FastQCFastQC Report
Sat 31 Dec 2022
SRR5845269.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845269.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65027
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT4310.6628016054869516Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC3160.48595198917372784No Hit
AATCAAGGGCAGAGAAACTTACCCAAGCAACGTAATTCCTGTTTTCATGGGT1020.1568579205560767No Hit
TGCAATGGGCTTCTTCCTGAGAGGTAGTAAATACCAGGCTCTCCATCTGGAG980.15070662955387762No Hit
ATGTATGGGATCAAAATGTATGCCTGGGTCAAAGCATTTTCTCAGAGTGTTC850.1307149337967306No Hit
CTAATGGGGGTTGCACTCTTTTTCAGTGACCTAAAAATGGCTTATTTCCGAG850.1307149337967306No Hit
GTGACCGGGGCAGTGAACAACCTCTGGTTTTGTCGGACACCATTTTGGAATA800.12302582004398172No Hit
ATCTATGGGGCCAAGCACCAGCTTGGTTGTGGGCTGTTTCCTTCAGTGACTG800.12302582004398172No Hit
CTACAAGTGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATT790.12148799729343196No Hit
ACACCGGGCATCATTGTATCTTCACCATCTAGCACGGTGCTAATCATATAGA780.11995017454288219No Hit
TAATTGGGGAACTTTTAAAAATCATTTATAGAAATTATTTTGGGGGAAAGAG770.11841235179233241No Hit
AATAGTGGGATCTTTGATGTTACTACTGTTATTACTCTGGGGCATGATGAGC770.11841235179233241No Hit
CAATGCGGGTATGCTTGTCTAATTTTTTGCAGAGTATGATGAAGTTTTCTTG770.11841235179233241No Hit
TGAGACGGGACCATTGGCTGGGGGCCAGCTTTCTTTTCCTACCAGGACTGCA770.11841235179233241No Hit
GTTATGGGGGAATAAGTTTGCAGAAAAAACTTCAGCATGTGCCAGGAACACA770.11841235179233241No Hit
TGGCTCGGGGGGCAAAGGATATAAACAGACACTTCTCAATAGTCTACTAATT750.11533670629123288No Hit
CAAAAAGGGGTTGAAATGCTGTTTTCATTCTGCATTTGTGTAGTTTGGTGCT750.11533670629123288No Hit
ATTCGCGGGCGATCTGATCACGGGAAGACGAACTGGAGAAAGCATTAAGAAA750.11533670629123288No Hit
TTATACGGGAGTTGGAAAATCCAACCCATCAGTTCTCTGGATATTTAGTTTT740.11379888354068311No Hit
AGATAAGGGGGGATTGCTGGGTTGAATGGTATTTCTGTCTTTAAGTCTTTGA740.11379888354068311No Hit
GAGTCCGGGTTATTTTAGAATGATAGAGGGTTCACAACTACTAGTCTTGTAG740.11379888354068311No Hit
CTTGTTGGGGAGAATGAGACTTTCGGGTCCTCAAGCTTTTGATAAAAATGAA730.11226106079013333No Hit
AAGGTTGGGCTGTTCTGAAATAAAACCATTTCCTTATGTTTAATAGGCAGAT730.11226106079013333No Hit
TCAAGAAGGGAAGAAACAAAACAACTACATGATATTTGTAGAGTATCAGAAA730.11226106079013333No Hit
GTTTCTGGGAATGTTCAAAGACAGCAAACTTCAACCTGTGGGTCAAATCTGG720.11072323803958356No Hit
AAGTCGGGGGATTGGTCCCATTTGCCCTCAGGTCAGTTGTTTAATCTCAAGA720.11072323803958356No Hit
TAATAAGGGGTTCATGTTCAAATGTTCACAACCACAATGCATCTGACTGCAA710.10918541528903379No Hit
CAATCTGGGTTATAGGAAGAGGAAGTAAAAGAATCCCTATAATTTAGCAAAT700.10764759253848401No Hit
TGCCGGGAGAACTTCTTCGTTATTAACCTTGTAGCTTCCATCTCGCCTAAGA700.10764759253848401No Hit
CATGCTGGGGGCCCTGTCTGGAACTTGGCATGATGGTTCTGTTGCGACCAGC700.10764759253848401No Hit
TCTCGAGGGAACAGAGCATGGCATAAAGTGGCTACTAAGTAAATGAATAATG700.10764759253848401No Hit
CAACATGGGAGTCCCCGACTTAACGACGGTTCAATTTGGCAATTTTTCTACA700.10764759253848401No Hit
CTAGTAGGGTGCATTTATTATTTTGTCTTTCTGTTTCATATACAAAATGAAC690.10610976978793424No Hit
CGAACAGGGGCATGTAAGTGCTTAGCACCATTTTGCATTTCTTTAGCTTTTA690.10610976978793424No Hit
CTGTAGGGGGGTAATAGTCACAATGAAAATTTTTATTCAGGTTTAATTATAA690.10610976978793424No Hit
TACATGGGGTAAGATATGTGATGCTAAATTGTTGCTTCATAATCTGAAGAAG690.10610976978793424No Hit
ATTCAAGGGTGGAGATTCATAGATATGTCTTTTAATGTAATATATGTCCTTT690.10610976978793424No Hit
AAAATAGGGATTAATGAAGTTCCTTAAGAGACCACTCTTAATGAAGTTCTCT680.10457194703738448No Hit
CGTATTGGGAATCAGACTAAACTATAGCGATGAATCACCTGAAGCTGGTAAA680.10457194703738448No Hit
TGTTGCGGTGAGCTGAGATCGTGCCATTGCACTCCAGCCTGGGTAACAAGAG670.10303412428683469No Hit
AGCTGAGGGTCTCCCAACTCTGCTAATTTCAAGGTTAATGAGGTTGAAGATT670.10303412428683469No Hit
TATGCAGGGATCTCACGTGCAGAGACACACATAGGCTCAAAATAAAGGGATG670.10303412428683469No Hit
GTACATGGGAGGCAGTTTCTGGAAAACCCATTATTGAAGCCATAGGCTGTGG670.10303412428683469No Hit
GTGGTGGGGATAGCAAAAATGATCTCGAAAAAGAATAAAATGTGAGTAATCA670.10303412428683469No Hit
CCACTAGGGGGGAAATTAAATGATATGGACCCTAATTTGTATCATTCTGAGT660.10149630153628493No Hit
GGTTCTGGGGGTTGATAGGTGGGGGCGACTGGGCCAGGCGCCGGGGCAGGAA660.10149630153628493No Hit
TTTATCGGGATTTTAAAGTTGGGCTAATAGGTTTTAAAAGTCTCTTAAGTAG660.10149630153628493No Hit
AAATGTGGGTGTCAGAAGAAGGATGGTGGTGAGTGGTCTTTGTCTGCCTAAA660.10149630153628493No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTG660.10149630153628493No Hit
GAACCTGGGATTTCAATTTTAATGTGTCCTCTTTAGTTTCGTTCATCAGTGT660.10149630153628493No Hit
TTGCGTTGGGAGGCATTTCTTATATCTGTGAACATGTGCACCCTTTGTATAT660.10149630153628493No Hit
CCATAAGGGTGGACTCATTCCATCGGTAATCCCTCTCTCACAAAAGAATAAA660.10149630153628493No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)