FastQCFastQC Report
Sat 31 Dec 2022
SRR5845222.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845222.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26539
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCAAGCGATCTCGTATGC2911.0964994913146688No Hit
TCGTCAGGGATACTATTAAAAGTAAAGTCAGTGCAGAAAATCACTTACGGAA2400.9043294773729229No Hit
TGTAAAGGGTATCCAGGATGACTGTGTTGCTGCAGTCTTTGAGTGGTTGGAG2390.900561437883869No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCAAGCGATCTCGTATGCCGTCT2380.8967933983948151RNA PCR Primer, Index 42 (95% over 21bp)
GGTAGGGGGAGAAAAAAGTACATGTGAAATACTACTACTTATCCATCTCCTG2290.8628810429933305No Hit
ATGCTGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC2260.8515769245261691No Hit
CACATCAGGGAACCAGGTGGTAGATATAATCAAATTGTTTTGCAGAATCAAT2220.8365047665699535No Hit
CAATGTGGGGACGCTCTTCCGATCTCTCTATGGGTACTGTTTCCTTATCAAA2180.8214326086137382No Hit
GTAGGGGCTGGATCTCCGTATTTAAAGTTGTAATTCCACTTTACTGTTTTCC2100.7912882927013075No Hit
TTACGCGGGAGTATCTCTCCTTTCAGACTGTTTTCACATGTACAGGCGCATA2080.7837522137231998No Hit
TTTATTGGGGGTGGAGAATATCAAGGATCCTTTGTTCAGGTTCTTTGAGAGA2040.7686800557669844No Hit
TCTATGGGGAGTAGGCTGGGCGGGGAGCGTCGGACCAGAAGAGAATCACGCC2000.753607897810769No Hit
CATTGAGGGATGAAGGATGAGGAACTGAGGCGCATGCAGGAGATGCTGCAGA1960.7385357398545537No Hit
CATCCAGGGAAACCCTGTATGTGTTTATATGTGCTTCACAGAGTTCGTGTCA1900.7159275029202306No Hit
ACGGTAGGGGGCTGTGAAAACAGTTTTTCTTTTGCTTGTTTCTTCCTGAGTT1870.704623384453069No Hit
AGATAAGGGATCCATTTTTTATTATTTTCTTAGTTTTGTTCTTGGACAAATT1860.7008553449640152No Hit
TACGAAGGGTCACTGATGTAGTACCATATCCAATATCTCTGAGATGGAATTC1840.6933192659859075No Hit
TACGCAGGGGTAATAAGGAGATTTTTCTTTCCAAAGCAATTCACAAGTCCTT1840.6933192659859075No Hit
AAACAAGGGGGGAGGGAGCGCGCCAGGCCTGGCGCGCACCCGACCCCTTGCG1830.6895512264968537No Hit
GGAATAGGGGATCTAATATCGGGGAAAATGGGCTGAAATAGAAGAAATTGAT1810.6820151475187459No Hit
TTTGTTGGGGATACATTTATTTAAAATTCCTTGTGGACTCTGGATATTAGAC1800.6782471080296922No Hit
TACTAAGGGTCATCATATCTGAGTTCATGCTAATGAGGTAACTATTGTTGGG1760.6631749500734768No Hit
ATTTTGGGGCCAGGCTGAGTCCATACTGCCCCTGATCCCAGAAGGAATGCTG1700.6405667131391537No Hit
CTACGTGGGCTACTTAAAAATAAAGACCAACAAAAAATATAGTTGTTTTTAA1690.6367986736500998No Hit
AGTCCTGGGAGATGTGAAACAATTGAAATTTTCATACATTGCTGATAGAAAT1680.633030634161046No Hit
GCACATGGGATCATCATTTGCTTCAAGCCCCGAAACACAGCAGAGCTCCAGC1670.6292625946719922No Hit
AGTAGTGGGAACTTTTAATAGTTTTGTGTATCATTCAACTTTTTTTCTTGCA1630.6141904367157768No Hit
GCACTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1530.5765100418252384No Hit
ATTTCGGGGTTCAGTTCACGGCGATAAAGAAGTAGGTGCTGCACACAAATAT1530.5765100418252384No Hit
