FastQCFastQC Report
Sat 31 Dec 2022
SRR5845261.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845261.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48370
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT4971.0274963820549927Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC3240.6698366756253876No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTG740.15298738887740335No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCTTATAC700.1447178002894356No Hit
CACACTGGGGGACCATTGAGGATGATAACATAGGGTGTCTTAGGTGGTTGTC680.14058300599545173No Hit
CTGCGTGGGTCACCATCACCTCAACACCAACACCATCATCACCACCATCACC630.13024602026049203No Hit
GTGCTTGGGCTAGCTTTGGGGTAGCACAGAACTGCACTAGGATTAATTTGTT580.11990903452553235No Hit
CATCAAGGGCACATTGTCAAACAGAATTTTTCTTTGAATCAGACAAGTTCAA580.11990903452553235No Hit
AGGGCTGGGATCTCAACTGCGGGGACTGGAAATGCTAACTAAAAACTGTCTT580.11990903452553235No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCT570.11784163737854042No Hit
ATACAAGGGTACAGGCATATGGCAGGAGAACTTTCAAGGACTTTAATTTTGT570.11784163737854042No Hit
GCTTGCGGGCAACTACTCAGCAGCCACATGTGGCTAGTGGCTGCTGCAATGA560.11577424023154848No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGA560.11577424023154848No Hit
GTAGGCGGGAAGGAAGAATCTGTGAAGCTATTCCGAGGGGTGAAGAAGATGG550.11370684308455653No Hit
TTTGCCGGGGCATCGAACGTCAATAAGCACATTCAAGAAACTGAATGGCAGA540.1116394459375646No Hit
TGGACTGGGATCTCTTTGGAAACTACATCGAGAACAGAATTCCCATGGAACG540.1116394459375646No Hit
ATGCTAGGGGTATAAGTGTTAAATTATGAAATTTAGTGGTCTTATAGAGAAT540.1116394459375646No Hit
TGCTTCGGGCAAGAAGAAAGGATCTGTGAAAATCACTTTGAATTCAGACTTC530.10957204879057268No Hit
GCTCTGGGATTATTCATGCAGTAGTTTTTAAACTCTATTTTATGAAACTCTA520.10750465164358074No Hit
TAGCATGGGAAATGGGAAGGAAGAGAGAGGTTATTAGCTTTTCATAGAAACT520.10750465164358074No Hit
GAGCCAGGGAAACCAGTTAGAGAGCAACAGATGACTTAAGCTTTGATAGTCA520.10750465164358074No Hit
AGTCTGGGGGTAGTCGCCGCCGCCACCGCCGCTGCAGCCACTGCAGGCACCG520.10750465164358074No Hit
CCATAGGGGGTAACCTCTGCTTTAGGGAATTCAAGTCTTCTCCAGATTGTTC520.10750465164358074No Hit
CTGATAGGGAATTTTTTAAAATTGAAGTAAAATTCATATATCATGAAAGTAC520.10750465164358074No Hit
CTAAGAGGGAAGTCACCACCAGTCCTTCTCTTTTTCAAAATATATCATCCTA510.1054372544965888No Hit
TCTGTCGGGGATGCCATGTACCAAATTCAGAAGCTATCGTTAATAATTTGTT510.1054372544965888No Hit
TCGCATGGGATTTCACTTGGTTTTATTAGTACAAAATTGTGCTTTATATAAT510.1054372544965888No Hit
TCAGTCGGGGAGACTGGGTGGATGAGGCTCACCCCGGCGGGGAGAAGGGACG510.1054372544965888No Hit
CGCCAAGGGACCAATAGGTTATCTGTCAAATTAATCACTGACAAAACTTGAT500.10336985734959686No Hit
CAAAACGGGAAGTACTCCAAATTTAAATATTTAAGTCTATGATATTGATATT490.10130246020260492No Hit
GTTTGTGGGGATGATGAAGCCTTCAACTTTGCATATTCATGTACTCACTCTC490.10130246020260492No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)