FastQCFastQC Report
Sat 31 Dec 2022
SRR5845244.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845244.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123585
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCAAGCGATCTCGTATGC5530.4474653072783914No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCAAGCGATCTCGTATGCCGTCT5520.446656147590727RNA PCR Primer, Index 42 (95% over 21bp)
GCTTCCGGGAAACCTTTAAAACGATGGTAAGAAGAAAGTACTCTCATTGCAA1890.15293118096856415No Hit
CTGATAGGGGTCCAGCCTGGCTAAATGTTTTTAGACTTAAGAGATTAAAACA1650.13351134846461948No Hit
GATATGGGGGTGGAAAGCGCAAGGATAGAAGTAGAGAGGCTGATTAGGAGCA1620.13108386940162642No Hit
CGCTATGGGGCAGGCGGGAGTCTTCAGGATTTGTTAATAAAAGATAATCTTT1590.12865639033863333No Hit
GGTAATGGGGGAGGAGAAGGAACTTCTCATACAGCTGTTATTATTGATGAAA1560.12622891127564026No Hit
GCTTATGGGCCGATCTAACCAGGGGAACCTTGTCCTTGTAATTACATTCATT1520.1229922725249828No Hit
TTTGGAGGGATATTATCACATCTACACAGTATCTTCATAGCAACATCTAGAT1490.12056479346198973No Hit
TTACTATGGGAACCACTAAGTTAGATCTCTACCTCACAGTGTCCATACAAAT1480.11975563377432537No Hit
GCTGATTTCCCCTTACCAGCAGGTGGAGTGAGAATCTTCAGAGCTCTGGAGT1470.11894647408666101No Hit
GAAATTGGGCAGTATTTTAAAATTTTCAACAGTATTATAAAAACATTGCATC1470.11894647408666101No Hit
TGCATAGGGAATGAGCTAAAGACTCTAAAAGAGTAGCTGGTGCATATTAAAA1460.11813731439899663No Hit
ACAGGGACGTTGACAGTGTTGAAGCAGGGTGAATAACTAGGGCATATATATT1430.11570983533600356No Hit
GCATACGGGACACCACTGCACTCCAGCTTGGGTGACAGAGTGAGACTCCATT1420.1149006756483392No Hit
ATAGCGGGATGTCCATTCTGAGGGAACACTAGATATAAATGGCGTAACTCCC1420.1149006756483392No Hit
AAAAGAGGGGGCGATGATGCCAAGAAAGTGAAATGGGTGGACATTAATGATA1410.11409151596067484No Hit
CCAAGTGGGAATGCTTCAATTTTAAGTGGTAAGACATTTACTGGGACACAGA1410.11409151596067484No Hit
TACGTAGGGGTGGCTTTACATTTCAAAAGTGAACTTGATTCAACCTGAGAAA1400.11328235627301048No Hit
GAGGCTGGGGAATCCGGAAGTTAAGAGGAAATGTGTCATGTATGAATCTGGC1390.11247319658534612No Hit
CTCCACGGGTATAAGATTTATCTAGTGTATCTTTTGGGTTTTTGTAGTAAAG1380.11166403689768176No Hit
ATGCAAGGGGAGAGAGAAGTTAAAGACATGAGCCTTATTGTGGCTTGTTTGC1380.11166403689768176No Hit
CGTATAGGGACTCACCTTTCATGGTCAAGAGCTGCTCAGTGACACCAAACTG1370.1108548772100174No Hit
CGTGGCGGGCGCCCGCTTTGGCCTCCCGGGGTGCTGGGATTACAGGCGTGAG1350.10923655783468868No Hit
CCATAGGGGTCTACTGCAGGGGATAAATACAATTTTGACTTGAAATTTTATT1350.10923655783468868No Hit
ATGTATGGGAGGAGCGTGCCGTTTCCCCTGTAGATGGATACTTTTGTTTTGT1350.10923655783468868No Hit
CTTCAGGGGTAAAAATGGGAAACTGGAGAGAGAAAAAAAATTGTTTCAAGAA1340.10842739814702432No Hit