GTGTAAGGGGGACCATTGTGGATTGCTCTTAGCTATTTTCATGTCAGATGCA1530.5765100418252384No Hit
GTCCTGAGGGCACGGTATTATTGTTTCCTCAGAAGAGTGTGTATTTAAATAC1500.5652059233580767No Hit
GTTATAGGGTATATGTTGGGAAACTTCTTAAGGTTGGAGAATGAACCACTCA1490.5614378838690229No Hit
GTCGGTGGGCAGAGGACGAGGAAACAATCGTGGCTACAAAAATCAATCTCAG1430.5388296469346998No Hit
CATAATGGGAACAGTGTCTCTCTTTCAACGCTATCATGGAAGAACTGGGAAT1420.535061607445646No Hit
AGGCCCGGGTGTCTTACTGCTAGCTCTTCTAACAGGGGAAGTCTGTATGAAT1400.5275255284675383No Hit
AACCCGGGGTTACAGGAAGGCGATCTTGTTGGGCTCAGCGTTACGTGTATCT1390.5237574889784845No Hit
GCCCAGGGTGGTGCCATCCGAGAATTTGCAGAGCACATTTGCCTACTAATGG1360.512453370511323No Hit
GCTTCTGGGAAGTTTTAAATCACAATCTGGAGAATTTTCTATGATTAGGAAG1350.5086853310222691No Hit
CGCAACGGGTGCCACGACCCGGGGAGTTTATCATGCGGCTTGTGTGTTTCAG1330.5011492520441615No Hit
GCTCAGGGGGGTAGATTTGCAATATAGTCTGTGAAAAACAATAACAACAACA1290.4860770940879461No Hit
ATGGCCGGGACCTCAAGGAACTAGAGAAGAGCAAACTGAGCACAAATGTAGT1250.47100493613173067No Hit
TAATGTGGGCAGCCCAGTCCTGCATGTTTGAAAGCATCAGCTGCTTGAAAAA1220.45970081766456916No Hit
CTGTTTGGGCATGGCTCATTGCTTACTGTGATTCTGCTCTTTTTTTCTTTCT1190.44839669919740754No Hit
GTCCCTGGGGTGCTGGGATTACAGGTGTGAGCCACCGTGCCCAGCTAGATTC1170.4408606202192999No Hit
CCATCAGGGATGCTCATTTTCGGGGCTTCCTTGCCAGGGCTCCCACTTCCTG1170.4408606202192999No Hit
GCTTATGGGATACATTCCATTATTAAATTCTGCCCTATTCCCAATTGTTAGC1160.43709258073024604No Hit
AGAAGGGGGTTTTATAGTTTTAGAATATTAAAACCTCAGATATTTATGTGGG1090.4107163043068691No Hit
AATATTGGGGGGCCCTCATTTGGCCAATAGCATCCTTTTCCAGTCTTCCGGT1080.40694826481781526No Hit
GCTAGTGGGGGTATGTTTCTGGTCGGAGGTGTCATTTGTGGTTTATGGCCAT1050.39564414635065376No Hit
GTGCTGGGTTATGGCAATATTCGAGCTGTGGCTAGCAGATCAGCAGATCGAT1040.3918761068615999No Hit
AAATGCGGGTAATTTGAGGTGGAAGTGGAAAGTGGAAACAACAATTATGAAA1010.3805719883944384No Hit
AAAACAGGGATTCACATTTTATATAAACAACCTACCAAACAAAAGTACTTCC1000.3768039489053845No Hit
AGTTGGGGGAAGGTCCCAATTTCCTCGAATAAATAGGCAAACAAGAACTAAT1000.3768039489053845No Hit
CAGTTGGGGATGTTTATCAAATCGTGTACTAAAATGGAAAGCTAGTTTTGAG970.365499830438223No Hit
GTTTCAGGGATGCTTGGAGCATTCTCATTCGAGAGGCACAGGTTTAGTGATA970.365499830438223No Hit
AACTAGGGATTAATTAATCACATTATTTTCTGCCCTGAAATCCCAAAATACT970.365499830438223No Hit
GCAGAGTGGGCACACAGAGCTGTTGGGAAACCTCAAAGCAGTAAATCAAGCA960.36173179094916913No Hit
AAGTTAGGGCAAGCACAAATCTTTCCCACTAGCCATTTAATAAGTTAAAAAA940.3541957119710615No Hit
CTACCCGGGGGGAGGCCAAGGCAGGCAGATCACCTGAGCCCAGGAGTTCGAG940.3541957119710615No Hit
TACTACGGGGATCTAAGCGAGGGAGGATCGCTTGGGCCCAGGAGGTAGAGGT940.3541957119710615No Hit
AGAGTTGGGATTGTTTGTTAAAAATGTTTTGAAATTATTAATGTGACTATGA940.3541957119710615No Hit
