TGAACAGGGGGAAAGTAAGGAGATTGTTATCTATATCTAGTCTCCTTTCCAT470.13288097257562906No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTG470.13288097257562906No Hit
CACACTGGGGGTTTCTTTGAGGGTCACTCATTGAGACACGCAGGCCTCTGAG460.13005371783997738No Hit
TTTCACGGGTGCCCCTTCAAATGTTTCATCTAAAGGTGACAGCTTGTGGGCA450.1272264631043257No Hit
ATAACCGGGATAGAAAGATCTGTAACTAAATCTTTATTTTTAAGTGTTTATT450.1272264631043257No Hit
TCACAGGGGGATCTTGAAACGGGAAGAATAAAATAAAACTTAAAAAAAGAAT450.1272264631043257No Hit
ATTGCCGGGAGTGAGCAGTGTTGCGTTTTTCCTTGTAGCATTTGGAAATGAT450.1272264631043257No Hit
AATTATGGGAAATCAGTAAGACTAAAACAAAGGGCCATTTCCCATAAGCATA450.1272264631043257No Hit
GATAGGGGCACACCACGTCTTTTCAAAAACTAAAGAGAATTCAAAAAGGGCT440.12439920836867402No Hit
TCGTCTGGGGTATAAAAAAAGGGGTTTTAAAATGATGCCTACTTTTAATTTT440.12439920836867402No Hit
GATAACGGGATACCTCCCAGTCTGGAGATGAGATGACATCTCTGTCAGAGTA440.12439920836867402No Hit
TCAATCGGGGTAGCTTTTTTGACCATTATTTTTCCTTCATATCACTATATTA440.12439920836867402No Hit
CGTTATGGGAATGAAGATCGGGGGGATGACCAGCTGGAGTTGATCAAGGATG440.12439920836867402No Hit
GTTAATGGGCCGATCTGAACCAGGACACAAATTACTTATTAACATATGAAAA430.12157195363302234No Hit
TACACAGGGGTATGGAGGGTAATCTCTCTTTCTGTCCCCAATGTGCTTTTCT430.12157195363302234No Hit
GATAATGGGTCTGGTAGCTCAGTTAATAAAACAATTTCAGAATTAAAGAAAT430.12157195363302234No Hit
TCGCCTGGGAGTTCCACCTAGTGGCACACTCTTTCCTTTCCTCCTATCTCAT430.12157195363302234No Hit
GTAACTGGGGTCCAAGGCTGAGTTGCAGTGAGTGACAATAATCTGTGCTAAT430.12157195363302234No Hit
TCCGATGGGTAAGCAAAAGTCATTATTTTTCCTATTCATTTGAAATAAGTTA420.11874469889737065No Hit
AAGCAGGGGGCCCCGCCCGCTCCGGAGCAACCCGCGAGCTTACACCGGCTTC420.11874469889737065No Hit
AGCACTGGGAACTCAAAGACACAGAAAACTAGAGAAGAGAAACATCAGAAGG420.11874469889737065No Hit
CATACTGGGATAAATTAACATAAGTGTACTGTAACTTTACAAACGTTTTAAT420.11874469889737065No Hit
CCTTGGGGAGAACATTCTCAGATCTTTTCAGTCAAAAATCTAAGATGATTTA420.11874469889737065No Hit
TTACAAGGGGTGCACAAACTAGAAAACCTAGAAGACATGGATAAATTCCTGG410.11591744416171897No Hit
GTAACCCAAACCTCAGCATCGCACAATATACCCATGCAGCAAACCTGCACAT410.11591744416171897No Hit
AACTGTGGGGAGGGGTCTAAATCATCAAAGACCTAAATGATCGAGACTAGCT410.11591744416171897No Hit
CCTGAGGGGAATCATGTCTAACCAAGTAATAATGTTTTAGCAATAAAATTAC410.11591744416171897No Hit
GTTGCCGGGATAGCTATAAATTGAGTTTAACAATTTATAAACTCAAGAGAAT410.11591744416171897No Hit
GCTTTGGGGAAGTGGGTGGAGAATAACACAAACAATCTGGTGAAAGATTTGG410.11591744416171897No Hit
