FastQCFastQC Report
Sat 31 Dec 2022
SRR5845256.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845256.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51022
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT3090.6056211046215358Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC2720.5331033671749441No Hit
TACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGG1070.20971345694014346No Hit
CTAATTGGGTGCATGTCAAATACTTTGTGATAGTTAAATAAGACTACAGGAC760.1489553525929991No Hit
TAGCGAGGGAATGAAACAGCTCCAATGCTGGAGCAGGCGATTTCACCTAAAC750.14699541374309122No Hit
GAGCATCGGGAATGTCTTTCTGGGTAGCTTTAGGGGCATTTTGGTGGATTAG710.1391556583434597No Hit
TCAGCTTTTGGCCTCCTTGAGTAAATGACTTCTCCACTGCTGAATAGCCTAC710.1391556583434597No Hit
AAAATCGGGGCTGTCTCTGCAAATTTAAATCCATCTTATTTCAGAATGCTGG670.13131590294382817No Hit
ACACAAGGGAACGACATAAATTGAAAAACACTAAATTAGAAAAATCAGGCAT670.13131590294382817No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTG670.13131590294382817No Hit
AACTCAGGGTTTCAGAGGAAGTTAAATGTCTTACAAATCCAATACTTTCTAA670.13131590294382817No Hit
GATGTTTTTGTGGGGGGTTTCTGCTTTCCCTTGTTTCTTTTTCTTTCTTGCC660.12935596409392028No Hit
CTATCCGGGGAGAGATTCAGGGATAATCCACTGGACTTGGTGATAGACTAAA650.1273960252440124No Hit
TCGCACGGGGGTGACTCGACGTGGTGAATGTGCACACGCCCTGCCTGGTCCG650.1273960252440124No Hit
GCTACGCGGGGAATTCTTGTGACTTCAAGAGTGATATTTTAGTAATTCTGTG640.1254360863941045No Hit
CATTTTGGGATCCAGTCAGCTGCATGGTACTGACTGAGCTGAGGAGTCAAAC640.1254360863941045No Hit
TGTAAGGGATGTGCTAACATCTGTAGTTAAATTAGCCATTCTTTTTACATGA640.1254360863941045No Hit
ATCTCGGGGGGACATTACTGAATTGTCTATTCATTTAGTACATTTTCTTAAC630.12347614754419661No Hit
TATCTTGGGTGAGAAGATGAAAGCATCTTTAGAAGGGCCACTTAAATTCACT630.12347614754419661No Hit
CTACCGGGCAGTGTGTCACCATTTTAATTGGTGTCTATGACATACAGACAGG620.12151620869428875No Hit
GGCTAAGGGACAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCCTCC620.12151620869428875No Hit
CTTGATGGGCACTGTGGCTGCCATGGTGGATGGAGGCTGGACAGAACCTGTG610.11955626984438085No Hit
TTTTCGGGGGAGAGGAAGGGATTTGGAAACCATGTACTTTTGGGGAGTAATT610.11955626984438085No Hit
TGCAGCGGGCTGAAACTGGTCTGGTGACAGATGCCTTCAAGCTGAAACGCAA600.11759633099447298No Hit
AACTCTGGGCTGAACGAATGAGCACACTTTAACCAGTTATTTACAGTTACCT600.11759633099447298No Hit
TTGGTGGGGGGGAAACTCTATCCATCCTGCCTTGAAGAGTCTCCATGGAAAC590.1156363921445651No Hit
CGTCAAGGGCGATCTAGTTCCGGGAGGAACTTAACACTTGTTTATGTCGATT590.1156363921445651No Hit
TCTGAAGGGTCAGCACTGAATATAATATCCTTTGTTCATGAATTGGAAGAAT590.1156363921445651No Hit
TATTAGGGAGCCAAACTAAGCTTCATAAGTGAAGGAGAAATAAAATCCTTTA590.1156363921445651No Hit
TTGGCTGGGTATGTATTGTTAAAACCTCTCAATTTTTTAAAGGCATCTGTGT590.1156363921445651No Hit
AATCCCGGGAAACCTGTGGTGCTTTCAAGCGTCAATGGCGGAACAGAATGAG590.1156363921445651No Hit
CAAAATGGGGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCT580.1136764532946572No Hit
CTAGGGAGGGATTGTGACAGCCCACACAGCGAGCCAGGGGCCATCGATGAAG580.1136764532946572No Hit