GCTCCGGGGAGTGCAGTATAGACATATATTTGTACATCTTTCATGTGCCTTA930.3504276724820076No Hit
AGCGCGGGTGTTATCGTCAGGAGCTGTGCAAACTGATGTGTGAACGGCTAAA920.34665963299295377No Hit
ATCACTGGGGTATCAGGAAATCTTTACTGAGAGACAGGAAAAGATAGAGCAG910.3428915935038999No Hit
CTCTCCGGGAACAGAAGTTTATTTTCTCATAGTTCTGGAGGCTGGACATCCA900.3391235540148461No Hit
CTTCTCGTGGGTGACTTTAGCTGCATTTCACATATTTTAATATGTACACTTC890.3353555145257922No Hit
GGCTACGGGTGGACAAGGTCTGATACATTGTCTTTGGATGAACAGGCTTCAT870.32781943554768456No Hit
AGAAACGGGATTGCCTTTAAAAATCTGTTCCGTTTTTTTCCCAGGTACTTAA850.32028335656957685No Hit
GCGTACGGGAAGGTAATCTACCTTGGGATTACTAGATCATATGGTTTTTTAA840.316515317080523No Hit
GGTTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC830.31274727759146914No Hit
AAACATCGGGCTCTGTAAATAGTTGTTATACTATATTGTTTAGGGAATAATG830.31274727759146914No Hit
AGAACTGGGAAGCTTTCTGAATTGTACCTATGTCAGTTTCCAGGTTTTGATA830.31274727759146914No Hit
GCTATAGGGGTGTAATAGCCCAGCCAGTGGTTGAAATGAAGTACCTGCCCAG820.30897923810241534No Hit
CATAGTGGGCGGTGCTGCTGGGCCTCCAGTAAATCTTATCCCCAGCTGCTTC800.30144315912430764No Hit
ATTTGCGGGTGATTAAGTTAGCAGTTAAAAAATATAACTTGTGTAGCCAGAA800.30144315912430764No Hit
GCTACTGGGGTAGCTGGCACTACTGAACAGCTTTAACTCTTCCAGACTTTCA780.29390708014619993No Hit
TATAGCAGGGAAGTGTGTGTGTCTGTGTGTGTGTGTGCACGTGCGTGTGTAT780.29390708014619993No Hit
AGCCTGGGCAACAGAGTAAGACAAAAAAATATATATATATTGAAAATCAAAG770.2901390406571461No Hit
GCATATGGGCACACACAGAAAATCCTAAAAAATACAAACCAATAAAGCAACT770.2901390406571461No Hit
TGGTCTGGGACATCATTCACTTGGAAGGCGTGGTCACTAAATGTAAACCAGT760.2863710011680923No Hit
TGCTTTGGGACATATAATATATACATGTACATATAAAGTATTTAAAATAAAG750.28260296167903837No Hit
TTGTTAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG750.28260296167903837No Hit
ACAACAGGGAATACAACTTGGTTATGTGGAAACATGTTGCTCATAATAAATG740.27883492218998457No Hit
ACGCATGGGTGTAGATAATTGTTACACTATATTATTTAGGGAATCATGACAA730.2750668827009307No Hit
GCGAACGGGACCCCTTCCTCTTTCTCTCTCCTTTGAATCATCTCAATTTTAT690.2599947247447153No Hit
TGCATTGGGAGAAGTAAGGTGTATAAAACCATTGAGTACTAATAGACTGCGG680.2562266852556615No Hit
TTAATAGGGTGGTAGGAAATAGATAATGATGTTTTTGACATTCAGTGGCATC670.2524586457666076No Hit
GTGTTGGGGCGATCTGTCATTGGGGTCGACTGACGGTAACGGGGCAGAGAGG670.2524586457666076No Hit
TTCTCTGGGAAAGTGTTATGGGATTCCCTAGTGAGGAAAGAATTGGTATCTC650.2449225667884999No Hit
GCCAAAGGGACCGGGCTCTTGATGGACTTGGCAGCCAATGAAAAGGCCGTTC650.2449225667884999No Hit
GTATGGGGTTAGGAAAATAGGCACACTTTCAAAGAGAATGTTCTCTTACACA640.2411545272994461No Hit
CATAGGGTAAACATGATAGTCCATAACCATTTTGAAATGCTGGGCAAACTAC630.2373864878103923No Hit
GAGTGGGGTGCAATGACAACTTGTTTCTGTTTTATTTAAAGTAACTGACATT630.2373864878103923No Hit
