FastQCFastQC Report
Sat 31 Dec 2022
SRR5845259.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845259.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47314
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT4730.9997041044933846Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC3230.6826732045483367No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTG880.1859914613010948No Hit
AGGCCGGGATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCT680.1437206746417551No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCTTATAC650.13738005664285413No Hit
ATACAAGGGTACAGGCATATGGCAGGAGAACTTTCAAGGACTTTAATTTTGT650.13738005664285413No Hit
TGCTTCGGGCAAGAAGAAAGGATCTGTGAAAATCACTTTGAATTCAGACTTC630.13315297797692016No Hit
GCTTGCGGGCAACTACTCAGCAGCCACATGTGGCTAGTGGCTGCTGCAATGA590.12469882064505221No Hit
TTGCAGGGTAGCCAGCCTCTTTTCTAGCTTTTTCTTAGTAACAGGTGGGATC590.12469882064505221No Hit
ATTTATGGGAGGATGCAAGAAAAGGAGTGAGGAATGGGAAAATTGGAAGAAC560.11835820264615125No Hit
TGGGCGGGAAGTGAAGTTATATCAGTCTGATCATGAAACCTCAACTGGTTAC550.11624466331318427No Hit
CTATTAGGGGTAGAATAACCCAAAGACAAGAACACTGAATAGACTAGGAAGT550.11624466331318427No Hit
TTACGTGGGACAGTGAGAATGTTGTTTTTCCCCTAAACATGCCCCAAATTTA550.11624466331318427No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCT550.11624466331318427No Hit
TGCTAAGGGCGGTGCCAGCGCCTGCCTGCGCGCTGCTGCTGCTGTTTCCCCT540.11413112398021728No Hit
CACACTGGGGGACCATTGAGGATGATAACATAGGGTGTCTTAGGTGGTTGTC540.11413112398021728No Hit
CTGCGTGGGTCACCATCACCTCAACACCAACACCATCATCACCACCATCACC540.11413112398021728No Hit
ATTTCTGGGAGGCACATAGATCAGTAGAACAGAATAGAAAGCTCAGAAATAG530.1120175846472503No Hit
TGCCAGGGGATGTTTCCTGCCCAAAGGACACATTTGGGCAGTGCCACCCACT530.1120175846472503No Hit
AGGGCTGGGATCTCAACTGCGGGGACTGGAAATGCTAACTAAAAACTGTCTT530.1120175846472503No Hit
CGCCAAGGGACCAATAGGTTATCTGTCAAATTAATCACTGACAAAACTTGAT520.1099040453142833No Hit
GCGCAAGGGAGACATGTTTTGGATAAATCATGGCTGCTTGAAAAAGAAATCC520.1099040453142833No Hit
TGGACTGGGATCTCTTTGGAAACTACATCGAGAACAGAATTCCCATGGAACG520.1099040453142833No Hit
CATCAAGGGCACATTGTCAAACAGAATTTTTCTTTGAATCAGACAAGTTCAA510.10779050598131631No Hit
AGTCTGGGGGTAGTCGCCGCCGCCACCGCCGCTGCAGCCACTGCAGGCACCG510.10779050598131631No Hit
AAACACGGGAGATATTCCCAGTGTCTGTCTGATAATATTTTGCATCTAAGAA500.10567696664834933No Hit
AAGTCCGGGAAAACCAACTGATGATAAGAATGTAGAGCAACAAGAACTCTCA500.10567696664834933No Hit
ATAAAGGGATTAAAAGCAGGAGGTAAGAATTCATCATTCCATCAAGTAGAGA500.10567696664834933No Hit
GCAATGGGAGCATATGACTTTTAAAAATATTGCTGTAGAGTAGGCAAAGCCC500.10567696664834933No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGA500.10567696664834933No Hit
GCTGTAGGGAACTACAAATATGGCAAGATCCAGGGGTGGTATAAAGACAAAA490.10356342731538234No Hit
CAGCAGGGGGTATCTTCCAGAGTTACTGTTTAGAGATGTGAAAATGTTGAAA490.10356342731538234No Hit
TCAGGCGGGAGGTCTCACCTGCTAAAGATTCTGGTTCATTTTAGCTGGATAG490.10356342731538234No Hit
AAAGGAAGGGCCGTGGAGGAAGTAACTGCAGATGTGGTAGAAATAGCTAGCA490.10356342731538234No Hit
TCAGTCGGGGAGACTGGGTGGATGAGGCTCACCCCGGCGGGGAGAAGGGACG490.10356342731538234No Hit
CAATGAGGGATGTGCTGAAAATCCGAAGTGCCGCGGAAAGTGGAGGTGAGGG490.10356342731538234No Hit
AGTGCCGGGGAGCAGCATTATCCCCAAAGATATTACAGGGTAGACACGTTTT480.10144988798241536No Hit
GATGCTGGGATAGGAATGGTGTGTGTCGGGCAGATGATCCCTTTGACTGCCT480.10144988798241536No Hit
TGTGAAGGGATTTCAAGGTCAAGATGAACTGAAGGGGAAGAGAAGTAAAACT480.10144988798241536No Hit
AGGTTCGGGATGGAAATATATTTTTGAAGTGTATTAACAATATAAAAATTAA480.10144988798241536No Hit
GTCAAGGGAATTGGAAATATTTAGGGTTTAAAGGTAGAAGGAATTCAGTTAT480.10144988798241536No Hit
AGTACTGGGTATCGAGGGAGTGGGAAAAAGTGAAGTCCCTATTTGAAGAACT480.10144988798241536No Hit
TTTTGAGGGCTCCCAAAAAGGTTTTTGTGGGTGGATTGAGCCCGGATACTTC480.10144988798241536No Hit
CTGATAGGGAATTTTTTAAAATTGAAGTAAAATTCATATATCATGAAAGTAC480.10144988798241536No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)