FastQCFastQC Report
Sat 31 Dec 2022
SRR5845258.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845258.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52283
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT3160.6044029608094409Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC2740.5240709217145152No Hit
TACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGG970.1855287569573284No Hit
CTTAGGGGGGGAAATTCTCATTCCTTGCTGCTGCAAGTGTGGAACAGTACAA830.15875141059235315No Hit
CTGAACGGGAAATGAGAATGGCTGACCTCTCGGAGCTCCTGAAGGAAGGGAC800.15301340779985848No Hit
GACTGCCGGGGGATGAAGCCCGGGGCAAGAACTGTGGTGTCTTGGTACATTG790.15110074020236022No Hit
CAAAATGGGGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCT780.149188072604862No Hit
TATTAGGGAGCCAAACTAAGCTTCATAAGTGAAGGAGAAATAAAATCCTTTA780.149188072604862No Hit
TGCAAATGGGATAAAATTCACTTGATGGATTTTGTAGTGATGTTTTCACAGT730.13962473461737085No Hit
CTACCGGGCAGTGTGTCACCATTTTAATTGGTGTCTATGACATACAGACAGG700.13388673182487618No Hit
GTGAATGGGCCATCGAACGAAGTAACCAAAACAGGAACTGAAAATGAAAATA690.13197406422737792No Hit
GATGTTTTTGTGGGGGGTTTCTGCTTTCCCTTGTTTCTTTTTCTTTCTTGCC680.1300613966298797No Hit
ACTCAAGGGTCTGTAGGTGGGCTGATCCACATGCGTGTGGCAGCCCAAGGCT670.12814872903238148No Hit
GAGCATCGGGAATGTCTTTCTGGGTAGCTTTAGGGGCATTTTGGTGGATTAG660.12623606143488322No Hit
CGCTTCGGGATTCTGAGGGGCCACAGTAATTACAAACTTACTATCCGCCATC650.124323393837385No Hit
TGTAAGGGATGTGCTAACATCTGTAGTTAAATTAGCCATTCTTTTTACATGA650.124323393837385No Hit
CTGTGTGTGGGATCTTTGTGTTTAGCTCATCCAAAGGTGTTACCCTGGTTTC640.12241072623988677No Hit
ACACAAGGGAACGACATAAATTGAAAAACACTAAATTAGAAAAATCAGGCAT640.12241072623988677No Hit
AACTCTGGGCTGAACGAATGAGCACACTTTAACCAGTTATTTACAGTTACCT640.12241072623988677No Hit
GTATACGGGAACCCCAGCCCAGTACCTTTCTACGTTTCTTCCCACTTTCCTC640.12241072623988677No Hit
TCAGCTTTTGGCCTCCTTGAGTAAATGACTTCTCCACTGCTGAATAGCCTAC630.12049805864238854No Hit
ATGGCTAGGGCTTGCTAAGTAGCAGTGTTAGTGTCTAATTCTGTATAGATTA630.12049805864238854No Hit
CTAATTGGGTGCATGTCAAATACTTTGTGATAGTTAAATAAGACTACAGGAC620.11858539104489031No Hit
ATTACAGGGTTAATCACTATGTAAGTGGTGAGAGATGCAGGACATACACATT620.11858539104489031No Hit
CATAATGGGCGCTCTTCCGATTTCAGCATGGGGGGCCCTCAGGGCTGGGGAG610.11667272344739207No Hit
GGCTAAGGGACAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCCTCC610.11667272344739207No Hit
TATCTTGGGTGAGAAGATGAAAGCATCTTTAGAAGGGCCACTTAAATTCACT610.11667272344739207No Hit
ACGGGGGTAGATCATGGAACAGGAAAAAGACATTAGCAGAAAAACTGGTGAA600.11476005584989385No Hit
