FastQCFastQC Report
Sat 31 Dec 2022
SRR5845270.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845270.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64962
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT4470.6880945783688925Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC3090.4756626951140667No Hit
GGTTCTGGGGGTTGATAGGTGGGGGCGACTGGGCCAGGCGCCGGGGCAGGAA880.13546380961177304No Hit
TTATACGGGAGTTGGAAAATCCAACCCATCAGTTCTCTGGATATTTAGTTTT860.13238508666605092No Hit
ATGTATGGGATCAAAATGTATGCCTGGGTCAAAGCATTTTCTCAGAGTGTTC850.13084572519318985No Hit
TGCAATGGGCTTCTTCCTGAGAGGTAGTAAATACCAGGCTCTCCATCTGGAG820.1262276407746067No Hit
GCTAGTGGGGTGAGTCAAAAGAACAGGAATTCGGAAAACTCTTCAGTAAATA820.1262276407746067No Hit
CAAAAAGGGGTTGAAATGCTGTTTTCATTCTGCATTTGTGTAGTTTGGTGCT810.12468827930174563No Hit
AATCAAGGGCAGAGAAACTTACCCAAGCAACGTAATTCCTGTTTTCATGGGT800.12314891782888458No Hit
CATACAGGGGATTCAGATCAGAGGTTCGACTGTGGCTGGGACAGGAGTTGTG790.12160955635602352No Hit
AGTACCCGGGGAAATGAAATGAGATGAAAAATGATGAGATGAAAAATGAGAT780.12007019488316245No Hit
TCTGTGAAGAAATTAGTTTAATTATCCAGCTAATTCTGACTTTTTAAAACCT770.1185308334103014No Hit
AACATGCGGGGCCGGTGAGTTGGGTGCCGGTGGAGTCGTGTTGGTCCTCAGA770.1185308334103014No Hit
TGAGACGGGACCATTGGCTGGGGGCCAGCTTTCTTTTCCTACCAGGACTGCA770.1185308334103014No Hit
TGCCTTGGGATGTGAATTTTAACTGAACTCTGAGATCCAGAACACAAGCGTT770.1185308334103014No Hit
GATTGTTTAAAAGTTTGTGGCACTCCCCTCTCTCTTCCTCCTGCTTCAGTCT760.11699147193744036No Hit
TGGCTCGGGGGGCAAAGGATATAAACAGACACTTCTCAATAGTCTACTAATT760.11699147193744036No Hit
GAACCTGGGATTTCAATTTTAATGTGTCCTCTTTAGTTTCGTTCATCAGTGT760.11699147193744036No Hit
TCTCGAGGGAACAGAGCATGGCATAAAGTGGCTACTAAGTAAATGAATAATG750.11545211046457929No Hit
TTTCCTGGGGTTTAAAGTTTTTTAAAAAAATTTTATTTAAATTGGTTTTGAG750.11545211046457929No Hit
GAGTCCGGGTTATTTTAGAATGATAGAGGGTTCACAACTACTAGTCTTGTAG740.11391274899171823No Hit
GTGACCGGGGCAGTGAACAACCTCTGGTTTTGTCGGACACCATTTTGGAATA720.11083402604599613No Hit
TAATAAGGGGTTCATGTTCAAATGTTCACAACCACAATGCATCTGACTGCAA720.11083402604599613No Hit
CCAAACGGGATCTTGTTATGTCTTTGTATCTCAGAGAAAATAAGTTAGAGAG710.10929466457313505No Hit
CTCTCTCTGGGGTTAGCAACATTGCAGATGATAGACAGGTGAAAGATAGATG710.10929466457313505No Hit
ACGCTTGGGGGTTTTCTACTTTGTGTTGGGAATGCTTCTGGTTAGAAGCTGG700.10775530310027401No Hit
AGCTGAGGGTCTCCCAACTCTGCTAATTTCAAGGTTAATGAGGTTGAAGATT700.10775530310027401No Hit
GTACATGGGAGGCAGTTTCTGGAAAACCCATTATTGAAGCCATAGGCTGTGG700.10775530310027401No Hit
TTCTGGGGGATATGTATGTGTAAATAAAATTAAATTAATAAAATTAGGGAAA690.10621594162741295No Hit