GCCTCGGCCTCCTGAGTGGCTGGGACTGCGGGTGTGCTCTACCACACCTGGC580.1136764532946572No Hit
CTATCTGGGGCTTCCACGGCAGCATCTTCAGCTCTTGGGTTTAGCAAAAGTT580.1136764532946572No Hit
TTGACTGGGTCTATAGACGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCT580.1136764532946572No Hit
CTTATGGGGGAGAAAACAATGGACAAAAGTACTGAAAAGACAAAAAAGGATA570.11171651444474932No Hit
TGTAAAGGGATGGTTTGGCTGTGTCCCCACCAAAATCTCAACTTGAAATATA570.11171651444474932No Hit
AGGTGGGGTAAAAGGGGATCTGAGAAATTATGTAATAGGGAGTGGGAATAAA570.11171651444474932No Hit
AATCTAGGGGTGAAAACCAAAATTAAAAACATAATAGTTTTTACAGTTGCTT570.11171651444474932No Hit
TTATATGGGATTGTCCTCTGAGCTGCAGTTTCCTCATCTGTCACATCGCTAC560.10975657559484144No Hit
GAGTTAGGGACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCAAC560.10975657559484144No Hit
GTATTCGGGATACCAAACATAATACTTTACATAAGAAAAGTGATGGTAACTT560.10975657559484144No Hit
ATTACTGGGACTGAATGTATATTCCAATATACGGACTCTTGATTTACACTCA550.10779663674493356No Hit
AGGCTAGGGATTTGCCTGTTTTGTTTGCTTTGTTTTCTTGTCATGAGATGGT550.10779663674493356No Hit
ACAAGTGGGGCACCAGGCCCCTCCGGACCAAACCAAAATGGAGTCACTCATA550.10779663674493356No Hit
TGCAAATGGGATAAAATTCACTTGATGGATTTTGTAGTGATGTTTTCACAGT550.10779663674493356No Hit
CAACGGGGGAGAATATTTGTGGAAGTCTTCCTTCTTTTGTCTTCAGGAAGAA550.10779663674493356No Hit
ATGGCTAGGGCTTGCTAAGTAGCAGTGTTAGTGTCTAATTCTGTATAGATTA550.10779663674493356No Hit
CTTATACCGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAG550.10779663674493356No Hit
ACTCAAGGGTCTGTAGGTGGGCTGATCCACATGCGTGTGGCAGCCCAAGGCT540.10583669789502569No Hit
CTTAGGGGGGGAAATTCTCATTCCTTGCTGCTGCAAGTGTGGAACAGTACAA540.10583669789502569No Hit
TGTTATGGGCGATCTGCCATGGGGAGTGCCCCAGAAAACATATTCTGTCAAA540.10583669789502569No Hit
TGTAAGGGGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATC540.10583669789502569No Hit
CTTCATGGGGACTTATTTTTCTTCCCCATTTAGTTTTTCCATGGTTTGGACT540.10583669789502569No Hit
GACAACGGGAGCAACTGCTCTCTGAACAGGATTCAAACTTAGATGTTACAAA540.10583669789502569No Hit
GCACGCGGGGGTCTTGCATTTCTTTCGAAAGCATCTCTCATCTCTAATAACA540.10583669789502569No Hit
AATAGGGGAGATAAGGGACATTCAACCTGTAATGTCAACTCAAATGTGCCAA540.10583669789502569No Hit
TGATTGGGGCTGACTCGGGGATTGTTACTCATATAATGGGATGATGGGACAA530.10387675904511778No Hit
GCTTGGGAGGCTGAGGCGGGAGGATTGCTTGGGCCCGGGAGGTGGAGGTTGC530.10387675904511778No Hit
TTAGGCGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC530.10387675904511778No Hit
TTTACAGGGATCTTATACGGGTACATTAAATCTTTAAGCACACAGACCAAGA530.10387675904511778No Hit
AGAACGGGGGATTTTAAACTTTACTATATTTGTATTCCTGTCTCCCCACCCC530.10387675904511778No Hit
ATACAGGGGGGACAGCCTTTGTCAAGACTGAAAATTCTCATGCTGCGCAAAG520.10191682019520991No Hit
AGCTCTGGGGACCAGCTGGGCATGCGCATGCAGCACAACCTGGAGCAGCAGA520.10191682019520991No Hit
GTGAATGGGCCATCGAACGAAGTAACCAAAACAGGAACTGAAAATGAAAATA520.10191682019520991No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)