GTTGATGGGGGCCCGGCTGAGCCGACATTGCCGGCGTCTTGGCGATTCGGCC620.2336184483213384No Hit
TGCGCGGTGGCTGGGGATGCCTCACAATCATGACGGAAGGCAAGGAGGAACA620.2336184483213384No Hit
CGATAGGGGATAATTAAATTTCCTGAAAGAGTATTGTACTTTCTGAACCCAC610.22985040883228458No Hit
GCCCGCCTCAACTTCCGGAAGTGCTGAGATTGCAGGTGTGAGCCACTGCACC610.22985040883228458No Hit
AACACGGGGCGATCTACGAGAGGGATGTGATCTCCAAGTTAAAAAAAATTTT600.22608236934323073No Hit
TGTTCAGGGTCGACCATCCATTTTTCTTTCTTATCAGAAACAGGAGAACTGG600.22608236934323073No Hit
CTACTGGGGTGACCAAACCAGCCTTCGGAGCGTTCTCTGTCCTACTTCTGAC600.22608236934323073No Hit
ATTTATGGGACCCAAGAATCTACAACGATGATAAGAACACCTATATTCGTTA590.2223143298541769No Hit
AATTGTGGGAACCTGACTAACTGATGAAAGTGTTTCTCTCCTAAGGCCAGTC590.2223143298541769No Hit
CGAATAGGGATGTTTTCATAGTGCTGAAATCTTTAATATTCCTTAATTTGAA580.21854629036512302No Hit
CACACAAGGGATAAGATCTTTGTGATGGCAACTCATGGCTTGTTGTCTTCTG570.2147782508760692No Hit
TGCAGAGGGTCAGCCATATTAATATACATTGACATAAAGACCTTTGTTTTAA560.21101021138701534No Hit
ACCCAAGGGTGACTCAATGTAAATAAATCAATAAACATGATAAGCATCTTCA550.2072421718979615No Hit
GTTAATGGGAGCCTACCCCTTCCTTGTACTATCCCTATGAGGCATAATTATA550.2072421718979615No Hit
TAATGCGGGATGGTTAGTCAGTTATTATAAGTTACTCTTAAAATGTTTTATT540.20347413240890763No Hit
TAATACGGGATGGAATATTTTATCCAAGTCCTTTGTTATCATTAAATTATTC540.20347413240890763No Hit
AATCCAGGGTATGCATGTTCATTGCAACTTAAAAGCGTAAAAACCCCAAATG540.20347413240890763No Hit
TTCCCCTTGGGCATGGCCCAGCCAGGGGTCTCTGTCAAGTCCCTGGTGTCGT530.1997060929198538No Hit
GTTAATGGGAACCTCACCACCTCTTGCTCAGCCTATATACCGCCATCTTCAG510.1921700139417461No Hit
ATTATTGGGAATGAAATATCTTAAGTCTAGGGTACTTTTTCCTAGAATCCTG500.18840197445269224No Hit
ATTTAAGGGATGGTAAGGCTACAATACTCAAGTAACAAGGTTTGGGACAATG500.18840197445269224No Hit
CTCACCAGGGGACTTCAAGGGGAAGCTTGATGGCAGCTCCTCTGATGATATT500.18840197445269224No Hit
TTAGTCGGGGGGTTTCACCATGTTGACCAGGCTGGTCTCGAACTCCTGACCC490.18463393496363842No Hit
GAGGCGGGAGGAGCGCTTGAGCCCAGGAGTTTGAGGACAGCCTGGGCCGCAT480.18086589547458456No Hit
GCATCATGGGAAGACCAGCCTGGCCAACATGGTGAAACCCCGTCTCTACTAA470.17709785598553074No Hit
TAGCGTGGGGTTTATAATACACCCAAAGGATCAGAAACATCCCAGTGTCATT460.17332981649647689No Hit
ATGGCTGGGCACGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAA450.16956177700742306No Hit
GACCGGGAGAACTGAATAACTGCAGTGACTTGATGCTCTAAAACAGTGTAGG450.16956177700742306No Hit
TAGCAAGGGGTTCCTGATTCCATTTAGATCTTTATTGTTTTTCTTCATTTTT450.16956177700742306No Hit
CATGAGGGTATAACCTATTTTATTCTCTGCCAAACAATTTTATGACTGCTGT440.1657937375183692No Hit
GAGTTCCGGGGTCTCGCATGGAAGAAGTCGATTAGGTTAGGAGTTCATAGTT440.1657937375183692No Hit
AACTTTGGGGCCATTTTAAATGCAAAGCAAGACTTGTGAATCATAGTGTCTC430.16202569802931535No Hit
