FastQCFastQC Report
Sat 31 Dec 2022
SRR5845221.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845221.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26695
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCAAGCGATCTCGTATGC3061.1462820752949991No Hit
CAATGTGGGGACGCTCTTCCGATCTCTCTATGGGTACTGTTTCCTTATCAAA2691.0076793407005058No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCAAGCGATCTCGTATGCCGTCT2681.0039333208466004RNA PCR Primer, Index 42 (95% over 21bp)
ACGGTAGGGGGCTGTGAAAACAGTTTTTCTTTTGCTTGTTTCTTCCTGAGTT2410.9027907847911594No Hit
TCGTCAGGGATACTATTAAAAGTAAAGTCAGTGCAGAAAATCACTTACGGAA2360.8840606855216333No Hit
TTACGCGGGAGTATCTCTCCTTTCAGACTGTTTTCACATGTACAGGCGCATA2350.880314665667728No Hit
CATTGAGGGATGAAGGATGAGGAACTGAGGCGCATGCAGGAGATGCTGCAGA2270.8503465068364863No Hit
TGTAAAGGGTATCCAGGATGACTGTGTTGCTGCAGTCTTTGAGTGGTTGGAG2140.8016482487357183No Hit
GGTAGGGGGAGAAAAAAGTACATGTGAAATACTACTACTTATCCATCTCCTG2120.7941562090279078No Hit
AGTCCTGGGAGATGTGAAACAATTGAAATTTTCATACATTGCTGATAGAAAT2090.7829181494661922No Hit
ATGCTGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC2070.7754261097583817No Hit
TACTAAGGGTCATCATATCTGAGTTCATGCTAATGAGGTAACTATTGTTGGG2060.7716800899044765No Hit
CATCCAGGGAAACCCTGTATGTGTTTATATGTGCTTCACAGAGTTCGTGTCA2000.7492039707810452No Hit
AGATAAGGGATCCATTTTTTATTATTTTCTTAGTTTTGTTCTTGGACAAATT2000.7492039707810452No Hit
TACGAAGGGTCACTGATGTAGTACCATATCCAATATCTCTGAGATGGAATTC1930.7229818318037086No Hit
TCTATGGGGAGTAGGCTGGGCGGGGAGCGTCGGACCAGAAGAGAATCACGCC1930.7229818318037086No Hit
TTTATTGGGGGTGGAGAATATCAAGGATCCTTTGTTCAGGTTCTTTGAGAGA1910.7154897920958981No Hit
GTAGGGGCTGGATCTCCGTATTTAAAGTTGTAATTCCACTTTACTGTTTTCC1900.7117437722419928No Hit
CTACGTGGGCTACTTAAAAATAAAGACCAACAAAAAATATAGTTGTTTTTAA1870.7005057126802772No Hit
GGAATAGGGGATCTAATATCGGGGAAAATGGGCTGAAATAGAAGAAATTGAT1790.6705375538490355No Hit
ATTTTGGGGCCAGGCTGAGTCCATACTGCCCCTGATCCCAGAAGGAATGCTG1770.6630455141412249No Hit
CACATCAGGGAACCAGGTGGTAGATATAATCAAATTGTTTTGCAGAATCAAT1760.6592994942873197No Hit
AGTAGTGGGAACTTTTAATAGTTTTGTGTATCATTCAACTTTTTTTCTTGCA1760.6592994942873197No Hit
TACGCAGGGGTAATAAGGAGATTTTTCTTTCCAAAGCAATTCACAAGTCCTT1720.6443154148716987No Hit
GCACATGGGATCATCATTTGCTTCAAGCCCCGAAACACAGCAGAGCTCCAGC1680.6293313354560779No Hit
ATTTCGGGGTTCAGTTCACGGCGATAAAGAAGTAGGTGCTGCACACAAATAT1600.5993631766248361No Hit
GCCCAGGGTGGTGCCATCCGAGAATTTGCAGAGCACATTTGCCTACTAATGG1560.5843790972092152No Hit
AGGCCCGGGTGTCTTACTGCTAGCTCTTCTAACAGGGGAAGTCTGTATGAAT1540.5768870575014048No Hit
AAACAAGGGGGGAGGGAGCGCGCCAGGCCTGGCGCGCACCCGACCCCTTGCG1460.546918898670163No Hit