AAGCATGGGACACACTGTGCTGCAATCTACTGTTTGTGAATGTCAATGTATT410.11591744416171897No Hit
ACGAACGGGAAATGATGTCAAGGTAGCAACAAAACCCAAAAAATCCATCCTT410.11591744416171897No Hit
TTATATGGGGGGGTTGCAATCCTAGTTTCTGACAAAACAGACCTCAAACCAA410.11591744416171897No Hit
TCTATTGGGGGTGTGCGCAACCGCACCCAGCCTCAAATGACTTTAAAGTTTG400.11309018942606729No Hit
TTTGGTGGGGTAAATATGGCTTAGCCTAATAAGAAGCTATTTTGAGAACTTT400.11309018942606729No Hit
ATACTGGGGGATTTCTCTATCAGCACTTCCTTCTGCCTCCCAACTACTTGCC400.11309018942606729No Hit
TGACTGGGGGCTATAGCCTATGGGCAAAGAGGCAGTTCTTCTGGGTCTTGAC400.11309018942606729No Hit
GGTAATGGGCAGCGGAAGCTAATTTATTTATCAGTGATATTTAATGTTATGC400.11309018942606729No Hit
CGATGCGGGGATTAAAAACAGTAAGTACAGGTTAGTAATTACCTGGGTAATT400.11309018942606729No Hit
ATCGATGGAAAAGGAATGTTCTGTGGAAAAGGGAACTGGTGATTCACTAAGA400.11309018942606729No Hit
TATGCCGGGGGTGTTGCAGGAGGCTCCTCGACTTGGTTTCACAACCATAGTA390.11026293469041562No Hit
CGGAATGGGCAGCAATCTTGCATGAACTCTGCAGTAACTTCTGAAGACTCTT390.11026293469041562No Hit
AAATATGGGATGTATTAGCTGTGTAACAGATAAATCACTTAATCTTTTTGGG390.11026293469041562No Hit
CGTTATGGGTTATCTGATTATTATAATAAGGCAAAAACTAAAAATGAGTAAA390.11026293469041562No Hit
TAGCAACGGGGCCAAGAACTGATTTATTTATTTTTTTTTCTGAGATGACATT390.11026293469041562No Hit
TGCAAAGGGCTAGCAACATACCAGGTAACAAAATAATGTTTGTTGCAATGAA390.11026293469041562No Hit
GCACTTGGGGACAAAGAGGCATCTGGTCTTAACTGCTTGCTTCTAGTGGCCA390.11026293469041562No Hit
TCATCTGGGCCTGGGGACAATGTGGGCTTCAATGTCAAGAATGTGTCTGTCA380.10743567995476393No Hit
GGGCTAGGGAGTTTTTAATAGAAAGATCTATGTTTATAAACCAGCACTTGGC380.10743567995476393No Hit
AAGCATGGGAGTCAAGACAGAGATTAAGTAAAACAGGTTTTACTGTTTAGCT380.10743567995476393No Hit
TGCTTAGGGATTGGAACAAGTGTATTTTGTCTTAGACTAATGGTGATAACAG380.10743567995476393No Hit
AACTAAGGGTATAACAAACCCTGAGAACCAAAATGAACGAAAATCTGTTCGC380.10743567995476393No Hit
TGGGGCGGGAGTATTGCAGTTATACATGGGGGTTTTGGTACTTTATATTGTG380.10743567995476393No Hit
TACCTCGGGAGTTTTGAAATGCAACTTAAGCCATTAATTGTTGGTGTGAATT380.10743567995476393No Hit
CATATAGGGGGCCACCTGGCAGTGGCTGCCTTTAGGGCGGAGCCGGCTCCCG380.10743567995476393No Hit
CACAATGGGTGGGTCCTCTTCACACCTGATAGAAAAAATAAAATTCCTCACC380.10743567995476393No Hit
TTCACTGGGACTGACCAACCAACCAAACTCCACACTTCATCTGATCCCCCAT380.10743567995476393No Hit
GCTGTGGGTGCCCACCTCAAGTTTAATAAATAAGGTTGTACTTTTCTTACTA370.10460842521911225No Hit
CACTTCACAGAGGCTGAAACTGGGGCTAGTTATGGATGCTGGGCCCTGAACA370.10460842521911225No Hit
