FastQCFastQC Report
Sat 31 Dec 2022
SRR5845238.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845238.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161640
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCAAGCGATCTCGTATGC5010.3099480326651819No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCAAGCGATCTCGTATGCCGTCT4480.27715911902994306RNA PCR Primer, Index 42 (95% over 21bp)
GCTACAGGGCAATAAAAATACCTTTAACGAAGAGTTTCTATGTGTCAGGTAC2050.12682504330611236No Hit
GTTGAGGGGCTGTAACTTGGGTTTCAATAATTATGGATTTTTATGAAAACTT2020.12496906706260826No Hit
AATAGTGGGGGAGGCGCAGCGGTCGCGTTAGTTCGGCCCAATGGCGGCACCG1990.12311309081910418No Hit
TTAGCCGGGCCTCCCAAAGTGCTGTGATTACCAATACCACTTTATTTATAAA1980.12249443207126949No Hit
GCGCTGGGGATGACATTGAACATTGGAGACCTTGACTCTCAGTTAGCAACTG1960.1212571145756001No Hit
TGCGAGGGAAGGTACTGTGATTGCTCACATGATTTTTGGTTCTTATGAAGGT1940.12001979707993071No Hit
AGCAGCGGGATCAGGATTTTTGTTTACTGAAAGACTCTAATCAAGCTTACTA1930.11940113833209601No Hit
GTCGCGGGGCAGCTTCACTTGTAATAGTCTTAAACTGGAAACAACCCAATCT1910.11816382083642663No Hit
GACATCGGGATAACTTCTCAATTCTGACATTATACTGTGAAAGCAAATATAT1880.11630784459292255No Hit
AGACGAGGGGATCAGAAGCAGGTAGCCAATGGGAAGAGCACTTCTGAGTGAT1870.11568918584508785No Hit
CACACGGGGCGGCCGTAGCAGCCGGGCTGGTCCTGCTGCGAGCCGGCGGCCC1850.11445186834941846No Hit
AAGTATGGGACTATTTCTACTTGGCCAGGTGTGGGCTCAAGAGCCTTACTCT1840.11383320960158376No Hit
TCACATGGGCTGAAGGATTATAAAATCATTGGTTGTAAATATTAACCAGCTC1830.11321455085374907No Hit
TCGGCCGGGATATGGAAATACGAAATTGCAAATCTAAGTGAAAAACCTTTGT1810.11197723335807969No Hit
ACGTCGGGGATCTAGTGCCGGGGCATTTTAAAATTAAAGACAGGATCTTGCT1810.11197723335807969No Hit
GGTTGCGGGTACAATTCTTTATTAAAGTTAACAGAAAGGAACTGATCGTTTG1790.1107399158624103No Hit
CCTTCAGGGGGACTTCGGTTGAGGAAACGGGTATTTCATGTCTCAGGGAGTA1770.10950259836674092No Hit
CAAACAGGGTGGGACAATGTACTCTTCTCATTAATGAAGCATCTAAAACACC1770.10950259836674092No Hit
GCCTTAGGGAGGGAAGACAGGAAGCCCCAATGGGGCAACCAGAGCAGCCCAA1770.10950259836674092No Hit
AGTGCTGGAGGACAGCTGTTGAGATAAAAAAATTCGTTGTCATTTATTCCCA1730.10702796337540212No Hit
AATAGCTGGGATTACAGGCACACCACCTCCACGCCCAGCTAATTTTTTGTAT1720.10640930462756744No Hit
CATAGCGGGCAAAGCATAAAAGCAGAAAAAAATCTGAGATTAGTATCTGATG1720.10640930462756744No Hit
CATCTTGGGGTATGGAAGGGAATAACACGAGACTGACTTAGAAGGTTTCCCT1720.10640930462756744No Hit
AGTAGGGAATGAAATCAACCCGAGTGGAATGGAACGGAATGGAATGGCATGG1700.10517198713189806No Hit
AGAAAGGGGAGGCGAGATGATACATTTTCTGTTACAATGCTTGGTCATTGCA1680.10393466963622866No Hit
TTCCTTGGGGCCTACCTGGAATTTAAAATTCAGTAGACAAATTAATTGGTCC1680.10393466963622866No Hit
GGCTGCGGGCACTAAACATAATGACTGAAAAAAATTATTATTATAAATCACC1680.10393466963622866No Hit
AACAATGGGGAAGTGTCCTTCTGTCTTGGCCTCCCAAGGTGCTGAGATTTGA1680.10393466963622866No Hit
GCTTTAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1670.10331601088839396No Hit
CATCTAGGGACATCTGAGGTATCCAATTTTTCAGCCTGACTCTAACACACCA1660.10269735214055928No Hit
GAGCCCGGGCTGACTTAATTGGTTCTATGTGGAGCCCAGAATTTGGAATTCT1660.10269735214055928No Hit
TTCGCAAGGGAGAGTTGAACTGAAGAAAATCGAGACATAAAAAAGCATTCAA1650.10207869339272457No Hit
TCTTGTGGGGGTTCTCTCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCAT1640.10146003464488987No Hit
CACATAGGGATAGCCGGTGACACCCTTCCCGTATCTTCAGGGCAGCTGCAGC1640.10146003464488987No Hit
TTGCTGCAGGGATGTCAATAACTTTTATGCAAGACACAATACCTTGCAAACT1620.10022271714922049No Hit
AGTGCTGGGAATCTGGAATTCCACCAATCCTCTAAGACAAAGTTTCATAAAC1620.10022271714922049No Hit
TTCGATGGGAAGCCCTAAGTGGTTAATTTTTATCTTACCAGCAGATACCCCC1620.10022271714922049No Hit
CAGAGAAGGGATTTGTGAATTTGTTGTTCAGCTTTTCATTCAAACAAAAAAT1620.10022271714922049No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)