FastQCFastQC Report
Sat 31 Dec 2022
SRR5845239.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845239.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162097
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCAAGCGATCTCGTATGC5280.3257308895291091No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCAAGCGATCTCGTATGCCGTCT4650.2868652720284768RNA PCR Primer, Index 42 (95% over 21bp)
TTAGCCGGGCCTCCCAAAGTGCTGTGATTACCAATACCACTTTATTTATAAA2340.14435800785949154No Hit
AATAGTGGGGGAGGCGCAGCGGTCGCGTTAGTTCGGCCCAATGGCGGCACCG2130.13140280202594742No Hit
AGACGAGGGGATCAGAAGCAGGTAGCCAATGGGAAGAGCACTTCTGAGTGAT2050.12646748551793063No Hit
GAGACAGGGAAGAAAAGACTGAAGGAGAAAGGAAAGAGGGAGTAAGGAAGGA1970.12153216900991382No Hit
TTCCTTGGGGCCTACCTGGAATTTAAAATTCAGTAGACAAATTAATTGGTCC1960.12091525444641173No Hit
GTTGAGGGGCTGTAACTTGGGTTTCAATAATTATGGATTTTTATGAAAACTT1950.12029833988290962No Hit
CAGAGAAGGGATTTGTGAATTTGTTGTTCAGCTTTTCATTCAAACAAAAAAT1950.12029833988290962No Hit
AATAGCTGGGATTACAGGCACACCACCTCCACGCCCAGCTAATTTTTTGTAT1940.11968142531940751No Hit
ACCACAGGGTAAGGACTCACATAAACTTAAGGTAAAGGAGTAGAAAAACATT1880.11597993793839492No Hit
GTCGCGGGGCAGCTTCACTTGTAATAGTCTTAAACTGGAAACAACCCAATCT1880.11597993793839492No Hit
TTAAACGGGAAGTTTTTGTAGACTAAATTGCCCTCTCTACTTTGCATCGTTT1850.11412919424788862No Hit
AGTAGGGAATGAAATCAACCCGAGTGGAATGGAACGGAATGGAATGGCATGG1830.11289536512088441No Hit
GCGCTGGGGATGACATTGAACATTGGAGACCTTGACTCTCAGTTAGCAACTG1820.1122784505573823No Hit
AAGTATGGGACTATTTCTACTTGGCCAGGTGTGGGCTCAAGAGCCTTACTCT1820.1122784505573823No Hit
AGTGCTGGAGGACAGCTGTTGAGATAAAAAAATTCGTTGTCATTTATTCCCA1800.1110446214303781No Hit
GCTACAGGGCAATAAAAATACCTTTAACGAAGAGTTTCTATGTGTCAGGTAC1800.1110446214303781No Hit
TCGGCCGGGATATGGAAATACGAAATTGCAAATCTAAGTGAAAAACCTTTGT1790.110427706866876No Hit
GTGAATGGGGAATTTTATTTTGGGTGTAGATCACTTTAAGCGAACCTGATTT1780.10981079230337391No Hit
AGAGGGGGAGGACAAAAAGGCCCTTTAAACTAGGGAAGAAGAGTGAAACGGA1760.1085769631763697No Hit
CTCGATGGGGGAAGAGGGGAACAGAAAACTTAGGCAGTTTCTTTAAATAAAC1760.1085769631763697No Hit
CCTTCAGGGGGACTTCGGTTGAGGAAACGGGTATTTCATGTCTCAGGGAGTA1750.10796004861286759No Hit
ACGTCGGGGATCTAGTGCCGGGGCATTTTAAAATTAAAGACAGGATCTTGCT1750.10796004861286759No Hit
CATAGCGGGCAAAGCATAAAAGCAGAAAAAAATCTGAGATTAGTATCTGATG1750.10796004861286759No Hit
TAGAGTGGGATAAGAAATCTGAATAGTTCTCCCCTTTGAGTGCATTTAACTC1730.1067262194858634No Hit
GACATCGGGATAACTTCTCAATTCTGACATTATACTGTGAAAGCAAATATAT1730.1067262194858634No Hit
CATCTTGGGGTATGGAAGGGAATAACACGAGACTGACTTAGAAGGTTTCCCT1710.10549239035885921No Hit
GCTATTGGGTATATGAGAATTTTAACCAATGATATCTGTTTTTTTTCTAGCT1690.104258561231855No Hit
ATGAATGGGCCAGGAGACGGAGGTTTCAGTGAATGAAGATCGTGCCATTGTA1690.104258561231855No Hit
GCACCCGGTTCCATCTTAATACTTTTTATATTGATTCTATGTTGAAATGATA1690.104258561231855No Hit
TATGCTGGGCTGCAACTTGAGTGATTTTACATAGAAAGAAATGGGCATTCCT1690.104258561231855No Hit
TTACAGGGGGATCTTAAATCGGGCTGAGCTGGGGCAAAGATGGTGATGGAAC1690.104258561231855No Hit
TGTCACGGGATTCCAGTTAATCATGTGATTGACAAGTAACAGAACTAAAACT1680.10364164666835289No Hit
ATCTCTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1680.10364164666835289No Hit
GACCGTGGGGAAATTCTGAAGACTCTTAATAACTCCCCGACAGGTTTTATTG1680.10364164666835289No Hit
ACACCACGCCCGGCTAATTTTTGTATTTTTGGAGGAGATGGGATTTCGCTAT1670.1030247321048508No Hit
GTGACTGGTTCAAATGCATTTATTTCCCAGAAGCAGAATAGTAAGAGTGTGC1670.1030247321048508No Hit
TTCGGCGGGTGGCACTAAGTGTTTTCATTGTAGGATCAGCAGCAGGTTAAAG1670.1030247321048508No Hit
CTGTCCGGGCTGTAGTTAGACACTATGTCTCTAAATTGATCTTTTTAATTAA1670.1030247321048508No Hit
AACGTCGGGGGCCAGTGATGATGAGCATTTTTTCATGTGTCTGTTGGCTGCA1660.1024078175413487No Hit
TCACATGGGCTGAAGGATTATAAAATCATTGGTTGTAAATATTAACCAGCTC1660.1024078175413487No Hit
CACACGGGGCGGCCGTAGCAGCCGGGCTGGTCCTGCTGCGAGCCGGCGGCCC1650.1017909029778466No Hit
GCTTCAGGGACAGAGGGAATTCGAACAGTTTCCTTCAAGATAAAATAATGAA1650.1017909029778466No Hit
TCTTGGGGGACAGAGAGAGGTGGTAACTATATTACCAGGAATTCCAAGTATG1640.10117398841434448No Hit
GTTCAGGGGATTTAATTTAATGGATGAAAACTATCTGAATCGATTGTATTTA1640.10117398841434448No Hit
AGAAAGGGGAGGCGAGATGATACATTTTCTGTTACAATGCTTGGTCATTGCA1630.10055707385084241No Hit
AGCAGCGGGATCAGGATTTTTGTTTACTGAAAGACTCTAATCAAGCTTACTA1630.10055707385084241No Hit
GCCGTGGGATCACATATAGTTAGAATGGTTGAGGAATGCTTTGTTTAAAAAA1630.10055707385084241No Hit
AGTATTCCCGGAAATATCATCTTATTATTATTATTTTCTAAATCTAATATCA1630.10055707385084241No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)