FastQCFastQC Report
Sat 31 Dec 2022
SRR5845260.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845260.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48638
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT4570.9395945556971916Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC3190.6558657839549323No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTG870.17887248653316337No Hit
CACACTGGGGGACCATTGAGGATGATAACATAGGGTGTCTTAGGTGGTTGTC840.17270446975615775No Hit
GCTTGCGGGCAACTACTCAGCAGCCACATGTGGCTAGTGGCTGCTGCAATGA700.14392039146346478No Hit
ATACAAGGGTACAGGCATATGGCAGGAGAACTTTCAAGGACTTTAATTTTGT700.14392039146346478No Hit
ATTTCAGGGATGGGAGGTGCTATGGGCATAAACAACAGAGGTGCCATGCCCC650.13364036350178873No Hit
CTGCGTGGGTCACCATCACCTCAACACCAACACCATCATCACCACCATCACC650.13364036350178873No Hit
TTACGTGGGACAGTGAGAATGTTGTTTTTCCCCTAAACATGCCCCAAATTTA630.1295283523171183No Hit
GCGCAAGGGAGACATGTTTTGGATAAATCATGGCTGCTTGAAAAAGAAATCC620.12747234672478308No Hit
AGGCCGGGATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCT610.12541634113244787No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCTTATAC610.12541634113244787No Hit
AGTGCCGGGGAGCAGCATTATCCCCAAAGATATTACAGGGTAGACACGTTTT600.12336033554011266No Hit
CTTAATGGGAGATACAGCCACAGCACACTCCTGGTTCGTAGCTATCCACACC600.12336033554011266No Hit
TTTTGAGGGCTCCCAAAAAGGTTTTTGTGGGTGGATTGAGCCCGGATACTTC600.12336033554011266No Hit
AGTATAGGGTATTTTGGATGTGTCAAACGTTAAAAATTAATTTCACCCATTT590.12130432994777746No Hit
GCAGTGGGAATTATACACTGATAAATTGGAATAACCTAGAAAAAATGGATAT580.11924832435544225No Hit
TGGACTGGGATCTCTTTGGAAACTACATCGAGAACAGAATTCCCATGGAACG580.11924832435544225No Hit
ATTTATGGGAGGATGCAAGAAAAGGAGTGAGGAATGGGAAAATTGGAAGAAC570.11719231876310704No Hit
TGCTTCGGGCAAGAAGAAAGGATCTGTGAAAATCACTTTGAATTCAGACTTC560.11513631317077182No Hit
TTCGAGGGGAATCAAAACTTTCTTTCATTAGCTGTGTCTCTGCATCTAAATT560.11513631317077182No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCT560.11513631317077182No Hit
GAAAGTGGGAAGGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACC550.11308030757843661No Hit
ACTTGGGGCCAGCTCCAGACTGTTATTCACAAATGGTGTGAATGTTGTGACA540.1110243019861014No Hit
AACCATGGGCGATCTGTCGTGGGGGAACTGGTATGGTTTGGCAGAATAGTTT540.1110243019861014No Hit
AAGTCCGGGAAAACCAACTGATGATAAGAATGTAGAGCAACAAGAACTCTCA530.10896829639376619No Hit
AACGCTGGGAGAGGACAGAATAACTCTCTGGTTACCAGATGTGAGGCAATTA530.10896829639376619No Hit
ATCAACGGGTCGGTGGGGGATATCCAAAATTGCATCCTAATTATAAAACAAG520.10691229080143098No Hit
GCAACGGGAGGAGATAAAACCCCCACTGAGATGCCTCTCATGCCTCAGCTGG510.10485628520909578No Hit
GGCATTGGGCACCCAGGTGTTCTTTGTGCATACAAAAAATTTGTTTGGGTCT510.10485628520909578No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGA510.10485628520909578No Hit
AGTCTGGGGGTAGTCGCCGCCGCCACCGCCGCTGCAGCCACTGCAGGCACCG510.10485628520909578No Hit
TTACATGGGTTGCTACTCCAGGCCGGCTGTGGGAATTAATTAAAGAAAAGCA500.10280027961676057No Hit
TGCTAAGGGCGGTGCCAGCGCCTGCCTGCGCGCTGCTGCTGCTGTTTCCCCT500.10280027961676057No Hit
AAAGGAAGGGCCGTGGAGGAAGTAACTGCAGATGTGGTAGAAATAGCTAGCA500.10280027961676057No Hit
AAAATAGGGATTATCCCATTTCTTCTCCTCATGGTGTGCGTTCTGTTTTGTA500.10280027961676057No Hit
TTGCAGGGTAGCCAGCCTCTTTTCTAGCTTTTTCTTAGTAACAGGTGGGATC500.10280027961676057No Hit
GTTTGCGGGGAAATCATCTAATGATGCATTTTTCAGAAAGTATCACCATCAT490.10074427402442533No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)