AATAGTGGGATCTTTGATGTTACTACTGTTATTACTCTGGGGCATGATGAGC690.10621594162741295No Hit
AGATAAGGGGGGATTGCTGGGTTGAATGGTATTTCTGTCTTTAAGTCTTTGA690.10621594162741295No Hit
TCAATAGGGATGGAACTGGTCCAAGTGTTTATAAAGGTTTAGTCCCTAAACC690.10621594162741295No Hit
TACCAAGGGATAAAGAGTGCTACATGGTTTAGATAAAGGAAACATATAACTA690.10621594162741295No Hit
ATTTCAGGGGTATAAAAAATTATCTCACATCTGATAAAATTTTGATTTTTCC680.10467658015455188No Hit
AAGTTTGGGGGAAGTTTAAAAAAATAACATAGTTATCTTTTACTTTTATATG670.10313721868169083No Hit
ATAGCAGGGCCATGCTTTCAGGGATCCAGAAAATAGTGTGTTACAAAATTTG670.10313721868169083No Hit
AAAAACGGGGAAGTTCTAGTTATTTCTGGAGTTGCTGTGGATCTACAGATAC670.10313721868169083No Hit
GCATTTGGGGTGTACATCCAGCAGTTACAATTTTTAAAAATTATACTTTTAC660.10159785720882979No Hit
GGACTAGATCATTCCCGTAGACAGTTCCATTGTGCACACATAGTATTTCGTT660.10159785720882979No Hit
AAAATAGGGATTAATGAAGTTCCTTAAGAGACCACTCTTAATGAAGTTCTCT660.10159785720882979No Hit
GAATGTTGGGGAAAAGAAAGAGCTAAATTGCCTTCCAGTTCTTTGAAGTAAA660.10159785720882979No Hit
GTTCAAGTCCACATGAGATATTTACCAAGATAAACCATATTCTGTATTATAA660.10159785720882979No Hit
ACTGCAGGGATTTATAATAAGTTGTGTAGTTTGTCCTGCATCCATACTACAC660.10159785720882979No Hit
TACCTGGGGAACAAGAGGAACTTTGGAAACTGTACAAATGCATGGAAATTAA660.10159785720882979No Hit
GTGACAGGGAGCTGACTCAGAAAAAGCAGGAAGTTCAGAGACGAGAGCGGGC660.10159785720882979No Hit
CGTAATGGGGTCCCAGATGTCCTTGACATGAAAAACAGCACAAAAATTCTCC660.10159785720882979No Hit
TGCTGCCGGGGTATCAGTCTTTTTGGCTTTTCTGTTACCACCACTGCCTCTA650.10005849573596873No Hit
TGACACGGGGGTTTGGCCACTGAATTAATACTGTATAGCAAGAGAAACAATC650.10005849573596873No Hit
CAATGCGGGTATGCTTGTCTAATTTTTTGCAGAGTATGATGAAGTTTTCTTG650.10005849573596873No Hit
TATCGTGGGCGGCTCGGGGGTTGGAGAGCTGAGCATGTGTGTGGCTTTAGTG650.10005849573596873No Hit
TGGTTTGGGTTACTTTGAAAGTGCCAGAGTAGAGTGTGCAATACAAACATGT650.10005849573596873No Hit
CGAACAGGGGCATGTAAGTGCTTAGCACCATTTTGCATTTCTTTAGCTTTTA650.10005849573596873No Hit
GCATCGGGGATGCCATTAAAAGTCTAATAATAAATAGCCAAAGCTAACTTAA650.10005849573596873No Hit
GGTTCAGGGTCTTGGTGAGGGGTTTTTCATTAGTGAATTTAGACCTCTTTGA650.10005849573596873No Hit
AACATCGGGTATTGCTGCTGCCTGAAAGGTACAAATTGCTTGGGTGTAGGCT650.10005849573596873No Hit
GAATCTGGGCGATCTTGCGGCGGGAACCCCAAAGGCAGGTGCCATCACATAC650.10005849573596873No Hit
AAGTTCGGGAATTTTAATTTACATTAACTTTAAAATATTTGTATGCCAAACA650.10005849573596873No Hit
ATACCCGGGATCGAAACCGCAAACATATCATACACAAACGCCTGAGCCCTAT650.10005849573596873No Hit
CTTACTGGGGTCCGTTTCTTAAAATTATGATTAGTGAGTGGTCTAACAGTTT650.10005849573596873No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)