FastQCFastQC Report
Sat 31 Dec 2022
SRR5845268.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845268.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64280
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT4230.6580584940883635Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC3170.49315494710640945No Hit
GAACCTGGGATTTCAATTTTAATGTGTCCTCTTTAGTTTCGTTCATCAGTGT870.13534536403235845No Hit
AGCTGAGGGTCTCCCAACTCTGCTAATTTCAAGGTTAATGAGGTTGAAGATT840.13067828251400124No Hit
GGTTCTGGGGGTTGATAGGTGGGGGCGACTGGGCCAGGCGCCGGGGCAGGAA800.12445550715619166No Hit
CTTGTTGGGGAGAATGAGACTTTCGGGTCCTCAAGCTTTTGATAAAAATGAA780.12134411947728686No Hit
GCATTTGGGGTGTACATCCAGCAGTTACAATTTTTAAAAATTATACTTTTAC780.12134411947728686No Hit
GCTAGTGGGGTGAGTCAAAAGAACAGGAATTCGGAAAACTCTTCAGTAAATA770.11978842563783447No Hit
TGGAGCGGGGATGTAATAAAATATTTTTAAATTTTGACATAGTGATCATTTA760.11823273179838209No Hit
CTCATTTGGGATCATAAGTATACAGCTTGATGACTATCAGTATGTTTCTTAT750.11667703795892967No Hit
TGCAATGGGCTTCTTCCTGAGAGGTAGTAAATACCAGGCTCTCCATCTGGAG750.11667703795892967No Hit
ATGTATGGGATCAAAATGTATGCCTGGGTCAAAGCATTTTCTCAGAGTGTTC750.11667703795892967No Hit
GTTATGGGGGAATAAGTTTGCAGAAAAAACTTCAGCATGTGCCAGGAACACA750.11667703795892967No Hit
GCTTTGGGGGCAAATTGGGAGGCCTCCCAATTATTGCAAACATGATCAACAA720.11200995644057249No Hit
AAGTTTGGGGGAAGTTTAAAAAAATAACATAGTTATCTTTTACTTTTATATG710.1104542626011201No Hit
CAATGCGGGTATGCTTGTCTAATTTTTTGCAGAGTATGATGAAGTTTTCTTG710.1104542626011201No Hit
TATCGTGGGCGGCTCGGGGGTTGGAGAGCTGAGCATGTGTGTGGCTTTAGTG700.10889856876166772No Hit
TAGTTTGGGACGCTCTTGCGATCTTTTGCGGGGGTCCATTTTTTTATGTAAT700.10889856876166772No Hit
CCATCAGGGGGACACCAAGCACAAAAGAGGTCTAAAAATGGAGATTAAGTGA700.10889856876166772No Hit
TGCCTTGGGATGTGAATTTTAACTGAACTCTGAGATCCAGAACACAAGCGTT700.10889856876166772No Hit
CATACAGGGGATTCAGATCAGAGGTTCGACTGTGGCTGGGACAGGAGTTGTG700.10889856876166772No Hit
TGACACGGGGGTTTGGCCACTGAATTAATACTGTATAGCAAGAGAAACAATC690.10734287492221531No Hit
TTCTATGGGACCTTCATAAAAAGCTATTTTATATTCAGTTGTTTTGTTTGTT690.10734287492221531No Hit
TTCATGGGGGAATAGGGGAAACCACATACTGCCCATTCCTCCAGGATCATTT690.10734287492221531No Hit
ATCTATGGGGCCAAGCACCAGCTTGGTTGTGGGCTGTTTCCTTCAGTGACTG690.10734287492221531No Hit
GACTAGGGGGAACTGGAGATTCCTGACCAGAACATTGATGTAGAAGGTAACT690.10734287492221531No Hit
CTAGTAGGGTGCATTTATTATTTTGTCTTTCTGTTTCATATACAAAATGAAC680.10578718108276292No Hit
ATTCGAGGGAGGCCCCAGCACACTCAGAGACAGACCTCAGGATATCATTGTG680.10578718108276292No Hit
TGTCAGGGGGTCTAAACTTAACTTAAATGGTTTTTTACAGGAGTTAAAGTAC680.10578718108276292No Hit
CAATCTGGGTTATAGGAAGAGGAAGTAAAAGAATCCCTATAATTTAGCAAAT680.10578718108276292No Hit
GACAATGGGGAAATGAAGGAAAGAAAGAAGAGAAGAACACAAAACAACCCAA680.10578718108276292No Hit
CAACATGGGAGTCCCCGACTTAACGACGGTTCAATTTGGCAATTTTTCTACA680.10578718108276292No Hit
TACCTGGGGAACAAGAGGAACTTTGGAAACTGTACAAATGCATGGAAATTAA680.10578718108276292No Hit
AATGATGGGGAAATGTCTCCAGCCTTTGATCTAATGACTAATTTTAGGCATC670.10423148724331051No Hit
AATAGTGGGATCTTTGATGTTACTACTGTTATTACTCTGGGGCATGATGAGC670.10423148724331051No Hit
TGCCGGGAGAACTTCTTCGTTATTAACCTTGTAGCTTCCATCTCGCCTAAGA670.10423148724331051No Hit
TGCTCAGGGTAATTTTGGATGGTTTAACTGACTGTCCTGAACTGACTGATCC670.10423148724331051No Hit
AAAAACGGGGAAGTTCTAGTTATTTCTGGAGTTGCTGTGGATCTACAGATAC670.10423148724331051No Hit
GAGTCCGGGTTATTTTAGAATGATAGAGGGTTCACAACTACTAGTCTTGTAG670.10423148724331051No Hit
ATGTGTGGGGTGCCGTCTTTGGTTCCTCATGGCTGTTATCTGTCTTTATGAT660.10267579340385811No Hit
CAAAAAGGGGTTGAAATGCTGTTTTCATTCTGCATTTGTGTAGTTTGGTGCT660.10267579340385811No Hit
GTGTTCGGGACACCCACCGGGAGGTGGCCCTGAATGATGGTGCCACCTGTGC660.10267579340385811No Hit
AAAACTGGGGTTGAAGGCCAATTTGTGGACTCCTACGATCCAACCATAGAAA660.10267579340385811No Hit
CTACAAGTGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATT650.10112009956440574No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTG650.10112009956440574No Hit
TTGTGAGGGACCTAGCACACTTCCTTCTCCCACCCCTTTTTCCAGTTGGATT650.10112009956440574No Hit
TTTTTAGGGATAAAAGAGTTTATTATTTTTTATCATCTTGATCCTGACAATC650.10112009956440574No Hit
TCAAGAAGGGAAGAAACAAAACAACTACATGATATTTGTAGAGTATCAGAAA650.10112009956440574No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)