TAATGTGGGCAGCCCAGTCCTGCATGTTTGAAAGCATCAGCTGCTTGAAAAA1450.5431728788162578No Hit
GTCCTGAGGGCACGGTATTATTGTTTCCTCAGAAGAGTGTGTATTTAAATAC1450.5431728788162578No Hit
GCACTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1410.5281887994006368No Hit
GTGTAAGGGGGACCATTGTGGATTGCTCTTAGCTATTTTCATGTCAGATGCA1400.5244427795467316No Hit
CGCAACGGGTGCCACGACCCGGGGAGTTTATCATGCGGCTTGTGTGTTTCAG1310.49072860086158454No Hit
GCTTCTGGGAAGTTTTAAATCACAATCTGGAGAATTTTCTATGATTAGGAAG1300.4869825810076793No Hit
AAAACAGGGATTCACATTTTATATAAACAACCTACCAAACAAAAGTACTTCC1290.4832365611537741No Hit
CATAATGGGAACAGTGTCTCTCTTTCAACGCTATCATGGAAGAACTGGGAAT1280.47949054129986884No Hit
AACCCGGGGTTACAGGAAGGCGATCTTGTTGGGCTCAGCGTTACGTGTATCT1280.47949054129986884No Hit
GCTCAGGGGGGTAGATTTGCAATATAGTCTGTGAAAAACAATAACAACAACA1220.4570144221764375No Hit
AGAAGGGGGTTTTATAGTTTTAGAATATTAAAACCTCAGATATTTATGTGGG1220.4570144221764375No Hit
TTTGTTGGGGATACATTTATTTAAAATTCCTTGTGGACTCTGGATATTAGAC1220.4570144221764375No Hit
GTCGGTGGGCAGAGGACGAGGAAACAATCGTGGCTACAAAAATCAATCTCAG1220.4570144221764375No Hit
GTCCCTGGGGTGCTGGGATTACAGGTGTGAGCCACCGTGCCCAGCTAGATTC1190.4457763626147218No Hit
GCTAGTGGGGGTATGTTTCTGGTCGGAGGTGTCATTTGTGGTTTATGGCCAT1180.44203034276081665No Hit
CTACCCGGGGGGAGGCCAAGGCAGGCAGATCACCTGAGCCCAGGAGTTCGAG1150.43079228319910096No Hit
GTTATAGGGTATATGTTGGGAAACTTCTTAAGGTTGGAGAATGAACCACTCA1150.43079228319910096No Hit
AATATTGGGGGGCCCTCATTTGGCCAATAGCATCCTTTTCCAGTCTTCCGGT1140.42704626334519574No Hit
GCTATAGGGGTGTAATAGCCCAGCCAGTGGTTGAAATGAAGTACCTGCCCAG1130.4233002434912905No Hit
CTTCTCGTGGGTGACTTTAGCTGCATTTCACATATTTTAATATGTACACTTC1120.41955422363738526No Hit
GCAGAGTGGGCACACAGAGCTGTTGGGAAACCTCAAAGCAGTAAATCAAGCA1110.4158082037834801No Hit
AAATGCGGGTAATTTGAGGTGGAAGTGGAAAGTGGAAACAACAATTATGAAA1100.4120621839295748No Hit
ATGGCCGGGACCTCAAGGAACTAGAGAAGAGCAAACTGAGCACAAATGTAGT1100.4120621839295748No Hit
GCTCCGGGGAGTGCAGTATAGACATATATTTGTACATCTTTCATGTGCCTTA1100.4120621839295748No Hit
CCATCAGGGATGCTCATTTTCGGGGCTTCCTTGCCAGGGCTCCCACTTCCTG1090.4083161640756696No Hit
AAACATCGGGCTCTGTAAATAGTTGTTATACTATATTGTTTAGGGAATAATG1080.4045701442217644No Hit
GTTTCAGGGATGCTTGGAGCATTCTCATTCGAGAGGCACAGGTTTAGTGATA1060.3970781045139539No Hit
AGAGTTGGGATTGTTTGTTAAAAATGTTTTGAAATTATTAATGTGACTATGA1060.3970781045139539No Hit
AGTTGGGGGAAGGTCCCAATTTCCTCGAATAAATAGGCAAACAAGAACTAAT1040.3895860648061435No Hit
CTGTTTGGGCATGGCTCATTGCTTACTGTGATTCTGCTCTTTTTTTCTTTCT1030.3858400449522382No Hit
GCTTATGGGATACATTCCATTATTAAATTCTGCCCTATTCCCAATTGTTAGC1030.3858400449522382No Hit