CAACCAGGGGACCATATCTGCAAATTGTAAGTACATACATTTTGATGACCTT1340.10842739814702432No Hit
CTCGGAGGGGCTTTTTTCCCTAACCAATATAGCTATCTTAAGTATCCTTAGG1330.10761823845935996No Hit
TCGCATGGGGTGACTGAAATGGCTTTCATCATCTTTAAGTCAAAGTGAGATG1320.1068090787716956No Hit
GCCGGGCAGGACCTTTTAATCTAATGACTCAGCTCTTCTGTGTCTCTGTTTT1310.10599991908403124No Hit
CTATACGGGGATCGGACCAAGTTACAGTGTTTGAAACAAGTCAGCAGAATTC1310.10599991908403124No Hit
GGTAATGGGGTAATGAAATGGAGGTACATGCATCAAGCAAGCACTTGACAAG1310.10599991908403124No Hit
AATTTAGGGGTGTGAAGAAACACTAGCTTATAAATTCTTAGTGAGATGAAAC1310.10599991908403124No Hit
ACTCGGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTAC1300.10519075939636688No Hit
CTTCAGGGATACACGCTTTGTCTGCAGGGAAGTTCTCCTGGTAATTACAAGG1300.10519075939636688No Hit
CAATGACGGGAGTATTTATAAGCTTTCTTTGACAACAATTCATAAGTTAAAA1300.10519075939636688No Hit
GGTTTCGGGTATGGAATTTGTTACGGGCCCTGGTCCATAATGGTGGCAGCCA1290.10438159970870252No Hit
ATTAATGGGCATCCTAAAAGTTAAAAACATGAAAAGCCGCCATCTTGTCATC1290.10438159970870252No Hit
TGAGGTGGGGTAGAACTAAGATCTTCTCTTCATTTCTCCCTTACAGTTGGCT1290.10438159970870252No Hit
TTGAGAGGGCTAGTTTTATCACATTAAGAGGCTCTCCATGCACAAAATGCAT1290.10438159970870252No Hit
GACTAGGGGGTGGTAAAAATGCACATGGTTAATGCACATGTTACTTTTAAAT1290.10438159970870252No Hit
GACTAGGGGTGGTATAAAAGAATAAAAGAACTTAAAATAAGTTAGCCCCCTG1290.10438159970870252No Hit
TAACTAGGGATTGAGTTATCAAAATATTAAAATTTGTGATTGGCAATGCGTG1280.10357244002103816No Hit
ATATTAGGGCTAAGAAGAAGGCCTTTACCAAGTACTGCAAGAAATGGCAGGA1280.10357244002103816No Hit
CTAATAGGGATATCTAACTGCATTCATTCCAATGTTTTTTATTTTTGTTTTT1280.10357244002103816No Hit
ATGCTGGGGTTACAGGTGTGAGCCACCATGCCTGGCTCAAAAAATTTTTTTC1280.10357244002103816No Hit
CATTAGGGGAAGGGAATTGCTTTCCAGATGCTAAGAAGCAGCAGTGGGGCTT1270.1027632803333738No Hit
TAGGAAAGAGAGTATGCGTGTCTGTGCACTACACATTCACAGAAAATCCTCT1270.1027632803333738No Hit
GAAATTGGGATATAATACTGTCATTTAAAGAAATACTTGAAAGCTTTAAAAA1260.10195412064570944No Hit
CAGAATGAAGATGTAGAGAAACAAGGAAGAAATTGACCCTGACGAAGAAGAA1250.10114496095804508No Hit
ATCTTTGGGAGGCAGTCCAAAAATCTACATGTGTCTCAATGCATACAATGAA1250.10114496095804508No Hit
AATCAGGGGCATAAGACTTTTTGAGTACTATGTTTGGGTGTGACCTTATTCT1240.10033580127038072No Hit
AACAGCGGGTAACACTTACATTTTATCCTGTAGATGTTGAAGAAAACACAGT1240.10033580127038072No Hit
CGTGTGGGGAGACAAGGTTCACAATATTCTTGCAGAAATGGTGATGGGGGGA1240.10033580127038072No Hit
AACTGGGGAGACAAAATTAATCTAGGATGGAAAATATCACAGTGGTGGTTGC1240.10033580127038072No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)