FastQCFastQC Report
Sat 31 Dec 2022
SRR5845240.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845240.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161578
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCAAGCGATCTCGTATGC5620.34781962890987633No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCAAGCGATCTCGTATGCCGTCT4500.2785032615826412RNA PCR Primer, Index 42 (95% over 21bp)
GTTGAGGGGCTGTAACTTGGGTTTCAATAATTATGGATTTTTATGAAAACTT2080.12873039646486528No Hit
AATAGTGGGGGAGGCGCAGCGGTCGCGTTAGTTCGGCCCAATGGCGGCACCG2000.12377922737006276No Hit
CACACGGGGCGGCCGTAGCAGCCGGGCTGGTCCTGCTGCGAGCCGGCGGCCC1990.12316033123321245No Hit
TGCGAGGGAAGGTACTGTGATTGCTCACATGATTTTTGGTTCTTATGAAGGT1980.12254143509636212No Hit
GTCGCGGGGCAGCTTCACTTGTAATAGTCTTAAACTGGAAACAACCCAATCT1900.11759026600155961No Hit
TCTTGTGGGGGTTCTCTCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCAT1880.116352473727859No Hit
TAGAGTGGGATAAGAAATCTGAATAGTTCTCCCCTTTGAGTGCATTTAACTC1870.11573357759100869No Hit
ATGAATGGGCCAGGAGACGGAGGTTTCAGTGAATGAAGATCGTGCCATTGTA1850.11449578531730804No Hit
GAGACAGGGAAGAAAAGACTGAAGGAGAAAGGAAAGAGGGAGTAAGGAAGGA1850.11449578531730804No Hit
AACGTCGGGGGCCAGTGATGATGAGCATTTTTTCATGTGTCTGTTGGCTGCA1830.11325799304360742No Hit
TTCCTTGGGGCCTACCTGGAATTTAAAATTCAGTAGACAAATTAATTGGTCC1820.11263909690675711No Hit
GCTATTGGGTATATGAGAATTTTAACCAATGATATCTGTTTTTTTTCTAGCT1810.1120202007699068No Hit
TCACATGGGCTGAAGGATTATAAAATCATTGGTTGTAAATATTAACCAGCTC1810.1120202007699068No Hit
TTAGCCGGGCCTCCCAAAGTGCTGTGATTACCAATACCACTTTATTTATAAA1800.11140130463305649No Hit
GAGCCCGGGCTGACTTAATTGGTTCTATGTGGAGCCCAGAATTTGGAATTCT1780.11016351235935584No Hit
CAGAGAAGGGATTTGTGAATTTGTTGTTCAGCTTTTCATTCAAACAAAAAAT1770.10954461622250554No Hit
GCTTTAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1750.10830682394880492No Hit
AAGTATGGGACTATTTCTACTTGGCCAGGTGTGGGCTCAAGAGCCTTACTCT1740.1076879278119546No Hit
GACATCGGGATAACTTCTCAATTCTGACATTATACTGTGAAAGCAAATATAT1740.1076879278119546No Hit
TATTGTGGGACTTCAGGTCTTCATTAGTATATTACAGGAAAATCAAAGACTA1730.10706903167510429No Hit
AATAGCTGGGATTACAGGCACACCACCTCCACGCCCAGCTAATTTTTTGTAT1720.10645013553825396No Hit
TTTCATGGGACAACGATATGGCCGGAGACCATATGGTGTTGGTCTCCTTATT1710.10583123940140365No Hit
TTCGGCGGGTGGCACTAAGTGTTTTCATTGTAGGATCAGCAGCAGGTTAAAG1710.10583123940140365No Hit
GTGAATGGGGAATTTTATTTTGGGTGTAGATCACTTTAAGCGAACCTGATTT1710.10583123940140365No Hit
AGCAGCGGGATCAGGATTTTTGTTTACTGAAAGACTCTAATCAAGCTTACTA1710.10583123940140365No Hit
AGCTAAGGGGAATTCCTTTGGAACTAATAAATATACAGTTATGGGAAAAGTA1710.10583123940140365No Hit
GTTCAGGGGATTTAATTTAATGGATGAAAACTATCTGAATCGATTGTATTTA1710.10583123940140365No Hit
ACCACAGGGTAAGGACTCACATAAACTTAAGGTAAAGGAGTAGAAAAACATT1700.10521234326455334No Hit
TCACGCCGTTTTCCCGCCCGTTGGTGGATGCGTGTGAGCCGTTTTCCTGGCT1700.10521234326455334No Hit
GCCTTAGGGAGGGAAGACAGGAAGCCCCAATGGGGCAACCAGAGCAGCCCAA1690.10459344712770303No Hit
GTAAAGGGGATTTGAGTCTCAGGGTGACAAAGTTGTGCAATCCCTTTTGGTG1670.10335565485400242No Hit
GTTTGCGGGATTGGGAGTTCTGGCTGGGGCAATCAGGCAAGAAAAAGAAATA1670.10335565485400242No Hit
GCACCCGGTTCCATCTTAATACTTTTTATATTGATTCTATGTTGAAATGATA1660.10273675871715207No Hit
AACAATGGGAGTTTTATTTGTGTGTGTCATTCCATTTTTACATTTAAAAATA1660.10273675871715207No Hit
CATCTTGGGGTATGGAAGGGAATAACACGAGACTGACTTAGAAGGTTTCCCT1660.10273675871715207No Hit
GCGCTGGGGATGACATTGAACATTGGAGACCTTGACTCTCAGTTAGCAACTG1650.10211786258030177No Hit
AGACGAGGGGATCAGAAGCAGGTAGCCAATGGGAAGAGCACTTCTGAGTGAT1650.10211786258030177No Hit
TCGGGTGGGGGCAGCAGGCGGGCCCCCGCGCGGCAGGGCCCTGGACCCGCGC1640.10149896644345145No Hit
CCTTCAGGGGGACTTCGGTTGAGGAAACGGGTATTTCATGTCTCAGGGAGTA1640.10149896644345145No Hit
ACACCACGCCCGGCTAATTTTTGTATTTTTGGAGGAGATGGGATTTCGCTAT1630.10088007030660115No Hit
AGTGCTGGAGGACAGCTGTTGAGATAAAAAAATTCGTTGTCATTTATTCCCA1630.10088007030660115No Hit
TATGCCGGGCATTGTTGTCTTGTTTTAAGAAATTGCCGTAGCCACCCAAACC1630.10088007030660115No Hit
AGTAGGGAATGAAATCAACCCGAGTGGAATGGAACGGAATGGAATGGCATGG1630.10088007030660115No Hit
CGCACGGGGATTTATGCATTTATGCATGAGAACTAAATAGATTTTTAGATTC1620.10026117416975083No Hit
GACCGTGGGGAAATTCTGAAGACTCTTAATAACTCCCCGACAGGTTTTATTG1620.10026117416975083No Hit
CTCGATGGGGGAAGAGGGGAACAGAAAACTTAGGCAGTTTCTTTAAATAAAC1620.10026117416975083No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)