AACTAGGGATTAATTAATCACATTATTTTCTGCCCTGAAATCCCAAAATACT1020.382094025098333No Hit
CTCTCCGGGAACAGAAGTTTATTTTCTCATAGTTCTGGAGGCTGGACATCCA1010.3783480052444278No Hit
AGCGCGGGTGTTATCGTCAGGAGCTGTGCAAACTGATGTGTGAACGGCTAAA950.3558718861209964No Hit
TACTACGGGGATCTAAGCGAGGGAGGATCGCTTGGGCCCAGGAGGTAGAGGT940.35212586626709125No Hit
GGCTACGGGTGGACAAGGTCTGATACATTGTCTTTGGATGAACAGGCTTCAT930.348379846413186No Hit
ATCACTGGGGTATCAGGAAATCTTTACTGAGAGACAGGAAAAGATAGAGCAG900.33714178685147034No Hit
AGAACTGGGAAGCTTTCTGAATTGTACCTATGTCAGTTTCCAGGTTTTGATA870.32590372728975464No Hit
TGCTTTGGGACATATAATATATACATGTACATATAAAGTATTTAAAATAAAG860.32215770743584937No Hit
CACACAAGGGATAAGATCTTTGTGATGGCAACTCATGGCTTGTTGTCTTCTG860.32215770743584937No Hit
CAGTTGGGGATGTTTATCAAATCGTGTACTAAAATGGAAAGCTAGTTTTGAG850.3184116875819442No Hit
GCATATGGGCACACACAGAAAATCCTAAAAAATACAAACCAATAAAGCAACT840.31466566772803894No Hit
TGCATTGGGAGAAGTAAGGTGTATAAAACCATTGAGTACTAATAGACTGCGG820.3071736280202285No Hit
AGCCTGGGCAACAGAGTAAGACAAAAAAATATATATATATTGAAAATCAAAG810.3034276081663233No Hit
GTGTTGGGGCGATCTGTCATTGGGGTCGACTGACGGTAACGGGGCAGAGAGG780.2921895486046076No Hit
TGTTCAGGGTCGACCATCCATTTTTCTTTCTTATCAGAAACAGGAGAACTGG770.2884435287507024No Hit
AAGTTAGGGCAAGCACAAATCTTTCCCACTAGCCATTTAATAAGTTAAAAAA760.2846975088967972No Hit
TTGTTAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG760.2846975088967972No Hit
GGTTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC750.2809514890428919No Hit
GTATGGGGTTAGGAAAATAGGCACACTTTCAAAGAGAATGTTCTCTTACACA750.2809514890428919No Hit
GCGAACGGGACCCCTTCCTCTTTCTCTCTCCTTTGAATCATCTCAATTTTAT740.2772054691889867No Hit
CATAGTGGGCGGTGCTGCTGGGCCTCCAGTAAATCTTATCCCCAGCTGCTTC740.2772054691889867No Hit
GTGCTGGGTTATGGCAATATTCGAGCTGTGGCTAGCAGATCAGCAGATCGAT730.2734594493350815No Hit
GTTGATGGGGGCCCGGCTGAGCCGACATTGCCGGCGTCTTGGCGATTCGGCC720.26971342948117627No Hit
ACAACAGGGAATACAACTTGGTTATGTGGAAACATGTTGCTCATAATAAATG710.265967409627271No Hit
ATTTGCGGGTGATTAAGTTAGCAGTTAAAAAATATAACTTGTGTAGCCAGAA680.25472935006555536No Hit
CGATAGGGGATAATTAAATTTCCTGAAAGAGTATTGTACTTTCTGAACCCAC670.2509833302116501No Hit
ACCCAAGGGTGACTCAATGTAAATAAATCAATAAACATGATAAGCATCTTCA660.2472373103577449No Hit
TATAGCAGGGAAGTGTGTGTGTCTGTGTGTGTGTGTGCACGTGCGTGTGTAT660.2472373103577449No Hit
ACGCATGGGTGTAGATAATTGTTACACTATATTATTTAGGGAATCATGACAA640.23974527064993442No Hit
GCTACTGGGGTAGCTGGCACTACTGAACAGCTTTAACTCTTCCAGACTTTCA640.23974527064993442No Hit
GAGTGGGGTGCAATGACAACTTGTTTCTGTTTTATTTAAAGTAACTGACATT630.23599925079602924No Hit
AGAAACGGGATTGCCTTTAAAAATCTGTTCCGTTTTTTTCCCAGGTACTTAA620.232253230942124No Hit