GCCATTGGGACATTTAGAACCTGCATTTAAGAACAGAACAGCAAGTATGAAC600.11476005584989385No Hit
TGTTATGGGCGATCTGCCATGGGGAGTGCCCCAGAAAACATATTCTGTCAAA600.11476005584989385No Hit
ATGAAGGGGCAGTGGTGCCATCTCAGCTCACTGCAACCTCCACCTCCCAGGT590.11284738825239561No Hit
GCTAATGGGATATTTATAACTTTTCATATGCATTGAAATATATACATTAGCT590.11284738825239561No Hit
ATCTATGGGGGAAAAAAAGTTTCATCAAAGGGAAATTAGGGAAAAAAGTTTC590.11284738825239561No Hit
GATGTGGGGGCTGGGGATGAGAACTGAATAATCTTTGCCTGGAGTGACACTA590.11284738825239561No Hit
GGCTGGGATTTTATTAAATCAATGAAAAGTGATCAAAATGTTACCTGTCAGT590.11284738825239561No Hit
CTATCTGGGGCTTCCACGGCAGCATCTTCAGCTCTTGGGTTTAGCAAAAGTT590.11284738825239561No Hit
ACTTGTGGGAGACCCCCAAGACCATCATTCTTGCCCAGGCTTCAGAGACAAC590.11284738825239561No Hit
CGTTGTGGGGATCCTAAGGGAATTTTAGTTGACATTAGATCAGTTAATATTA580.11093472065489737No Hit
GAGTTAGGGACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCAAC580.11093472065489737No Hit
TGTAAAGGGATGGTTTGGCTGTGTCCCCACCAAAATCTCAACTTGAAATATA580.11093472065489737No Hit
AGTTATGGGAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTC580.11093472065489737No Hit
AATCTAGGGGTGAAAACCAAAATTAAAAACATAATAGTTTTTACAGTTGCTT570.10902205305739915No Hit
ACTCATGGGAGATATATGGTATCCATTTAGATGAAGGCTAGGAAGGAATCTT560.10710938545990091No Hit
AAAATCGGGGCTGTCTCTGCAAATTTAAATCCATCTTATTTCAGAATGCTGG550.1051967178624027No Hit
GCGCGCGGGCATTAGTTTGGATGAATTCAAGTCATTTTGCCATTTTACAACC550.1051967178624027No Hit
TTTTCGGGGGAGAGGAAGGGATTTGGAAACCATGTACTTTTGGGGAGTAATT550.1051967178624027No Hit
GTTAAAGGGATTTCAAAGAAGAACATTTTGATAAATATGATTATACGATATT550.1051967178624027No Hit
AATGTAGGGCGGAGTTCTGCTCCATATCCACCAGAAAGCTGGCGGCCCACGG550.1051967178624027No Hit
CTATCCGGGGAGAGATTCAGGGATAATCCACTGGACTTGGTGATAGACTAAA540.10328405026490448No Hit
ATAAGCGGGCTATTATGTACAAAACACCACAAAGCTTATGTAGGTAAGTGAT540.10328405026490448No Hit
CTGCTATCGGGACCCACGTCAATGGCATATTCTGGGAATCACCACCACCACC540.10328405026490448No Hit
TTTACGCGGGAGGTATGTAATTATTTGCTGCTGCTGCTGTGCTCTGTAAATT540.10328405026490448No Hit
TTCTCGCGGGGACTGGCACACAGGCGCTCTCATCCCGGCTCTCCTGGTGGGC540.10328405026490448No Hit
CATTTTGGGATCCAGTCAGCTGCATGGTACTGACTGAGCTGAGGAGTCAAAC540.10328405026490448No Hit
GTCTGCGGGATATAATGTTTTTATATAATGCTTAAGTAATTAAACAATTATA540.10328405026490448No Hit
AAAAGTGGGTAAGCCAATAGGGTTACAGGTTGGTACAGTGACACATTGAGAA530.10137138266740624No Hit
AGAACGGGGGATTTTAAACTTTACTATATTTGTATTCCTGTCTCCCCACCCC530.10137138266740624No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)