CAATGCGGGCAAACAGCGGGCTGATAGCAAGGAGAGCCTCAAAGCCACTACC430.16202569802931535No Hit
AGTTTGGGGATTAGAAATTATTTGATCAATGCTTCATTTCATTAAAATATCA420.1582576585402615No Hit
TCTGCAGGGATCTCTCCTTTCAATGATTAAAATTACCCATAGCTTCTAGAAA410.15448961905120767No Hit
TCACTGGGGATAGCTGAAAGGTGAAAGCAACACAGAGGTCCATCAACTGATA400.15072157956215382No Hit
TTCTATGGGGTGAATAACCTCCTGTAAATGAATTTTATAACAAAAATGTACT380.14318550058404614No Hit
TTAGCAGGGATATACTTGAATGCAACATTGTGTTTTATTTTCCAACTCTGCC380.14318550058404614No Hit
CATAAAGGGGGGCATGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGC380.14318550058404614No Hit
GGCACAGGGGAGTAGCAAAGGATCGAAGGCGACTGTCGCTACAACCCTCCCC370.13941746109499228No Hit
GGATGGGGGCAAGAAATAGGAAACCCCCCAATGTCTGTTGAACAGCTATCAC370.13941746109499228No Hit
AGATAAGGGATTAAAGTTTTGAACCAAATGTGTTAAAGCTTACGCTTTGCCA370.13941746109499228No Hit
TTCATGGGGTAGTAGAGAGAGGGTTTCACCGTGTTGGCCGGGCTGGTCTTGA370.13941746109499228No Hit
CGATAAGGGATTGTCCCTACTCTCCAACCCACTGTCCTCAACCCTTATAGCA360.13564942160593843No Hit
ATCATGGGGGGCAGTCACAGACACTTGAGCACACGCGTACACCCAGACATCT360.13564942160593843No Hit
CTATAAGGGTGCTTATAGCACTTTAAGTTATTCTAAAAATGAAATTATAAGC360.13564942160593843No Hit
GGGCCTGGGGGGGGACCTTGGAATGTATCCCCTGTGAATAATGGGGGCTACT350.13188138211688458No Hit
TGTTTTGGGGAGTATTACTTTGTACAGGTTGATCACTTTTTTTAGAGTGAAG350.13188138211688458No Hit
GCATATGGGGGGCCTGGTAATTAAAGATTGACCCCTGACCTAATTGGTTATG340.12811334262783075No Hit
AATAGTGGGGGTTTCCTAAGGATACACTTTAGATATCAGACTGAGACAGAGG340.12811334262783075No Hit
TGCCATGGGAGCTTGAATTTATTTTATTTATTTATTTTTCATGGTGGGGGGC340.12811334262783075No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCAAGCGAACTCGTATGC340.12811334262783075No Hit
CCAGAGGGACTACAGGACCAGAAGAAGCATTTCGAAATGTATGCCTGGCATC330.1243453031387769No Hit
TACGAGGGGTAGCTATTTTTCTTGTTGCATCTTTTCTACTTCAGTACACTTG320.12057726364972304No Hit
TATTTAGGGGCAGCTTTTAAAAGATTTTTTTTTTTTCTCTCAACACCATGAT320.12057726364972304No Hit
TTCAGCGGGTCTGCAATACTGTCTTTAATGTATACTCACTTGTTATAGAAGC310.1168092241606692No Hit
AACAGGGGGGCCTGCTTGATGGATACAGAGTTTCCTTTGGGGGCGATGAAAG290.10927314518256151No Hit
TGCGTGAGGGATTGAGGAAAAAGAGCGTGGCTTATTCATCTTTCAATCTTTT290.10927314518256151No Hit
TGCGCTGGGCGCCTGTAATCCTAGCACTGTGGGAGGCTAAGGCAGGCGGATA290.10927314518256151No Hit
GATATCGGGTAAGTCGATGTTGGGAAACGTTTTAACATCTGGAGCCTTTGTG290.10927314518256151No Hit
CTAATTGGGATCAACGGGTACAAACGAGTCCTGGCCTTGTCTGTGGAGACGG280.10550510569350767No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGCAAGCG280.10550510569350767No Hit
GGCAAAGGGGGCTGGAGGAGTCCCAGTGTAATAAGGTCCCGGAGAAGTGTCA270.10173706620445382No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)