CATGAGGGTATAACCTATTTTATTCTCTGCCAAACAATTTTATGACTGCTGT620.232253230942124No Hit
TTCTCTGGGAAAGTGTTATGGGATTCCCTAGTGAGGAAAGAATTGGTATCTC620.232253230942124No Hit
TTAATAGGGTGGTAGGAAATAGATAATGATGTTTTTGACATTCAGTGGCATC610.22850721108821875No Hit
GCCCGCCTCAACTTCCGGAAGTGCTGAGATTGCAGGTGTGAGCCACTGCACC610.22850721108821875No Hit
GCCAAAGGGACCGGGCTCTTGATGGACTTGGCAGCCAATGAAAAGGCCGTTC610.22850721108821875No Hit
TTCCCCTTGGGCATGGCCCAGCCAGGGGTCTCTGTCAAGTCCCTGGTGTCGT590.22101517138040833No Hit
TGGTCTGGGACATCATTCACTTGGAAGGCGTGGTCACTAAATGTAAACCAGT590.22101517138040833No Hit
ATTTATGGGACCCAAGAATCTACAACGATGATAAGAACACCTATATTCGTTA590.22101517138040833No Hit
CTACTGGGGTGACCAAACCAGCCTTCGGAGCGTTCTCTGTCCTACTTCTGAC590.22101517138040833No Hit
AATTGTGGGAACCTGACTAACTGATGAAAGTGTTTCTCTCCTAAGGCCAGTC570.21352313167259787No Hit
TGCAGAGGGTCAGCCATATTAATATACATTGACATAAAGACCTTTGTTTTAA570.21352313167259787No Hit
TAATGCGGGATGGTTAGTCAGTTATTATAAGTTACTCTTAAAATGTTTTATT560.20977711181869263No Hit
AACACGGGGCGATCTACGAGAGGGATGTGATCTCCAAGTTAAAAAAAATTTT560.20977711181869263No Hit
TTCTATGGGGTGAATAACCTCCTGTAAATGAATTTTATAACAAAAATGTACT560.20977711181869263No Hit
GCATATGGGGGGCCTGGTAATTAAAGATTGACCCCTGACCTAATTGGTTATG560.20977711181869263No Hit
GAGTTCCGGGGTCTCGCATGGAAGAAGTCGATTAGGTTAGGAGTTCATAGTT560.20977711181869263No Hit
AATCCAGGGTATGCATGTTCATTGCAACTTAAAAGCGTAAAAACCCCAAATG550.2060310919647874No Hit
GCGTACGGGAAGGTAATCTACCTTGGGATTACTAGATCATATGGTTTTTTAA540.2022850721108822No Hit
TAGCGTGGGGTTTATAATACACCCAAAGGATCAGAAACATCCCAGTGTCATT540.2022850721108822No Hit
ATTTAAGGGATGGTAAGGCTACAATACTCAAGTAACAAGGTTTGGGACAATG520.19479303240307175No Hit
TCTGCAGGGATCTCTCCTTTCAATGATTAAAATTACCCATAGCTTCTAGAAA520.19479303240307175No Hit
CAATGCGGGCAAACAGCGGGCTGATAGCAAGGAGAGCCTCAAAGCCACTACC520.19479303240307175No Hit
CATAGGGTAAACATGATAGTCCATAACCATTTTGAAATGCTGGGCAAACTAC510.1910470125491665No Hit
CGAATAGGGATGTTTTCATAGTGCTGAAATCTTTAATATTCCTTAATTTGAA500.1873009926952613No Hit
GTTAATGGGAGCCTACCCCTTCCTTGTACTATCCCTATGAGGCATAATTATA500.1873009926952613No Hit
GCATCATGGGAAGACCAGCCTGGCCAACATGGTGAAACCCCGTCTCTACTAA490.18355497284135605No Hit
CTATAAGGGTGCTTATAGCACTTTAAGTTATTCTAAAAATGAAATTATAAGC480.17980895298745084No Hit
TGCGCGGTGGCTGGGGATGCCTCACAATCATGACGGAAGGCAAGGAGGAACA480.17980895298745084No Hit
TTAGCAGGGATATACTTGAATGCAACATTGTGTTTTATTTTCCAACTCTGCC470.17606293313354562No Hit
TCACTGGGGATAGCTGAAAGGTGAAAGCAACACAGAGGTCCATCAACTGATA470.17606293313354562No Hit
CCAGAGGGACTACAGGACCAGAAGAAGCATTTCGAAATGTATGCCTGGCATC450.16857089342573517No Hit
GGGCCTGGGGGGGGACCTTGGAATGTATCCCCTGTGAATAATGGGGGCTACT440.16482487357182993No Hit