CGATACGGGGGGCGGCGAAGAGGACCCGCAGGCTGCAAGGAGCAACAGCGAT370.10460842521911225No Hit
TACATTGGGGCCGTTGGGGCCAAATAATGTTAACTGCATTGTTCTAGTTGCT370.10460842521911225No Hit
GTGCGCGGGACAGGAAGTACTGGAGAAAATGGACGTGTTATGACTCCAGAAT370.10460842521911225No Hit
GCAGCTGGGTCTGATTGATAGGCGTGAGCTGGCACCTCTTCAAGAATTAATA370.10460842521911225No Hit
ATAGTAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC370.10460842521911225No Hit
ATGAATGGGTACAATAAGCATATGCTTTGACTTTCCTTTGCTGTGGAGGTTT370.10460842521911225No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGA370.10460842521911225No Hit
CAATACGGGAACCTGAGAATGGCTGGATCATATGGGTAGATATATGTGTAAC370.10460842521911225No Hit
AAGACTGGGATAAAATTTTCATTTGAGAATTTCTCAATTGTATCTAGTTAAA370.10460842521911225No Hit
TGTTTATGGGGAAAGCTTGAAAGTAAACAGATTAGCAAATATGTATGTGTTA370.10460842521911225No Hit
TTTTTGGGGGGTGATATCAAATGAAATCCAGGCACAAAGCGGGAGCTTTGCA370.10460842521911225No Hit
ATATCCCTGGTGAATATAGATGCAAAAAACATCAACAAAATACTAGCTAACC370.10460842521911225No Hit
TTAGAGGGATGAAATCTCCAGCCATCACAGCCACCCTAGAGGAAAAAAATAG360.10178117048346055No Hit
ATGCAGGGGCAAAGTTACCAGAAGAAGAAGGACTGGGAGAGAAAACCAAGCT360.10178117048346055No Hit
GGTGTAGGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCT360.10178117048346055No Hit
TTAAAGGGGGTTGCTCTAAATTGACATTGTCTGTTTGTCTTTGAATCTATCT360.10178117048346055No Hit
GTCTAGGGGTCAGTCGGGTGTTGAAGTCTCCCACTATTATTGTGTGTAAATA360.10178117048346055No Hit
TTGTAAGGGCAGTCTGATAGATGAAAAATGTGTCCTCTAAGTATAATGTACT360.10178117048346055No Hit
ATTTAGGGGACTGCCAAGTCATCCGTGTCATTGCCCACACCCAGATGCGCCT360.10178117048346055No Hit
CTTGATGGGATTCCTGCACTGGTTGCCACCAACATTCTCTTGGTTGATGAGA360.10178117048346055No Hit
TAGCAGGGGATGGTGGGAGGAGCCGATACTGAGCTTCTTCCTATTTGCCATG360.10178117048346055No Hit
GTTCCTGGGGGAGCACTACCGGAAAGCTGGCTCTGTAGAGCTCCCAGCGCCT360.10178117048346055No Hit
GCTTTGGGGAGAAGAAGGAGAGCGAGCGAGCGAGCAAGGGAGCGAGCAGGAC360.10178117048346055No Hit
TTCTACGGGGGCCTCAGTTCATGCATGGCCATCCTGAGATTTGAGAGCCCAG360.10178117048346055No Hit
GCATACGGGAGTGTGATTTGATTCAATTCCATATAATGACAATTTTTGAAGA360.10178117048346055No Hit
CTTGTCACGGGGATGCTCTTGCACTAGTGAAATTGTCTAAAGAAACTAAAGG360.10178117048346055No Hit
TGCAATGGGTATTTCCTCGGATGCAGTGTAGCAAGTAGAAAGTGAAATACAG360.10178117048346055No Hit
GTATGAGGGACTTCAAAAAATAAAAACTGAAAACTTGTATTTCTTTGTTTTT360.10178117048346055No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)