GTTAATGGGAACCTCACCACCTCTTGCTCAGCCTATATACCGCCATCTTCAG440.16482487357182993No Hit
TTAGTCGGGGGGTTTCACCATGTTGACCAGGCTGGTCTCGAACTCCTGACCC430.16107885371792469No Hit
ATGGCTGGGCACGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAA420.15733283386401947No Hit
TTCATGGGGTAGTAGAGAGAGGGTTTCACCGTGTTGGCCGGGCTGGTCTTGA420.15733283386401947No Hit
TAATACGGGATGGAATATTTTATCCAAGTCCTTTGTTATCATTAAATTATTC420.15733283386401947No Hit
TAGCAAGGGGTTCCTGATTCCATTTAGATCTTTATTGTTTTTCTTCATTTTT410.15358681401011426No Hit
TGTTTTGGGGAGTATTACTTTGTACAGGTTGATCACTTTTTTTAGAGTGAAG400.14984079415620902No Hit
CTCACCAGGGGACTTCAAGGGGAAGCTTGATGGCAGCTCCTCTGATGATATT400.14984079415620902No Hit
AACTTTGGGGCCATTTTAAATGCAAAGCAAGACTTGTGAATCATAGTGTCTC390.1460947743023038No Hit
TATTTAGGGGCAGCTTTTAAAAGATTTTTTTTTTTTCTCTCAACACCATGAT370.13860273459449335No Hit
GGCACAGGGGAGTAGCAAAGGATCGAAGGCGACTGTCGCTACAACCCTCCCC360.13485671474058814No Hit
TGCTAAGGGATAAAATAGACAAATAGAAAATGGTCCAATTAGTTTCCTTTTT360.13485671474058814No Hit
ATTATTGGGAATGAAATATCTTAAGTCTAGGGTACTTTTTCCTAGAATCCTG340.12736467503277768No Hit
CACTAAGGGAGCCTAGCATTTCCTAGAGAAGAAAAATTGTCAGCATTTTCTT340.12736467503277768No Hit
AGATTCGGGTGGTTTTATCTTGGCTTTTAGTAATCATGTTGGCTGGTCTGGT330.12361865517887245No Hit
GGATGGGGGCAAGAAATAGGAAACCCCCCAATGTCTGTTGAACAGCTATCAC320.11987263532496721No Hit
CGATAAGGGATTGTCCCTACTCTCCAACCCACTGTCCTCAACCCTTATAGCA310.116126615471062No Hit
TCAACCGGGTGATCATTTGTTCAGAAAAAAAGCCCCTGAACTGATTTTGTTA310.116126615471062No Hit
ATGCGAGGGTGACCTGTGTTATACTTCCTATTTTTAATGGGCTTTTATGATG300.11238059561715678No Hit
CATAAAGGGGGGCATGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGC300.11238059561715678No Hit
AACAGGGGGGCCTGCTTGATGGATACAGAGTTTCCTTTGGGGGCGATGAAAG290.10863457576325154No Hit
TAAGGTGGGGAGATGTTGGATCGTTATGAATATCAAATGTCCATTTCTATTG290.10863457576325154No Hit
AGTTTGGGGATTAGAAATTATTTGATCAATGCTTCATTTCATTAAAATATCA290.10863457576325154No Hit
GCAGCTGGGGGACCACAGGCACGCATCGCCGTGCCTGGCTAATCTTTACGTT290.10863457576325154No Hit
GATATCGGGAGAGTGGCTGGGGTTTGAACCTTTAGGATTTCACAGCCATGCT280.10488855590934631No Hit
CAAGCCGGGTAACACTGGTGAATTTGGATAAGGAGCAAATTCAGATTGTATG280.10488855590934631No Hit
TGTGCAGGGGCGTAGCGGGATTGTTTCTGTTACTAACATTAAAAGTGTGCAA280.10488855590934631No Hit
GACCGGGAGAACTGAATAACTGCAGTGACTTGATGCTCTAAAACAGTGTAGG280.10488855590934631No Hit
AGATAAGGGATTAAAGTTTTGAACCAAATGTGTTAAAGCTTACGCTTTGCCA270.1011425360554411No Hit
GAGGCGGGAGGAGCGCTTGAGCCCAGGAGTTTGAGGACAGCCTGGGCCGCAT270.1011425360554411No Hit
TGCGCTGGGCGCCTGTAATCCTAGCACTGTGGGAGGCTAAGGCAGGCGGATA270.1011425360554411No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)